BACKGROUND Metabolic-associated fatty liver disease(MAFLD)is characterized by lipid accumulation in hepatocytes and is closely associated with oxidative stress.Increasing clinical evidence indicates that MAFLD is link...BACKGROUND Metabolic-associated fatty liver disease(MAFLD)is characterized by lipid accumulation in hepatocytes and is closely associated with oxidative stress.Increasing clinical evidence indicates that MAFLD is linked to bone metabolic disorders,including osteoporosis.Recent studies indicate that the expression profiles of liver circular RNAs(circRNAs)are altered in MAFLD.However,the effects of these changes on bone metabolism remain poorly understood.AIM To investigate the effects and mechanism of differently expressed circRNAs secreted by the liver on osteogenic differentiation in MAFLD.METHODS RNA sequencing was performed to identify highly expressed circRNAs in the liver,validated by quantitative real-time reverse transcription polymerase chain reaction,and localized using fluorescence in situ hybridization(FISH).A mouse model induced by a high-fat diet was used to simulate MAFLD.RESULTS CircSOD2 was significantly upregulated in liver tissues and primary hepatocytes from subjects with MAFLD.CircSOD2 was induced by oxidative stress and attenuated by antioxidants in the mouse model.In addition,circSOD2 was delivered from hepatocytes to bone marrow mesenchymal stem cells(BMSCs).Furthermore,circSOD2 inhibited the osteogenic differentiation of BMSCs and in vivo bone formation by sponging miR-29b.Moreover,miR-29b inhibition reversed the stimulatory effect of circSOD2 silencing on osteogenic differentiation of BMSCs and in vivo bone formation.Mechanistically,the interaction between circSOD2 and miR-29b confirmed through a luciferase reporter assay and the co-localization in the cytoplasm evidenced by FISH indicated that circSOD2 acted as a sponge for miR-29b.CONCLUSION This study provides a novel mechanism underlying the liver-bone crosstalk,demonstrating that circSOD2 upregulation in hepatocytes,induced by oxidative stress,inhibits osteogenic differentiation of BMSCs by sponging miR-29b.These findings offer a better understanding of the relationship between MAFLD and osteoporosis.展开更多
BACKGROUND Liver cirrhosis and portal hypertension(PHT)can lead to lymphatic abnormalities and coagulation dysfunction.Because lymphangiogenesis may relieve liver cirrhosis and PHT,the present study investigated the g...BACKGROUND Liver cirrhosis and portal hypertension(PHT)can lead to lymphatic abnormalities and coagulation dysfunction.Because lymphangiogenesis may relieve liver cirrhosis and PHT,the present study investigated the gene expression alterations in the lymphatic system and the effectiveness of platelet-mediated lymphangiogenesis in improving liver cirrhosis and PHT.AIM To investigate the role of lymphangiogenesis in preclinical PHT models.METHODS Immunohistochemistry and transcriptome sequencing of bile duct ligation(BDL)and control lymphatic samples were conducted to reveal the indicated signaling pathways.Functional enrichment analyses were performed on the differentially expressed genes and hub genes.Adenoviral infection of vascular endothelial growth factor C(VEGF-C),plateletrich plasma(PRP),and VEGF3 receptor(VEGFR)inhibitor MAZ-51 was used as an intervention for the lymphatic system in PHT models.Histology,hemodynamic tests and western blot analyses were performed to demonstrate the effects of lymphatic intervention in PHT patients.RESULTS Lymphangiogenesis was increased in the BDL rat model.Transcriptome sequencing analysis of the extrahepatic lymphatic system revealed its close association with platelet adherence,aggregation,and activation.The role of PHT in the rat model was investigated by activating(PRP)and inhibiting(MAZ-51)the lymphatic system.PRP promoted lymphangiogenesis,which increased lymphatic drainage,alleviated portal pressure,reduced liver fibrosis,inhibited inflammation,inhibited angiogenesis,and suppressed mesenteric artery remodeling.MAZ-51 reversed the above improvements.CONCLUSION Via VEGF-C/VEGFR-3,platelets impede fibrosis,angiogenesis,and mesenteric artery remodeling,ultimately alleviating PHT.Thus,platelet intervention is a therapeutic approach for cirrhosis and PHT.展开更多
The introduction of highly polarized flexible segments into polymer molecular chains is an effective means to improve the dielectric constant and mechanical flexibility of polymers,which is important for improving the...The introduction of highly polarized flexible segments into polymer molecular chains is an effective means to improve the dielectric constant and mechanical flexibility of polymers,which is important for improving the energy storage characteristics and applicability for the roll-to-roll process of metallic film capacitors.However,the introduction of flexible segments may adversely affect the heat resistance of polymer materials,which limits their application at high working temperatures.In this work,we have prepared a novel epoxy film with excellent comprehensive properties by introducing rigid phenyl and flexible etherified methylene side chains.For instance,the optimal epoxy film showed an energy storage density of 7.06 J cm^(-3)and 85%charge-discharge efficiency at room temperature and 420 kV mm^(-1).At 200 kV mm^(-1)and 110°C,a working condition for the application of the electric vehicle,the prepared film still showed an energy storage density of 1.5 J cm^(-3)and charge-discharge efficiency of 86%,which is 3 times that of BOPP film.This work provides an idea for material designs of high-performance polymer film capacitors.展开更多
Polymer-based composite electrolytes composed of three-dimensional Li_(6.4)La_(3)Zr_(2)Al_(0.2)O_(12)(3D-LLZAO)have attracted increasing attention due to their continuous ion conduction and satisfactory mechanical pro...Polymer-based composite electrolytes composed of three-dimensional Li_(6.4)La_(3)Zr_(2)Al_(0.2)O_(12)(3D-LLZAO)have attracted increasing attention due to their continuous ion conduction and satisfactory mechanical properties.However,the organic/inorganic interface is incompatible,resulting in slow lithium-ion transport at the interface.Therefore,the compatibility of organic/inorganic interface is an urgent problem to be solved.Inspired by the concept of“gecko eaves”,polymer-based composite solid electrolytes with dense interface structures were designed.The bridging of organic/inorganic interfaces was established by introducing silane coupling agent(3-chloropropyl)trimethoxysilane(CTMS)into the PEO-3D-LLZAO(PL)electrolyte.The in-situ coupling reaction improves the interface affinity,strengthens the organic/inorganic interaction,reduces the interface resistance,and thus achieves an efficient interface ion transport network.The prepared PEO-3D-LLZAO-CTMS(PLC)electrolyte exhibits enhanced ionic conductivity of 6.04×10^(-4)S cm^(-1)and high ion migration number(0.61)at 60℃and broadens the electrochemical window(5.1 V).At the same time,the PLC electrolyte has good thermal stability and high mechanical properties.Moreover,the Li Fe PO_(4)|PLC|Li battery has excellent rate performance and cycling stability with a capacity decay rate of 2.2%after 100 cycles at 60℃and 0.1 C.These advantages of PLC membranes indicate that this design approach is indeed practical,and the in-situ coupling method provides a new approach to address interface compatibility issues.展开更多
The changes of protein secondary structures in the extracellular polymeric substances(EPS) extracted from activated sludge by four different methods were studied by analyzing the amide I region(1700–1600 cm-1) of the...The changes of protein secondary structures in the extracellular polymeric substances(EPS) extracted from activated sludge by four different methods were studied by analyzing the amide I region(1700–1600 cm-1) of the Fourier transform infrared spectra and model protein test. The results showed the molecular weight distribution of organic matter extracted by centrifugation, heating and cation exchange resin(CER) was similar, while the EPS extracted by centrifugation(Control) and CER had similar fluorescent organic matter. The protein secondary structures of extracted EPS by the four methods were different. The similarities of protein secondary structures between the EPS extracted by CER with the Control were the highest among the four extracted EPS. Although the EPS yield extracted by formaldehyde + NaOH method were the highest, its protein secondary structures had the lowest similarity with those extracted by the Control. Additionally, the effects of centrifugation and CER extraction on the secondary structures of bovine serum albumin were also lower than that of other extraction processes. CER enables the second maximum extraction of EPS and maximum retention of the original secondary structure of proteins.展开更多
Quiescent state has been observed in stem cells(SCs),including in adult SCs and in cancer SCs(CSCs).Quiescent status of SCs contributes to SC self-renewal and conduces to averting SC death from harsh external stimuli....Quiescent state has been observed in stem cells(SCs),including in adult SCs and in cancer SCs(CSCs).Quiescent status of SCs contributes to SC self-renewal and conduces to averting SC death from harsh external stimuli.In this review,we provide an overview of intrinsic mechanisms and extrinsic factors that regulate adult SC quiescence.The intrinsic mechanisms discussed here include the cell cycle,mitogenic signaling,Notch signaling,epigenetic modification,and metabolism and transcriptional regulation,while the extrinsic factors summarized here include microenvironment cells,extracellular factors,and immune response and inflammation in microenvironment.Quiescent state of CSCs has been known to contribute immensely to therapeutic resistance in multiple cancers.The characteristics and the regulation mechanisms of quiescent CSCs are discussed in detail.Importantly,we also outline the recent advances and controversies in therapeutic strategies targeting CSC quiescence.展开更多
Objective： A number of studies have reported the association of ＂XPA＂, ＂XPC＂, ＂XPD/ERCC2＂ gene polymorphisms with lung cancer risk. However, the results were conflict. To clarify the impact of polymorphisms of ...Objective： A number of studies have reported the association of ＂XPA＂, ＂XPC＂, ＂XPD/ERCC2＂ gene polymorphisms with lung cancer risk. However, the results were conflict. To clarify the impact of polymorphisms of ＂XPA＂, ＂XPC＂, ＂XPD/ERCC2＂, on lung cancer risk, a meta-analysis was performed in this study. Methods： The electronic databases PubMed and Embase were retrieved for studies included in this meta-analysis by ＂XPA＂, ＂XPC＂, ＂XPD/ERCC2＂, ＂lung＂, ＂cancer/neoplasm/tumor/carcinoma＂, ＂polymorphism＂ （An upper date limit of October, 31, 2009）. A meta-analysis was performed to evaluate the relationship among XPA, XPC and XPD polymorphism and lung cancer risks. Results： A total of 31 publications retrieved from Pubmed and Embase included in this study. XPC A939C CC genotype increased lung cancer risk in total population （recessive genetic model： OR=1.23, 95% CI：1.05-1.44; homozygote comparison： OR=1.21,95%CI：1.02-1.43and CC vs. CA contrast： OR=1.25,95%CI：1.06-1.48）, except in Asians. XPD A751C, 751C allele and CC genotype also increased lung cancer risk in total population and in Caucasians （recessive genetic model： Total population： OR=1.20, 95%CI：1.07-1.35）. No significant correlation was found between XPD A751C and lung cancer risk in Asians and African Americans. XPD G312A AA genotype increased lung cancer risk in total population, in Asians and Caucasians（recessive genetic model： Total population： OR=1.20, 95%CI： 1.06-1.36）. No significant association was found between XPA G23A, XPC C499T, XPD C156A and lung cancer risk. Conclusion： Our results suggest that the polymorphisms in XPC and XPD involve in lung cancer risks. XPA polymorphisms is less related to lung cancer risk.展开更多
Automatic mode-locking techniques,the integration of intelligent technologies with nonlinear optics offers the promise of on-demand intelligent control,potentially overcoming the inherent limitations of traditional ul...Automatic mode-locking techniques,the integration of intelligent technologies with nonlinear optics offers the promise of on-demand intelligent control,potentially overcoming the inherent limitations of traditional ultrafast pulse generation that have predominantly suffered from the instability and suboptimality of open-loop manual tuning.The advancements in intelligent algorithm-driven automatic mode-locking techniques primarily are explored in this review,which also revisits the fundamental principles of nonlinear optical absorption,and examines the evolution and categorization of conventional mode-locking techniques.The convergence of ultrafast pulse nonlinear interactions with intelligent technologies has intricately expanded the scope of ultrafast photonics,unveiling considerable potential for innovation and catalyzing new waves of research breakthroughs in ultrafast photonics and nonlinear optics characters.展开更多
Triple-negative breast cancer(TNBC)is an inherently heterogeneous malignancy at the biological and clinical levels,occupying around 15%of diagnosed breast cancer cases and being the most lethal breast cancer subtype t...Triple-negative breast cancer(TNBC)is an inherently heterogeneous malignancy at the biological and clinical levels,occupying around 15%of diagnosed breast cancer cases and being the most lethal breast cancer subtype to combat.1 Anoikis is a programmed cell death that occurs when cells detach from the correct extracellular matrix,thereby destroying integrin connections.2 However,the role of anoikis expression pattern and prognosis in TNBC is still unrevealed.Here,TNBC presented three heterogeneous phenotypes in anoikis,and each subtype owned unique molecular,and clinical characteristics as well as diverse responses to chemotherapy,targeted therapies,and immune checkpoint blockade.The anoikis-based scoring system can accurately estimate patient survival,which was remarkably superior to well-established clinical parameters.展开更多
Breast cancer(BRCA)is the dominating form of cancer affecting women,with an estimated 2.3 million newly diagnosed cases and 685,000 deaths in 2020.1 An emerging study has proposed a novel metabolism-related regulated ...Breast cancer(BRCA)is the dominating form of cancer affecting women,with an estimated 2.3 million newly diagnosed cases and 685,000 deaths in 2020.1 An emerging study has proposed a novel metabolism-related regulated cell'death modality called disulfidptosis induced by abnormal_accumulation_of intracellular disulfides in SLC7A11high tumor cells.2 However,research on disulfidptosis remains in its infancy.The study presents for the first time a comprehensive investigation on disulfidptosis in BRCA.展开更多
Acidity-activatable magnetic resonance imaging(MRI)nanoprobes offer great potential for in vivo cancer imaging by targeting the acidic tumor microenvironment(TME).However,their effectiveness is limited by the delayed ...Acidity-activatable magnetic resonance imaging(MRI)nanoprobes offer great potential for in vivo cancer imaging by targeting the acidic tumor microenvironment(TME).However,their effectiveness is limited by the delayed response at tumor sites and uncontrollable background noise,compromising imaging accuracy and reliability.Herein,an acidic TME-responsive nanoprobe,SPIO@ZIF-8@Gd(SZG),with dually activatable T_(1) and T_(2) MR signals is shown for acidity-selective contrast enhancement in a rapid response manner.It shows decreased T_(1) and T_(2) contrast intensity in normal physiological conditions.Once targeting acidic TME,the zeolitic imidazolate framework-8(ZIF-8)layer undergoes instantaneous decomposition,releasing Gd^(3+)(T_(1)-weighted),and exposing the inner SPIO(T_(2)-weighted)core,thereby sequentially recovering the signals.Compared to previously reported T_(1)-T_(2) nanoprobes,SZG demonstrates noticeable“dual activation”after just 30 min and reaches its peak 4 h after acid incubation.Additionally,it shows an excellent“acidity correlation”between relaxation times and pH values.When the SZG nanoprobe is used combined with“dual-contrast enhanced subtraction(DESI)”,the contrast difference between diseased and normal tissue can be increased by 10 times,which is significantly higher than traditional single-mode T_(1)/T_(2) contrast agents.Collectively,these findings demonstrate a rapid imaging strategy of dual-activation MR imaging of the acidic TME and simultaneous background suppression,thus paving the way for precise tumor malignancy differentiation,early tumor detection,and accurate tumor grading.展开更多
Ductal carcinoma in situ(DCIS)represents pre-invasive breast carcinoma.In untreated cases,25-60%DCIS progress to invasive ductal carcinoma(IDC).The challenge lies in distinguishing between non-progressive and progress...Ductal carcinoma in situ(DCIS)represents pre-invasive breast carcinoma.In untreated cases,25-60%DCIS progress to invasive ductal carcinoma(IDC).The challenge lies in distinguishing between non-progressive and progressive DCIS,often resulting in over-or under-treatment in many cases.With increasing screen-detected DCIS in these years,the nature of DCIS has aroused worldwide attention.A deeper understanding of the biological nature of DCIS and the molecular journey of the DCIS-IDC transition is crucial for more effective clinical management.Here,we reviewed the key signaling pathways in breast cancer that may contribute to DCIS initiation and progression.We also explored the molecular features of DCIS and IDC,shedding light on the progression of DCIS through both inherent changes within tumor cells and alterations in the tumor microenvironment.In addition,valuable research tools utilized in studying DCIS including preclinical models and newer advanced technologies such as single-cell sequencing,spatial transcriptomics and artificial intelligence,have been systematically summarized.Further,we thoroughly discussed the clinical advancements in DCIS and IDC,including prognostic biomarkers and clinical managements,with the aim of facilitating more personalized treatment strategies in the future.Research on DCIS has already yielded significant insights into breast carcinogenesis and will continue to pave the way for practical clinical applications.展开更多
Circulating tumor cells(CTCs)are tumor cells that have sloughed off the primary tumor and extravasate into and circulate in the blood.Understanding of the metastatic cascade of CTCs has tremendous potential for the id...Circulating tumor cells(CTCs)are tumor cells that have sloughed off the primary tumor and extravasate into and circulate in the blood.Understanding of the metastatic cascade of CTCs has tremendous potential for the identification of targets against cancer metastasis.Detecting these very rare CTCs among the massive blood cells is challenging.However,emerging technologies for CTCs detection have profoundly contributed to deepening investigation into the biology of CTCs and have facilitated their clinical application.Current technologies for the detection of CTCs are summarized herein,together with their advantages and disadvantages.The detection of CTCs is usually dependent on molecular markers,with the epithelial cell adhesion molecule being the most widely used,although molecular markers vary between different types of cancer.Properties associated with epithelial-tomesenchymal transition and stemness have been identified in CTCs,indicating their increased metastatic capacity.Only a small proportion of CTCs can survive and eventually initiate metastases,suggesting that an interaction and modulation between CTCs and the hostile blood microenvironment is essential for CTC metastasis.Single-cell sequencing of CTCs has been extensively investigated,and has enabled researchers to reveal the genome and transcriptome of CTCs.Herein,we also review the clinical applications of CTCs,especially for monitoring response to cancer treatment and in evaluating prognosis.Hence,CTCs have and will continue to contribute to providing significant insights into metastatic processes and will open new avenues for useful clinical applications.展开更多
Flexible strain sensors exhibit outstanding advantages in terms of sensitivity and stability by detecting changes in physical signals.It can be easily attached to human skin and clothed to achieve monitoring of human ...Flexible strain sensors exhibit outstanding advantages in terms of sensitivity and stability by detecting changes in physical signals.It can be easily attached to human skin and clothed to achieve monitoring of human motion and health.However,general sensing material shows low stretchability and cannot respond to signals under large deformation.In this work,a highly stretchable polymer composite was developed by adding small amount(0.17 wt.%)of silver nanowires(AgNWs)in stretchable conductive polymer materials.The conductivity of polymer/AgNWs composite is 1.3 S/m with the stretchability up to 500%.The stretchable strain sensor based on the polymer/AgNWs composite can respond to strain signals in real time,even for 1%strain response,and shows excellent stability over 1,000 loading/unloading cycles.Moreover,the strain sensor can be attached to human skin and clothed to monitor joints,throat and pulse of the human body.The human body electrocardiogram(ECG)signal was detected successfully with the polymer/AgNWs electrode,which is comparable to the signal obtained by the commercial electrode.Overall,the sensors enable monitoring of human movement and health.These advantages make it a potential application in wearable devices and electronic skin.展开更多
The efficient cutaneous wound healing accompanied with the enhanced skin appendage regeneration is still a challenge.The bacterial infection and excessive/prolonged inflammation inhibit wound healing process and resul...The efficient cutaneous wound healing accompanied with the enhanced skin appendage regeneration is still a challenge.The bacterial infection and excessive/prolonged inflammation inhibit wound healing process and result in the scar formation.Herein,we reported an anti-inflammatory polycitrate-polyethyleneimine-Ibuprofen(PCEI)and multifunctional PCEI-based F127-ε-polypeptide-alginic(FEA)dressing(FEA-PCEI)for accelerating wound healing and hair follicle neogenesis.PCEI showed the excellent anti-inflammation function through stimulating macrophage towards anti-inflammatory M2 subtype polarization.The FEA-PCEI dressing showed the temperature-response gelation,injectability,robust antibacterial activity,light-damage-resistant,homeostasis ability,and good cytocompatibility.The optimized dosage of FEA-PCEI dressing could significantly accelerate wound healing with anti-infection ability,reduce the scar formation,and promote the hair follicle neogenesis.This study provided a wound-repairing strategy through regulating the phenotype of immune cells by the designing bioactive multifunctional biomaterials.展开更多
Excessive reactive oxygen species(ROS)in the injured skin may impede the wound repair and skin regeneration.Herein,we develop an injectable self-healing ceria-based nanocomposite hydrogel with ROS-scavenging activity ...Excessive reactive oxygen species(ROS)in the injured skin may impede the wound repair and skin regeneration.Herein,we develop an injectable self-healing ceria-based nanocomposite hydrogel with ROS-scavenging activity to accelerate wound healing.The nanocomposite hydrogels were successfully prepared by coating cerium oxide nanorods with polyethylenimine and crosslinked with benzaldehyde-terminated F127(F127-CHO)through the dynamic Schiff-base reaction(FVEC hydrogel).The results showed that the FVEC hydrogel possessed the good thermosensitivity,injectability,self-healing ability and ROS scavenging activity.The subcutaneous implantation experiments in mice confirmed that FVEC hydrogels are biocompatible and biodegradable in vivo.The fullthickness skin wound studies showed that FVEC hydrogel could significantly enhance the wound healing and epithelium regeneration with the formation of hair follicle and adipocyte tissue.This work provides a new strategy for the development of multifunctional Cebased nanocomposite hydrogel for full-thickness skin wound healing and regeneration.展开更多
Current treatments for full-thickness skin injuries are still unsatisfactory due to the lack of hierarchically stimulated dressings that can integrate the rapid hemostasis,inflammation regulation,and skin tissue remod...Current treatments for full-thickness skin injuries are still unsatisfactory due to the lack of hierarchically stimulated dressings that can integrate the rapid hemostasis,inflammation regulation,and skin tissue remodeling into the one system instead of single-stage boosting.In this work,a multilayer-structured bioactive glass nanopowder(BGN@PTE)is developed by coating the poly-tannic acid andε-polylysine onto the BGN via facile layer-by-layer assembly as an integrative and multilevel dressing for the sequential management of wounds.In comparison to BGN and poly-tannic acid coated BGN,BGN@PTE exhibited the better hemostatic performance because of its multiple dependent approaches to induce the platelet adhesion/activation,red blood cells(RBCs)aggregation and fibrin network formation.Simultaneously,the bioactive ions from BGN facilitate the regulation of the inflammatory response while the poly-tannic acid and antibacterialε-polylysine prevent the wound infection,promoting the wound healing during the inflammatory stage.In addition,BGN@PTE can serve as a reactive oxygen species scavenger,alleviate the oxidation stress in wound injury,induce the cell migration and angiogenesis,and promote the proliferation stage of wound repair.Therefore,BGN@PTE demonstrated the significantly higher wound repair capacity than the commercial bioglass dressing Dermlin™.This multifunctional BGN@PTE is a potentially valuable dressing for full-thickness wound management and may be expected to extend to the other wounds therapy.展开更多
'Bainong 3217 × Mardler' BC5F4 wheat line at the initial stage of inoculation with powdery mildew pathogen (Erysiphe graminis DC) was used to construct a suppression subtractive hybridization (SSH) cDNA l...'Bainong 3217 × Mardler' BC5F4 wheat line at the initial stage of inoculation with powdery mildew pathogen (Erysiphe graminis DC) was used to construct a suppression subtractive hybridization (SSH) cDNA library. Totally 760 ESTs were obtained through sequencing. Similarity analysis of ESTs based on BLASTn and BLASTx with the sequences in GenBank, in combination with macroarray differential screening, revealed that 199 ESTs of 65 kinds were known to be functionally disease resistance related. Based on the gene expression profiling in the present study, it is postulated that salicylic acid (SA) and MAP-related signal transduction pathways were involved in powdery mildew resistance in wheat. System acquired resistance genes were predominant in terms of kinds and quantity. With the initiation of cell defense reaction, the genes conferring anti-oxidation substances were largely expressed and thus cell protection mechanism was activated. Much evidence revealed that phenylpropanes metabolic pathway was展开更多
There is no doubt that COVID-19 outbreak is currently the biggest public health threat,which has caused catastrophic con sequences in many countries and regions.As host immunity is key to fighting against virus infect...There is no doubt that COVID-19 outbreak is currently the biggest public health threat,which has caused catastrophic con sequences in many countries and regions.As host immunity is key to fighting against virus infection,it is important to characterize the immunologic changes in the COVID-19 patients,and to explore potential therapeutic candidates.The most efficient ways to end this pandemic are to vaccinate the susceptible population,and to use specific drugs,such as monoclonal antibodies against the viral spike protein(S protein),to treat the affected individuals.Several promising neutralizing antibodies have recently been reported(Cao et al.,2020;Lv et al.,2020).展开更多
基金Supported by the National and Zhejiang Provincial Administration of Traditional Chinese Medicine Co-construction Project,No.GZYZJ-KJ-24080the Medical Science and Technology Project of Zhejiang Province,No.2024XY228.
文摘BACKGROUND Metabolic-associated fatty liver disease(MAFLD)is characterized by lipid accumulation in hepatocytes and is closely associated with oxidative stress.Increasing clinical evidence indicates that MAFLD is linked to bone metabolic disorders,including osteoporosis.Recent studies indicate that the expression profiles of liver circular RNAs(circRNAs)are altered in MAFLD.However,the effects of these changes on bone metabolism remain poorly understood.AIM To investigate the effects and mechanism of differently expressed circRNAs secreted by the liver on osteogenic differentiation in MAFLD.METHODS RNA sequencing was performed to identify highly expressed circRNAs in the liver,validated by quantitative real-time reverse transcription polymerase chain reaction,and localized using fluorescence in situ hybridization(FISH).A mouse model induced by a high-fat diet was used to simulate MAFLD.RESULTS CircSOD2 was significantly upregulated in liver tissues and primary hepatocytes from subjects with MAFLD.CircSOD2 was induced by oxidative stress and attenuated by antioxidants in the mouse model.In addition,circSOD2 was delivered from hepatocytes to bone marrow mesenchymal stem cells(BMSCs).Furthermore,circSOD2 inhibited the osteogenic differentiation of BMSCs and in vivo bone formation by sponging miR-29b.Moreover,miR-29b inhibition reversed the stimulatory effect of circSOD2 silencing on osteogenic differentiation of BMSCs and in vivo bone formation.Mechanistically,the interaction between circSOD2 and miR-29b confirmed through a luciferase reporter assay and the co-localization in the cytoplasm evidenced by FISH indicated that circSOD2 acted as a sponge for miR-29b.CONCLUSION This study provides a novel mechanism underlying the liver-bone crosstalk,demonstrating that circSOD2 upregulation in hepatocytes,induced by oxidative stress,inhibits osteogenic differentiation of BMSCs by sponging miR-29b.These findings offer a better understanding of the relationship between MAFLD and osteoporosis.
基金Supported by the National Natural Science Foundation of China,No.82100639,No.82200630,and No.81970526Postdoctoral Scientific Research Foundation of Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine,No.202401023+3 种基金Clinical Research Program of Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine,No.JYLJ202124Shanghai Municipal Commission of Health and Family Planning,No.20244Y0195 and No.20234Y0132the Fundamental Research Program Funding of Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine,No.JYZZ162Science Foundation of Xinjiang Uygur Natural Autonomous Region,No.2022D01F17.
文摘BACKGROUND Liver cirrhosis and portal hypertension(PHT)can lead to lymphatic abnormalities and coagulation dysfunction.Because lymphangiogenesis may relieve liver cirrhosis and PHT,the present study investigated the gene expression alterations in the lymphatic system and the effectiveness of platelet-mediated lymphangiogenesis in improving liver cirrhosis and PHT.AIM To investigate the role of lymphangiogenesis in preclinical PHT models.METHODS Immunohistochemistry and transcriptome sequencing of bile duct ligation(BDL)and control lymphatic samples were conducted to reveal the indicated signaling pathways.Functional enrichment analyses were performed on the differentially expressed genes and hub genes.Adenoviral infection of vascular endothelial growth factor C(VEGF-C),plateletrich plasma(PRP),and VEGF3 receptor(VEGFR)inhibitor MAZ-51 was used as an intervention for the lymphatic system in PHT models.Histology,hemodynamic tests and western blot analyses were performed to demonstrate the effects of lymphatic intervention in PHT patients.RESULTS Lymphangiogenesis was increased in the BDL rat model.Transcriptome sequencing analysis of the extrahepatic lymphatic system revealed its close association with platelet adherence,aggregation,and activation.The role of PHT in the rat model was investigated by activating(PRP)and inhibiting(MAZ-51)the lymphatic system.PRP promoted lymphangiogenesis,which increased lymphatic drainage,alleviated portal pressure,reduced liver fibrosis,inhibited inflammation,inhibited angiogenesis,and suppressed mesenteric artery remodeling.MAZ-51 reversed the above improvements.CONCLUSION Via VEGF-C/VEGFR-3,platelets impede fibrosis,angiogenesis,and mesenteric artery remodeling,ultimately alleviating PHT.Thus,platelet intervention is a therapeutic approach for cirrhosis and PHT.
基金funding from the National Natural Science Foundation of China(nos.52307028,52301192,and 52377026)State Key Laboratory of Electrical Insulation and Power Equipment Open Project(no.EIPE22210)+4 种基金Shaanxi Provincial Science and Technology Department Youth Foundation(no.2022JQ-300)Shaanxi Province Industry-University-Research Collaborative Innovation Plan(no.2023YFBT-45-02)Taishan Scholars and Young Experts Program of Shandong Province(no.tsqn202103057)the Qingchuang Talents Induction Program of Shandong Higher Education Institution(Research and Innovation Team of Structural-Functional Polymer Composites)Special Financial of Shandong Province(Structural Design of High-efficiency Electromagnetic Wave-absorbing Composite Materials and Construction of Shandong Provincial Talent Teams)。
文摘The introduction of highly polarized flexible segments into polymer molecular chains is an effective means to improve the dielectric constant and mechanical flexibility of polymers,which is important for improving the energy storage characteristics and applicability for the roll-to-roll process of metallic film capacitors.However,the introduction of flexible segments may adversely affect the heat resistance of polymer materials,which limits their application at high working temperatures.In this work,we have prepared a novel epoxy film with excellent comprehensive properties by introducing rigid phenyl and flexible etherified methylene side chains.For instance,the optimal epoxy film showed an energy storage density of 7.06 J cm^(-3)and 85%charge-discharge efficiency at room temperature and 420 kV mm^(-1).At 200 kV mm^(-1)and 110°C,a working condition for the application of the electric vehicle,the prepared film still showed an energy storage density of 1.5 J cm^(-3)and charge-discharge efficiency of 86%,which is 3 times that of BOPP film.This work provides an idea for material designs of high-performance polymer film capacitors.
基金supported by the Key Program(U20A20235)funded by the National Natural Science Foundation of Chinathe National Natural Science Foundation of China(52171127,51974242)+3 种基金the Natural Science Basic Research Program of Shaanxi(2023-JC-QN-0595)the Regional Innovation Capability Guidance Program of Shaanxi(2022QFY10-06)the Key R&D Program of Xianyang Science and Technology Bureau(2021ZDYF-GY-0029)the Program of Xi’an Science and Technology Bureau(23GXFW0066)。
文摘Polymer-based composite electrolytes composed of three-dimensional Li_(6.4)La_(3)Zr_(2)Al_(0.2)O_(12)(3D-LLZAO)have attracted increasing attention due to their continuous ion conduction and satisfactory mechanical properties.However,the organic/inorganic interface is incompatible,resulting in slow lithium-ion transport at the interface.Therefore,the compatibility of organic/inorganic interface is an urgent problem to be solved.Inspired by the concept of“gecko eaves”,polymer-based composite solid electrolytes with dense interface structures were designed.The bridging of organic/inorganic interfaces was established by introducing silane coupling agent(3-chloropropyl)trimethoxysilane(CTMS)into the PEO-3D-LLZAO(PL)electrolyte.The in-situ coupling reaction improves the interface affinity,strengthens the organic/inorganic interaction,reduces the interface resistance,and thus achieves an efficient interface ion transport network.The prepared PEO-3D-LLZAO-CTMS(PLC)electrolyte exhibits enhanced ionic conductivity of 6.04×10^(-4)S cm^(-1)and high ion migration number(0.61)at 60℃and broadens the electrochemical window(5.1 V).At the same time,the PLC electrolyte has good thermal stability and high mechanical properties.Moreover,the Li Fe PO_(4)|PLC|Li battery has excellent rate performance and cycling stability with a capacity decay rate of 2.2%after 100 cycles at 60℃and 0.1 C.These advantages of PLC membranes indicate that this design approach is indeed practical,and the in-situ coupling method provides a new approach to address interface compatibility issues.
基金supported by the Major Science and Technology Program for Water Pollution Control and Treatment of China (Nos. 2017ZX07106003-002 and 2017ZX07102004-002)
文摘The changes of protein secondary structures in the extracellular polymeric substances(EPS) extracted from activated sludge by four different methods were studied by analyzing the amide I region(1700–1600 cm-1) of the Fourier transform infrared spectra and model protein test. The results showed the molecular weight distribution of organic matter extracted by centrifugation, heating and cation exchange resin(CER) was similar, while the EPS extracted by centrifugation(Control) and CER had similar fluorescent organic matter. The protein secondary structures of extracted EPS by the four methods were different. The similarities of protein secondary structures between the EPS extracted by CER with the Control were the highest among the four extracted EPS. Although the EPS yield extracted by formaldehyde + NaOH method were the highest, its protein secondary structures had the lowest similarity with those extracted by the Control. Additionally, the effects of centrifugation and CER extraction on the secondary structures of bovine serum albumin were also lower than that of other extraction processes. CER enables the second maximum extraction of EPS and maximum retention of the original secondary structure of proteins.
文摘Quiescent state has been observed in stem cells(SCs),including in adult SCs and in cancer SCs(CSCs).Quiescent status of SCs contributes to SC self-renewal and conduces to averting SC death from harsh external stimuli.In this review,we provide an overview of intrinsic mechanisms and extrinsic factors that regulate adult SC quiescence.The intrinsic mechanisms discussed here include the cell cycle,mitogenic signaling,Notch signaling,epigenetic modification,and metabolism and transcriptional regulation,while the extrinsic factors summarized here include microenvironment cells,extracellular factors,and immune response and inflammation in microenvironment.Quiescent state of CSCs has been known to contribute immensely to therapeutic resistance in multiple cancers.The characteristics and the regulation mechanisms of quiescent CSCs are discussed in detail.Importantly,we also outline the recent advances and controversies in therapeutic strategies targeting CSC quiescence.
基金supported by the grants from the National Eleveth-Five-Year Key Task Project of China(No.2006BA102A01)the National "863" High Tech R & D Program of China(No.2006AA02A401)China-Sweden International Scientific and Technological Cooperative Project (No.09ZCZDSF04100)
文摘Objective： A number of studies have reported the association of ＂XPA＂, ＂XPC＂, ＂XPD/ERCC2＂ gene polymorphisms with lung cancer risk. However, the results were conflict. To clarify the impact of polymorphisms of ＂XPA＂, ＂XPC＂, ＂XPD/ERCC2＂, on lung cancer risk, a meta-analysis was performed in this study. Methods： The electronic databases PubMed and Embase were retrieved for studies included in this meta-analysis by ＂XPA＂, ＂XPC＂, ＂XPD/ERCC2＂, ＂lung＂, ＂cancer/neoplasm/tumor/carcinoma＂, ＂polymorphism＂ （An upper date limit of October, 31, 2009）. A meta-analysis was performed to evaluate the relationship among XPA, XPC and XPD polymorphism and lung cancer risks. Results： A total of 31 publications retrieved from Pubmed and Embase included in this study. XPC A939C CC genotype increased lung cancer risk in total population （recessive genetic model： OR=1.23, 95% CI：1.05-1.44; homozygote comparison： OR=1.21,95%CI：1.02-1.43and CC vs. CA contrast： OR=1.25,95%CI：1.06-1.48）, except in Asians. XPD A751C, 751C allele and CC genotype also increased lung cancer risk in total population and in Caucasians （recessive genetic model： Total population： OR=1.20, 95%CI：1.07-1.35）. No significant correlation was found between XPD A751C and lung cancer risk in Asians and African Americans. XPD G312A AA genotype increased lung cancer risk in total population, in Asians and Caucasians（recessive genetic model： Total population： OR=1.20, 95%CI： 1.06-1.36）. No significant association was found between XPA G23A, XPC C499T, XPD C156A and lung cancer risk. Conclusion： Our results suggest that the polymorphisms in XPC and XPD involve in lung cancer risks. XPA polymorphisms is less related to lung cancer risk.
基金National Natural Science Foundation of China(42406182)the China Postdoctoral Science Foundation Funded Project(GZC20232979)+4 种基金the National Natural Science Foundation of China(62205091)the China Postdoctoral Science Foundation Funded Project(2022M710983)HeiLongJiang Postdoctoral Foundation(LBH-Z22201)Supported by the Fundamental Research Foundation for Universities of Heilongjiang Province:2022-KYYWF-0121the China Postdoctoral Science Foundation Funded Project(2023TQ0369).
文摘Automatic mode-locking techniques,the integration of intelligent technologies with nonlinear optics offers the promise of on-demand intelligent control,potentially overcoming the inherent limitations of traditional ultrafast pulse generation that have predominantly suffered from the instability and suboptimality of open-loop manual tuning.The advancements in intelligent algorithm-driven automatic mode-locking techniques primarily are explored in this review,which also revisits the fundamental principles of nonlinear optical absorption,and examines the evolution and categorization of conventional mode-locking techniques.The convergence of ultrafast pulse nonlinear interactions with intelligent technologies has intricately expanded the scope of ultrafast photonics,unveiling considerable potential for innovation and catalyzing new waves of research breakthroughs in ultrafast photonics and nonlinear optics characters.
基金supported by a Ningxia Reproductive Disease Clinical Medical Research Center Project(2023-LCYX003)Ningxia Hui Autonomous Region Natural Science Foundation Project(2022AAC03748)+3 种基金Ningxia Hui Autonomous Region Natural Science Foundation Project(2021AAC03523)Yinchuan Science and Technology Innovation Project(2023SF25)Basic research project of Yinchuan Maternal and Child Health Hospital(2022NYFYCX05)Basic research project of Yinchuan Maternal and Child Health Hospital(2023NYFYCX01).
文摘Triple-negative breast cancer(TNBC)is an inherently heterogeneous malignancy at the biological and clinical levels,occupying around 15%of diagnosed breast cancer cases and being the most lethal breast cancer subtype to combat.1 Anoikis is a programmed cell death that occurs when cells detach from the correct extracellular matrix,thereby destroying integrin connections.2 However,the role of anoikis expression pattern and prognosis in TNBC is still unrevealed.Here,TNBC presented three heterogeneous phenotypes in anoikis,and each subtype owned unique molecular,and clinical characteristics as well as diverse responses to chemotherapy,targeted therapies,and immune checkpoint blockade.The anoikis-based scoring system can accurately estimate patient survival,which was remarkably superior to well-established clinical parameters.
基金supported by the Ningxia Reproductive Disease Clinical Medical Research Center Project(No.2023LCYX003)Ningxia Hui Autonomous Region Natural Science Foundation Project(2022AAC03748)+3 种基金Ningxia Hui Autonomous Region Natural Science Foundation Project(No.2021AAC03523)Basic research project of Yinchuan Maternal and Child Health Hospital(No.2022NYFYCX05)Yinchuan Science and Technology Innovation Project(No.2023SF25)Basic research project of Yinchuan Maternal and Child Health Hospital(No.2023NYFYCX01)(all China).
文摘Breast cancer(BRCA)is the dominating form of cancer affecting women,with an estimated 2.3 million newly diagnosed cases and 685,000 deaths in 2020.1 An emerging study has proposed a novel metabolism-related regulated cell'death modality called disulfidptosis induced by abnormal_accumulation_of intracellular disulfides in SLC7A11high tumor cells.2 However,research on disulfidptosis remains in its infancy.The study presents for the first time a comprehensive investigation on disulfidptosis in BRCA.
基金supported by the National Natural Science Foundation of China (82272057 and 32301160)。
文摘Acidity-activatable magnetic resonance imaging(MRI)nanoprobes offer great potential for in vivo cancer imaging by targeting the acidic tumor microenvironment(TME).However,their effectiveness is limited by the delayed response at tumor sites and uncontrollable background noise,compromising imaging accuracy and reliability.Herein,an acidic TME-responsive nanoprobe,SPIO@ZIF-8@Gd(SZG),with dually activatable T_(1) and T_(2) MR signals is shown for acidity-selective contrast enhancement in a rapid response manner.It shows decreased T_(1) and T_(2) contrast intensity in normal physiological conditions.Once targeting acidic TME,the zeolitic imidazolate framework-8(ZIF-8)layer undergoes instantaneous decomposition,releasing Gd^(3+)(T_(1)-weighted),and exposing the inner SPIO(T_(2)-weighted)core,thereby sequentially recovering the signals.Compared to previously reported T_(1)-T_(2) nanoprobes,SZG demonstrates noticeable“dual activation”after just 30 min and reaches its peak 4 h after acid incubation.Additionally,it shows an excellent“acidity correlation”between relaxation times and pH values.When the SZG nanoprobe is used combined with“dual-contrast enhanced subtraction(DESI)”,the contrast difference between diseased and normal tissue can be increased by 10 times,which is significantly higher than traditional single-mode T_(1)/T_(2) contrast agents.Collectively,these findings demonstrate a rapid imaging strategy of dual-activation MR imaging of the acidic TME and simultaneous background suppression,thus paving the way for precise tumor malignancy differentiation,early tumor detection,and accurate tumor grading.
基金This study was supported by grants from the National Natural Science Foundation of China(Grant No.82172344 to J.Z.)the Natural Science Foundation of Zhejiang Province(Grant No.LY21H160039 to J.Z.)+1 种基金the funding of Medical Science and Technology Project of Zhejiang Province(Grant No.2022RC174 to J.Z.)the Natural Science Foundation of Zhejiang Province(Grant No.LGF21H030010 to B.L.).
文摘Ductal carcinoma in situ(DCIS)represents pre-invasive breast carcinoma.In untreated cases,25-60%DCIS progress to invasive ductal carcinoma(IDC).The challenge lies in distinguishing between non-progressive and progressive DCIS,often resulting in over-or under-treatment in many cases.With increasing screen-detected DCIS in these years,the nature of DCIS has aroused worldwide attention.A deeper understanding of the biological nature of DCIS and the molecular journey of the DCIS-IDC transition is crucial for more effective clinical management.Here,we reviewed the key signaling pathways in breast cancer that may contribute to DCIS initiation and progression.We also explored the molecular features of DCIS and IDC,shedding light on the progression of DCIS through both inherent changes within tumor cells and alterations in the tumor microenvironment.In addition,valuable research tools utilized in studying DCIS including preclinical models and newer advanced technologies such as single-cell sequencing,spatial transcriptomics and artificial intelligence,have been systematically summarized.Further,we thoroughly discussed the clinical advancements in DCIS and IDC,including prognostic biomarkers and clinical managements,with the aim of facilitating more personalized treatment strategies in the future.Research on DCIS has already yielded significant insights into breast carcinogenesis and will continue to pave the way for practical clinical applications.
基金This study was supported by the funding of the National Natural Science Foundation of China(Grant No.82172344,81702866,Jiaojiao Zhou)Natural Science Foundation of Zhejiang Province(Grant No.LY21H160039,Jiaojiao Zhou)+1 种基金Fundamental Research Funds for the Central Universities(Grant No.2021FZJD009,Jiaojiao Zhou)the National Natural Science Foundation of China(Grant No.82072900,Yiding Chen).
文摘Circulating tumor cells(CTCs)are tumor cells that have sloughed off the primary tumor and extravasate into and circulate in the blood.Understanding of the metastatic cascade of CTCs has tremendous potential for the identification of targets against cancer metastasis.Detecting these very rare CTCs among the massive blood cells is challenging.However,emerging technologies for CTCs detection have profoundly contributed to deepening investigation into the biology of CTCs and have facilitated their clinical application.Current technologies for the detection of CTCs are summarized herein,together with their advantages and disadvantages.The detection of CTCs is usually dependent on molecular markers,with the epithelial cell adhesion molecule being the most widely used,although molecular markers vary between different types of cancer.Properties associated with epithelial-tomesenchymal transition and stemness have been identified in CTCs,indicating their increased metastatic capacity.Only a small proportion of CTCs can survive and eventually initiate metastases,suggesting that an interaction and modulation between CTCs and the hostile blood microenvironment is essential for CTC metastasis.Single-cell sequencing of CTCs has been extensively investigated,and has enabled researchers to reveal the genome and transcriptome of CTCs.Herein,we also review the clinical applications of CTCs,especially for monitoring response to cancer treatment and in evaluating prognosis.Hence,CTCs have and will continue to contribute to providing significant insights into metastatic processes and will open new avenues for useful clinical applications.
基金This research was financially supported by the National Natural Science Foundation of China(Nos.51673214 and 61804185)the National Key Research and Development Program of China(No.2017YFA0206600)+1 种基金the Natural Science Foundation of Hunan Province(No.2019JJ50804)the Free Exploration and Innovation Project of Central South University(No.2019zzts427).
文摘Flexible strain sensors exhibit outstanding advantages in terms of sensitivity and stability by detecting changes in physical signals.It can be easily attached to human skin and clothed to achieve monitoring of human motion and health.However,general sensing material shows low stretchability and cannot respond to signals under large deformation.In this work,a highly stretchable polymer composite was developed by adding small amount(0.17 wt.%)of silver nanowires(AgNWs)in stretchable conductive polymer materials.The conductivity of polymer/AgNWs composite is 1.3 S/m with the stretchability up to 500%.The stretchable strain sensor based on the polymer/AgNWs composite can respond to strain signals in real time,even for 1%strain response,and shows excellent stability over 1,000 loading/unloading cycles.Moreover,the strain sensor can be attached to human skin and clothed to monitor joints,throat and pulse of the human body.The human body electrocardiogram(ECG)signal was detected successfully with the polymer/AgNWs electrode,which is comparable to the signal obtained by the commercial electrode.Overall,the sensors enable monitoring of human movement and health.These advantages make it a potential application in wearable devices and electronic skin.
基金supported by National Natural Science Foundation of China(Grant No.51872224)Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research,College of Stomatology,Xi'an Jiaotong University(Grant No.2018LHMKFKT004)+2 种基金Special Guidance Funds for the Construction of World-class Universities(disciplines)and Characteristic Development in Central Universities(grant No.PY3A078)the Fundamental Research Funds for the Central Universities(grant No.xzy022019050)Wenzhou Science&Technology Bureau project(grant No.ZY2019003).
文摘The efficient cutaneous wound healing accompanied with the enhanced skin appendage regeneration is still a challenge.The bacterial infection and excessive/prolonged inflammation inhibit wound healing process and result in the scar formation.Herein,we reported an anti-inflammatory polycitrate-polyethyleneimine-Ibuprofen(PCEI)and multifunctional PCEI-based F127-ε-polypeptide-alginic(FEA)dressing(FEA-PCEI)for accelerating wound healing and hair follicle neogenesis.PCEI showed the excellent anti-inflammation function through stimulating macrophage towards anti-inflammatory M2 subtype polarization.The FEA-PCEI dressing showed the temperature-response gelation,injectability,robust antibacterial activity,light-damage-resistant,homeostasis ability,and good cytocompatibility.The optimized dosage of FEA-PCEI dressing could significantly accelerate wound healing with anti-infection ability,reduce the scar formation,and promote the hair follicle neogenesis.This study provided a wound-repairing strategy through regulating the phenotype of immune cells by the designing bioactive multifunctional biomaterials.
基金supported by the Scientific and Technological Project of Henan Province(182102310300)Key Research Projects of Colleges and Universities in Henan Province(18A150028).
文摘Excessive reactive oxygen species(ROS)in the injured skin may impede the wound repair and skin regeneration.Herein,we develop an injectable self-healing ceria-based nanocomposite hydrogel with ROS-scavenging activity to accelerate wound healing.The nanocomposite hydrogels were successfully prepared by coating cerium oxide nanorods with polyethylenimine and crosslinked with benzaldehyde-terminated F127(F127-CHO)through the dynamic Schiff-base reaction(FVEC hydrogel).The results showed that the FVEC hydrogel possessed the good thermosensitivity,injectability,self-healing ability and ROS scavenging activity.The subcutaneous implantation experiments in mice confirmed that FVEC hydrogels are biocompatible and biodegradable in vivo.The fullthickness skin wound studies showed that FVEC hydrogel could significantly enhance the wound healing and epithelium regeneration with the formation of hair follicle and adipocyte tissue.This work provides a new strategy for the development of multifunctional Cebased nanocomposite hydrogel for full-thickness skin wound healing and regeneration.
基金This work was supported by the Special Support Program for High Level Talents of Shaanxi Province of China,the key R&D plan of Shaanxi Province of China(grant No.2021GXLH-Z-052)State Key Laboratory for Manufacturing Systems Engineering of China(grant No.sklms2021006)Young Talent Support Plan of Xi’an Jiaotong University of China(grant No.QY6J003).
文摘Current treatments for full-thickness skin injuries are still unsatisfactory due to the lack of hierarchically stimulated dressings that can integrate the rapid hemostasis,inflammation regulation,and skin tissue remodeling into the one system instead of single-stage boosting.In this work,a multilayer-structured bioactive glass nanopowder(BGN@PTE)is developed by coating the poly-tannic acid andε-polylysine onto the BGN via facile layer-by-layer assembly as an integrative and multilevel dressing for the sequential management of wounds.In comparison to BGN and poly-tannic acid coated BGN,BGN@PTE exhibited the better hemostatic performance because of its multiple dependent approaches to induce the platelet adhesion/activation,red blood cells(RBCs)aggregation and fibrin network formation.Simultaneously,the bioactive ions from BGN facilitate the regulation of the inflammatory response while the poly-tannic acid and antibacterialε-polylysine prevent the wound infection,promoting the wound healing during the inflammatory stage.In addition,BGN@PTE can serve as a reactive oxygen species scavenger,alleviate the oxidation stress in wound injury,induce the cell migration and angiogenesis,and promote the proliferation stage of wound repair.Therefore,BGN@PTE demonstrated the significantly higher wound repair capacity than the commercial bioglass dressing Dermlin™.This multifunctional BGN@PTE is a potentially valuable dressing for full-thickness wound management and may be expected to extend to the other wounds therapy.
基金This work was supported by the National "973" Program (Grant No. G1998010200) the National Natural Science Foundation of China (Grant No. 39980029).
文摘'Bainong 3217 × Mardler' BC5F4 wheat line at the initial stage of inoculation with powdery mildew pathogen (Erysiphe graminis DC) was used to construct a suppression subtractive hybridization (SSH) cDNA library. Totally 760 ESTs were obtained through sequencing. Similarity analysis of ESTs based on BLASTn and BLASTx with the sequences in GenBank, in combination with macroarray differential screening, revealed that 199 ESTs of 65 kinds were known to be functionally disease resistance related. Based on the gene expression profiling in the present study, it is postulated that salicylic acid (SA) and MAP-related signal transduction pathways were involved in powdery mildew resistance in wheat. System acquired resistance genes were predominant in terms of kinds and quantity. With the initiation of cell defense reaction, the genes conferring anti-oxidation substances were largely expressed and thus cell protection mechanism was activated. Much evidence revealed that phenylpropanes metabolic pathway was
基金This work was funded by the National Natural Science Foundation of China(Nos.61822108,62041102 and 62032007 to Q.J.)the Emergency Research Project for COVID-19 of Harbin Institute of Technology(No.2020-001 to Q.J.)the Scientific Research Project Approved by Heilongjiang Provincial Health Committee(No.2019-253 to J.L.).
文摘There is no doubt that COVID-19 outbreak is currently the biggest public health threat,which has caused catastrophic con sequences in many countries and regions.As host immunity is key to fighting against virus infection,it is important to characterize the immunologic changes in the COVID-19 patients,and to explore potential therapeutic candidates.The most efficient ways to end this pandemic are to vaccinate the susceptible population,and to use specific drugs,such as monoclonal antibodies against the viral spike protein(S protein),to treat the affected individuals.Several promising neutralizing antibodies have recently been reported(Cao et al.,2020;Lv et al.,2020).
