Sharp eyespot(Rhizoctonia cerealis)is a widespread soil-borne fungal disease that poses a severe threat to wheat health,and it is one of the main obstacles to achieving stable and high-quality wheat yields in China.Ou...Sharp eyespot(Rhizoctonia cerealis)is a widespread soil-borne fungal disease that poses a severe threat to wheat health,and it is one of the main obstacles to achieving stable and high-quality wheat yields in China.Our collaborative team has developed a novel,efficient,and low-toxicity fungicide named Y17991(N-(2-(2,4-bis-(trifluoromethyl)phenoxy)phenyl)-3-(difluoromethyl)-1-methyl-1H-pyrazole-4-carboxamide).Preliminary laboratory tests confirmed the significant inhibitory effect of this agent on R.cerealis.Large-area field trials also demonstrated its efficacy,with a disease prevention index of 83.52%,which is 1.97%greater than that of the widely used thifluzamide,and it significantly increased the wheat yield.Moreover,this study explored the impacts of Y17991 on the structure and function of the microbial community in wheat rhizosphere soil.Bacterial communities were more strongly affected than fungal communities.Y17991 significantly modulated key amino acid metabolic pathways and certain biosynthetic processes in diseased wheat rhizospheres,and it also enhanced certain biosynthetic pathways and metabolic activities in healthy wheat rhizospheres.Additionally,the application of Y17991 regulated rhizosphere metabolites,thus exerting significant control over the microbial community.We identified 15 microbial strains potentially involved in the prevention and treatment of R.cerealis,and Y17991 treatment promoted the growth of Pedobacter and Bacillus strains.These strains not only aid in plant growth but they also have the potential for disease prevention.In summary,Y17991 application at a reasonable dose does not cause significant disruption to nontarget rhizosphere microbial communities.In future studies,we will continue to investigate the impacts of Y17991 on nonmicrobial components in soil ecosystems,such as protozoa and nematodes.Our research provides a theoretical basis for the scientific application and promotion of new fungicides and offers a significant reference for establishing a comprehensive system for assessing the ecological impact of pesticides on the environment.展开更多
Gallium oxide(Ga2O3) thin films were prepared on Si substrate by magnetron sputtering. The obtained samples were comprehensively characterized by X-ray photoelectron spectroscopy(XPS) and scanning electron microscope(...Gallium oxide(Ga2O3) thin films were prepared on Si substrate by magnetron sputtering. The obtained samples were comprehensively characterized by X-ray photoelectron spectroscopy(XPS) and scanning electron microscope(SEM). Ti, Pt, Ni and AZO were deposited on the Ga2O3 thin films as electrodes. This paper mainly studies the metal-semiconductor contact formed by these four materials on the films and the influence of annealing at 500℃ on the metal-semiconductor contact. The I-V characteristics show a good linear relationship, which indicates ohmic contact between Ga2O3 and other electrodes.展开更多
Objective: To investigate the influence of hyperbaric oxygen combined with psychotropic drugs on serum neurotransmitter and cytokine contents in patients with post-stroke depression. Methods: A total of 62 patients wi...Objective: To investigate the influence of hyperbaric oxygen combined with psychotropic drugs on serum neurotransmitter and cytokine contents in patients with post-stroke depression. Methods: A total of 62 patients with post-stroke depression who were treated in the hospital between January 2015 and February 2017 were divided into routine group (n=31) and hyperbaric oxygen group (n=31) by random number table method. Routine group received antidepressant therapy, hyperbaric oxygen group received hyperbaric oxygen combined with antidepressant therapy, and the therapies lasted for 8 weeks. The differences in the serum contents of monoamine neurotransmitters, inflammatory factors and oxidative stress indexes were compared between the two groups before and after treatment. Results: There was no statistically significant difference in serum levels of monoamine neurotransmitters, inflammatory factors and oxidative stress indexes between the two groups before treatment. After 8 weeks of treatment, serum monoamine neurotransmitters NE, 5-HT and DA contents of hyperbaric oxygen group were higher than those of routine group;serum inflammatory factors IL-1, IL-6, IL-8 and hs-CRP contents were lower than those of routine group;serum oxidative stress indexes LHP and MDA contents were lower than those of routine group whereas CAT and GSH-Px contents were higher than those of routine group. Conclusion:Hyperbaric oxygen combined with psychotropic drug therapy can effectively increase the expression of monoamine neurotransmitters and balance the homeostasis in patients with post-stroke depression.展开更多
目的探讨辅助生殖技术(assisted reproductive technology,ART)助孕女性经济毒性的现状及其影响因素,为制订针对性干预措施提供依据。方法2024年9-12月,采用便利抽样法选取湖南、广西两省3所三级甲等医院生殖中心就诊的314例ART助孕女...目的探讨辅助生殖技术(assisted reproductive technology,ART)助孕女性经济毒性的现状及其影响因素,为制订针对性干预措施提供依据。方法2024年9-12月,采用便利抽样法选取湖南、广西两省3所三级甲等医院生殖中心就诊的314例ART助孕女性为研究对象,采用患者报告结局经济毒性综合评分量表(comprehensive scores for financial toxicity based on the patient-reported outcome measure,COST-PROM)、中文版不孕症生育压力简式量表(Chinese version of the COMPI fertility problem stress scale,COMPI-FPSS)、感知伴侣回应性量表(perceived partner responsiveness scale,PPRS)、心理弹性量表(Connor-Davidson resilience scale,CD-RISC)对其进行调查。结果ART助孕女性COST-PROM得分为(15.72±7.55)分,其中92.04%(289例)存在经济毒性。多元线性回归分析显示,门诊报销比例、家庭储蓄、助孕次数、异地医疗、ART相关并发症、生育压力、心理弹性是经济毒性的影响因素(均P<0.01),解释总变异的41.74%。结论ART助孕女性普遍存在经济毒性,医护人员应早期识别高危人群,并根据影响因素制订针对性生育护理策略,以降低该人群的经济毒性水平。展开更多
Leaves and glumes act as lateral organs and have essential effects on photosynthesis and seed morphology,thus affecting yield.However,the molecular mechanisms controlling their polarity development in rice still need ...Leaves and glumes act as lateral organs and have essential effects on photosynthesis and seed morphology,thus affecting yield.However,the molecular mechanisms controlling their polarity development in rice still need further study.Here,we isolated a polarity defect of lateral organs 1(pdl1)mutant in rice,which exhibits twisted/filamentous-shaped leaves and cracked/filamentous-shaped lemmas caused by defects in polarity development.PDL1 encodes a SUPPRESSOR OF GENE SILENCING 3 protein localized in the cytoplasmic granules.PDL1 is expressed in the shoot apical meristem,inflorescence meristem,floral meristem,and lateral organs including leaves and floral organs.PDL1 is involved in the synthesis of tasiR-ARF,which may subsequently modulate the expression of OsARFs.Meanwhile,the expression levels of abaxial miR165/166 and the adaxial identity genes OSHBs were respectively increased and reduced significantly.The results of this study clarify the molecular mechanism by which PDL1-mediated tasiR-ARF synthesis regulates the lateral organ polarity development in rice.展开更多
Salinity is a major environmental stress affecting crop growth and productivity globally.The application of halotolerant plant growth-promoting bacteria(HT-PGPB)has been widely recognized to promote crop growth and re...Salinity is a major environmental stress affecting crop growth and productivity globally.The application of halotolerant plant growth-promoting bacteria(HT-PGPB)has been widely recognized to promote crop growth and reduce the adverse effects of salt stress.In this study,key endophytic bacteria that can respond to salinity changes were identified by analyzing the microbial community in propagules of Kandelia obovata.Delftia tsuruhatensis DYX29,a strain that can grow normally under high salinity conditions with a sodium chloride(NaCl)concentration of 5%(w/v),was obtained by pure culture.DYX29 can produce siderophores with a siderophore unit value of 87.6%and 1-aminocyclopropane-1-carboxylate(ACC)deaminase with 29 UL^(-1),and its synthesis of intracellular amino acids and auxin can be induced by high salinity.Inoculation with DYX29 can remarkably promote the salt tolerance of rice.Under salt stress,the addition of DYX29 was shown to effectively promote the growth of rice seedlings through a variety of approaches.It increased the biomass of rice seedlings by 32.9%(dry weight)and promoted the accumulation of soluble sugars by 23.1%.It also increased catalase(CAT)and peroxidase(POD)activities in rice leaves by 37.8 and 88.2%,respectively.Moreover,it maintained the ionic homeostasis in rice roots and leaves.In addition,it upregulated the expression of growth-promoting hormones,such as indole-3-acetic acid(IAA),brassinolide(BL),abscisic acid(ABA),and salicylic acid(SA),in rice roots by 27.8,69.5,123.7 and 28.6%,respectively.This study provides inspiration for screening valuable salt-tolerant pro-biotic bacteria from mangrove ecosystems and their use for crop growth promotion under salt stress.It can also provide useful references for the development of new salt-tolerant and pro-biotic biofertilizers,as well as the investigation of the related mechanisms.展开更多
基金supported by the National Natural Science Foundation of China(32270296)the Shenzhen Postdoctoral Scientific Research,China(77000-42100004)+1 种基金the Key Basic Research and Development Program of Hubei Province,China(2020BBA052)the Natural Science Foundation of Guangdong Province,China(2024A1515010498)and the Fundamental Research Funds for the Central Universities,Sun Yat-sen University,China.
文摘Sharp eyespot(Rhizoctonia cerealis)is a widespread soil-borne fungal disease that poses a severe threat to wheat health,and it is one of the main obstacles to achieving stable and high-quality wheat yields in China.Our collaborative team has developed a novel,efficient,and low-toxicity fungicide named Y17991(N-(2-(2,4-bis-(trifluoromethyl)phenoxy)phenyl)-3-(difluoromethyl)-1-methyl-1H-pyrazole-4-carboxamide).Preliminary laboratory tests confirmed the significant inhibitory effect of this agent on R.cerealis.Large-area field trials also demonstrated its efficacy,with a disease prevention index of 83.52%,which is 1.97%greater than that of the widely used thifluzamide,and it significantly increased the wheat yield.Moreover,this study explored the impacts of Y17991 on the structure and function of the microbial community in wheat rhizosphere soil.Bacterial communities were more strongly affected than fungal communities.Y17991 significantly modulated key amino acid metabolic pathways and certain biosynthetic processes in diseased wheat rhizospheres,and it also enhanced certain biosynthetic pathways and metabolic activities in healthy wheat rhizospheres.Additionally,the application of Y17991 regulated rhizosphere metabolites,thus exerting significant control over the microbial community.We identified 15 microbial strains potentially involved in the prevention and treatment of R.cerealis,and Y17991 treatment promoted the growth of Pedobacter and Bacillus strains.These strains not only aid in plant growth but they also have the potential for disease prevention.In summary,Y17991 application at a reasonable dose does not cause significant disruption to nontarget rhizosphere microbial communities.In future studies,we will continue to investigate the impacts of Y17991 on nonmicrobial components in soil ecosystems,such as protozoa and nematodes.Our research provides a theoretical basis for the scientific application and promotion of new fungicides and offers a significant reference for establishing a comprehensive system for assessing the ecological impact of pesticides on the environment.
基金supported by the National Undergraduate Innovation and Entrepreneurship Training Program(No.201910060034)the Science and Technology Development Fund Program of Universities of Tianjin(No.2017KJ250)+2 种基金the Key Program of Tianjin Science and Technology Development Plan(No.18YFYSZC00180)the Tianjin Natural Science Foundation(Nos.18JCQNJC72700 and 18JCZDJC99800)the Tianjin Science and Technology Foundation(No.17ZXZNGX00090).
文摘Gallium oxide(Ga2O3) thin films were prepared on Si substrate by magnetron sputtering. The obtained samples were comprehensively characterized by X-ray photoelectron spectroscopy(XPS) and scanning electron microscope(SEM). Ti, Pt, Ni and AZO were deposited on the Ga2O3 thin films as electrodes. This paper mainly studies the metal-semiconductor contact formed by these four materials on the films and the influence of annealing at 500℃ on the metal-semiconductor contact. The I-V characteristics show a good linear relationship, which indicates ohmic contact between Ga2O3 and other electrodes.
文摘Objective: To investigate the influence of hyperbaric oxygen combined with psychotropic drugs on serum neurotransmitter and cytokine contents in patients with post-stroke depression. Methods: A total of 62 patients with post-stroke depression who were treated in the hospital between January 2015 and February 2017 were divided into routine group (n=31) and hyperbaric oxygen group (n=31) by random number table method. Routine group received antidepressant therapy, hyperbaric oxygen group received hyperbaric oxygen combined with antidepressant therapy, and the therapies lasted for 8 weeks. The differences in the serum contents of monoamine neurotransmitters, inflammatory factors and oxidative stress indexes were compared between the two groups before and after treatment. Results: There was no statistically significant difference in serum levels of monoamine neurotransmitters, inflammatory factors and oxidative stress indexes between the two groups before treatment. After 8 weeks of treatment, serum monoamine neurotransmitters NE, 5-HT and DA contents of hyperbaric oxygen group were higher than those of routine group;serum inflammatory factors IL-1, IL-6, IL-8 and hs-CRP contents were lower than those of routine group;serum oxidative stress indexes LHP and MDA contents were lower than those of routine group whereas CAT and GSH-Px contents were higher than those of routine group. Conclusion:Hyperbaric oxygen combined with psychotropic drug therapy can effectively increase the expression of monoamine neurotransmitters and balance the homeostasis in patients with post-stroke depression.
文摘目的探讨辅助生殖技术(assisted reproductive technology,ART)助孕女性经济毒性的现状及其影响因素,为制订针对性干预措施提供依据。方法2024年9-12月,采用便利抽样法选取湖南、广西两省3所三级甲等医院生殖中心就诊的314例ART助孕女性为研究对象,采用患者报告结局经济毒性综合评分量表(comprehensive scores for financial toxicity based on the patient-reported outcome measure,COST-PROM)、中文版不孕症生育压力简式量表(Chinese version of the COMPI fertility problem stress scale,COMPI-FPSS)、感知伴侣回应性量表(perceived partner responsiveness scale,PPRS)、心理弹性量表(Connor-Davidson resilience scale,CD-RISC)对其进行调查。结果ART助孕女性COST-PROM得分为(15.72±7.55)分,其中92.04%(289例)存在经济毒性。多元线性回归分析显示,门诊报销比例、家庭储蓄、助孕次数、异地医疗、ART相关并发症、生育压力、心理弹性是经济毒性的影响因素(均P<0.01),解释总变异的41.74%。结论ART助孕女性普遍存在经济毒性,医护人员应早期识别高危人群,并根据影响因素制订针对性生育护理策略,以降低该人群的经济毒性水平。
基金supported by the National Natural Science Foundation of China(32470354,31900612,and 31971919)the Natural Science Foundation of Chongqing,China(cstc2020jcyj-jqX0020)+1 种基金the Foundation for Innovative Research Groups of the Natural Science Foundation of Chongqing,China(cstc2021jcyj-cxttX0004)the Chongqing Talent Program Foundation,China(cstc2024ycjhbgzxm0063)。
文摘Leaves and glumes act as lateral organs and have essential effects on photosynthesis and seed morphology,thus affecting yield.However,the molecular mechanisms controlling their polarity development in rice still need further study.Here,we isolated a polarity defect of lateral organs 1(pdl1)mutant in rice,which exhibits twisted/filamentous-shaped leaves and cracked/filamentous-shaped lemmas caused by defects in polarity development.PDL1 encodes a SUPPRESSOR OF GENE SILENCING 3 protein localized in the cytoplasmic granules.PDL1 is expressed in the shoot apical meristem,inflorescence meristem,floral meristem,and lateral organs including leaves and floral organs.PDL1 is involved in the synthesis of tasiR-ARF,which may subsequently modulate the expression of OsARFs.Meanwhile,the expression levels of abaxial miR165/166 and the adaxial identity genes OSHBs were respectively increased and reduced significantly.The results of this study clarify the molecular mechanism by which PDL1-mediated tasiR-ARF synthesis regulates the lateral organ polarity development in rice.
基金supported by funds from the National Natural Science Foundation of China(32270296)the Natural Science Foundation of Guangdong Province,China(2024A1515010498)+1 种基金the Shenzhen Postdoctoral Scientific Research,China(77000-42100004)the Fundamental Research Funds for the Central Universities,Sun Yat-sen University,China。
文摘Salinity is a major environmental stress affecting crop growth and productivity globally.The application of halotolerant plant growth-promoting bacteria(HT-PGPB)has been widely recognized to promote crop growth and reduce the adverse effects of salt stress.In this study,key endophytic bacteria that can respond to salinity changes were identified by analyzing the microbial community in propagules of Kandelia obovata.Delftia tsuruhatensis DYX29,a strain that can grow normally under high salinity conditions with a sodium chloride(NaCl)concentration of 5%(w/v),was obtained by pure culture.DYX29 can produce siderophores with a siderophore unit value of 87.6%and 1-aminocyclopropane-1-carboxylate(ACC)deaminase with 29 UL^(-1),and its synthesis of intracellular amino acids and auxin can be induced by high salinity.Inoculation with DYX29 can remarkably promote the salt tolerance of rice.Under salt stress,the addition of DYX29 was shown to effectively promote the growth of rice seedlings through a variety of approaches.It increased the biomass of rice seedlings by 32.9%(dry weight)and promoted the accumulation of soluble sugars by 23.1%.It also increased catalase(CAT)and peroxidase(POD)activities in rice leaves by 37.8 and 88.2%,respectively.Moreover,it maintained the ionic homeostasis in rice roots and leaves.In addition,it upregulated the expression of growth-promoting hormones,such as indole-3-acetic acid(IAA),brassinolide(BL),abscisic acid(ABA),and salicylic acid(SA),in rice roots by 27.8,69.5,123.7 and 28.6%,respectively.This study provides inspiration for screening valuable salt-tolerant pro-biotic bacteria from mangrove ecosystems and their use for crop growth promotion under salt stress.It can also provide useful references for the development of new salt-tolerant and pro-biotic biofertilizers,as well as the investigation of the related mechanisms.
