[Objectives]This study was conducted to obtain the best planting technology system of Dysosma versipellis.[Methods]The rhizomes of D.versipellis were selected as the propagation material.Experiments were conducted on ...[Objectives]This study was conducted to obtain the best planting technology system of Dysosma versipellis.[Methods]The rhizomes of D.versipellis were selected as the propagation material.Experiments were conducted on one-year-old,two-year-old and three-year-old rhizomes to investigate the effects of hormone formula,soaking time,and growth years on the planting technology.[Results]The effects of various factors on the growth rate of D.versipellis ranked as hormone formula>rhizome age>soaking time(h).The optimal combination was A_(2)B_(2)C_(3),which corresponded to three-year-old rhizomes soaked for 12 h in a hormone solution containing BA 2 mg/L+NAA 0.2 mg/L.Adventitious roots of D.versipellis(including isolated adventitious roots and adventitious roots attached to two-year-old rhizomes)were also used for propagation,while also considering whether to add Shuangjier(GGR)solution,with soaking times in the GGR solution set at 4,12,and 24 h,respectively.The results indicated that soaking the roots attached to rhizomes in GGR solution for 12 h yielded the highest growth rate.[Conclusions]This study not only provides technical support for the cultivation of D.versipellis,but also offers a reference for formulating corresponding Technical Operating Procedures(SOP)and establishing demonstration bases.展开更多
基金Supported by National Undergraduate Innovation and Entrepreneurship Training Program of Faculty of Chinese Medicine Science of Guangxi University of Chinese Medicine(202113643016)Univeristy-level Scientific Research Project of Faculty of Chinese Medicine Science of Guangxi University of Chinese Medicine in 2022(2022MS017)National Key Research and Development Program of China(2019YFC171230).
文摘[Objectives]This study was conducted to obtain the best planting technology system of Dysosma versipellis.[Methods]The rhizomes of D.versipellis were selected as the propagation material.Experiments were conducted on one-year-old,two-year-old and three-year-old rhizomes to investigate the effects of hormone formula,soaking time,and growth years on the planting technology.[Results]The effects of various factors on the growth rate of D.versipellis ranked as hormone formula>rhizome age>soaking time(h).The optimal combination was A_(2)B_(2)C_(3),which corresponded to three-year-old rhizomes soaked for 12 h in a hormone solution containing BA 2 mg/L+NAA 0.2 mg/L.Adventitious roots of D.versipellis(including isolated adventitious roots and adventitious roots attached to two-year-old rhizomes)were also used for propagation,while also considering whether to add Shuangjier(GGR)solution,with soaking times in the GGR solution set at 4,12,and 24 h,respectively.The results indicated that soaking the roots attached to rhizomes in GGR solution for 12 h yielded the highest growth rate.[Conclusions]This study not only provides technical support for the cultivation of D.versipellis,but also offers a reference for formulating corresponding Technical Operating Procedures(SOP)and establishing demonstration bases.