Due to the rising global demand for natural food additives and their critical role in public health security,lactic acid bacteria(LAB)have garnered significant attention as natural biocontrol agents.This study utilize...Due to the rising global demand for natural food additives and their critical role in public health security,lactic acid bacteria(LAB)have garnered significant attention as natural biocontrol agents.This study utilized the Oxford cup method to screen LAB strains based on inhibition zone diameters against Salmonella,followed by comprehensive characterization of growth kinetics and probiotic potential.The results demonstrated that the selected strains exhibited gamma-hemolytic activity(non-pathogenic phenotype),susceptibility to seven clinically relevant antibiotics,robust environmental resilience(>85% survival under simulated gastrointestinal stress),and potent antimicrobial efficacy.Notably,Pediococcus acidilactici OE-9 cell-free supernatant(CFS)showed exceptional performance,eliminating Salmonella within 6 h at 28℃,with antibacterial effects persisting for 7 days,while also inhibiting biofilm formation on common food-contact surfaces in high-level contamination scenarios(10^(6) CFU/mL Salmonella in eggshells),treatment with OE-9 CFS resulted in a 3-log reduction in pathogen viability.Based on these findings,the P.acidilactici OE-9 strain emerges as a novel LAB strain with remarkable antibacterial and bacteriostatic properties,holding significant potential for application in food preservation.展开更多
基金supported by National Key Research and Development Program of China(2023YFF1104100&2023YFD1801000)Guangdong Major Project of Basic and Applied Basic Research(2020B0301030005)the Talent Support Project of Guangdong(2021TQ06N119).
文摘Due to the rising global demand for natural food additives and their critical role in public health security,lactic acid bacteria(LAB)have garnered significant attention as natural biocontrol agents.This study utilized the Oxford cup method to screen LAB strains based on inhibition zone diameters against Salmonella,followed by comprehensive characterization of growth kinetics and probiotic potential.The results demonstrated that the selected strains exhibited gamma-hemolytic activity(non-pathogenic phenotype),susceptibility to seven clinically relevant antibiotics,robust environmental resilience(>85% survival under simulated gastrointestinal stress),and potent antimicrobial efficacy.Notably,Pediococcus acidilactici OE-9 cell-free supernatant(CFS)showed exceptional performance,eliminating Salmonella within 6 h at 28℃,with antibacterial effects persisting for 7 days,while also inhibiting biofilm formation on common food-contact surfaces in high-level contamination scenarios(10^(6) CFU/mL Salmonella in eggshells),treatment with OE-9 CFS resulted in a 3-log reduction in pathogen viability.Based on these findings,the P.acidilactici OE-9 strain emerges as a novel LAB strain with remarkable antibacterial and bacteriostatic properties,holding significant potential for application in food preservation.
