The pathogenesis-related protein PR10 plays a vital role in plant growth,development,and stress responses.This study systematically identified and analyzed PR10 genes in cultivated peanut(Arachis hypogaea L.),examinin...The pathogenesis-related protein PR10 plays a vital role in plant growth,development,and stress responses.This study systematically identified and analyzed PR10 genes in cultivated peanut(Arachis hypogaea L.),examining their phylogenetic relationships,conserved motifs,gene structures,and syntenic relationships.The analysis identified 54 Ah PR10 genes,which were classified into eight groups based on phylogenetic relationships,supported by gene structure and conserved motif characterization.Analysis of chromosomal distribution and synteny demonstrated that segmental duplications played a crucial role in the expansion of the Ah PR10 gene family.The identified Ah PR10 genes exhibited both constitutive and inducible expression patterns.Significantly,Ah PR10-7,Ah PR10-33,and Ah PR10-41 demonstrated potential importance in peanut resistance to Aspergillus flavus.In vitro fungistatic experiments demonstrated that recombinant Ah PR10-33 effectively inhibited A.flavus mycelial growth.These findings provide valuable insights for future investigations into Ah PR10 functions in protecting peanut from A.flavus infection.展开更多
Photocatalytic hydrogen evolution is of great importance with the proceeding of dual carbon goals,for inorganic catalysts have been explored with high efficiency.The structure and properties of oxide composites might ...Photocatalytic hydrogen evolution is of great importance with the proceeding of dual carbon goals,for inorganic catalysts have been explored with high efficiency.The structure and properties of oxide composites might take advantage of each compound and display an increased activity.In our previous study,boron doped-Cu_(3)NiBaTi_(4)O_(9)possessed a porous structure and its photocurrent response was apparent.To further verify its excellent catalytic activity,Al_(2)O_(3),and SiO_(2) were selected to replace with BaTi_(4)O_(9)to prepare different composites.The physical and chemical features of each sample were characterized with SEM,XRD,XPS,etc.to reveal their structural variations.Correspondingly,the H_(2)evolution rate was investigated with gas chromatography under the sunlight irritation.A distinct hydrogen yield was recorded with prepared samples.Further,the projected density of states analysis was taken through density functional theory calculations to appreciate the conduction band of the composite.The offered electrons during the photocatalytic process and the potential applicability of composites in the field of photocatalysis was verified.展开更多
Dear Editor,Cultivated peanut(peanut in short)(Arachis hypogaea,2n=4x=40,genome type AABB)is a legume crop serving globally as a crucial source of edible oil and protein.Available peanut reference genome sequences obt...Dear Editor,Cultivated peanut(peanut in short)(Arachis hypogaea,2n=4x=40,genome type AABB)is a legume crop serving globally as a crucial source of edible oil and protein.Available peanut reference genome sequences obtained for the cultivars"Tifrunner,""Shitouqi,"and"Fuhuasheng"were assembled or patched using third-generation sequencing technologies and significantly contributed to advancements in peanut genetics and breeding(Bertioli et al.,2019;Chen et al.,2019;Zhuang et al.,2019).However,unresolved gaps persist in these assemblies,particularly in regions of biological importance such as centromeres and telomeres(Supplemental Figure 1),hindering comprehensive exploration of their functional and evolutionary significance.展开更多
Nanopore sensing is developing into a powerful label-free approach to investigate the features of biomolecules at the single-molecule level.When a charged molecule is captured within a nanopore,it modulates the ionic ...Nanopore sensing is developing into a powerful label-free approach to investigate the features of biomolecules at the single-molecule level.When a charged molecule is captured within a nanopore,it modulates the ionic current,which can be recorded in real time to reveal the properties of the target molecule.To date,nanopores have been used to sense a variety of analytes,including DNA,RNA,proteins,enzymes,small molecules,cancer cells,and metal ions,and can also provide information on biomolecular structures.In this review,we highlight the progress made in nanopore sensing over the past five years(2016-2020),and provide an outlook on future developments and directions in the field.展开更多
基金supported by the National Key R&D Program of China(2022YFD1200400)the National Natural Science Foundation of China(32301851)。
文摘The pathogenesis-related protein PR10 plays a vital role in plant growth,development,and stress responses.This study systematically identified and analyzed PR10 genes in cultivated peanut(Arachis hypogaea L.),examining their phylogenetic relationships,conserved motifs,gene structures,and syntenic relationships.The analysis identified 54 Ah PR10 genes,which were classified into eight groups based on phylogenetic relationships,supported by gene structure and conserved motif characterization.Analysis of chromosomal distribution and synteny demonstrated that segmental duplications played a crucial role in the expansion of the Ah PR10 gene family.The identified Ah PR10 genes exhibited both constitutive and inducible expression patterns.Significantly,Ah PR10-7,Ah PR10-33,and Ah PR10-41 demonstrated potential importance in peanut resistance to Aspergillus flavus.In vitro fungistatic experiments demonstrated that recombinant Ah PR10-33 effectively inhibited A.flavus mycelial growth.These findings provide valuable insights for future investigations into Ah PR10 functions in protecting peanut from A.flavus infection.
基金supported by the Natural Science Foundation of Xinjiang Uygur Autonomous Region(2021D01A03)Key Research and Development Projects of Xinjiang Uygur Autonomous Region(2022B02038)+1 种基金Special Training Program for Scientific and Technological Talents of Ethnic Minorities in Xinjiang Uygur Autonomous Region(2020D03025)Project of Tian chi talent leader in Xinjiang Uygur Autonomous Region(2022).
文摘Photocatalytic hydrogen evolution is of great importance with the proceeding of dual carbon goals,for inorganic catalysts have been explored with high efficiency.The structure and properties of oxide composites might take advantage of each compound and display an increased activity.In our previous study,boron doped-Cu_(3)NiBaTi_(4)O_(9)possessed a porous structure and its photocurrent response was apparent.To further verify its excellent catalytic activity,Al_(2)O_(3),and SiO_(2) were selected to replace with BaTi_(4)O_(9)to prepare different composites.The physical and chemical features of each sample were characterized with SEM,XRD,XPS,etc.to reveal their structural variations.Correspondingly,the H_(2)evolution rate was investigated with gas chromatography under the sunlight irritation.A distinct hydrogen yield was recorded with prepared samples.Further,the projected density of states analysis was taken through density functional theory calculations to appreciate the conduction band of the composite.The offered electrons during the photocatalytic process and the potential applicability of composites in the field of photocatalysis was verified.
基金supported by the National Key Research and Development Program(2023YFD1202800)the Key Research Project of the Shennong Laboratory(SN01-2022-03)+4 种基金the China Agriculture Research System of MOF and MARA(CARS-13)Key Projects of Henan Province(222301420026)Major Science and Technology Projects of Henan Province(221100110300)the Fund for Distinguished Young Scholars from Henan Academy of Agricultural Sciences(2025ZC13,2023JQ02 and 2024JQ06)the Henan Provincial Agriculture Research System,China(S2012-5).
文摘Dear Editor,Cultivated peanut(peanut in short)(Arachis hypogaea,2n=4x=40,genome type AABB)is a legume crop serving globally as a crucial source of edible oil and protein.Available peanut reference genome sequences obtained for the cultivars"Tifrunner,""Shitouqi,"and"Fuhuasheng"were assembled or patched using third-generation sequencing technologies and significantly contributed to advancements in peanut genetics and breeding(Bertioli et al.,2019;Chen et al.,2019;Zhuang et al.,2019).However,unresolved gaps persist in these assemblies,particularly in regions of biological importance such as centromeres and telomeres(Supplemental Figure 1),hindering comprehensive exploration of their functional and evolutionary significance.
基金This work was supported by the National Natural Science Foundation of China(21874062,22076073)Collaborative Innovation Center in Universities of Shandong Province(Linyi University)the Natural Science Foundation of Shandong Province(ZR2017ZC0226).
文摘Nanopore sensing is developing into a powerful label-free approach to investigate the features of biomolecules at the single-molecule level.When a charged molecule is captured within a nanopore,it modulates the ionic current,which can be recorded in real time to reveal the properties of the target molecule.To date,nanopores have been used to sense a variety of analytes,including DNA,RNA,proteins,enzymes,small molecules,cancer cells,and metal ions,and can also provide information on biomolecular structures.In this review,we highlight the progress made in nanopore sensing over the past five years(2016-2020),and provide an outlook on future developments and directions in the field.
