Background:Inflammation is a complex process with significant implications for animal health.Sesamol(SEL),a phenolic lignan from Sesamum indicum L.,possesses various health benefits.Objective:To assess the anti-inflam...Background:Inflammation is a complex process with significant implications for animal health.Sesamol(SEL),a phenolic lignan from Sesamum indicum L.,possesses various health benefits.Objective:To assess the anti-inflammatory effects of SEL alone and in combination with celecoxib(CxB)and ketoprofen(KPN)in a broiler chick model of inflammation,and to explore the molecular interactions of SEL with cyclooxygenase(COx)enzymes using in silico analysis.Methods:Inflammation was induced in broiler chicks via sub-plantar injection of O.5%formaldehyde.Animals were treated orally with SEL(25 mg/kg,50 mg/kg,and 100 mg/kg),CXB(42 mg/kg),KPN(42 mg/kg),or combinations of SEL either with CXB or KPN.Paw licking frequency and edema size were measured.Computational docking studies were performed to investigate SEL's binding affinity to COX-1 and COX-2.Results:SEL significantly(P<0.05)and dose-dependently reduced paw licking frequency and edema size.The combination of SEL with CXB showed a greater anti-inflammatory effect than the combination with KPN.In silico analysis revealed a higher binding affinity of SEL for COx-2(-6.1 kcal/mol)compared to COX-1(-5.8 kcal/mol).Conclusion:SEL exhibits anti-inflammatory activity and demonstrates synergistic effects with CXB and KPN in vivo.These effects are likely mediated through interactions with both COx-1 and COx-2 enzymes,with a preference for COx-2.These findings suggest SEL as a potential therapeutic agent for inflammation.展开更多
Background:Grewia nervosa(Lour.)Panigrahi,a medicinal herb native to the steep forested region of Bangladesh,is utilized as a treatment for a variety of diseases or conditions,including dysmenorrhea,paratyphoid,bone f...Background:Grewia nervosa(Lour.)Panigrahi,a medicinal herb native to the steep forested region of Bangladesh,is utilized as a treatment for a variety of diseases or conditions,including dysmenorrhea,paratyphoid,bone fractures,jaundice,kidney stones,dyspepsia,heat stroke,cold,fever,diarrhea,hepatitis B,ingestion,and anthelmintic medication.This research aims to investigate the phyto-pharmacological qualities of this herb.Methods:The herb was extracted using absolute ethanol followed by fractionation with n-hexane and chloroform.Following an initial investigation of phytochemicals,antioxidant,anti-inflammatory,antimicrobial,and membrane stabilization activities were assessed using the DPPH,egg albumin model,disc diffusion method,and erythrocyte hemolysis model,respectively.Results:A preliminary phytochemical report suggests that G.nervosa contains reducing sugars,gums,amides,alkaloids,glycosides,flavonoids,steroids,tannins,flavonoids,and saponins.In a concentration-dependent approach,its fractional extracts displayed promising antioxidant,anti-inflammatory,antimicrobial,and membrane-stabilizing activity.It was seen that all fractions had a statistically significant capacity to prevent RBCs from hemolyzing,which suggests a potent membrane stabilizing function in-vitro.Conclusion:Taken together,G.nervosa possesses important secondary metabolites and exhibits in-vitro antioxidant,anti-inflammatory,antimicrobial,and membrane stabilizing capacities.This endorses the conventional usage of G.nervosa,although additional study is needed on this potentially beneficial plant.展开更多
文摘Background:Inflammation is a complex process with significant implications for animal health.Sesamol(SEL),a phenolic lignan from Sesamum indicum L.,possesses various health benefits.Objective:To assess the anti-inflammatory effects of SEL alone and in combination with celecoxib(CxB)and ketoprofen(KPN)in a broiler chick model of inflammation,and to explore the molecular interactions of SEL with cyclooxygenase(COx)enzymes using in silico analysis.Methods:Inflammation was induced in broiler chicks via sub-plantar injection of O.5%formaldehyde.Animals were treated orally with SEL(25 mg/kg,50 mg/kg,and 100 mg/kg),CXB(42 mg/kg),KPN(42 mg/kg),or combinations of SEL either with CXB or KPN.Paw licking frequency and edema size were measured.Computational docking studies were performed to investigate SEL's binding affinity to COX-1 and COX-2.Results:SEL significantly(P<0.05)and dose-dependently reduced paw licking frequency and edema size.The combination of SEL with CXB showed a greater anti-inflammatory effect than the combination with KPN.In silico analysis revealed a higher binding affinity of SEL for COx-2(-6.1 kcal/mol)compared to COX-1(-5.8 kcal/mol).Conclusion:SEL exhibits anti-inflammatory activity and demonstrates synergistic effects with CXB and KPN in vivo.These effects are likely mediated through interactions with both COx-1 and COx-2 enzymes,with a preference for COx-2.These findings suggest SEL as a potential therapeutic agent for inflammation.
文摘Background:Grewia nervosa(Lour.)Panigrahi,a medicinal herb native to the steep forested region of Bangladesh,is utilized as a treatment for a variety of diseases or conditions,including dysmenorrhea,paratyphoid,bone fractures,jaundice,kidney stones,dyspepsia,heat stroke,cold,fever,diarrhea,hepatitis B,ingestion,and anthelmintic medication.This research aims to investigate the phyto-pharmacological qualities of this herb.Methods:The herb was extracted using absolute ethanol followed by fractionation with n-hexane and chloroform.Following an initial investigation of phytochemicals,antioxidant,anti-inflammatory,antimicrobial,and membrane stabilization activities were assessed using the DPPH,egg albumin model,disc diffusion method,and erythrocyte hemolysis model,respectively.Results:A preliminary phytochemical report suggests that G.nervosa contains reducing sugars,gums,amides,alkaloids,glycosides,flavonoids,steroids,tannins,flavonoids,and saponins.In a concentration-dependent approach,its fractional extracts displayed promising antioxidant,anti-inflammatory,antimicrobial,and membrane-stabilizing activity.It was seen that all fractions had a statistically significant capacity to prevent RBCs from hemolyzing,which suggests a potent membrane stabilizing function in-vitro.Conclusion:Taken together,G.nervosa possesses important secondary metabolites and exhibits in-vitro antioxidant,anti-inflammatory,antimicrobial,and membrane stabilizing capacities.This endorses the conventional usage of G.nervosa,although additional study is needed on this potentially beneficial plant.
