The surface velocity is one of the most important characteristics of glaciers.Monitoring and mapping glacier movements are of great significance for the studies of sea-level rise,glacier mass balance and dynamics,glob...The surface velocity is one of the most important characteristics of glaciers.Monitoring and mapping glacier movements are of great significance for the studies of sea-level rise,glacier mass balance and dynamics,global warming,and the management of freshwater resources.It is also essential for the early warnings of hazards caused by ice avalanches.SAR imaging geodesy has been developed for measuring glacier velocity,especially the pixel-offset tracking method.This paper introduces some basic concepts of glaciology and principles of various SAR imaging geodesy methods,with a detailed presentation about the developments in the applications of the pixel-offset tracking method.Finally,the challenges and future prospects of SAR imaging geodesy in glacier monitoring are discussed.展开更多
Three-dimensional(3D)hydrogel models play a crucial role in tissue engineering for promoting tissue regeneration.A biomimetic microchannel network system in the 3D hydrogel model is necessary for optimal cellular func...Three-dimensional(3D)hydrogel models play a crucial role in tissue engineering for promoting tissue regeneration.A biomimetic microchannel network system in the 3D hydrogel model is necessary for optimal cellular function.This report describes the preparation of a biomimetic hydrogel scaffold with an internal microchannel network,using electrospinning techniques and the sacrificial template method for 3D cell culture.Microchannels and cavities were created within the gelatin methacryloyl(GelMA)hydrogel by sacrificing polyvinyl alcohol(PVA)electrospun fibers(>10µm),resulting in the creation of microvessel-like channels.Mechanical characterizations,swelling properties,and biodegradation analysis were conducted to investigate the feasibility of a biomimetic microchannel network hydrogel scaffold for 3D cell culture applications.Compared to pure GelMA hydrogel,the hydrogel with microchannels promoted cell proliferation,adhesion,and endothelial tube formation.Moreover,the results confirmed that the biomimetic microchannel network scaffold had a major impact on the distribution and arrangement of human umbilical vein endothelial cells(HUVECs)and can enable the formation of artificial microvasculature by the culture of HUVECs and cell media perfusion.展开更多
Hypoxia-inducible factor(HIF)is a main heterodimeric transcription factor that regulates the cellular adaptive response to hypoxia by stimulating the transcription of a series of hypoxia-inducible genes.HIF is frequen...Hypoxia-inducible factor(HIF)is a main heterodimeric transcription factor that regulates the cellular adaptive response to hypoxia by stimulating the transcription of a series of hypoxia-inducible genes.HIF is frequently upregulated in solid tumors,and the overexpression of HIF can promote tumor progression or aggressiveness by blood vessel architecture and altering cellular metabolism.In this review,we focused on the pivotal role of HIF in tumor angiogenesis and energy metabolism.Furthermore,we also emphasized the possibility of HIF pathway as a potential therapeutic target in cancer.展开更多
The purpose of this study is to measure the expression of microRNA-4463 and microRNA-6087 between normal persons and patients with hepatocellular carcinoma(HCC),and to clarify the meaning of them in the prognosis eval...The purpose of this study is to measure the expression of microRNA-4463 and microRNA-6087 between normal persons and patients with hepatocellular carcinoma(HCC),and to clarify the meaning of them in the prognosis evaluation in HCC.Forty-five samples from healthy people and patients,who had been diagnosed with hepatocellular carcinoma before any treatment,were collected to study respectively.Real-time PCR was used to detect the expression of miRNA-4463 and miRNA-6087 in the serum of control group and hepatocellular carcinoma patients.The expression of miR-4463 in the serum of HCC patients was significantly higher than that in control group(P<0.05),and the expression level was independent of gender,tumor size,cell types,stages,alanine aminotransferase(ALT),aspartate aminotransferase(AST),total bilirubin(TBIL)and HBsAg status(P>0.05).But there was a significant difference of different level of AFP in HCC(P<0.05),and the difference between the group of AFP lower than 400 ug/l and the control group is statistically significant(P<0.05).Besides,the survival time had showed a significant difference at the high and low expression levels(P<0.05).But the expression level of miRNA-6087 was no difference in HCC and control group.The disorder of miRNA-4463 occurred in HCC,even the AFP level doesn’t rises.What’s more,patients who get the high level of miRNA-4463 seem to have a shorter survival time.And it contributes great to the prognostic evaluation.This is the first study to illustrate the potential significance of miRNA-4463 in the prognosis in HCC.展开更多
Skin tissue engineering with considerable skin regeneration capability is an urgent need for the wound site.The current challenge for researchers is to develop a bionic scaffold that imitates the extracellular matrix ...Skin tissue engineering with considerable skin regeneration capability is an urgent need for the wound site.The current challenge for researchers is to develop a bionic scaffold that imitates the extracellular matrix for the regeneration of the damaged regions.In our study,poly(L-lactide-co-caprolactone)(PLCL)was blended with polyurethane(PU)to obtain nanofibrous scaffolds via electrospinning.The electrospun fibers with 50%PLCL content had a certain number of intersections and jointing points,and exhibited significantly enhanced mechani-cal properties combined with suitable porosity.Moreover,cell activities demonstrated that PU/PLCL membranes had significantly biological advantages in enhanced growth of human skin fibroblasts with spreading morphology compared with PU membranes,indicating good cytocompatibility of composite scaffolds.These findings proved that PU/PLCL electrospun membranes have great potential in applications of skin tissue engineering.展开更多
The publisher regrets that ethical approval was not included in the published version.Upon request,the authors have provided the appropriate ethical approval statement,which states that the human skin fibroblasts used...The publisher regrets that ethical approval was not included in the published version.Upon request,the authors have provided the appropriate ethical approval statement,which states that the human skin fibroblasts used in this study were derived from normal skin tissues of patients after scar resection at Shanxi Bethune Hospital,and were obtained under ethical requirements.The experimental process strictly adhered to relevant ethical requirements and guidelines,and was granted approval by the ethics committee with protocol number SBQLL-2021-065 from Shanxi Bethune Hospital.展开更多
文摘The surface velocity is one of the most important characteristics of glaciers.Monitoring and mapping glacier movements are of great significance for the studies of sea-level rise,glacier mass balance and dynamics,global warming,and the management of freshwater resources.It is also essential for the early warnings of hazards caused by ice avalanches.SAR imaging geodesy has been developed for measuring glacier velocity,especially the pixel-offset tracking method.This paper introduces some basic concepts of glaciology and principles of various SAR imaging geodesy methods,with a detailed presentation about the developments in the applications of the pixel-offset tracking method.Finally,the challenges and future prospects of SAR imaging geodesy in glacier monitoring are discussed.
基金supported by the National Natural Science Foundation of China(No.31870934)the Natural Science Foundation for Young Scientists of Shanxi Province(No.202103021223100),China.
文摘Three-dimensional(3D)hydrogel models play a crucial role in tissue engineering for promoting tissue regeneration.A biomimetic microchannel network system in the 3D hydrogel model is necessary for optimal cellular function.This report describes the preparation of a biomimetic hydrogel scaffold with an internal microchannel network,using electrospinning techniques and the sacrificial template method for 3D cell culture.Microchannels and cavities were created within the gelatin methacryloyl(GelMA)hydrogel by sacrificing polyvinyl alcohol(PVA)electrospun fibers(>10µm),resulting in the creation of microvessel-like channels.Mechanical characterizations,swelling properties,and biodegradation analysis were conducted to investigate the feasibility of a biomimetic microchannel network hydrogel scaffold for 3D cell culture applications.Compared to pure GelMA hydrogel,the hydrogel with microchannels promoted cell proliferation,adhesion,and endothelial tube formation.Moreover,the results confirmed that the biomimetic microchannel network scaffold had a major impact on the distribution and arrangement of human umbilical vein endothelial cells(HUVECs)and can enable the formation of artificial microvasculature by the culture of HUVECs and cell media perfusion.
基金Grant sponsor:National Nature Science Foundation of China,Grant number:81272960Key Research Program from Science and Technology Department of Hunan Province China,Grant number:2013WK2010 and 2014SK2015+3 种基金The fund of Tianqing liver disease researchThis work was supported by National Nature Science Foundation of China(81272960)Key Research Program from Science and Technology Department of Hunan Province,China(2013WK2010 and 2014SK2015)Key Research Program from Ministry of human Resources and Social Security of the People’s Republic of China(2016)176.
文摘Hypoxia-inducible factor(HIF)is a main heterodimeric transcription factor that regulates the cellular adaptive response to hypoxia by stimulating the transcription of a series of hypoxia-inducible genes.HIF is frequently upregulated in solid tumors,and the overexpression of HIF can promote tumor progression or aggressiveness by blood vessel architecture and altering cellular metabolism.In this review,we focused on the pivotal role of HIF in tumor angiogenesis and energy metabolism.Furthermore,we also emphasized the possibility of HIF pathway as a potential therapeutic target in cancer.
基金National Nature Science Foundation of China,Grant number:81272960Key Research Program from Science and Technology Department of Hunan Province China,Grant number:2013WK2010 and 2014SK2015+1 种基金Key Research Program from Ministry of human Resources and Social Security of the People’s Republic of China,Grant number:(2016)176The fund of Tianqing liver disease research,Grant number:(TQGB20140155).
文摘The purpose of this study is to measure the expression of microRNA-4463 and microRNA-6087 between normal persons and patients with hepatocellular carcinoma(HCC),and to clarify the meaning of them in the prognosis evaluation in HCC.Forty-five samples from healthy people and patients,who had been diagnosed with hepatocellular carcinoma before any treatment,were collected to study respectively.Real-time PCR was used to detect the expression of miRNA-4463 and miRNA-6087 in the serum of control group and hepatocellular carcinoma patients.The expression of miR-4463 in the serum of HCC patients was significantly higher than that in control group(P<0.05),and the expression level was independent of gender,tumor size,cell types,stages,alanine aminotransferase(ALT),aspartate aminotransferase(AST),total bilirubin(TBIL)and HBsAg status(P>0.05).But there was a significant difference of different level of AFP in HCC(P<0.05),and the difference between the group of AFP lower than 400 ug/l and the control group is statistically significant(P<0.05).Besides,the survival time had showed a significant difference at the high and low expression levels(P<0.05).But the expression level of miRNA-6087 was no difference in HCC and control group.The disorder of miRNA-4463 occurred in HCC,even the AFP level doesn’t rises.What’s more,patients who get the high level of miRNA-4463 seem to have a shorter survival time.And it contributes great to the prognostic evaluation.This is the first study to illustrate the potential significance of miRNA-4463 in the prognosis in HCC.
基金funded by National Natural Science Foundation of China(No.31870934).
文摘Skin tissue engineering with considerable skin regeneration capability is an urgent need for the wound site.The current challenge for researchers is to develop a bionic scaffold that imitates the extracellular matrix for the regeneration of the damaged regions.In our study,poly(L-lactide-co-caprolactone)(PLCL)was blended with polyurethane(PU)to obtain nanofibrous scaffolds via electrospinning.The electrospun fibers with 50%PLCL content had a certain number of intersections and jointing points,and exhibited significantly enhanced mechani-cal properties combined with suitable porosity.Moreover,cell activities demonstrated that PU/PLCL membranes had significantly biological advantages in enhanced growth of human skin fibroblasts with spreading morphology compared with PU membranes,indicating good cytocompatibility of composite scaffolds.These findings proved that PU/PLCL electrospun membranes have great potential in applications of skin tissue engineering.
文摘The publisher regrets that ethical approval was not included in the published version.Upon request,the authors have provided the appropriate ethical approval statement,which states that the human skin fibroblasts used in this study were derived from normal skin tissues of patients after scar resection at Shanxi Bethune Hospital,and were obtained under ethical requirements.The experimental process strictly adhered to relevant ethical requirements and guidelines,and was granted approval by the ethics committee with protocol number SBQLL-2021-065 from Shanxi Bethune Hospital.
