Alzheimer's disease(AD)is a serious disease associated with cognitive impairment,and synaptic loss and amyloid-beta(Aβ)deposition are closely related to its pathogenesis.D ocosahexaenoic acid(DHA)has been reporte...Alzheimer's disease(AD)is a serious disease associated with cognitive impairment,and synaptic loss and amyloid-beta(Aβ)deposition are closely related to its pathogenesis.D ocosahexaenoic acid(DHA)has been reported to alter the cognitive impairment associated with aging and reduce the risk of long-term development of AD.However,the effects of Aβon synapses and whether DHA has a protective effect on synapses remain unclear.In this study,APPSwe/PSEN1d E9 transgenic and wild type mice were used as experimental subjects to explore the effect of DHA on synaptic structure and function damaged by Aβ.Results showed that a large amount of Aβwas deposited in the brain of AD mice,and DHA intervention decreased the Aβdeposition(P<0.05).DHA decreased the apoptosis of nerve cells and the oxidative stress level induced by Aβ(P<0.01).Transmission electron microscopy results showed that DHA improved the synaptic structure and number,and the expression of synaptophysin was significantly upregulated by DHA(P<0.01).Besides,neurotransmitter release was regulated after DHA intervention,including the decreased Glu level and increasedγ-aminobutyric acid level,as well as the activity of ATPase was also increased by DHA.Importantly,the phosphorylation levels of tyrosine kinase B(Trk B),phospholipase C-gamma-1,calcium/calmodulin-dependent protein kinase II,extracellular regulated protein kinases(ERK1/2),and c AMP-response element binding protein(CREB)were upregulated by DHA(P<0.01).These data indicated that DHA could improve synaptic structure and function through the Trk B-Erk1/2-CREB pathway,thus playing a positive role in the pathological process of AD.展开更多
目的:基于Web of Science数据库的收录情况,通过文献计量方法分析角膜塑形镜的研究现状和热点。方法:以近10年来(2007年1月至2018年11月)Web of Science数据库收录的角膜塑形镜相关文献为分析对象,运用文献计量学方法,采用CiteSpace工...目的:基于Web of Science数据库的收录情况,通过文献计量方法分析角膜塑形镜的研究现状和热点。方法:以近10年来(2007年1月至2018年11月)Web of Science数据库收录的角膜塑形镜相关文献为分析对象,运用文献计量学方法,采用CiteSpace工具对文献进行可视化分析,对文献发文量,国家及机构分布,核心作者信息,刊文期刊分布及关键词聚类等方面进行综合分析。结果:共检索到401篇文献,相关文献分别刊载在67种期刊上,涉及418位作者;学科发展覆盖36个国家或地区,共345个研究机构参与该领域研究,中国有2所大学排名前5位,拥有较强竞争力;学科发展主要集中在中国、澳大利亚、美国和西班牙这4国,主要研究领域为光学像差、对比敏感度、周边屈光度、随机临床试验以及眼球生长等方面,随着研究深入,研究热点在变化。结论:近10年来,在角膜塑形镜领域,中国的刊文量居于世界领先地位;国内香港理工大学和温州医科大学具有较强的国际竞争力;研究热点从角膜塑形镜配戴对视觉质量和角膜厚度等方面的改变,到控制近视进展的可能机制,逐渐转移到与多种近视控制方法的临床随机对照试验的探究。展开更多
基金supported by Natural Science Foundation of Henan Province of China(242300421536)College Students’Innovation and Entrepreneurship Training Program of Xinxiang Medicine University(202410472024).
文摘Alzheimer's disease(AD)is a serious disease associated with cognitive impairment,and synaptic loss and amyloid-beta(Aβ)deposition are closely related to its pathogenesis.D ocosahexaenoic acid(DHA)has been reported to alter the cognitive impairment associated with aging and reduce the risk of long-term development of AD.However,the effects of Aβon synapses and whether DHA has a protective effect on synapses remain unclear.In this study,APPSwe/PSEN1d E9 transgenic and wild type mice were used as experimental subjects to explore the effect of DHA on synaptic structure and function damaged by Aβ.Results showed that a large amount of Aβwas deposited in the brain of AD mice,and DHA intervention decreased the Aβdeposition(P<0.05).DHA decreased the apoptosis of nerve cells and the oxidative stress level induced by Aβ(P<0.01).Transmission electron microscopy results showed that DHA improved the synaptic structure and number,and the expression of synaptophysin was significantly upregulated by DHA(P<0.01).Besides,neurotransmitter release was regulated after DHA intervention,including the decreased Glu level and increasedγ-aminobutyric acid level,as well as the activity of ATPase was also increased by DHA.Importantly,the phosphorylation levels of tyrosine kinase B(Trk B),phospholipase C-gamma-1,calcium/calmodulin-dependent protein kinase II,extracellular regulated protein kinases(ERK1/2),and c AMP-response element binding protein(CREB)were upregulated by DHA(P<0.01).These data indicated that DHA could improve synaptic structure and function through the Trk B-Erk1/2-CREB pathway,thus playing a positive role in the pathological process of AD.
文摘目的:基于Web of Science数据库的收录情况,通过文献计量方法分析角膜塑形镜的研究现状和热点。方法:以近10年来(2007年1月至2018年11月)Web of Science数据库收录的角膜塑形镜相关文献为分析对象,运用文献计量学方法,采用CiteSpace工具对文献进行可视化分析,对文献发文量,国家及机构分布,核心作者信息,刊文期刊分布及关键词聚类等方面进行综合分析。结果:共检索到401篇文献,相关文献分别刊载在67种期刊上,涉及418位作者;学科发展覆盖36个国家或地区,共345个研究机构参与该领域研究,中国有2所大学排名前5位,拥有较强竞争力;学科发展主要集中在中国、澳大利亚、美国和西班牙这4国,主要研究领域为光学像差、对比敏感度、周边屈光度、随机临床试验以及眼球生长等方面,随着研究深入,研究热点在变化。结论:近10年来,在角膜塑形镜领域,中国的刊文量居于世界领先地位;国内香港理工大学和温州医科大学具有较强的国际竞争力;研究热点从角膜塑形镜配戴对视觉质量和角膜厚度等方面的改变,到控制近视进展的可能机制,逐渐转移到与多种近视控制方法的临床随机对照试验的探究。
