Background:Drug resistance is increasing in gastrointestinal parasitosis;it is therefore essential to identify new anthelmintic molecules.Objective:The study aims to evaluate the anthelmintic activity of extracts and ...Background:Drug resistance is increasing in gastrointestinal parasitosis;it is therefore essential to identify new anthelmintic molecules.Objective:The study aims to evaluate the anthelmintic activity of extracts and fractions of leaves of Saba sene-galensis(A.DC.)Pichon(Apocynaceae)against L_(3)larvae of Caenorhabditis elegans,a free-living helminth model and to highlight potential anthelmintic molecules by dereplication strategy of molecular networking.Methods:The methanolic extract,ethanolic extract and aqueous extract of the plant’s leaves were carried out,followed by flash chromatography on silica gel for by fractionation,HPLC-MS/MS analysis.The tandem mass data were used for generation of molecular network.The anthelmintic effects of aqueous and hydro-ethanolic fractions of leaves of S.senegalensis on Caenorhabditis elegans worm viability were carried out by using both 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-tetrazolium bromide(MTT)assay and microscope motility observation.Results:Among the aqueous fractions,the aqueous decoction showed an EC_(50)at 0.24 mg/ml and a viability rate of 7.5%at 0.9 mg/ml.In hydro-ethanolic extracts,hydro-ethanolic macerate residual(Fe-3)exhibited a significant effect with an EC_(50)at 0.29 mg/ml and 12.5%of viability at 0.9 mg/ml.HPLC-ESI-MS/MS analysis allowed the identification of twelve compounds.Conclusions:Using the MTT-formazan assay,extracts of S.senegalensis exhibit a moderate anthelmintic effect on C.elegans worms linked to natural antiparasitic molecules.展开更多
基金financial support from Campus France(895739H)through the French Embassy in Burkina Faso.
文摘Background:Drug resistance is increasing in gastrointestinal parasitosis;it is therefore essential to identify new anthelmintic molecules.Objective:The study aims to evaluate the anthelmintic activity of extracts and fractions of leaves of Saba sene-galensis(A.DC.)Pichon(Apocynaceae)against L_(3)larvae of Caenorhabditis elegans,a free-living helminth model and to highlight potential anthelmintic molecules by dereplication strategy of molecular networking.Methods:The methanolic extract,ethanolic extract and aqueous extract of the plant’s leaves were carried out,followed by flash chromatography on silica gel for by fractionation,HPLC-MS/MS analysis.The tandem mass data were used for generation of molecular network.The anthelmintic effects of aqueous and hydro-ethanolic fractions of leaves of S.senegalensis on Caenorhabditis elegans worm viability were carried out by using both 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-tetrazolium bromide(MTT)assay and microscope motility observation.Results:Among the aqueous fractions,the aqueous decoction showed an EC_(50)at 0.24 mg/ml and a viability rate of 7.5%at 0.9 mg/ml.In hydro-ethanolic extracts,hydro-ethanolic macerate residual(Fe-3)exhibited a significant effect with an EC_(50)at 0.29 mg/ml and 12.5%of viability at 0.9 mg/ml.HPLC-ESI-MS/MS analysis allowed the identification of twelve compounds.Conclusions:Using the MTT-formazan assay,extracts of S.senegalensis exhibit a moderate anthelmintic effect on C.elegans worms linked to natural antiparasitic molecules.
