Background:The composition of the intestinal flora and the resulting metabolites af-fect patients'sleep after surgery.Methods:We intended to elucidate the mechanisms by which disordered intestinal flora modulate t...Background:The composition of the intestinal flora and the resulting metabolites af-fect patients'sleep after surgery.Methods:We intended to elucidate the mechanisms by which disordered intestinal flora modulate the pathophysiology of postoperative sleep disturbances in hosts.In this study,we explored the impacts of anesthesia,surgery,and postoperative sleep duration on the fecal microbiota and metabolites of individuals classified postpro-cedurally as poor sleepers(PS)and good sleepers(GS),as diagnosed by the bispec-tral index.We also performed fecal microbiota transplantation in pseudo-germ-free(PGF)rats and applied Western blotting,immunohistochemistry,and gut permeability analyses to identify the potential mechanism of its effect.Results:Research finding shows the PS group had significantly higher postopera-tive stool levels of the metabolites tryptophan and kynurenine than the GS group.PGF rats that received gut microbiota from PSs exhibited less rapid eye movement(REM)sleep than those that received GS microbiota(GS-PGF:11.4%±1.6%,PS-PGF:4.8%±2.0%,p<0.001).Measurement of 5-hydroxytryptophan(5-HTP)levels in the stool,serum,and prefrontal cortex(PFC)indicated that altered 5-HTP levels,includ-ing reduced levels in the PFC,caused sleep loss in PGF rats transplanted with PS gut flora.Through the brain-gut axis,the inactivity of tryptophan hydroxylase 1(TPH1)and TPH2 in the colon and PFC,respectively,caused a loss of REM sleep in PGF rats and decreased the 5-HTP level in the PFC.Conclusions:These findings indicate that postoperative gut dysbiosis and defective 5-HTP metabolism may cause postoperative sleep disturbances.Clinicians and sleep researchers may gain new insights from this study.展开更多
As a new interdisciplinary field,synthetic biology has led to valuable innovations in the fields of medicine,chemistry,agriculture,energy and environment.In this paper,we systematically review the development status o...As a new interdisciplinary field,synthetic biology has led to valuable innovations in the fields of medicine,chemistry,agriculture,energy and environment.In this paper,we systematically review the development status of global synthetic biology in the past six years,and make an in-depth benchmarking analysis of the field in China.With the aid of Scopus and SciVal,we analyze the scholarly output of synthetic biology in the world and individual countries,including publication distribution,popular journals and eminent institutions.Furthermore,the research focus and concepts,citation impact and collaborations are also examined using numerical index methods such as the field-weighted citation impact(FWCI)and relative activity index(RAI),showing the differences between data more intuitively.This study aims to offer a comprehensive understanding of the research status of synthetic biology in China and the world,offering a benchmarked overview of the results as a reference to guide the development of this field in the future.展开更多
基金supported by a grant from the National Natural Science Foundation of China(82171187).
文摘Background:The composition of the intestinal flora and the resulting metabolites af-fect patients'sleep after surgery.Methods:We intended to elucidate the mechanisms by which disordered intestinal flora modulate the pathophysiology of postoperative sleep disturbances in hosts.In this study,we explored the impacts of anesthesia,surgery,and postoperative sleep duration on the fecal microbiota and metabolites of individuals classified postpro-cedurally as poor sleepers(PS)and good sleepers(GS),as diagnosed by the bispec-tral index.We also performed fecal microbiota transplantation in pseudo-germ-free(PGF)rats and applied Western blotting,immunohistochemistry,and gut permeability analyses to identify the potential mechanism of its effect.Results:Research finding shows the PS group had significantly higher postopera-tive stool levels of the metabolites tryptophan and kynurenine than the GS group.PGF rats that received gut microbiota from PSs exhibited less rapid eye movement(REM)sleep than those that received GS microbiota(GS-PGF:11.4%±1.6%,PS-PGF:4.8%±2.0%,p<0.001).Measurement of 5-hydroxytryptophan(5-HTP)levels in the stool,serum,and prefrontal cortex(PFC)indicated that altered 5-HTP levels,includ-ing reduced levels in the PFC,caused sleep loss in PGF rats transplanted with PS gut flora.Through the brain-gut axis,the inactivity of tryptophan hydroxylase 1(TPH1)and TPH2 in the colon and PFC,respectively,caused a loss of REM sleep in PGF rats and decreased the 5-HTP level in the PFC.Conclusions:These findings indicate that postoperative gut dysbiosis and defective 5-HTP metabolism may cause postoperative sleep disturbances.Clinicians and sleep researchers may gain new insights from this study.
基金financially supported by the National Natural Science Foundation of China (21776209, 21621004 and 21776208)Ministry of Education of the People’s Republic of China Humanities and Social Sciences Youth Foundation (21YJCZH232)the Natural Science Foundation of Tianjin City (No. 19JCYBJC21100)
文摘As a new interdisciplinary field,synthetic biology has led to valuable innovations in the fields of medicine,chemistry,agriculture,energy and environment.In this paper,we systematically review the development status of global synthetic biology in the past six years,and make an in-depth benchmarking analysis of the field in China.With the aid of Scopus and SciVal,we analyze the scholarly output of synthetic biology in the world and individual countries,including publication distribution,popular journals and eminent institutions.Furthermore,the research focus and concepts,citation impact and collaborations are also examined using numerical index methods such as the field-weighted citation impact(FWCI)and relative activity index(RAI),showing the differences between data more intuitively.This study aims to offer a comprehensive understanding of the research status of synthetic biology in China and the world,offering a benchmarked overview of the results as a reference to guide the development of this field in the future.
