Immune checkpoint inhibitor(ICI)–associated myocarditis(ICIAM)is a rare yet potentially life-threatening complication.Its clinical and ECG manifestations are nonspecific,posing challenges for early diagnosis and incr...Immune checkpoint inhibitor(ICI)–associated myocarditis(ICIAM)is a rare yet potentially life-threatening complication.Its clinical and ECG manifestations are nonspecific,posing challenges for early diagnosis and increasing the risk of misdiagnosis.A 61-year-old male with lung adenocarcinoma and a history of tuberculosis developed chest tightness,palpitations,elevated cardiac troponin I(cTnI),and ST-segment elevation in leads V1–V4 after three cycles of chemotherapy combined with ICI.Initially diagnosed as an acute ST-elevation myocardial infarction(STEMI),coronary angiography ruled out obstructive coronary disease.Despite standard STEMI treatment,symptoms persisted,accompanied by frequent ventricular premature beats,ventricular tachycardia,and persistently high cTnI levels,which were inconsistent with typical STEMI progression.Suspecting ICIAM,the patient was treated with low-dose methylprednisolone(40 mg/day)instead of high-dose pulse therapy(500–1000 mg/day)to reduce the risks of infection and tuberculosis reactivation.Symptoms improved,arrhythmias subsided,and cTnI levels declined rapidly.ICIAM may mimic STEMI on ECG,necessitating careful differentiation.For high-risk patients unsuitable for high-dose glucocorticoids,low-dose regimens may serve as an effective individualized treatment.展开更多
Oocyte maturation is critical for insect reproduction.Vitellogenesis,the timely production and uptake of vitellogenin(Vg),is crucial for female fecundity.Vg is synthesized in fat body and absorbed by the oocytes throu...Oocyte maturation is critical for insect reproduction.Vitellogenesis,the timely production and uptake of vitellogenin(Vg),is crucial for female fecundity.Vg is synthesized in fat body and absorbed by the oocytes through endocytosis during insect oogenesis.In the silkworm,Bombyx mori,we discovered that a nucleus-enriched long-noncoding RNA(lncRNA)lncR26319 regulates Endophilin A(EndoA)-a member of the endophilin family of endocytic proteins-through competitive binding to miR-2834.The lncR26319-miR-2834-EndoA axis was required for Vg endocytosis in the silkworm;loss of EndoA or overexpression ofmiR-2834significantlyreduced eggnumbers invirginmoths.Inaddition,accumulation of miR-2834 resulted in pupal and adult deformation and reduced fecundity in females.The expression of Vg,30-kDa(30K)protein,and egg-specific protein(Esp)decreased after knockdown of EndoA or overexpression of miR-2834,while knockdown of miR-2834 had an opposite effect on the expression of Vg,30K protein gene,and Esp.These results suggest that the lncR26319-miR-2834-EndoA axis contributes to the endocytic activity in theVguptake and leads to the normal progression of oogenesis in the silkworm.Thus,miR-2834 and EndoA are crucial for female reproduction and could be potential targets for new pest management strategies in lepidopterans.展开更多
基金supported by the Noncommunicable Chronic Diseases-National Science and Technology Major Project(2023ZD0502703)the National Natural Science Foundation of China(Nos.82570520 and 82070453)the Shandong Provincial Natural Science Foundation(ZR2025MS1241).
文摘Immune checkpoint inhibitor(ICI)–associated myocarditis(ICIAM)is a rare yet potentially life-threatening complication.Its clinical and ECG manifestations are nonspecific,posing challenges for early diagnosis and increasing the risk of misdiagnosis.A 61-year-old male with lung adenocarcinoma and a history of tuberculosis developed chest tightness,palpitations,elevated cardiac troponin I(cTnI),and ST-segment elevation in leads V1–V4 after three cycles of chemotherapy combined with ICI.Initially diagnosed as an acute ST-elevation myocardial infarction(STEMI),coronary angiography ruled out obstructive coronary disease.Despite standard STEMI treatment,symptoms persisted,accompanied by frequent ventricular premature beats,ventricular tachycardia,and persistently high cTnI levels,which were inconsistent with typical STEMI progression.Suspecting ICIAM,the patient was treated with low-dose methylprednisolone(40 mg/day)instead of high-dose pulse therapy(500–1000 mg/day)to reduce the risks of infection and tuberculosis reactivation.Symptoms improved,arrhythmias subsided,and cTnI levels declined rapidly.ICIAM may mimic STEMI on ECG,necessitating careful differentiation.For high-risk patients unsuitable for high-dose glucocorticoids,low-dose regimens may serve as an effective individualized treatment.
基金supported byNational Natural Science Foundation of China(No.31970480)Natural Science Foundation of Henan province(No.212300410063)Young Elite Scientist Sponsorship Program by China Association for Science and Technol-0gy(YESS 20150026).
文摘Oocyte maturation is critical for insect reproduction.Vitellogenesis,the timely production and uptake of vitellogenin(Vg),is crucial for female fecundity.Vg is synthesized in fat body and absorbed by the oocytes through endocytosis during insect oogenesis.In the silkworm,Bombyx mori,we discovered that a nucleus-enriched long-noncoding RNA(lncRNA)lncR26319 regulates Endophilin A(EndoA)-a member of the endophilin family of endocytic proteins-through competitive binding to miR-2834.The lncR26319-miR-2834-EndoA axis was required for Vg endocytosis in the silkworm;loss of EndoA or overexpression ofmiR-2834significantlyreduced eggnumbers invirginmoths.Inaddition,accumulation of miR-2834 resulted in pupal and adult deformation and reduced fecundity in females.The expression of Vg,30-kDa(30K)protein,and egg-specific protein(Esp)decreased after knockdown of EndoA or overexpression of miR-2834,while knockdown of miR-2834 had an opposite effect on the expression of Vg,30K protein gene,and Esp.These results suggest that the lncR26319-miR-2834-EndoA axis contributes to the endocytic activity in theVguptake and leads to the normal progression of oogenesis in the silkworm.Thus,miR-2834 and EndoA are crucial for female reproduction and could be potential targets for new pest management strategies in lepidopterans.
