[Objectives]The paper was to establish the quality standard of Yi herb medicine Millettia dielsiana.[Methods]Yi herb medicine M.dielsiana was qualitatively identified by microscopic identification and thin layer ident...[Objectives]The paper was to establish the quality standard of Yi herb medicine Millettia dielsiana.[Methods]Yi herb medicine M.dielsiana was qualitatively identified by microscopic identification and thin layer identification.The content of total isoflavones was determined by UV-Vis spectrophotometry,and the contents of moisture,total ash,acid-insoluble ash and extract were checked according to the method introduced in Chinese Pharmacopoeia(2020 edition).[Results]The microscopic identification features,including stone cells,fiber bundles,calcium oxalate square crystals,starch granules,pigment blocks,ducts,etc.,were distinct.A TLC method for identification of M.dielsiana was established.The contents of water,total ash,acid-insoluble ash and extract should be no more than 13%,no more than 7%,no more than 0.5%and no less than 6%in 10 batches of samples,respectively.The total isoflavones of M.dielsiana based on genistein(C_(15)H_(10)O_(5)) should be no less than 0.25%.The linear relationship of genistein was good in the range of 0.002-0.007 mg/mL(R^(2)=0.9996);the average recovery was 97.01%and the RSD was 2.62%.[Conclusions]The method is simple,accurate and well-reproducible,and can be used for the quality control of Yi herb medicine M.dielsiana.展开更多
Atopic dermatitis(AD)is a prevalent inflammatory skin disorder in which patients experience recurrent eczematous lesions and intense itching.The colonization of Staphylococcus aureus(S.aureus)is correlated with the se...Atopic dermatitis(AD)is a prevalent inflammatory skin disorder in which patients experience recurrent eczematous lesions and intense itching.The colonization of Staphylococcus aureus(S.aureus)is correlated with the severity of the disease,but its role in AD development remains elusive.Using single-cell RNA sequencing,we uncovered that keratinocytes activate a distinct immune response characterized by induction of Il24 when exposed to methicillin-resistant S.aureus(MRSA).Further experiments using animal models showed that the administration of recombinant IL-24 protein worsened AD-like pathology.Genetic ablation of Il24 or the receptor Il20rb in keratinocytes alleviated allergic inflammation and atopic march.Mechanistically,IL-24 acted through its heterodimeric receptors on keratinocytes and augmented the production of IL-33,which in turn aggravated type 2 immunity and AD-like skin conditions.Overall,these findings establish IL-24 as a critical factor for onset and progression of AD and a compelling therapeutic target.展开更多
基金Supported by National Key Research and Development Program of China(2018YFC1706101)Science and Technology Program of Sichuan Province(Key Research and Development Project)(2021YFS0043)+2 种基金Standard Improvement of Traditional Chinese Medicine(Ethnic Medicine)of Sichuan Provincial Drug Administration(510201202102305)2022 Leading Talents Support Program of National Ethnic Affairs CommissionUniversity-level Innovation Team Special Project of Basic Research Funds for Central Universities and Colleges(ZYN2022067)。
文摘[Objectives]The paper was to establish the quality standard of Yi herb medicine Millettia dielsiana.[Methods]Yi herb medicine M.dielsiana was qualitatively identified by microscopic identification and thin layer identification.The content of total isoflavones was determined by UV-Vis spectrophotometry,and the contents of moisture,total ash,acid-insoluble ash and extract were checked according to the method introduced in Chinese Pharmacopoeia(2020 edition).[Results]The microscopic identification features,including stone cells,fiber bundles,calcium oxalate square crystals,starch granules,pigment blocks,ducts,etc.,were distinct.A TLC method for identification of M.dielsiana was established.The contents of water,total ash,acid-insoluble ash and extract should be no more than 13%,no more than 7%,no more than 0.5%and no less than 6%in 10 batches of samples,respectively.The total isoflavones of M.dielsiana based on genistein(C_(15)H_(10)O_(5)) should be no less than 0.25%.The linear relationship of genistein was good in the range of 0.002-0.007 mg/mL(R^(2)=0.9996);the average recovery was 97.01%and the RSD was 2.62%.[Conclusions]The method is simple,accurate and well-reproducible,and can be used for the quality control of Yi herb medicine M.dielsiana.
基金supported by the National Key R&D Program of China(2023YFC2306300)the National Natural Science Foundation of China(Grant Nos.32225019,92357304,and 32394003)supported by the Center for Life Sciences,the Institute for Immunology,and School of Basic Medical Sciences at Tsinghua University.
文摘Atopic dermatitis(AD)is a prevalent inflammatory skin disorder in which patients experience recurrent eczematous lesions and intense itching.The colonization of Staphylococcus aureus(S.aureus)is correlated with the severity of the disease,but its role in AD development remains elusive.Using single-cell RNA sequencing,we uncovered that keratinocytes activate a distinct immune response characterized by induction of Il24 when exposed to methicillin-resistant S.aureus(MRSA).Further experiments using animal models showed that the administration of recombinant IL-24 protein worsened AD-like pathology.Genetic ablation of Il24 or the receptor Il20rb in keratinocytes alleviated allergic inflammation and atopic march.Mechanistically,IL-24 acted through its heterodimeric receptors on keratinocytes and augmented the production of IL-33,which in turn aggravated type 2 immunity and AD-like skin conditions.Overall,these findings establish IL-24 as a critical factor for onset and progression of AD and a compelling therapeutic target.
