In order to better the structure and immunomodulatory activity relationships of Dendrobium officinale polysaccharides,this study partially degraded DOP with a molecular weight of 264 kDa using endo-β-1,4-mannanase.De...In order to better the structure and immunomodulatory activity relationships of Dendrobium officinale polysaccharides,this study partially degraded DOP with a molecular weight of 264 kDa using endo-β-1,4-mannanase.Dendrobium offinale polysaccharides(DOP)and two degraded fractions(DOP-30:51 kDa and DOP-60:28 kDa)were structurally characterized and evaluated for in vitro immunomodulatory activity.Structural analysis indicated that enzymatic hydrolysis of DOP can effectively produce low molecular weight polysaccharides with similar structures but slightly different chemical compositions.The backbone structures of DOPs were all consisted of→4)-β-D-Manp-(1→,→4)-β-D-Glcp-(1→,→4)-2-O-acetyl-β-D-Manp-(1→and→4)-3-O-acetyl-β-D-Manp-(1→.All DOPs enhanced the proliferation and phagocytosis of RAW264.7 macrophages,promoted the secretion of immune effector molecules,and facilitated the polarized of macrophages to the M1 phenotype,as well as the expression of Toll-like receptor 4(TLR4)in vitro.Among them,DOP-60 showed relatively strong immunomodulatory activity.Correlation analysis further indicated that molecular weight(Mw)was most closely correlated with macrophage activation by DOP,suggesting that low molecular weight DOP has a greater potential to activate macrophages.展开更多
基金supported by the Youth Fund of the National Natural Science Foundation of China(32302014)Program for National Key R&D Program of China(2022YFF1100202)+2 种基金GanPoJunCai Young Technology Talent Support Program of Jiangxi Province(2025QT01)General Program of Natural Science Foundation of Jiangxi Province(20224BAB205040)Key Laboratory of Bioactive Polysaccharides of Jiangxi Province(20212BCD42016).
文摘In order to better the structure and immunomodulatory activity relationships of Dendrobium officinale polysaccharides,this study partially degraded DOP with a molecular weight of 264 kDa using endo-β-1,4-mannanase.Dendrobium offinale polysaccharides(DOP)and two degraded fractions(DOP-30:51 kDa and DOP-60:28 kDa)were structurally characterized and evaluated for in vitro immunomodulatory activity.Structural analysis indicated that enzymatic hydrolysis of DOP can effectively produce low molecular weight polysaccharides with similar structures but slightly different chemical compositions.The backbone structures of DOPs were all consisted of→4)-β-D-Manp-(1→,→4)-β-D-Glcp-(1→,→4)-2-O-acetyl-β-D-Manp-(1→and→4)-3-O-acetyl-β-D-Manp-(1→.All DOPs enhanced the proliferation and phagocytosis of RAW264.7 macrophages,promoted the secretion of immune effector molecules,and facilitated the polarized of macrophages to the M1 phenotype,as well as the expression of Toll-like receptor 4(TLR4)in vitro.Among them,DOP-60 showed relatively strong immunomodulatory activity.Correlation analysis further indicated that molecular weight(Mw)was most closely correlated with macrophage activation by DOP,suggesting that low molecular weight DOP has a greater potential to activate macrophages.
