Banana fruit ripening is a highly regulatory process involving various layers consisting of transcriptional regulation,epigenetic factor,and post-translational modification.Previously,we reported that MaERF11 cooperat...Banana fruit ripening is a highly regulatory process involving various layers consisting of transcriptional regulation,epigenetic factor,and post-translational modification.Previously,we reported that MaERF11 cooperated with MaHDA1 to precisely regulate the transcription of ripening-associated genes via histone deacetylation.However,whether MaERF11 is subjected to post-translational modification during banana ripening is largely unknown.In this study,we found that MaERF11 targeted a subset of starch degradation-related genes using the DNA affinity purification sequence(DAP-Seq)approach.Electrophoretic mobility shift assay(EMSA)and dual-luciferase reporter assay(DLR)demonstrated that MaERF11 could specifically bind and repress the expression of the starch degradation-related genes MaAMY3,MaBAM2 and MaGWD1.Further analyses of yeast two-hybrid(Y2H),bimolecular fluorescence complementation(BiFC)and Luciferase complementation imaging(LCI)assays indicated that MaERF11 interacted with the ubiquitin E3 ligase MaRFA1,and this interaction weakened the MaERF11-mediated transcriptional repression capacity.Collectively,our results suggest an additional regulatory layer in which MaERF11 regulates banana fruit ripening and expands the regulatory network in fruit ripening at the post-translational modification level.展开更多
Plant viral diseases cause great losses in agricultural production.Virus cross-protection is a strategy in which a mild virus is employed to shield plants against subsequent infections by severe viral strains.However,...Plant viral diseases cause great losses in agricultural production.Virus cross-protection is a strategy in which a mild virus is employed to shield plants against subsequent infections by severe viral strains.However,this approach is restricted to protection against the same viruses.In this study,we observed that pre-inoculation with apple geminivirus(AGV)reduced the accumulation of secondarily infected heterologous viruses,such as cucumber mosaic virus,potato virus X,and tobacco mosaic virus in Nicotiana benthamiana,tomato,and pepper plants.Transcriptional expression analysis showed that autophagy-related genes were transcriptionally up-regulated upon AGV inoculation at an early stage of infection.Accordingly,autophagic activity was observed to be elevated following AGV infection.Interestingly,AGV accumulation was reduced in autophagy-deficient plants,suggesting that autophagy activation promotes AGV infection in the plant.Moreover,pre-inoculation with AGV provided cross-protection against infection with a phytopathogenic bacterium(Pseudomonas syringae)and fungus(Botrytis cinerea)in Nicotiana species.In summary,our study showed that AGV,an asymptomatic virus,could protect plants against severe viral,fungal,and bacterial diseases to some extent through the activation of autophagy pathways,highlighting its potential as a biocontrol agent for managing a wide range of plant crop diseases in the field.展开更多
基金financially supported by the National Natural Science Foundation of China(Grant Nos.31830071,32202561)the earmarked fund for CARS(Grant No.CARS-31)。
文摘Banana fruit ripening is a highly regulatory process involving various layers consisting of transcriptional regulation,epigenetic factor,and post-translational modification.Previously,we reported that MaERF11 cooperated with MaHDA1 to precisely regulate the transcription of ripening-associated genes via histone deacetylation.However,whether MaERF11 is subjected to post-translational modification during banana ripening is largely unknown.In this study,we found that MaERF11 targeted a subset of starch degradation-related genes using the DNA affinity purification sequence(DAP-Seq)approach.Electrophoretic mobility shift assay(EMSA)and dual-luciferase reporter assay(DLR)demonstrated that MaERF11 could specifically bind and repress the expression of the starch degradation-related genes MaAMY3,MaBAM2 and MaGWD1.Further analyses of yeast two-hybrid(Y2H),bimolecular fluorescence complementation(BiFC)and Luciferase complementation imaging(LCI)assays indicated that MaERF11 interacted with the ubiquitin E3 ligase MaRFA1,and this interaction weakened the MaERF11-mediated transcriptional repression capacity.Collectively,our results suggest an additional regulatory layer in which MaERF11 regulates banana fruit ripening and expands the regulatory network in fruit ripening at the post-translational modification level.
基金supported by the National Natural Science Foundation of China(31970163)to LSMajor Science and Technology Project of Beijing Municipal Education Commission(Grant no.KZ202210020028)to QS.
文摘Plant viral diseases cause great losses in agricultural production.Virus cross-protection is a strategy in which a mild virus is employed to shield plants against subsequent infections by severe viral strains.However,this approach is restricted to protection against the same viruses.In this study,we observed that pre-inoculation with apple geminivirus(AGV)reduced the accumulation of secondarily infected heterologous viruses,such as cucumber mosaic virus,potato virus X,and tobacco mosaic virus in Nicotiana benthamiana,tomato,and pepper plants.Transcriptional expression analysis showed that autophagy-related genes were transcriptionally up-regulated upon AGV inoculation at an early stage of infection.Accordingly,autophagic activity was observed to be elevated following AGV infection.Interestingly,AGV accumulation was reduced in autophagy-deficient plants,suggesting that autophagy activation promotes AGV infection in the plant.Moreover,pre-inoculation with AGV provided cross-protection against infection with a phytopathogenic bacterium(Pseudomonas syringae)and fungus(Botrytis cinerea)in Nicotiana species.In summary,our study showed that AGV,an asymptomatic virus,could protect plants against severe viral,fungal,and bacterial diseases to some extent through the activation of autophagy pathways,highlighting its potential as a biocontrol agent for managing a wide range of plant crop diseases in the field.
