Oligodontia is the congenital absence of six or more teeth and comprises the more severe forms of tooth agenesis.Many genes have been implicated in the etiology of tooth agenesis,which is highly variable in its clinic...Oligodontia is the congenital absence of six or more teeth and comprises the more severe forms of tooth agenesis.Many genes have been implicated in the etiology of tooth agenesis,which is highly variable in its clinical presentation.The purpose of this study was to identify associations between genetic mutations and clinical features of oligodontia patients.An online systematic search of papers published from January 1992 to June 2021 identified 381 oligodontia cases meeting the eligibility criteria of causative gene mutation,phenotype description,and radiographic records.Additionally,ten families with oligodontia were recruited and their genetic etiologies were determined by whole-exome sequence analyses.We identified a novel mutation in WNT10A(c.99_105dup)and eight previously reported mutations in WNT10A(c.433 G>A;c.682 T>A;c.318 C>G;c.511.C>T;c.321 C>A),EDAR(c.581 C>T),and LRP6(c.1003 C>T,c.2747 G>T).Collectively,20 different causative genes were implicated among those 393 cases with oligodontia.For each causative gene,the mean number of missing teeth per case and the frequency of teeth missing at each position were calculated.Genotype–phenotype correlation analysis indicated that molars agenesis is more likely linked to PAX9 mutations,mandibular first premolar agenesis is least associated with PAX9 mutations.展开更多
The society’s urgent demand for environmentally friendly, safe and low-cost energy storage devices has promoted the research of aqueous zinc-ion batteries. However, the uneven deposition of Zn ions on anodes will lea...The society’s urgent demand for environmentally friendly, safe and low-cost energy storage devices has promoted the research of aqueous zinc-ion batteries. However, the uneven deposition of Zn ions on anodes will lead to the growth of the dendrite and reduce the Coulombic efficiency as well as the lifespan of the devices. Herein, we construct an O,N-dual functionalized carbon cloth current collector via a simple hydrothermal strategy, in which the oxygen-containing functional groups and the N heteroatoms can regulate the transmission and deposition of Zn ions, respectively. The proposed synergistic strategy ensures the uniform distribution of Zn ions on the surface of the Zn anode and inhibits the formation of dendrites. The symmetric cell based on the O,N-dual doped carbon cloth presents superior cycling stability(318 h) with a low voltage hysteresis(11.2 mV) at an areal capacity of 1 m Ah cm^(-2)(20% depth of diacharge). Meanwhile, the appreciably low overpotential(16 m V) and high Columbic efficiency(98.2%)also demonstrate that the O,N-dual functionalized carbon cloth can be worked as a promising host for Zn ions deposition.展开更多
By introducing piezoelectric materials into hydrogel oral dressings,a microelectric field could be generated under stress stimulation,thus facilitating oral wound healing.However,to adapt to the moist and dynamic envi...By introducing piezoelectric materials into hydrogel oral dressings,a microelectric field could be generated under stress stimulation,thus facilitating oral wound healing.However,to adapt to the moist and dynamic environment of the oral cavity,traditional"step-by-step"synthesis often requires the combination of materials with different functionalities.Given the property differences between these materials,this strategy typically involves complex experimental procedures and unnecessary energy consumption.In this study,with the concept of"integrated construction",we innovatively proposed a dual-step photo-induced method and successfully fabricated composite hydrogels with excellent performance.We introduced abundant oxygen vacancies into ZnO,leveraging the enhanced interface dynamics to achieve sustained photo-induced effect.With a double-network polymer framework as a template,this method could achieve the photo-induced spontaneous in-situ synthesis of polydopamine(PDA)within hydrogel without any extra special experimental conditions and complex operation procedures.We conducted a thorough analysis of the mechanism underlying this photo-induced method and applied the as-prepared hydrogel for the treatment of oral wounds,which significantly accelerated the healing process due to the outstanding comprehensive performance of hydrogel.These results suggest novel ideas and theoretical support for the facile construction of high-performance hydrogels based on photodynamic principles,demonstrating immense potential for future applications in wound dressings.展开更多
The indispensability of primary cilia in skeletal development has been widely recognized.We have previously shown that intraflagellar transport 140(IFT140),a protein component of a bidirectional intraflagellar transpo...The indispensability of primary cilia in skeletal development has been widely recognized.We have previously shown that intraflagellar transport 140(IFT140),a protein component of a bidirectional intraflagellar transport system required for ciliary function,controls bone development and dentinogenesis.However,it remains unknown whether IFT140 functionally contributes to bone fracture rehabilitation.Here an osteotomy-induced femoral fracture model was generated in Ift140-transgenic(Ift140-TG)and osteoblast-specific Ift140-conditional knockout(Ift140-cKO)mice.Micro-computed tomography,osteogenic induction,qualitative polymerase chain reaction,and toluidine blue and safranin O/fast green staining assays were used to characterize the dynamics of bone fracture healing from various perspectives.We found that IFT140 was relatively enriched in the bone callus and decreased in fracture-susceptible aged,or diabetic bones.Ift140-cKO mice had impaired osteogenic differentiation from bone mesenchymal stem cells,lower bone mass,and delayed fracture closure,whereas Ift140-TG mice had promising healing outcomes.Overall,our findings demonstrated for the first time that IFT140 has a beneficial role in fracture repair.Future investigation of the primary cilium in the context of aging and osteoporosis would certainly benefit patients at high risk of bone fractures.展开更多
Conventional strategies for highly reversible Zn anodes usually involve complex and time-consuming production processes of current collectors,expensive and toxic electrolyte additives,or the introduction of inactive m...Conventional strategies for highly reversible Zn anodes usually involve complex and time-consuming production processes of current collectors,expensive and toxic electrolyte additives,or the introduction of inactive materials in protective layer.Here,we develop a fast,facile,and environmentally friendly biopolishing method to prepare dendrite-free Zn anodes,which merely involves the simple immersion of Zn foil in a biocompatible cysteine aqueous solution.The ravine structure formed by sulfhydryl etching for 30 min not only increases the electroactive area of Zn anode but also regulates the distribution of electric field and Zn ions,ensuring the homogeneous deposition and stripping of Zn ions.The biopolished Zn anode can be operated steadily for 2,000 h with a low voltage hysteresis at a current density of 1 mA·cm^(−2).In addition,Zn anodes with a cycle life of 500 h can be built by soaking for only 5 min,proving the high efficiency of the proposed method.This strategy is generalized to substances with sulfhydryl groups for polishing Zn electrodes with improved performance.The cysteine-polished Zn//activated carbon supercapacitor can stably run for 20,000 cycles without obvious capacity attenuation.The proposed strategy shows potential for producing advanced Zn anodes.展开更多
The significant characteristics of space non-cooperative targets include the uncertainties of dynamic parameters and behaviors.Herein,a hybrid proximity control strategy adapted to the behavior uncertainty of a non-co...The significant characteristics of space non-cooperative targets include the uncertainties of dynamic parameters and behaviors.Herein,a hybrid proximity control strategy adapted to the behavior uncertainty of a non-cooperative target is presented.First,the relative motion dynamics between the chaser and target is established in the geocentric inertial coordinate system and transcribed based on the chaser spacecraft body coordinate system.Subsequently,to facilitate proximity control under uncertain conditions,an extended state observer is designed to estimate and compensate for the total uncertainty in the relative motion dynamics.Finally,an event-triggered sliding mode control law is designed to track the target with behavior uncertainty and realize synchronization.Numerical simulations demonstrate the effectiveness of the proposed proximity control strategy for both tumbling and maneuvering targets.展开更多
结合了三维结构和亲锌物种的集流体构筑策略被认为是构建高稳定锌金属负极的有效方法.然而,高昂的成本和复杂的制备工艺阻碍了其实际应用.本文通过在有均匀Cu^(2+)锚定的碳布集流体(ACC-600@Cu^(2+))上沉积锌,合理设计了一种稳定的三维...结合了三维结构和亲锌物种的集流体构筑策略被认为是构建高稳定锌金属负极的有效方法.然而,高昂的成本和复杂的制备工艺阻碍了其实际应用.本文通过在有均匀Cu^(2+)锚定的碳布集流体(ACC-600@Cu^(2+))上沉积锌,合理设计了一种稳定的三维锌金属复合阳极(Zn@ACC-600@Cu^(2+)).在锌成核过程中,Cu^(2+)原位还原为金属Cu,然后随着锌的进一步沉积,碳布表面逐渐形成均匀的亲锌的Cu-Zn合金界面层.密度泛函理论计算和实验观察表明,Cu-Zn合金界面不仅可以作为锌离子的亲锌沉积点,而且可以提高导电率,使电场和锌离子通量均匀化.因此,ACC-600@Cu^(2+)集流体可以实现高的镀锌/剥离可逆性,并在15.8 mV的极化电压下稳定循环410 h以上.作为概念验证,我们组装的Zn@ACC-600@Cu^(2+)‖MnO_(2)全电池具有良好的电池倍率性能,与原始碳布相比,其比容量显著提高至110 mA h g^(-1).本文提出的原位还原策略为三维锌金属复合负极的设计提供了一种简便且低成本的方法,促进了无枝晶和高稳定锌金属电池的发展.展开更多
基金supported by the National Institute of Dental and Craniofacial Research(DE015846)a National Research Foundation of Korea(NRF)grant funded by the Korean government(MEST)(NRF-2018R1A5A2024418 and NRF-2020R1A2C2100543)。
文摘Oligodontia is the congenital absence of six or more teeth and comprises the more severe forms of tooth agenesis.Many genes have been implicated in the etiology of tooth agenesis,which is highly variable in its clinical presentation.The purpose of this study was to identify associations between genetic mutations and clinical features of oligodontia patients.An online systematic search of papers published from January 1992 to June 2021 identified 381 oligodontia cases meeting the eligibility criteria of causative gene mutation,phenotype description,and radiographic records.Additionally,ten families with oligodontia were recruited and their genetic etiologies were determined by whole-exome sequence analyses.We identified a novel mutation in WNT10A(c.99_105dup)and eight previously reported mutations in WNT10A(c.433 G>A;c.682 T>A;c.318 C>G;c.511.C>T;c.321 C>A),EDAR(c.581 C>T),and LRP6(c.1003 C>T,c.2747 G>T).Collectively,20 different causative genes were implicated among those 393 cases with oligodontia.For each causative gene,the mean number of missing teeth per case and the frequency of teeth missing at each position were calculated.Genotype–phenotype correlation analysis indicated that molars agenesis is more likely linked to PAX9 mutations,mandibular first premolar agenesis is least associated with PAX9 mutations.
基金supported by the National Natural Science Foundation of China (21825106, 92061201)the Program for Innovative Research Team (in Science and Technology) in Universities of Henan Province (19IRTSTHN022)Zhengzhou University。
文摘The society’s urgent demand for environmentally friendly, safe and low-cost energy storage devices has promoted the research of aqueous zinc-ion batteries. However, the uneven deposition of Zn ions on anodes will lead to the growth of the dendrite and reduce the Coulombic efficiency as well as the lifespan of the devices. Herein, we construct an O,N-dual functionalized carbon cloth current collector via a simple hydrothermal strategy, in which the oxygen-containing functional groups and the N heteroatoms can regulate the transmission and deposition of Zn ions, respectively. The proposed synergistic strategy ensures the uniform distribution of Zn ions on the surface of the Zn anode and inhibits the formation of dendrites. The symmetric cell based on the O,N-dual doped carbon cloth presents superior cycling stability(318 h) with a low voltage hysteresis(11.2 mV) at an areal capacity of 1 m Ah cm^(-2)(20% depth of diacharge). Meanwhile, the appreciably low overpotential(16 m V) and high Columbic efficiency(98.2%)also demonstrate that the O,N-dual functionalized carbon cloth can be worked as a promising host for Zn ions deposition.
基金supported by the National Key R&D Program of China(2021YFB2012601)National Natural Science Foundation of China(No.U2230108)+2 种基金Shanghai Science and Technology Innovation Action Medical Innovation Research Project(23Y11910100)Shanghai Stomatological Hospital Institutional Project(Grant No.SSH-2022-02,SHH-2022-YJ-07,SSH-2023-02)Medical Engineering Integration Project of Fudan University.
文摘By introducing piezoelectric materials into hydrogel oral dressings,a microelectric field could be generated under stress stimulation,thus facilitating oral wound healing.However,to adapt to the moist and dynamic environment of the oral cavity,traditional"step-by-step"synthesis often requires the combination of materials with different functionalities.Given the property differences between these materials,this strategy typically involves complex experimental procedures and unnecessary energy consumption.In this study,with the concept of"integrated construction",we innovatively proposed a dual-step photo-induced method and successfully fabricated composite hydrogels with excellent performance.We introduced abundant oxygen vacancies into ZnO,leveraging the enhanced interface dynamics to achieve sustained photo-induced effect.With a double-network polymer framework as a template,this method could achieve the photo-induced spontaneous in-situ synthesis of polydopamine(PDA)within hydrogel without any extra special experimental conditions and complex operation procedures.We conducted a thorough analysis of the mechanism underlying this photo-induced method and applied the as-prepared hydrogel for the treatment of oral wounds,which significantly accelerated the healing process due to the outstanding comprehensive performance of hydrogel.These results suggest novel ideas and theoretical support for the facile construction of high-performance hydrogels based on photodynamic principles,demonstrating immense potential for future applications in wound dressings.
基金supported by grants from National Natural Science Foundation Projects of China(81822012,82061130222,81771043,92049201)Shanghai Academic Leader of Science and Technology Innovation Action Plan(20XD1424000)the Shanghai Experimental Animal Research Project of Science and Technology Innovation Action Plan(8191101676,201409006400).
文摘The indispensability of primary cilia in skeletal development has been widely recognized.We have previously shown that intraflagellar transport 140(IFT140),a protein component of a bidirectional intraflagellar transport system required for ciliary function,controls bone development and dentinogenesis.However,it remains unknown whether IFT140 functionally contributes to bone fracture rehabilitation.Here an osteotomy-induced femoral fracture model was generated in Ift140-transgenic(Ift140-TG)and osteoblast-specific Ift140-conditional knockout(Ift140-cKO)mice.Micro-computed tomography,osteogenic induction,qualitative polymerase chain reaction,and toluidine blue and safranin O/fast green staining assays were used to characterize the dynamics of bone fracture healing from various perspectives.We found that IFT140 was relatively enriched in the bone callus and decreased in fracture-susceptible aged,or diabetic bones.Ift140-cKO mice had impaired osteogenic differentiation from bone mesenchymal stem cells,lower bone mass,and delayed fracture closure,whereas Ift140-TG mice had promising healing outcomes.Overall,our findings demonstrated for the first time that IFT140 has a beneficial role in fracture repair.Future investigation of the primary cilium in the context of aging and osteoporosis would certainly benefit patients at high risk of bone fractures.
基金supported by the National Natural Science Foundation of China(Nos.21825106 and 92061201)the Program for Innovative Research Team(in Science and Technology)in Universities of Henan Province(No.19IRTSTHN022)Zhengzhou University。
文摘Conventional strategies for highly reversible Zn anodes usually involve complex and time-consuming production processes of current collectors,expensive and toxic electrolyte additives,or the introduction of inactive materials in protective layer.Here,we develop a fast,facile,and environmentally friendly biopolishing method to prepare dendrite-free Zn anodes,which merely involves the simple immersion of Zn foil in a biocompatible cysteine aqueous solution.The ravine structure formed by sulfhydryl etching for 30 min not only increases the electroactive area of Zn anode but also regulates the distribution of electric field and Zn ions,ensuring the homogeneous deposition and stripping of Zn ions.The biopolished Zn anode can be operated steadily for 2,000 h with a low voltage hysteresis at a current density of 1 mA·cm^(−2).In addition,Zn anodes with a cycle life of 500 h can be built by soaking for only 5 min,proving the high efficiency of the proposed method.This strategy is generalized to substances with sulfhydryl groups for polishing Zn electrodes with improved performance.The cysteine-polished Zn//activated carbon supercapacitor can stably run for 20,000 cycles without obvious capacity attenuation.The proposed strategy shows potential for producing advanced Zn anodes.
基金This study was supported by the Qian Xuesen Laboratory of Space Technology,CAST(Grant No.GZZKFJJ2020001)the Open Funding of the National Defense Science and Technology Key Laboratory of Space Intelligent Control Technology(Grant No.6142208200304)the Postdoctoral Research Foundation of Sichuan University.
文摘The significant characteristics of space non-cooperative targets include the uncertainties of dynamic parameters and behaviors.Herein,a hybrid proximity control strategy adapted to the behavior uncertainty of a non-cooperative target is presented.First,the relative motion dynamics between the chaser and target is established in the geocentric inertial coordinate system and transcribed based on the chaser spacecraft body coordinate system.Subsequently,to facilitate proximity control under uncertain conditions,an extended state observer is designed to estimate and compensate for the total uncertainty in the relative motion dynamics.Finally,an event-triggered sliding mode control law is designed to track the target with behavior uncertainty and realize synchronization.Numerical simulations demonstrate the effectiveness of the proposed proximity control strategy for both tumbling and maneuvering targets.
基金supported by the National Natural Science Foundation of China(22001236)the Program for Innovative Research Team(in Science and Technology)in Universities of Henan Province(19IRTSTHN022)Zhengzhou University。
文摘结合了三维结构和亲锌物种的集流体构筑策略被认为是构建高稳定锌金属负极的有效方法.然而,高昂的成本和复杂的制备工艺阻碍了其实际应用.本文通过在有均匀Cu^(2+)锚定的碳布集流体(ACC-600@Cu^(2+))上沉积锌,合理设计了一种稳定的三维锌金属复合阳极(Zn@ACC-600@Cu^(2+)).在锌成核过程中,Cu^(2+)原位还原为金属Cu,然后随着锌的进一步沉积,碳布表面逐渐形成均匀的亲锌的Cu-Zn合金界面层.密度泛函理论计算和实验观察表明,Cu-Zn合金界面不仅可以作为锌离子的亲锌沉积点,而且可以提高导电率,使电场和锌离子通量均匀化.因此,ACC-600@Cu^(2+)集流体可以实现高的镀锌/剥离可逆性,并在15.8 mV的极化电压下稳定循环410 h以上.作为概念验证,我们组装的Zn@ACC-600@Cu^(2+)‖MnO_(2)全电池具有良好的电池倍率性能,与原始碳布相比,其比容量显著提高至110 mA h g^(-1).本文提出的原位还原策略为三维锌金属复合负极的设计提供了一种简便且低成本的方法,促进了无枝晶和高稳定锌金属电池的发展.
