This study determined the lifetime of the first excited state(5∕2_(1)^(+))in ^(139)La via β-γ time-difference measurement using a LaBr_(3)+plastic scintillator array.This state is populated following the decay of ^...This study determined the lifetime of the first excited state(5∕2_(1)^(+))in ^(139)La via β-γ time-difference measurement using a LaBr_(3)+plastic scintillator array.This state is populated following the decay of ^(139)Ba produced in the^(138)Ba(n,γ)reaction.Compared with previous experiments using only stilbene/plastic crystals,this experiment separates the background contribution in the γ-ray spectrum owing to the high energy resolution of LaBr_(3).The L-forbidden M1 transition strength,B(M1,5∕2_(1)^(+)→7∕2_(1)^(+)),in^(139)La was measured and compared with detailed large-scale shell model calculations,with a special focus on the core-excitation effect.The results showed the importance of both proton and neutron core-excitations in explaining the M1 transition strength.Meanwhile,the effective g-factor for the tensor term of the M1 operator was smaller than the previously reported value in this region or around ^(208)Pb.展开更多
Objective:To investigate theanti apoptosis effect of curcumin(cur)in experimental autoimmune encephalomyelitis(EAE)mice by regulating TLRs/NF-κB signaling pathway and its mechanism.Methods:45 C57BL/6 mice were random...Objective:To investigate theanti apoptosis effect of curcumin(cur)in experimental autoimmune encephalomyelitis(EAE)mice by regulating TLRs/NF-κB signaling pathway and its mechanism.Methods:45 C57BL/6 mice were randomly divided into the control group,EAE group,curcumin group,15 mice in each group.Blank groups are not processed.The EAE model was established by classical modeling method in the EAE group and the curcumin group.From the day of modeling,the blank group and the EAE group were intraperitoneally injected with 1ml/kg/d of normal saline,Curcumin group was given 100 mg/kg/d continuous intraperitoneal injection of curcumin extract.With Benson EAE group and Curcumin group mice were killed at the peak of the disease.The blank group and the rest of the mice were killed after 4 weeks of feeding,and the spinal cord tissue was taken out to separate the lumbar enlargement segment.The effects of curcumin on the pathological changes of spinal cord tissue in EAE mice were observed by HE staining and TUNEL staining,and the expression of apoptosis positive cells was calculated.The distribution and co aggregation of apoptosis related proteins Bcl-2 and Bax with spinal cord tissue were observed by double immunofluorescence staining The protein levels of TLR4,NF-κBp65 and MyD88 were detected by Western blot.Results:compared with the blank group,TUNEL staining increased the number of apoptotic cells and the apoptotic rate in EAE group(P<0.05);the expression of apoptosis related protein Bcl-2 decreased and the expression of Bax increased in EAE group(P<0.05),The protein of TLR4,NF-κBp65 and MyD88 in spinal cord tissue of mice were increased by blot detection(P<0.05);compared with EAE group,the number of apoptotic cells in spinal cord tissue of curcumin group was decreased by TUNEL staining,and the apoptosis rate was decreased(P<0.05);the expression of apoptosis related protein Bcl-2 was increased,the expression of Bax protein was decreased,and Western blot was used to detect the expression of apoptosis related protein The protein of TLR4,NF-κBp65 and MyD88 in spinal cord tissue of mice were decreased by blot(P<0.05).Conclusion:Curcumin has anti apoptotic effect on EAE mice,and its mechanism may be related to the inhibition of TLRs/NF-κB signaling pathway and the reduction of apoptotic protein production.展开更多
基金supported by the Guangdong Major Project of Basic and Applied Basic Research (No. 2021B0301030006)Young Scientists Fund of the National Natural Science Foundation of China (No. 12405144)+3 种基金the National Natural Science Foundation of China (No. 12475129)the International Atomic Energy Agency Coordinatated Research Project F41034 (No. 28649)the computational resources from Sun Yat-sen University the National Supercomputer Center in Guangzhouthe Natural Science Foundation of Guangdong Province,China (No. 2025A1515012112)
文摘This study determined the lifetime of the first excited state(5∕2_(1)^(+))in ^(139)La via β-γ time-difference measurement using a LaBr_(3)+plastic scintillator array.This state is populated following the decay of ^(139)Ba produced in the^(138)Ba(n,γ)reaction.Compared with previous experiments using only stilbene/plastic crystals,this experiment separates the background contribution in the γ-ray spectrum owing to the high energy resolution of LaBr_(3).The L-forbidden M1 transition strength,B(M1,5∕2_(1)^(+)→7∕2_(1)^(+)),in^(139)La was measured and compared with detailed large-scale shell model calculations,with a special focus on the core-excitation effect.The results showed the importance of both proton and neutron core-excitations in explaining the M1 transition strength.Meanwhile,the effective g-factor for the tensor term of the M1 operator was smaller than the previously reported value in this region or around ^(208)Pb.
基金Luzhou Municipal Government(No.2018LZXNYD-ZK17)。
文摘Objective:To investigate theanti apoptosis effect of curcumin(cur)in experimental autoimmune encephalomyelitis(EAE)mice by regulating TLRs/NF-κB signaling pathway and its mechanism.Methods:45 C57BL/6 mice were randomly divided into the control group,EAE group,curcumin group,15 mice in each group.Blank groups are not processed.The EAE model was established by classical modeling method in the EAE group and the curcumin group.From the day of modeling,the blank group and the EAE group were intraperitoneally injected with 1ml/kg/d of normal saline,Curcumin group was given 100 mg/kg/d continuous intraperitoneal injection of curcumin extract.With Benson EAE group and Curcumin group mice were killed at the peak of the disease.The blank group and the rest of the mice were killed after 4 weeks of feeding,and the spinal cord tissue was taken out to separate the lumbar enlargement segment.The effects of curcumin on the pathological changes of spinal cord tissue in EAE mice were observed by HE staining and TUNEL staining,and the expression of apoptosis positive cells was calculated.The distribution and co aggregation of apoptosis related proteins Bcl-2 and Bax with spinal cord tissue were observed by double immunofluorescence staining The protein levels of TLR4,NF-κBp65 and MyD88 were detected by Western blot.Results:compared with the blank group,TUNEL staining increased the number of apoptotic cells and the apoptotic rate in EAE group(P<0.05);the expression of apoptosis related protein Bcl-2 decreased and the expression of Bax increased in EAE group(P<0.05),The protein of TLR4,NF-κBp65 and MyD88 in spinal cord tissue of mice were increased by blot detection(P<0.05);compared with EAE group,the number of apoptotic cells in spinal cord tissue of curcumin group was decreased by TUNEL staining,and the apoptosis rate was decreased(P<0.05);the expression of apoptosis related protein Bcl-2 was increased,the expression of Bax protein was decreased,and Western blot was used to detect the expression of apoptosis related protein The protein of TLR4,NF-κBp65 and MyD88 in spinal cord tissue of mice were decreased by blot(P<0.05).Conclusion:Curcumin has anti apoptotic effect on EAE mice,and its mechanism may be related to the inhibition of TLRs/NF-κB signaling pathway and the reduction of apoptotic protein production.
