Background:Baccaurea motleyana Müll.Arg.,commonly known as Rambai,is a fruit-bearing tree with potential nutraceutical and remedial properties.Traditionally,it is used for postpartum recovery and eye ailments.Obj...Background:Baccaurea motleyana Müll.Arg.,commonly known as Rambai,is a fruit-bearing tree with potential nutraceutical and remedial properties.Traditionally,it is used for postpartum recovery and eye ailments.Objective:This research intended to explore the pharmacological benefits of the methanol extract of Baccaurea motleyana leaves(MEBML)using a comprehensive approach.Methods:The efficacy of the methanol extract of Baccaurea motleyana leaves(MEBML)at 200 mg/kg and 400 mg/kg was evaluated through various in vivo models,including antipyretic testing in Swiss albino mice,analgesic effects via acetic acid-induced writhing and formalin-induced paw licking,and antidiarrheal activity through castor oil-induced diarrhea and gastrointestinal motility tests.Additionally,in vitro cytotoxic effects were assessed using the brine shrimp lethality bioassay,while computational analysis explored interactions between MEBML phytochemicals and proteins related to target diseases.Results:The methanol extract of Baccaurea motleyana leaves(MEBML)demonstrated a significant reduction in yeast-induced pyrexia in Swiss albino mice.It also exhibited a dose-dependent inhibition in both the acetic acid-induced writhing and formalin-induced paw-licking tests,highlighting its analgesic potential.MEBML showed noteworthy antidiarrheal activity in the castor oil-induced diarrhea model and displayed a cytotoxic effect with an LC_(50) value of 125.6μg/ml.Furthermore,phytochemicals from MEBML exhibited strong binding affinities,ranging from-6.1 kcal/mol to-7.3 kcal/mol,with key drug target receptors.These include cyclooxygenase-1(PDB ID:2OYE)and cyclooxygenase-2(PDB ID:6COX)for analgesic and antipyretic activities,the M3 muscarinic cholinergic receptor(PDB ID:5ZHP)for antidiarrheal effects,and human cytochrome P4502C9(PDB ID:1OG5)for cytotoxic evaluations.Conclusion:In conclusion,MEBML showcased substantial pharmacological activity in pain relief,antipyretic ef-fects,antidiarrheal action,and cytotoxicity in diverse experimental systems,indicating its promise as a foundation for novel drug development.展开更多
基金funded and supported by the Na-tional Science and Technology(NST)fellowship program of the Ministry of Science and Technology,Government of the People’s Republic of Bangladesh(Award Id:120005100-3821117).
文摘Background:Baccaurea motleyana Müll.Arg.,commonly known as Rambai,is a fruit-bearing tree with potential nutraceutical and remedial properties.Traditionally,it is used for postpartum recovery and eye ailments.Objective:This research intended to explore the pharmacological benefits of the methanol extract of Baccaurea motleyana leaves(MEBML)using a comprehensive approach.Methods:The efficacy of the methanol extract of Baccaurea motleyana leaves(MEBML)at 200 mg/kg and 400 mg/kg was evaluated through various in vivo models,including antipyretic testing in Swiss albino mice,analgesic effects via acetic acid-induced writhing and formalin-induced paw licking,and antidiarrheal activity through castor oil-induced diarrhea and gastrointestinal motility tests.Additionally,in vitro cytotoxic effects were assessed using the brine shrimp lethality bioassay,while computational analysis explored interactions between MEBML phytochemicals and proteins related to target diseases.Results:The methanol extract of Baccaurea motleyana leaves(MEBML)demonstrated a significant reduction in yeast-induced pyrexia in Swiss albino mice.It also exhibited a dose-dependent inhibition in both the acetic acid-induced writhing and formalin-induced paw-licking tests,highlighting its analgesic potential.MEBML showed noteworthy antidiarrheal activity in the castor oil-induced diarrhea model and displayed a cytotoxic effect with an LC_(50) value of 125.6μg/ml.Furthermore,phytochemicals from MEBML exhibited strong binding affinities,ranging from-6.1 kcal/mol to-7.3 kcal/mol,with key drug target receptors.These include cyclooxygenase-1(PDB ID:2OYE)and cyclooxygenase-2(PDB ID:6COX)for analgesic and antipyretic activities,the M3 muscarinic cholinergic receptor(PDB ID:5ZHP)for antidiarrheal effects,and human cytochrome P4502C9(PDB ID:1OG5)for cytotoxic evaluations.Conclusion:In conclusion,MEBML showcased substantial pharmacological activity in pain relief,antipyretic ef-fects,antidiarrheal action,and cytotoxicity in diverse experimental systems,indicating its promise as a foundation for novel drug development.
