目的系统评价衰弱老年人糖尿病的患病情况。方法计算机检索PubMed、EMbase、Web of Science、CBM、WanFang Data、VIP和CNKI数据库,搜集有关衰弱老年人糖尿病患病率的横断面研究,检索时限均为建库至2020年11月。由2名研究者独立筛选文...目的系统评价衰弱老年人糖尿病的患病情况。方法计算机检索PubMed、EMbase、Web of Science、CBM、WanFang Data、VIP和CNKI数据库,搜集有关衰弱老年人糖尿病患病率的横断面研究,检索时限均为建库至2020年11月。由2名研究者独立筛选文献、提取资料并评价纳入研究的偏倚风险后,采用Stata 15.0软件进行Meta分析。结果最终纳入21个横断面研究,包括22403例研究对象。Meta分析结果显示,衰弱老年人糖尿病的总患病率为34%[95%CI(26%,43%)]。亚组分析结果显示,亚洲(37%)和南美洲(33%)地区衰弱老年人糖尿病患病率高于欧洲(27%)及北美洲地区(18%);采用躯体性衰弱筛查工具(35%)诊断为衰弱老年人的糖尿病患病率高于使用综合性衰弱筛查工具(28%)诊断衰弱的老年人;门诊(67%)和医院(62%)衰弱老年人糖尿病患病率高于社区(31%)。结论现有证据表明,衰弱老年人糖尿病患病率较高。展开更多
目的系统评价衰弱和直立性低血压发生风险的关系。方法计算机检索PubMed、EMbase、Web of Science、CBM、CNKI、VIP和WanFang Data数据库,搜集国内外关于衰弱和直立性低血压发生关系的相关性研究,检索时限均从建库至2020年7月7日。由2...目的系统评价衰弱和直立性低血压发生风险的关系。方法计算机检索PubMed、EMbase、Web of Science、CBM、CNKI、VIP和WanFang Data数据库,搜集国内外关于衰弱和直立性低血压发生关系的相关性研究,检索时限均从建库至2020年7月7日。由2位评价员独立筛选文献、提取资料并评价纳入研究的偏倚风险后,采用RevMan 5.3软件进行Meta分析。结果共纳入5个研究,包括8671例患者。Meta分析结果显示:与非衰弱组相比,衰弱前期[OR=1.04,95%CI=(0.99,1.09),P=0.11]和衰弱[OR=1.02,95%CI=(0.92,1.13),P=0.70]不会增加直立性低血压的患病风险。亚组分析结果显示:采用不同的衰弱评估工具(Fried量表、临床衰弱量表和衰弱指数),不同样本量大小(<500例或者≥500例),不同地区(欧洲、亚洲和美洲),研究对象的不同来源(医院和社区),衰弱均不会增加直立性低血压患病风险。结论当前证据显示,衰弱不会增加直立性低血压的患病风险。受纳入研究数量和质量的限制,上述结论尚需更多高质量研究予以验证。展开更多
Objective:To investigate hemodynamic change of patients with cirrhosis by using Color Doppler ultrasound technique and to explore the significance of change in the content of vasoactive substances-plasma endothelin-1(...Objective:To investigate hemodynamic change of patients with cirrhosis by using Color Doppler ultrasound technique and to explore the significance of change in the content of vasoactive substances-plasma endothelin-1(ET-1)and calcitonin gene-related peptide(CGRP).Methods:A total of 178 cases with cirrhosis were regarded as study groups,and were divided into three degrees:A,B and C according to child-pugh and meanwhile 60 cases were regarded as normal control group.Portal vein and splenic vein of patients were explored by adopting Color Doppler ultrasound technique,related indexes were recorded and the blood flow as well as their ration in the two groups was calculated.Radio immunoassay was adopted to detect the content of plasma ET-1 and CGRP in both study group and contrast group.Results:Compared with the healthy cases in the contrast group,there was abnormal hemodynamics in the system of portal vein of patients with cirrhosis and the content of plasma ET-1 and CGRP was increased obviously.In the Child-Pugh liver function grades,the content of ET-1 and CGRP was increased as the degree of cirrhosis became more and more serious.There was no significant difference in the comparison between those without ascites and those in contrast group(P>0.05),the content of plasma ET-1and CGRP in patients without ascites was increased remarkably.Besides,there was positive correlation between the content of plasma ET-1 and CGRP and Dpv,Dsv and Qsv.Conclusion:Dotection of abnormal hemodynamics of portal vein and splenic vein by Color Doppler ultrasound technique can be one of the means for diagnosis of hypertension.Plasma ET-1 and CGRP of patients with cirrhosis reflect the serious degree of the damage in live function and play an important role in the formation and development of portal hypertension.展开更多
Objective:To investigate the role of epidermal growth factor(EGF) in the pathogenesis of uterine leiomyomas.Methods:Human myometrial smooth muscle cells(HM-SMCs) and smooth muscle cells of human uterine leiomyomas(HL-...Objective:To investigate the role of epidermal growth factor(EGF) in the pathogenesis of uterine leiomyomas.Methods:Human myometrial smooth muscle cells(HM-SMCs) and smooth muscle cells of human uterine leiomyomas(HL-SMCs) were separated from patients' specimens and cultured.After processed by EGF or PD98059(inhibitor of MKK/MEK) +EGF,the proliferation rate of both SMCs was detected by BrdU method and the phosphorylation level of p44/42 mitogen-activated protein kinase(MAPK) was determined by Western-blot.After different processing time by EGF,the phosphorylation levels of p44/42 MAPK and AKT and p27 expression level in both SMCs were detected by Western-blot.Results:EGF could significantly promote HL-SMCs proliferation and PD98059 could inhibit this effect(P<0.05);besides,PD98059 could inhibit the increase of the phosphorylation level of p44/42 MAPK in both SMCs induced by EGF.When the processing time by EGF was over 15 min,the phosphorylation levels of p44/42 MAPK and AKT in both SMCs decreased sharply and were close to zero:p27 expression in HM-SMCs raised significantly while the upregulation in HL-SMCs was little.Conclusions:EGF could not cause activation of EGFR because of the dephosphorylation of p44/42 MAPK and AKT in HL-SMCs,which caused p27 expression insufficiently and cell cycle dysregulation.展开更多
Objective:To discuss the liver function damage mechanism of patients with clonorchiasis by analyzing the ultrasound characteristics,liver function,change of the serum inflammatory factors and cell apoptosis factors.Me...Objective:To discuss the liver function damage mechanism of patients with clonorchiasis by analyzing the ultrasound characteristics,liver function,change of the serum inflammatory factors and cell apoptosis factors.Methods:Color Doppler ultrasound technique was adopted to detect the portal vein and blood flow change of patients with clonorchiasis;ELISA was used to determine the level of different serum inflammatory factors.The levels of serum total bilirubin,serum albumin and glutamic-pyruvic transaminase were detected by automatic biochemical analyzer.Western blot was used to determine the expression of proteins relevant to apoptosis.Results:Compared with the health control group,the trunk diameter of portal vein and the thickness of spleen,as well as the hepatic artery pulsation index of clonorchiasis patients increased obviously,the mean blood flow velocity of portal vein(P<0.05 or P<0.01) decreased.The content of total bilirubin and transaminase in plasma increased significantly,but albumin decreased(P<0.05).Levels of TNF-α,IL-6 and IFN-γ increased remarkably,and the level of every factor was significantly different among patients with Child-Pugh Ⅰ,Child-Pugh Ⅱ and Child-Pugh Ⅲ classification of liver function(P<0.05 or P<0.01).With the exacerbation of liver dysfunction,levels of TNF-α,IL-6 and IFN-γ gradually increased(P<0.05).Compared with the healthy control group,the expression quantity of apoptosis protein Fas,FasL,Bax and Caspase-3 increased significantly(P<0.05 or P<0.01),but Bcl-2 decreased(P<0.05).Conclusions:Changes of ultrasonic characteristics and liver dysfunction,caused by liver fluke infection,may be related to that both inflammatory response and apoptosis response have participated in the pathogenic process and liver damage course of clonorchiasis.展开更多
文摘目的系统评价衰弱老年人糖尿病的患病情况。方法计算机检索PubMed、EMbase、Web of Science、CBM、WanFang Data、VIP和CNKI数据库,搜集有关衰弱老年人糖尿病患病率的横断面研究,检索时限均为建库至2020年11月。由2名研究者独立筛选文献、提取资料并评价纳入研究的偏倚风险后,采用Stata 15.0软件进行Meta分析。结果最终纳入21个横断面研究,包括22403例研究对象。Meta分析结果显示,衰弱老年人糖尿病的总患病率为34%[95%CI(26%,43%)]。亚组分析结果显示,亚洲(37%)和南美洲(33%)地区衰弱老年人糖尿病患病率高于欧洲(27%)及北美洲地区(18%);采用躯体性衰弱筛查工具(35%)诊断为衰弱老年人的糖尿病患病率高于使用综合性衰弱筛查工具(28%)诊断衰弱的老年人;门诊(67%)和医院(62%)衰弱老年人糖尿病患病率高于社区(31%)。结论现有证据表明,衰弱老年人糖尿病患病率较高。
文摘目的系统评价衰弱和直立性低血压发生风险的关系。方法计算机检索PubMed、EMbase、Web of Science、CBM、CNKI、VIP和WanFang Data数据库,搜集国内外关于衰弱和直立性低血压发生关系的相关性研究,检索时限均从建库至2020年7月7日。由2位评价员独立筛选文献、提取资料并评价纳入研究的偏倚风险后,采用RevMan 5.3软件进行Meta分析。结果共纳入5个研究,包括8671例患者。Meta分析结果显示:与非衰弱组相比,衰弱前期[OR=1.04,95%CI=(0.99,1.09),P=0.11]和衰弱[OR=1.02,95%CI=(0.92,1.13),P=0.70]不会增加直立性低血压的患病风险。亚组分析结果显示:采用不同的衰弱评估工具(Fried量表、临床衰弱量表和衰弱指数),不同样本量大小(<500例或者≥500例),不同地区(欧洲、亚洲和美洲),研究对象的不同来源(医院和社区),衰弱均不会增加直立性低血压患病风险。结论当前证据显示,衰弱不会增加直立性低血压的患病风险。受纳入研究数量和质量的限制,上述结论尚需更多高质量研究予以验证。
基金supported by a grant of New Century Excellent Talents in University,Ministry of Education China(No.NCET-11-0949)
文摘Objective:To investigate hemodynamic change of patients with cirrhosis by using Color Doppler ultrasound technique and to explore the significance of change in the content of vasoactive substances-plasma endothelin-1(ET-1)and calcitonin gene-related peptide(CGRP).Methods:A total of 178 cases with cirrhosis were regarded as study groups,and were divided into three degrees:A,B and C according to child-pugh and meanwhile 60 cases were regarded as normal control group.Portal vein and splenic vein of patients were explored by adopting Color Doppler ultrasound technique,related indexes were recorded and the blood flow as well as their ration in the two groups was calculated.Radio immunoassay was adopted to detect the content of plasma ET-1 and CGRP in both study group and contrast group.Results:Compared with the healthy cases in the contrast group,there was abnormal hemodynamics in the system of portal vein of patients with cirrhosis and the content of plasma ET-1 and CGRP was increased obviously.In the Child-Pugh liver function grades,the content of ET-1 and CGRP was increased as the degree of cirrhosis became more and more serious.There was no significant difference in the comparison between those without ascites and those in contrast group(P>0.05),the content of plasma ET-1and CGRP in patients without ascites was increased remarkably.Besides,there was positive correlation between the content of plasma ET-1 and CGRP and Dpv,Dsv and Qsv.Conclusion:Dotection of abnormal hemodynamics of portal vein and splenic vein by Color Doppler ultrasound technique can be one of the means for diagnosis of hypertension.Plasma ET-1 and CGRP of patients with cirrhosis reflect the serious degree of the damage in live function and play an important role in the formation and development of portal hypertension.
基金supported by Program for New Century Excellent Talents in University(NCET-11-0949)
文摘Objective:To investigate the role of epidermal growth factor(EGF) in the pathogenesis of uterine leiomyomas.Methods:Human myometrial smooth muscle cells(HM-SMCs) and smooth muscle cells of human uterine leiomyomas(HL-SMCs) were separated from patients' specimens and cultured.After processed by EGF or PD98059(inhibitor of MKK/MEK) +EGF,the proliferation rate of both SMCs was detected by BrdU method and the phosphorylation level of p44/42 mitogen-activated protein kinase(MAPK) was determined by Western-blot.After different processing time by EGF,the phosphorylation levels of p44/42 MAPK and AKT and p27 expression level in both SMCs were detected by Western-blot.Results:EGF could significantly promote HL-SMCs proliferation and PD98059 could inhibit this effect(P<0.05);besides,PD98059 could inhibit the increase of the phosphorylation level of p44/42 MAPK in both SMCs induced by EGF.When the processing time by EGF was over 15 min,the phosphorylation levels of p44/42 MAPK and AKT in both SMCs decreased sharply and were close to zero:p27 expression in HM-SMCs raised significantly while the upregulation in HL-SMCs was little.Conclusions:EGF could not cause activation of EGFR because of the dephosphorylation of p44/42 MAPK and AKT in HL-SMCs,which caused p27 expression insufficiently and cell cycle dysregulation.
基金supported by Trans-Century Training Programme Foundation for the Talents by the Ministry of Education (NO.NCET-11-0949)
文摘Objective:To discuss the liver function damage mechanism of patients with clonorchiasis by analyzing the ultrasound characteristics,liver function,change of the serum inflammatory factors and cell apoptosis factors.Methods:Color Doppler ultrasound technique was adopted to detect the portal vein and blood flow change of patients with clonorchiasis;ELISA was used to determine the level of different serum inflammatory factors.The levels of serum total bilirubin,serum albumin and glutamic-pyruvic transaminase were detected by automatic biochemical analyzer.Western blot was used to determine the expression of proteins relevant to apoptosis.Results:Compared with the health control group,the trunk diameter of portal vein and the thickness of spleen,as well as the hepatic artery pulsation index of clonorchiasis patients increased obviously,the mean blood flow velocity of portal vein(P<0.05 or P<0.01) decreased.The content of total bilirubin and transaminase in plasma increased significantly,but albumin decreased(P<0.05).Levels of TNF-α,IL-6 and IFN-γ increased remarkably,and the level of every factor was significantly different among patients with Child-Pugh Ⅰ,Child-Pugh Ⅱ and Child-Pugh Ⅲ classification of liver function(P<0.05 or P<0.01).With the exacerbation of liver dysfunction,levels of TNF-α,IL-6 and IFN-γ gradually increased(P<0.05).Compared with the healthy control group,the expression quantity of apoptosis protein Fas,FasL,Bax and Caspase-3 increased significantly(P<0.05 or P<0.01),but Bcl-2 decreased(P<0.05).Conclusions:Changes of ultrasonic characteristics and liver dysfunction,caused by liver fluke infection,may be related to that both inflammatory response and apoptosis response have participated in the pathogenic process and liver damage course of clonorchiasis.
