Polyetheretherketone(PEEK)is a desirable candidate to replace conventional metal implants owing to its excellent mechanical properties.However,the intrinsic bioinertness of PEEK results in inferior or delayed osseoint...Polyetheretherketone(PEEK)is a desirable candidate to replace conventional metal implants owing to its excellent mechanical properties.However,the intrinsic bioinertness of PEEK results in inferior or delayed osseointegration,which limits its further clinical application.To address these challenges,one leading strategy is to construct a biofunctionalized surface on PEEK that provides a coordinated osteoblastosteoclast interactions microenvironment.Herein,alendronate(ALN),a common bone absorption inhibitor,was loaded in biomedical inorganic/organic microspheres,consisting of bioactive inorganic nanohydroxyapatite core,and chitosan(CS)shell.Polydopamine(PDA)modification was employed to ensure the adherence of the microspheres to the PEEK surface.The delivery of ALN and Ca^(2+)from these microspheres simultaneously suppressed osteoclastogenesis and promoted osteogenesis,resulting in a coordinated cascade of osteoblast-osteoclast interactions crucial for the per-implant osseointegration.In vitro experiments demonstrated that the PEEK surface exhibited satisfactory biocompatibility and enhanced the proliferation and osteogenic differentiation of rat bone mesenchymal stem cells while inhibiting the osteoclast differentiation.Moreover,the in vivo rat femoral drilling model demonstrated superior osseointegration three months after implantation.By considering the bone remodeling processes,this study proposes a novel biofunctionalized PEEK surface that regulates the activities of both osteoblasts and osteoclasts to promote osseointegration.展开更多
基金the funding support from the Tianjin Medical University“Clinical Talent Training 123 Climbing Plan”the Youth Fund of Tianjin Medical University Second Hospital(No.2022ydey06)。
文摘Polyetheretherketone(PEEK)is a desirable candidate to replace conventional metal implants owing to its excellent mechanical properties.However,the intrinsic bioinertness of PEEK results in inferior or delayed osseointegration,which limits its further clinical application.To address these challenges,one leading strategy is to construct a biofunctionalized surface on PEEK that provides a coordinated osteoblastosteoclast interactions microenvironment.Herein,alendronate(ALN),a common bone absorption inhibitor,was loaded in biomedical inorganic/organic microspheres,consisting of bioactive inorganic nanohydroxyapatite core,and chitosan(CS)shell.Polydopamine(PDA)modification was employed to ensure the adherence of the microspheres to the PEEK surface.The delivery of ALN and Ca^(2+)from these microspheres simultaneously suppressed osteoclastogenesis and promoted osteogenesis,resulting in a coordinated cascade of osteoblast-osteoclast interactions crucial for the per-implant osseointegration.In vitro experiments demonstrated that the PEEK surface exhibited satisfactory biocompatibility and enhanced the proliferation and osteogenic differentiation of rat bone mesenchymal stem cells while inhibiting the osteoclast differentiation.Moreover,the in vivo rat femoral drilling model demonstrated superior osseointegration three months after implantation.By considering the bone remodeling processes,this study proposes a novel biofunctionalized PEEK surface that regulates the activities of both osteoblasts and osteoclasts to promote osseointegration.
