Wound healing progresses through many key cellular activities,including fibroblast and keratinocyte prolifer-ation and angiogenesis.This study explored the wound-healing potential of reactive oxygen species(ROS)-gener...Wound healing progresses through many key cellular activities,including fibroblast and keratinocyte prolifer-ation and angiogenesis.This study explored the wound-healing potential of reactive oxygen species(ROS)-generating hyaluronic acid(HA)hydrogels.We fabricated a chlorin e6-conjugated HA(Ce6-HA)hydrogel that generates ROS when subjected to irradiation from an LED light source.In vitro studies revealed that the ROS generated by the Ce6-HA hydrogels enhanced the proliferation of fibroblasts and keratinocytes.Further,the fibroblasts were found to have high levels of intracellular ROS,elevated expression of p-ERK1/2,p-p38 MAPK,p-Akt,and cyclin D1 proteins,and enhanced collagen deposition.Moreover,the Ce6-HA hydrogel also promoted endothelial angiogenesis in vitro.In vivo studies demonstrated the ROS-generating HA hydrogels significantly improved wound closure and tissue regeneration compared to control groups.The Ce6-HA hydrogel-treated group exhibited accelerated wound healing,with enhanced fibroblast proliferation,increased keratinocyte proliferation,and better angiogenesis.Histopathological and immunohistochemical analyses showed elevated levels of key growth factors and signaling molecules,which are critical to wound healing.The controlled ROS generation from the Ce6-HA hydrogels activated broader molecular pathways necessary for effective skin tissue repair.Therefore,ROS-triggering HA hydrogels could be a viable approach to accelerate recovery and reduce scarring in clinical settings.展开更多
As we age,our skin loses elasticity and wrinkles form.To prevent this,most people try to improve skin wrinkles by performing procedures such as fillers,and absorbable lifting threads.Another way to solve this structur...As we age,our skin loses elasticity and wrinkles form.To prevent this,most people try to improve skin wrinkles by performing procedures such as fillers,and absorbable lifting threads.Another way to solve this structural problem is to use an elastic thread.Although elastic sutures made of polymer materials(such as silicone)are widely used,data regarding their properties and potential effectiveness are lacking.This study aimed to investigate the effects of inserting non-absorbable elastic threads,with different manufacturing requirements and methods,on the skin and subcutaneous tissue.In this study,non-absorbable elastic threads ELATENS and Elasticum using different manufacturing methods were compared.Each thread was transplanted into the subcutaneous layer of the back of a rat to induce wrinkles.After inducing wrinkles in the skin of rat,the degree of skin maintenance by each thread and the thickness of the capsule formed around the suture were measured.Through ex-vivo experiments on each thread,the fixation force in the tissue was confirmed.In a comparison of inflammatory response and collagen formation through histological analysis,it was confirmed that there was no significant difference from the equivalent comparative product.In conclusion,the degree of encapsulation between tissues and collagen fiber formation for improving skin wrinkles was superior in elastic threads compared to non-elastic threads.It is believed that this provides certain elasticity to the skin layer and can induce cell influx to improve wrinkles.展开更多
Decellularization to produce bioscaffolds composed of the extracellular matrix(ECM)uses enzymatic,chemical and physical methods to remove antigens and cellular components from tissues.Effective decellularization metho...Decellularization to produce bioscaffolds composed of the extracellular matrix(ECM)uses enzymatic,chemical and physical methods to remove antigens and cellular components from tissues.Effective decellularization methods depend on the characteristics of tissues,and in particular,tissues with dense,complex structure and abundant lipid content are difficult to completely decellularize.Our study enables future research on the development of methods and treatments for fabricating bioscaffolds via decellularization of complex and rigid skin tissues,which are not commonly considered for decellularization to date as their structural and functional characteristics could not be preserved after severe decellularization.In this study,decellularization of human dermal tissue was done by a combination of both chemical(0.05%trypsin-EDTA,2%SDS and 1%Triton X-100)and physical methods(electroporation and sonication).After decellularization,the content of DNA remaining in the tissue was quantitatively confirmed,and the structural change of the tissue and the retention and distribution of ECM components were evaluated through histological and histochemical analysis,respectively.Conditions of the chemical pretreatment that increase the efficiency of physical stimulation as well as decellularization,and conditions for electroporation and sonication without the use of detergents,unlike the methods performed in previous studies,were established to enable the complete decellularization of the skin tissue.The combinatorial decellularization treatment formed micropores in the lipid bilayers of the skin tissues while removing all cell and cellular residues without affecting the ECM properties.Therefore,this procedure can be widely used to fabricate bioscaffolds by decellularizing biological tissues with dense and complex structures.展开更多
Reactive oxygen species(ROS)are byproducts of cellular metabolism;they play a significant role as secondary messengers in cell signaling.In cells,high concentrations of ROS induce apoptosis,senescence,and contact inhi...Reactive oxygen species(ROS)are byproducts of cellular metabolism;they play a significant role as secondary messengers in cell signaling.In cells,high concentrations of ROS induce apoptosis,senescence,and contact inhibition,while low concentrations of ROS result in angiogenesis,proliferation,and cytoskeleton remodeling.Thus,controlling ROS generation is an important factor in cell biology.We designed a chlorin e6(Ce6)-immobilized polyethylene terephthalate(PET)film(Ce6-PET)to produce extracellular ROS under red-light irradiation.The application of Ce6-PET films can regulate the generation of ROS by altering the intensity of light-emitting diode sources.We confirmed that the Ce6-PET film could effectively promote cell growth under irradiation at 500 μW/cm^(2) for 30 min in human umbilical vein endothelial cells.We also found that the Ce6-PET film is more efficient in generating ROS than a Ce6-incorporated polyurethane film under the same conditions.Ce6-PET fabrication shows promise for improving the localized delivery of extracellular ROS and regulating ROS formation through the optimization of irradiation intensity.展开更多
基金supported by the Korean Fund for Regenerative Medicine(KFRM)grant funded by the Korea government(the Ministry of Science and ICT,the Ministry of Health&Welfare)(code:KFRM RS-2023-00215084).
文摘Wound healing progresses through many key cellular activities,including fibroblast and keratinocyte prolifer-ation and angiogenesis.This study explored the wound-healing potential of reactive oxygen species(ROS)-generating hyaluronic acid(HA)hydrogels.We fabricated a chlorin e6-conjugated HA(Ce6-HA)hydrogel that generates ROS when subjected to irradiation from an LED light source.In vitro studies revealed that the ROS generated by the Ce6-HA hydrogels enhanced the proliferation of fibroblasts and keratinocytes.Further,the fibroblasts were found to have high levels of intracellular ROS,elevated expression of p-ERK1/2,p-p38 MAPK,p-Akt,and cyclin D1 proteins,and enhanced collagen deposition.Moreover,the Ce6-HA hydrogel also promoted endothelial angiogenesis in vitro.In vivo studies demonstrated the ROS-generating HA hydrogels significantly improved wound closure and tissue regeneration compared to control groups.The Ce6-HA hydrogel-treated group exhibited accelerated wound healing,with enhanced fibroblast proliferation,increased keratinocyte proliferation,and better angiogenesis.Histopathological and immunohistochemical analyses showed elevated levels of key growth factors and signaling molecules,which are critical to wound healing.The controlled ROS generation from the Ce6-HA hydrogels activated broader molecular pathways necessary for effective skin tissue repair.Therefore,ROS-triggering HA hydrogels could be a viable approach to accelerate recovery and reduce scarring in clinical settings.
文摘As we age,our skin loses elasticity and wrinkles form.To prevent this,most people try to improve skin wrinkles by performing procedures such as fillers,and absorbable lifting threads.Another way to solve this structural problem is to use an elastic thread.Although elastic sutures made of polymer materials(such as silicone)are widely used,data regarding their properties and potential effectiveness are lacking.This study aimed to investigate the effects of inserting non-absorbable elastic threads,with different manufacturing requirements and methods,on the skin and subcutaneous tissue.In this study,non-absorbable elastic threads ELATENS and Elasticum using different manufacturing methods were compared.Each thread was transplanted into the subcutaneous layer of the back of a rat to induce wrinkles.After inducing wrinkles in the skin of rat,the degree of skin maintenance by each thread and the thickness of the capsule formed around the suture were measured.Through ex-vivo experiments on each thread,the fixation force in the tissue was confirmed.In a comparison of inflammatory response and collagen formation through histological analysis,it was confirmed that there was no significant difference from the equivalent comparative product.In conclusion,the degree of encapsulation between tissues and collagen fiber formation for improving skin wrinkles was superior in elastic threads compared to non-elastic threads.It is believed that this provides certain elasticity to the skin layer and can induce cell influx to improve wrinkles.
基金supported by the National Research Foundation of Korea(NRF)grant funded by the Korea government(MSIT)(No.2017M3A9B3063638,No.2019R1A2C2005256).
文摘Decellularization to produce bioscaffolds composed of the extracellular matrix(ECM)uses enzymatic,chemical and physical methods to remove antigens and cellular components from tissues.Effective decellularization methods depend on the characteristics of tissues,and in particular,tissues with dense,complex structure and abundant lipid content are difficult to completely decellularize.Our study enables future research on the development of methods and treatments for fabricating bioscaffolds via decellularization of complex and rigid skin tissues,which are not commonly considered for decellularization to date as their structural and functional characteristics could not be preserved after severe decellularization.In this study,decellularization of human dermal tissue was done by a combination of both chemical(0.05%trypsin-EDTA,2%SDS and 1%Triton X-100)and physical methods(electroporation and sonication).After decellularization,the content of DNA remaining in the tissue was quantitatively confirmed,and the structural change of the tissue and the retention and distribution of ECM components were evaluated through histological and histochemical analysis,respectively.Conditions of the chemical pretreatment that increase the efficiency of physical stimulation as well as decellularization,and conditions for electroporation and sonication without the use of detergents,unlike the methods performed in previous studies,were established to enable the complete decellularization of the skin tissue.The combinatorial decellularization treatment formed micropores in the lipid bilayers of the skin tissues while removing all cell and cellular residues without affecting the ECM properties.Therefore,this procedure can be widely used to fabricate bioscaffolds by decellularizing biological tissues with dense and complex structures.
基金This work was supported by a National Research Foundation of Korea(NRF)grant funded by the Korean government(MSIT,Nos 2017M3A9B3063638 and 2019R1A2C2005256).
文摘Reactive oxygen species(ROS)are byproducts of cellular metabolism;they play a significant role as secondary messengers in cell signaling.In cells,high concentrations of ROS induce apoptosis,senescence,and contact inhibition,while low concentrations of ROS result in angiogenesis,proliferation,and cytoskeleton remodeling.Thus,controlling ROS generation is an important factor in cell biology.We designed a chlorin e6(Ce6)-immobilized polyethylene terephthalate(PET)film(Ce6-PET)to produce extracellular ROS under red-light irradiation.The application of Ce6-PET films can regulate the generation of ROS by altering the intensity of light-emitting diode sources.We confirmed that the Ce6-PET film could effectively promote cell growth under irradiation at 500 μW/cm^(2) for 30 min in human umbilical vein endothelial cells.We also found that the Ce6-PET film is more efficient in generating ROS than a Ce6-incorporated polyurethane film under the same conditions.Ce6-PET fabrication shows promise for improving the localized delivery of extracellular ROS and regulating ROS formation through the optimization of irradiation intensity.
