DNA-segment copy number variations(DSCNVs),such as deletions and duplications,are important sources of genomic structural variation.However,the types and sizes of DSCNVs,as well as their genome-wide distribution and p...DNA-segment copy number variations(DSCNVs),such as deletions and duplications,are important sources of genomic structural variation.However,the types and sizes of DSCNVs,as well as their genome-wide distribution and potential functions,are poorly understood in wheat.Here,we identified 198985 DSCNVs by investigating 491 genomes of common wheat and found that they account for 20%of the entire genome.Interestingly,approximately 38%of genes are linked to DSCNVs.The number of DSCNVs within each accession ranges from 47366 to 96342,and their total sizes vary from 421.3 to 1267.9 Mb.We found that 957 and 1304 DSCNVs have been favored by breeders in China and the United States,respectively.By conducting DSCNV-based genome-wide association studies for the principal components of plant developmental and yield-component traits,we identified 34 loci as directly or indirectly involved in controlling the formation of multiple traits.Notably,a newly discovered DSCNV covering TaFT-D1 is significantly associated with flowering time and other agronomic traits.Overall,our findings highlight the potential of DSCNVs to drive fundamental discoveries in plant science.The comprehensive DSCNV map and the DSCNV-associated genes will also facilitate future research efforts to improve wheat yield,quality,and adaptation.展开更多
Iron is an essential microelement for plant growth.After uptake from the soil,iron is chelated by ligands and translocated from roots to shoots for sub-sequent utilization.However,the number of ligands in-volved in ir...Iron is an essential microelement for plant growth.After uptake from the soil,iron is chelated by ligands and translocated from roots to shoots for sub-sequent utilization.However,the number of ligands in-volved in iron chelation is unclear.In this study,we identified and demonstrated that GLU1,which encodes a ferredoxin-dependent glutamate synthase,was involved in iron homeostasis.First,the expression of GLU1 was strongly induced by iron deficiency condition.Second,lesion of GLU1 results in reduced transcription of many iron-deficiency-responsive genes in roots and shoots.The mutant plants revealed a decreased iron concentration in the shoots,and displayed severe leaf chlorosis under the condition of Fe limitation,compared to wild-type.Third,the product of GLU1,glutamate,could chelate iron in vivo and promote iron transportation.Last,we also found that supplementation of glutamate in the medium can alleviate cadmium toxicity in plants.Overall,our results provide evidence that GLU1 is involved in iron homeo-stasis through affecting glutamate synthesis under iron deficiency conditions in Arabidopsis.展开更多
基金supported by the National Natural Science Foundation of China(grant nos.31991211,31921005,and 32201745)the Strategic Pri-ority Research Program of the Chinese Academy of Sciences(XDA24010104)+1 种基金the Hainan Seed Industry Laboratory(B23C1000402)the Major Basic Research Program of Shandong Natural Science Foundation(ZR2019ZD15).
文摘DNA-segment copy number variations(DSCNVs),such as deletions and duplications,are important sources of genomic structural variation.However,the types and sizes of DSCNVs,as well as their genome-wide distribution and potential functions,are poorly understood in wheat.Here,we identified 198985 DSCNVs by investigating 491 genomes of common wheat and found that they account for 20%of the entire genome.Interestingly,approximately 38%of genes are linked to DSCNVs.The number of DSCNVs within each accession ranges from 47366 to 96342,and their total sizes vary from 421.3 to 1267.9 Mb.We found that 957 and 1304 DSCNVs have been favored by breeders in China and the United States,respectively.By conducting DSCNV-based genome-wide association studies for the principal components of plant developmental and yield-component traits,we identified 34 loci as directly or indirectly involved in controlling the formation of multiple traits.Notably,a newly discovered DSCNV covering TaFT-D1 is significantly associated with flowering time and other agronomic traits.Overall,our findings highlight the potential of DSCNVs to drive fundamental discoveries in plant science.The comprehensive DSCNV map and the DSCNV-associated genes will also facilitate future research efforts to improve wheat yield,quality,and adaptation.
基金This work was supported by the National Key Research and Development Program of China(2016YFD0100706)the National Natural Science Foundation of China(31870225).
文摘Iron is an essential microelement for plant growth.After uptake from the soil,iron is chelated by ligands and translocated from roots to shoots for sub-sequent utilization.However,the number of ligands in-volved in iron chelation is unclear.In this study,we identified and demonstrated that GLU1,which encodes a ferredoxin-dependent glutamate synthase,was involved in iron homeostasis.First,the expression of GLU1 was strongly induced by iron deficiency condition.Second,lesion of GLU1 results in reduced transcription of many iron-deficiency-responsive genes in roots and shoots.The mutant plants revealed a decreased iron concentration in the shoots,and displayed severe leaf chlorosis under the condition of Fe limitation,compared to wild-type.Third,the product of GLU1,glutamate,could chelate iron in vivo and promote iron transportation.Last,we also found that supplementation of glutamate in the medium can alleviate cadmium toxicity in plants.Overall,our results provide evidence that GLU1 is involved in iron homeo-stasis through affecting glutamate synthesis under iron deficiency conditions in Arabidopsis.
