Rational design and controllable synthesis of practical electrodes with high sta bility and activity at high current density for a hydrogen evolution reaction(HER)are critical for renewable and sustainable energy conv...Rational design and controllable synthesis of practical electrodes with high sta bility and activity at high current density for a hydrogen evolution reaction(HER)are critical for renewable and sustainable energy conversion.However,high-performance TiO_(2)-based electrocatalysts for HER are quite limited,and the cat alytic active centers still remain elusive.Herein,a simple strategy is demonstrated for the synthesis of TiO_(2)-carbon composite(TiO_(2)/C)with high HER performance and stability.The remarkable HER performance of TiO_(2)/C can be ascribed to the doping of carbon atoms,which leads to stronger hybridization of Ti 3d and O 2p orbitals,thus substantially improving the electrocatalytic efficiency.This study elucidates that the hydrogen evolution activity of oxide electrocatalysts can be largely improved by regulating their electronic structures by doping carbon atoms and also provides an effective strategy for designing heterostructured electro catalysts with high catalytic activity and stability at high current density for HER.展开更多
Person re-identification(Re-ID)is a fundamental subject in the field of the computer vision technologies.The traditional methods of person Re-ID have difficulty in solving the problems of person illumination,occlusion...Person re-identification(Re-ID)is a fundamental subject in the field of the computer vision technologies.The traditional methods of person Re-ID have difficulty in solving the problems of person illumination,occlusion and attitude change under complex background.Meanwhile,the introduction of deep learning opens a new way of person Re-ID research and becomes a hot spot in this field.This study reviews the traditional methods of person Re-ID,then the authors focus on the related papers about different person Re-ID frameworks on the basis of deep learning,and discusses their advantages and disadvantages.Finally,they propose the direction of further research,especially the prospect of person Re-ID methods based on deep learning.展开更多
Cantharidin(CTD)is a bioactive ingredient isolated from Cantharis vesicatoria(blister beetles),which has potential therapeutic value as an anticancer agent.Magnesium Demethylcantharidate(MDC)is a recently developed de...Cantharidin(CTD)is a bioactive ingredient isolated from Cantharis vesicatoria(blister beetles),which has potential therapeutic value as an anticancer agent.Magnesium Demethylcantharidate(MDC)is a recently developed derivative of Cantharidin(CTD),and previous studies have illustrated its excellent anticancer activity on HCC cells.However,the effect and mechanism of MDC remains unclear and need to be further studied.In particular,whether MDC can cause ER stress in HCC is still unknown.In this study,we demonstrated that endoplasmic reticulum stress(ERS)-related proteins were changed in SMMC-7721 and Bel-7402 cells after being exposed to MDC.Moreover,we found that MDC could significantly inhibit the growth of xenograft tumor in nude mice.In summary,we confirmed that MDC could induce ERS in HCC cells and thus induce apoptosis.展开更多
A simple analytical assay based on ultra-high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS)and micro-solid phase extraction(μ-SPE)techniques was established for the quantification of polypro...A simple analytical assay based on ultra-high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS)and micro-solid phase extraction(μ-SPE)techniques was established for the quantification of polypropylene glycol 4K(PPG4K)polymers in cellular samples.Afterμ-SPE,the analytes were concentrated and separated on a reversed phase C18column.Collision induced dissociation(CID)was tested and the parent ions of PPG4K polymers were fragmented with a relatively high value of declustering potential(DP)in the ion source.Numerous fragment ions associated with the structure of PPG4K were generated in ion source at m/z 58n+1([M+H]+),58n+18([M+NH_(4)]^(+)),58n+23([M+Na]+)and 58n+39([M+K]+),respectively.[M+NH_(4)]^(+)adducts were selected and further fragmented in the second quadrupole of the UHPLC-MS/MS system.Multiple reaction monitoring(MRM)transition at m/z 598.4[M+NH_(4)]^(+)(58×10+18)→175.0 was selected to determine PPG4K polymers in cellular samples owing to its excellent selectivity and higher sensitivity compared with the other MRM transitions.The developed analytical assay was successfully applied to the bioanalysis of PPG4K polymers in cellular samples.The lower limit of quantification(LLOQ)was 10 ng/mL and the limit of detection(LOD)was 2.5 ng/mL for PPG4K polymers.This study provides a novel analytical strategy for the determination of PPG4K polymers,which is coupled withμ-SPE and the selection of[M+NH_(4)]^(+)adduct strategy to enhance sensitivity.展开更多
The underlying cause of low response rates to existing immunotherapies is that tumor cells dominate tumor immune escape through surface antigen deficiency and inducing tumor immunosuppressive microenvironment(TIME).He...The underlying cause of low response rates to existing immunotherapies is that tumor cells dominate tumor immune escape through surface antigen deficiency and inducing tumor immunosuppressive microenvironment(TIME).Here,we proposed an in situ tumor cell engineering strategy to disrupt tumor immune escape at the root by restoring tumor cell MHC-I/tumor-specific antigen complex(MHC-I/TSA)expression to promote T-cell recognition and by silencing tumor cell CD55 to increase the ICOSL^(+)B-cell proportion and reverse the TIME.A doxorubicin(DOX)and dual-gene plasmid(MAC pDNA,encoding both MHC-I/ASMTNMELM and CD55-shRNA)coloaded drug delivery system(LCPN@ACD)with tumor targeting and charge/size dualeconversion properties was prepared.LCPN@ACD-induced ICD promoted DC maturation and enhanced T-cell activation and infiltration.LCPN@ACD enabled effective expression of MHC-I/TSA on tumor cells,increasing the ability of tumor cell recognition and killing.LCPN@ACD downregulated tumor cell CD55 expression,increased the proportion of ICOSL^(+)B cells and CTLs,and reversed the TIME,thus greatly improving the efficacy of aPD-1 and CAR-T therapies.The application of this in situ tumor cell engineering strategy eliminated the source of tumor immune escape,providing new ideas for solving the challenges of clinical immunotherapy.展开更多
Parkinson's disease(PD)is a neurodegenerative disease characterized by various motor and non-motor symptoms.The com-plexity of its symptoms suggests that PD is a heterogeneous neurological disorder Its pathologica...Parkinson's disease(PD)is a neurodegenerative disease characterized by various motor and non-motor symptoms.The com-plexity of its symptoms suggests that PD is a heterogeneous neurological disorder Its pathological changes are not limited to the substantia nigra-striatal system,but gradually extending to other regions including the cerebellum.The cerebellum is connected to a wide range of central nervous system regions that form essential neural circuits affected by PD.In addition,altered dopaminergic activity andα-synuclein pathology are found in the cerebellum,further suggesting its role in the PD progression.Furthermore,an increasing evidence obtained from imaging studies has demonstrated that cerebellar structure,functional connectivity,and neural metabolism are altered in PD when compared to healthy controls,as well as among different PD subtypes.This review provides a comprehensive summary of the cerebellar pathophysiology and results from neuroimaging studies related to both motor and non-motor symptoms of PD,highlighting the potential significance of cerebellar assessment in PD diagnosis,differential diagnosis,and disease monitoring.展开更多
Summary What is already known about this topic?Red and processed meat consumption has been positively related to an increased risk of diabetes in Western populations.However,the results remain inconclusive within Asia...Summary What is already known about this topic?Red and processed meat consumption has been positively related to an increased risk of diabetes in Western populations.However,the results remain inconclusive within Asian populations.What is added by this report?This dose-response meta-analysis of prospective cohort studies conducted in East Asian populations reveals a positive relation between the consumption of processed meat and increased risk of diabetes.Furthermore,a Ushaped association was identified between the consumption of unprocessed red meat and the risk of diabetes.What are the implications for public health practice?This research presents substantive evidence advocating for the reduction of processed and unprocessed red meat consumption as a viable strategy for mitigating the risk of diabetes in East Asian populations.展开更多
In a 2014 article on George Orwell's celebrated essay"A Hanging",John Rodden argues that the reader's not knowing who the condemned man is or what crimehe has committed effectively blurs the preconce...In a 2014 article on George Orwell's celebrated essay"A Hanging",John Rodden argues that the reader's not knowing who the condemned man is or what crimehe has committed effectively blurs the preconceptions that the reader might entertain,thus reinforcing the message of"the sheer futility and wrongness of taking a humanlife"(Rodden 73).The same basic argument can be made about Orwell's firstnovel,Burmese Days,in which the readers are not informed of the historical contextof British rule in Burma,or the events that have led up to the situation depicted inthe novel,or even how the relative merits and shortcomings of the British expatriatesand the Burmese locals should be understood.展开更多
基金supported bythe Natural Scientific Foundation of China(Grants 21878001,22078002,21776001,21875001,21978002,21808002,22008001,and U1710114).
文摘Rational design and controllable synthesis of practical electrodes with high sta bility and activity at high current density for a hydrogen evolution reaction(HER)are critical for renewable and sustainable energy conversion.However,high-performance TiO_(2)-based electrocatalysts for HER are quite limited,and the cat alytic active centers still remain elusive.Herein,a simple strategy is demonstrated for the synthesis of TiO_(2)-carbon composite(TiO_(2)/C)with high HER performance and stability.The remarkable HER performance of TiO_(2)/C can be ascribed to the doping of carbon atoms,which leads to stronger hybridization of Ti 3d and O 2p orbitals,thus substantially improving the electrocatalytic efficiency.This study elucidates that the hydrogen evolution activity of oxide electrocatalysts can be largely improved by regulating their electronic structures by doping carbon atoms and also provides an effective strategy for designing heterostructured electro catalysts with high catalytic activity and stability at high current density for HER.
基金supported by the Natural Science Foundation of China No.61703119,61573114Natural Science Fund of Heilongjiang Province of China No.QC2017070Fundamental Research Funds for the Central Universities of China No.HEUCFM180405.
文摘Person re-identification(Re-ID)is a fundamental subject in the field of the computer vision technologies.The traditional methods of person Re-ID have difficulty in solving the problems of person illumination,occlusion and attitude change under complex background.Meanwhile,the introduction of deep learning opens a new way of person Re-ID research and becomes a hot spot in this field.This study reviews the traditional methods of person Re-ID,then the authors focus on the related papers about different person Re-ID frameworks on the basis of deep learning,and discusses their advantages and disadvantages.Finally,they propose the direction of further research,especially the prospect of person Re-ID methods based on deep learning.
基金This work was supported by Natural Science Foundation of China(81660611)Guizhou Provincial Science&Technology Program(ZK[2022]615)+5 种基金the Science and Technology Project of Guizhou Provincial Health Commission(gzwkj2021-59)the Science&Technology Plan of Zunyi(ZSKHZC-HZ[2020]98)the Xin miao Foundation of Zunyi Medical University([2017]5733-055)Innovation Talent Team of Guizhou Science and Technology Department(QKHPTRC[2020]5007)Science and Technology Department of Zunyi City of Guizhou province of China([2020]7)and Innovation Talent Team of Zunyi(ZSKRC[2019]1).
文摘Cantharidin(CTD)is a bioactive ingredient isolated from Cantharis vesicatoria(blister beetles),which has potential therapeutic value as an anticancer agent.Magnesium Demethylcantharidate(MDC)is a recently developed derivative of Cantharidin(CTD),and previous studies have illustrated its excellent anticancer activity on HCC cells.However,the effect and mechanism of MDC remains unclear and need to be further studied.In particular,whether MDC can cause ER stress in HCC is still unknown.In this study,we demonstrated that endoplasmic reticulum stress(ERS)-related proteins were changed in SMMC-7721 and Bel-7402 cells after being exposed to MDC.Moreover,we found that MDC could significantly inhibit the growth of xenograft tumor in nude mice.In summary,we confirmed that MDC could induce ERS in HCC cells and thus induce apoptosis.
基金supported by the Open funding of the Cancer Hospital of Dalian University of Technology,China(2024-ZLKF-33)the Natural Science Foundation of Liaoning Province,China(2023-MSBA-018)the Fundamental Research Funds for the Central Universities,China(DUT21RC(3)057 and DUT23YG228)。
文摘A simple analytical assay based on ultra-high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS)and micro-solid phase extraction(μ-SPE)techniques was established for the quantification of polypropylene glycol 4K(PPG4K)polymers in cellular samples.Afterμ-SPE,the analytes were concentrated and separated on a reversed phase C18column.Collision induced dissociation(CID)was tested and the parent ions of PPG4K polymers were fragmented with a relatively high value of declustering potential(DP)in the ion source.Numerous fragment ions associated with the structure of PPG4K were generated in ion source at m/z 58n+1([M+H]+),58n+18([M+NH_(4)]^(+)),58n+23([M+Na]+)and 58n+39([M+K]+),respectively.[M+NH_(4)]^(+)adducts were selected and further fragmented in the second quadrupole of the UHPLC-MS/MS system.Multiple reaction monitoring(MRM)transition at m/z 598.4[M+NH_(4)]^(+)(58×10+18)→175.0 was selected to determine PPG4K polymers in cellular samples owing to its excellent selectivity and higher sensitivity compared with the other MRM transitions.The developed analytical assay was successfully applied to the bioanalysis of PPG4K polymers in cellular samples.The lower limit of quantification(LLOQ)was 10 ng/mL and the limit of detection(LOD)was 2.5 ng/mL for PPG4K polymers.This study provides a novel analytical strategy for the determination of PPG4K polymers,which is coupled withμ-SPE and the selection of[M+NH_(4)]^(+)adduct strategy to enhance sensitivity.
基金supported by the National Natural Science Foundation of China(82173757,81974498,82173756)the Natural Science Foundation of Shandong Province(ZR2023QH_(2)85,China)+1 种基金the Innovation Team of Shandong Higher School Youth Innovation Technology Program(2022KJ197,China)Talent Introduction(Promotion)Program of Shandong First Medical University&Shandong Academy of Medical Sciences.
文摘The underlying cause of low response rates to existing immunotherapies is that tumor cells dominate tumor immune escape through surface antigen deficiency and inducing tumor immunosuppressive microenvironment(TIME).Here,we proposed an in situ tumor cell engineering strategy to disrupt tumor immune escape at the root by restoring tumor cell MHC-I/tumor-specific antigen complex(MHC-I/TSA)expression to promote T-cell recognition and by silencing tumor cell CD55 to increase the ICOSL^(+)B-cell proportion and reverse the TIME.A doxorubicin(DOX)and dual-gene plasmid(MAC pDNA,encoding both MHC-I/ASMTNMELM and CD55-shRNA)coloaded drug delivery system(LCPN@ACD)with tumor targeting and charge/size dualeconversion properties was prepared.LCPN@ACD-induced ICD promoted DC maturation and enhanced T-cell activation and infiltration.LCPN@ACD enabled effective expression of MHC-I/TSA on tumor cells,increasing the ability of tumor cell recognition and killing.LCPN@ACD downregulated tumor cell CD55 expression,increased the proportion of ICOSL^(+)B cells and CTLs,and reversed the TIME,thus greatly improving the efficacy of aPD-1 and CAR-T therapies.The application of this in situ tumor cell engineering strategy eliminated the source of tumor immune escape,providing new ideas for solving the challenges of clinical immunotherapy.
基金supported by grants from the National Natural Science Foundation of China to WL(Nos.32220103006,and 82271524)the Liaoning Provincial Natural Science Foundation Joint Fund toTL(No.2023-MSLH-029)Shanghai Pudong New Area Health Commission Clinical Characteristic Discipline Construction Plan to TL(No.PWYts2021-02).
文摘Parkinson's disease(PD)is a neurodegenerative disease characterized by various motor and non-motor symptoms.The com-plexity of its symptoms suggests that PD is a heterogeneous neurological disorder Its pathological changes are not limited to the substantia nigra-striatal system,but gradually extending to other regions including the cerebellum.The cerebellum is connected to a wide range of central nervous system regions that form essential neural circuits affected by PD.In addition,altered dopaminergic activity andα-synuclein pathology are found in the cerebellum,further suggesting its role in the PD progression.Furthermore,an increasing evidence obtained from imaging studies has demonstrated that cerebellar structure,functional connectivity,and neural metabolism are altered in PD when compared to healthy controls,as well as among different PD subtypes.This review provides a comprehensive summary of the cerebellar pathophysiology and results from neuroimaging studies related to both motor and non-motor symptoms of PD,highlighting the potential significance of cerebellar assessment in PD diagnosis,differential diagnosis,and disease monitoring.
基金Supported by grants 81930124,82192902,and 82021005 from the National Natural Science Foundation of Chinagrant 2022YFC3600600 from the National Key Research and Development Program of China.
文摘Summary What is already known about this topic?Red and processed meat consumption has been positively related to an increased risk of diabetes in Western populations.However,the results remain inconclusive within Asian populations.What is added by this report?This dose-response meta-analysis of prospective cohort studies conducted in East Asian populations reveals a positive relation between the consumption of processed meat and increased risk of diabetes.Furthermore,a Ushaped association was identified between the consumption of unprocessed red meat and the risk of diabetes.What are the implications for public health practice?This research presents substantive evidence advocating for the reduction of processed and unprocessed red meat consumption as a viable strategy for mitigating the risk of diabetes in East Asian populations.
文摘In a 2014 article on George Orwell's celebrated essay"A Hanging",John Rodden argues that the reader's not knowing who the condemned man is or what crimehe has committed effectively blurs the preconceptions that the reader might entertain,thus reinforcing the message of"the sheer futility and wrongness of taking a humanlife"(Rodden 73).The same basic argument can be made about Orwell's firstnovel,Burmese Days,in which the readers are not informed of the historical contextof British rule in Burma,or the events that have led up to the situation depicted inthe novel,or even how the relative merits and shortcomings of the British expatriatesand the Burmese locals should be understood.
