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新型虎源猫杯状病毒荧光定量PCR检测方法的建立及应用
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作者 梅隐 高向向 +7 位作者 刘诗云 吕梦娜 刘敏婷 侯丽萍 李婉萍 单芬 陈武 周妞 《野生动物学报》 北大核心 2025年第4期848-855,共8页
在本团队前期研究中,通过宏病毒组学测序与生物信息学分析,从一例死亡华南虎(Panthera tigris amoyensis)组织病料中鉴定出一株新型虎源猫杯状病毒(Feline calicivirus isolated from tiger,TCaV),命名为LYH1224株。为实现对该病毒的快... 在本团队前期研究中,通过宏病毒组学测序与生物信息学分析,从一例死亡华南虎(Panthera tigris amoyensis)组织病料中鉴定出一株新型虎源猫杯状病毒(Feline calicivirus isolated from tiger,TCaV),命名为LYH1224株。为实现对该病毒的快速检测,基于TCaV LYH1224株基因组序列设计特异性引物,经反应体系与条件优化,构建了TCaV绝对荧光定量PCR检测方法,并开展了敏感性、特异性和重复性评估。同时,利用该方法对本实验室保存的大型猫科(Felidae)动物临床样品进行检测。结果显示:所建立的方法可特异性扩增新型TCaV基因序列;经优化,引物对4160S/4270A在最佳反应体系与程序下,C_(t)值与标准品拷贝数浓度的对数呈现良好的线性关系,扩增效率(E)达101.8%,相关系数(R^(2))为0.996,最低检测限为2×10^(1)拷贝/反应,且组内与组间重复试验变异系数(C_(V))均小于2.50%;在大型猫科动物临床样品检测中,阳性样品检出率为7.59%(6/79),个体阳性率为2.82%(2/71),且2只阳性个体来自同一保育机构,证实新型TCaV已在华南虎种群中小范围传播。本研究建立的检测方法特异性强、灵敏度高,重复性和实用性佳,为我国大型猫科动物新型TCaV的快速检测和日常监测提供了有效工具。 展开更多
关键词 新型虎源猫杯状病毒 华南虎 荧光定量PCR 大型猫科动物 临床应用
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岭南浮蛙在珠三角地区的分布补充和保育建议
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作者 石燕香 李远航 +4 位作者 王冰洋 赵一阳 梅隐 卢雨宏 林石狮 《生物学通报》 2025年第1期1-4,共4页
通过形态学和分子序列比对,确认采自广东省东莞市水濂山和大岭山的2个两栖动物标本应为近年才被科学描述的新种岭南浮蛙(Occidozyga lingnanica Lyu & Wang,2022)。本研究增加了岭南浮蛙在珠江口以东地区的分布点,提供了新地理种群... 通过形态学和分子序列比对,确认采自广东省东莞市水濂山和大岭山的2个两栖动物标本应为近年才被科学描述的新种岭南浮蛙(Occidozyga lingnanica Lyu & Wang,2022)。本研究增加了岭南浮蛙在珠江口以东地区的分布点,提供了新地理种群的分子数据,科学描述了新采标本,讨论了珠三角地区岭南浮蛙的受胁因素并提供了保育建议。 展开更多
关键词 保育 浮蛙亚科 生境退化 岭南浮蛙
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离心式清洁风机的优化设计 被引量:4
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作者 吴恩启 梅寅 +1 位作者 张士恒 陈玥 《流体机械》 CSCD 北大核心 2023年第4期66-72,85,共8页
针对纺织车间中清洁风机气动性能不足的问题,采用数值模拟与试验结合的方法对其进行研究。以静压、风量和效率为主要参数,采取分组优化的方式分别对叶片叶形和进出口安装角进行设计,并通过数值计算对各方案风机的气动性能及其流场进行... 针对纺织车间中清洁风机气动性能不足的问题,采用数值模拟与试验结合的方法对其进行研究。以静压、风量和效率为主要参数,采取分组优化的方式分别对叶片叶形和进出口安装角进行设计,并通过数值计算对各方案风机的气动性能及其流场进行分析。结果表明:Bezier函数拟合的新叶形,叶片流道面积增加9.4%,迎风面曲率和宽度的加大,使得叶道内流场分布优于原叶片,压力分布更趋平滑,对静压提升效果最明显,相比原风机增幅为10.9%;以降低流力损失为目标,通过粒子群寻优法知,进出口角的适当改变对风量、效率的提升效果最明显,相比原风机增幅分别为7.2%,5.1%。结合两组方案的最优解,确定最终风机模型。优化后风机的静压提高12.9%,风量提高8.1%,效率提高7.2%,大于分组设计中各方案单一优化的结果,符合实际生产需求。分组优化可以避免各动静部件之间的协同干涉作用,最大限度的提升风机性能。 展开更多
关键词 清洁风机 分组优化 粒子群寻优 数值模拟
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植物微生物组生态功能与群落构建过程研究进展 被引量:22
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作者 曾青 熊超 +3 位作者 尹梅 葛安辉 韩丽丽 张丽梅 《生物多样性》 CAS CSCD 北大核心 2023年第4期182-198,共17页
植物各个器官表面及内部定殖着高度多样化的微生物群落,这些微生物与植物长期共进化,作为宿主植物的“共生功能体”(holobiont)在植物生长发育、养分吸收、病害抵御和环境胁迫适应性等方面发挥了重要作用。得益于近10年来多组学技术的... 植物各个器官表面及内部定殖着高度多样化的微生物群落,这些微生物与植物长期共进化,作为宿主植物的“共生功能体”(holobiont)在植物生长发育、养分吸收、病害抵御和环境胁迫适应性等方面发挥了重要作用。得益于近10年来多组学技术的发展和应用,有关植物微生物群落的多样性、组成和功能特征、群落构建的驱动因素和植物–微生物互作机制等方面研究取得了一系列重要进展。然而,与土壤微生物组相比,目前对植物微生物组的认识及其应用尚且不足。本文系统总结了植物微生物组的组成特征,植物微生物在调节植物生长发育、促进养分吸收、提高病害抵御能力及环境胁迫适应性等方面的功能及作用机制,从宿主选择、环境因子以及生物互作3个方面总结了驱动植物微生物群落构建的因素,并着重阐述了植物–微生物互作如何塑造植物微生物群落以及如何调节对植物的有益功能。此外,我们对未来植物微生物组研究和应用面临的挑战进行了展望,如核心微生物组挖掘和合成群落构建,植物–微生物互作的分子调控机制,植物微生物群落水平上的互作机制等。深入理解植物微生物群落特征、生态功能以及构建过程对于精准调控植物微生物组以提高植物适应性和生产力以及维持生态系统健康具有重要意义。 展开更多
关键词 表生微生物 内生微生物 宿主选择 促生 抗病 植物–微生物互作
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Preparation by Wet-spinning and Properties Polyaniline-Polyethersulfone Fibers for Flexible Supercapacitor Electrodes
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作者 mei yin LIU Hang +3 位作者 TANG Haixiong HE yining WANG Di LI Qiong 《Journal of Wuhan University of Technology(Materials Science)》 SCIE EI CAS CSCD 2024年第6期1406-1415,共10页
Flexible PANI-Polyethersulfone (PES) fibers were fabricated using the wet-spinning technique.PANI particles were uniformly distributed within the matrix and micropores formed by the phase separation of PES,which preve... Flexible PANI-Polyethersulfone (PES) fibers were fabricated using the wet-spinning technique.PANI particles were uniformly distributed within the matrix and micropores formed by the phase separation of PES,which prevented PANI particles aggregation and facilitated the formation of continuous ion transport channels.The experimental results reveals that the electrochemical performance of the fiber electrode material is optimal when the concentration of PES in the spinning solution is 15wt%.The assembled supercapacitor exhibits a commendable specific area capacitance of 162.75 mF·cm^(-2) at a current density of 0.5 mA·cm^(-2) and achieves an energy density of 14.47 mWh·cm^(-2) at a power density of 321.69 mW·cm^(-2).The capacitor retains 98.1% of its capacitance after 1 000 bending cycles.Therefore,the prepared fibers have good electrochemical properties and flexibility,and this simple and efficient preparation method is promising for the scalable production of flexible electrodes. 展开更多
关键词 POLYANILINE POLYETHERSULFONE WET-SPINNING flexible supercapacitor
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老年肺癌患者静脉血栓栓塞症风险预测工具的比较研究 被引量:8
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作者 梅茵 黄立学 +1 位作者 刘晔 陈伟 《中华护理杂志》 CSCD 北大核心 2023年第19期2333-2338,共6页
目的比较4种静脉血栓栓塞症(venous thromboembolism,VTE)风险评估工具对老年肺癌患者发生VTE的预测效能。方法连续选择2020年1月—2022年6月,北京市某三级甲等医院呼吸与危重症医学科住院的老年肺癌患者,采用Caprini评分、Padua评分、K... 目的比较4种静脉血栓栓塞症(venous thromboembolism,VTE)风险评估工具对老年肺癌患者发生VTE的预测效能。方法连续选择2020年1月—2022年6月,北京市某三级甲等医院呼吸与危重症医学科住院的老年肺癌患者,采用Caprini评分、Padua评分、Khorana评分和COMPASS-CAT评分4种风险评估工具进行血栓风险评估,并随访至入组后第6个月。根据各评分对应截断值将患者分为低中危组和高危组,比较两组之间发生VTE的差异。利用受试者工作特征曲线下面积评价4种VTE预测工具对老年肺癌患者远期发生VTE的预测效能。结果纳入111例老年肺癌患者,入组后6个月内,20例(18.0%)发生VTE。VTE组和未发生VTE组D-二聚体的比较,差异有统计学意义(P<0.05)。Caprini评分高危组VTE发生率显著高于中危组,差异有统计学意义(P=0.022),Padua评分高危组VTE发生率显著高于低危组,差异有统计学意义(P<0.001),而Khorana和COMPASS-CAT评分高危组VTE发生率均高于非高危组,但差异无统计学意义(均P>0.05)。Padua评分、Caprini评分、Khorana评分和COMPASS-CAT评分对应的受试者工作特征曲线下面积依次为0.805(95%CI:0.672~0.937)、0.757(95%CI:0.643~0.871)、0.552(95%CI:0.401~0.704)和0.515(95%CI:0.356~0.673)。结论老年肺癌患者VTE发生率高,亟须通过风险预测工具早期发现高危人群。Padua评分系统对老年肺癌患者VTE风险的预测效能优于Caprini评分、Khorana评分和COMPASS-CAT评分。 展开更多
关键词 老年人 肺癌 静脉血栓栓塞症 风险评估 护理
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Finite element modeling of thermo-active diaphragm walls 被引量:3
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作者 Yi RUI mei yin 《Frontiers of Structural and Civil Engineering》 SCIE EI CSCD 2020年第3期646-663,共18页
There are two major challenges faced by modern society:energy security,and lowering carbon dioxide gas emissions.Thermo-active diaphragm walls have a large potential to remedy one of these problems,since they are a re... There are two major challenges faced by modern society:energy security,and lowering carbon dioxide gas emissions.Thermo-active diaphragm walls have a large potential to remedy one of these problems,since they are a renewable energy technology that uses underground infrastructure as a heat exchange medium.However,extensive research is required to determine the effects of cyclic heating and cooling on their geotechnical and structural performance.In this paper,a series of detailed finite element analyses are carried out to capture the fully coupled thermo-hydro-mechanical response bf the ground and diaphragm wall.It is demonstrated that the thermal operation of the diaphragm wall causes changes in soil temperature,thermal expansion/shrinkage of pore water,and total stress applied on the diaphragm wall.These,in turmn,cause displacements of the diaphragm wall and variations of the bending moments.However,these effects on the performance of diaphragm wall are not significant.The thermally induced bending strain is mainly governed by the temperature differential and uneven thermal expansion/shrinkage across the wall. 展开更多
关键词 thermo-active diaphragm wall finite element analysis thermo-hyro-mechanical coupling ground source heat pump
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Effect of stress path on the mechanical properties of calcareous sand 被引量:2
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作者 Houzhen Wei Hao Liu +4 位作者 Xiaoxiao Li Zhao Tao Yongjie Wu Jianhua Shen mei yin 《Underground Space》 SCIE EI CSCD 2023年第2期20-30,共11页
Calcareous sand is widely observed in the foundation of off-shore infrastructure.Although a lot of research has been carried out on the mechanical properties of calcareous sand,study into the influence of the stress–... Calcareous sand is widely observed in the foundation of off-shore infrastructure.Although a lot of research has been carried out on the mechanical properties of calcareous sand,study into the influence of the stress–strain path on the mechanical behaviour of calcareous sand is very limited.In this study,a series of triaxial tests were performed on calcareous sand under three different stress paths.The particle morphology of calcareous sand before and after the tests,the stress–strain relationship under different stress paths,and the char-acteristics of shear strength and deformation were investigated.The results show that the consolidation pressure and stress path have significant effects on the volume strain,strength,and particle breakage of calcareous sand.In addition,the underlying mechanisms of the different behaviours of calcareous sand observed in this study were discussed. 展开更多
关键词 Calcareous sand Triaxial test Stress path Particle breakage
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Distributed fiber optic monitoring of a CFA pile with a central reinforcement bar bundle 被引量:1
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作者 Yi RUI Nicholas de BATTISTA +2 位作者 Cedric KECHAVARZI Xiaomin XU mei yin 《Frontiers of Structural and Civil Engineering》 SCIE EI CSCD 2021年第1期167-176,共10页
In this paper,we present an application of distributed fiber optic sensor(DFOS)technology to measure the strain of a continuous flight auger(CFA)test pile with a central reinforcement bar bundle,during a static load t... In this paper,we present an application of distributed fiber optic sensor(DFOS)technology to measure the strain of a continuous flight auger(CFA)test pile with a central reinforcement bar bundle,during a static load test carried out in London.Being distributed in nature,DFOS gives much more information about the pile performance as compared to traditional point sensors,such as identifying cross-sectional irregularities or other anomalies.The strain profiles recorded along the depth of the piles from the DFOS were used to calculate pile deformation(contraction),shaft friction,and tip resistance under various loads.Based on this pile load test,a finite element(FE)analysis was performed using a one-dimensional nonlinear load-transfer model.Calibrated by the shaft friction and tip resistance derived from the monitored data,the FE model was able to simulate the pile and soil performance during the load testing with good accuracy.The effect of the reinforcement cage and central reinforcement bar bundle were investigated,and it was found that the addition of a reinforcement cage would reduce the pile settlement by up to 20%. 展开更多
关键词 continuous flight auger pile static load test central reinforcement bar bundle distributed fiber optic sensor finite element load transfer
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Optimization of pipe circuits in energy tunnels 被引量:1
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作者 Jie He mei yin +1 位作者 Xiangyang Wei Zhenhuang Wu 《Underground Space》 SCIE EI CSCD 2023年第6期166-182,共17页
Geothermal energy is a kind of green and renewable energy.Conventionally,ground source heat pumps can be used to harvest geothermal energy from the subsurface.To reduce the initial investment,a good solution is to use... Geothermal energy is a kind of green and renewable energy.Conventionally,ground source heat pumps can be used to harvest geothermal energy from the subsurface.To reduce the initial investment,a good solution is to use tunnel linings as heat exchangers to extract/dump heat.This special infrastructure is called an energy tunnel.In addition to the thermal performance,the impact of pipe network configuration on thermal efficiency is still challenging in the design of energy tunnels.To solve this problem,this study makes the first attempt to carry out research on the optimization of pipe circuits in energy tunnels by a series of numerical analyses.A fully coupled thermo-hydraulic 3D finite element model is established to investigate the response of tunnel-soil interaction under cyclical thermal loading(initial soil temperature varies from 8C to 18C),as well as the thermal transient interactions among air,absorber pipe,tunnel linings and ground,to quantify the amount of useful heat that can be extracted from the tunnel and the ground.On the other hand,the influence of 3 various heat-carrying pipes layout is also investigated.It is found that higher heat transfer efficiency can be obtained when the entrance and exit of pipelines are located below the tunnel in the study.The spatial location of pipelines will also affect the exchanged heat output. 展开更多
关键词 Energy tunnel Thermal efficiency Finite element model Heat transfer
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Study on the long-term performance of shield tunnel passing through the gas-bearing strata
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作者 Jie He Xiangyang Wei mei yin 《Underground Space》 SCIE EI CSCD 2023年第2期1-19,共19页
When the shield tunnel passes through the gas-bearing strata,gas and water leakage may occur depending on the sealing performance of the segment joints.This process involves the complex multiphase seepage flow phenome... When the shield tunnel passes through the gas-bearing strata,gas and water leakage may occur depending on the sealing performance of the segment joints.This process involves the complex multiphase seepage flow phenomenon in unsaturated soil.In this study,a fully coupled solid-liquid-gas model of the GIL Utility Tunnel was established to investigate the influence of the high-pressure gas on the mechanical properties of the tunnel segments and joints.The constitutive model of the Extended Barcelona Basic Model was imple-mented to simulate the effect of the seepage process on soil deformation.The results show that significant upward displacement occurred in the gas reservoir and its overlying strata,and the maximum displacement reached 30 mm.In addition,during the leakage of the gas and the water,an increase in the average soil effective stress was observed.It would induce a reduction in the suction and expansion of the yield surface.The tunnel tended to be stable from 20 years onwards,thus the soil deformation due to the water leakage only occurred at the early stage.In addition,the joint opening under the most unfavorable internal force combination was 0.69 mm,and the correspond-ing bolt stress was 119.5 MPa,which is below the yield limit.The results of this study help to understand the influence of high-pressure gas on tunnel safety and the sealing performance of the joints. 展开更多
关键词 A coupled solid-liquid-gas model Unsaturated soil Multiphase seepage Gas-bearing strata Shield tunnel FEM
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Numerical and experimental analyses of methane leakage in shield tunnel
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作者 Jie HE Hehua ZHU +3 位作者 Xiangyang WEI Rui JIN Yaji JIAO mei yin 《Frontiers of Structural and Civil Engineering》 SCIE EI CSCD 2023年第7期1011-1020,共10页
Tunnels constructed in gas-bearing strata are affected by the potential leakage of harmful gases,such as methane gas.Based on the basic principles of computational fluid dynamics,a numerical analysis was performed to ... Tunnels constructed in gas-bearing strata are affected by the potential leakage of harmful gases,such as methane gas.Based on the basic principles of computational fluid dynamics,a numerical analysis was performed to simulate the ventilation and diffusion of harmful gases in a shield tunnel,and the effect of ventilation airflow speed on the diffusion of harmful gases was evaluated.As the airflow speed increased from 1.8 to 5.4 m/s,the methane emission was diluted,and the methane accumulation was only observed in the area near the methane leakage channels.The influence of increased ventilation airflow velocity was dominant for the ventilation modes with two and four fans.In addition,laboratory tests on methane leakage through segment joints were performed.The results show that the leakage process can be divided into“rapid leakage”and“slight leakage”,depending on the leakage pressure and the state of joint deformation.Based on the numerical and experimental analysis results,a relationship between the safety level and the joint deformation is established,which can be used as guidelines for maintaining utility tunnels. 展开更多
关键词 shield tunnel harmful gas leakage numerical analysis laboratory test
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