Panax Ginseng(2n=48)represents a quintessential resource in traditional Chinese medicine,renowned for its outstanding medicinal and economic benefits(Choi,2008).But the late start in analyzing the ginseng genome and t...Panax Ginseng(2n=48)represents a quintessential resource in traditional Chinese medicine,renowned for its outstanding medicinal and economic benefits(Choi,2008).But the late start in analyzing the ginseng genome and the poorly developed genetic transformation system still impede the study of ginseng gene function and the application of molecular breeding.Transient transformation has the advantages of high efficiency,low cost,and short cycle while laying the foundation for stable genetic transformation(Chen et al.,2021).In the plant transformation process,the cell wall prevents exogenous DNA or protein entry,significantly reducing the efficiency of the transformation.Protoplasts,as exposed cells wrapped by the plasma membrane,are more likely to absorb exogenous DNA,RNA,and protein.Transgenic systems of protoplasts have been established in several species and applied in many fields,such as gene function research(Gou et al.,2020),gene editing(Yang et al.,2023),and physiological or molecular mechanism research(Aoyagi,2011).For instance,Oryza sativa protoplasts were employed to screen genes involved in rice defense signaling pathways through fluorescent reporter systems,with BiFC employed to verified inter-protein interactions(He et al.,2016).A study transformed Cannabis sativa L.protoplasts with the plasmids carrying GFP and RFP genes,evaluated the efficiency under different transformation conditions by flow cytometry,and verified the induction of synthetic DR5 promoter by IAA based on the constructed system(Beard et al.,2021).展开更多
基金supported by the Genetic analysis of important quality and traits of ginseng and basic research on molecular design breeding(Grant No.U21A20405)。
文摘Panax Ginseng(2n=48)represents a quintessential resource in traditional Chinese medicine,renowned for its outstanding medicinal and economic benefits(Choi,2008).But the late start in analyzing the ginseng genome and the poorly developed genetic transformation system still impede the study of ginseng gene function and the application of molecular breeding.Transient transformation has the advantages of high efficiency,low cost,and short cycle while laying the foundation for stable genetic transformation(Chen et al.,2021).In the plant transformation process,the cell wall prevents exogenous DNA or protein entry,significantly reducing the efficiency of the transformation.Protoplasts,as exposed cells wrapped by the plasma membrane,are more likely to absorb exogenous DNA,RNA,and protein.Transgenic systems of protoplasts have been established in several species and applied in many fields,such as gene function research(Gou et al.,2020),gene editing(Yang et al.,2023),and physiological or molecular mechanism research(Aoyagi,2011).For instance,Oryza sativa protoplasts were employed to screen genes involved in rice defense signaling pathways through fluorescent reporter systems,with BiFC employed to verified inter-protein interactions(He et al.,2016).A study transformed Cannabis sativa L.protoplasts with the plasmids carrying GFP and RFP genes,evaluated the efficiency under different transformation conditions by flow cytometry,and verified the induction of synthetic DR5 promoter by IAA based on the constructed system(Beard et al.,2021).
