Background: Curculigo recurvata(C. recurvata) is an enthnomedicinally important herb reported to have significant medicinal values. The present study aimed to explore the in vivo and in silico anti-nociceptive and ant...Background: Curculigo recurvata(C. recurvata) is an enthnomedicinally important herb reported to have significant medicinal values. The present study aimed to explore the in vivo and in silico anti-nociceptive and anti-diarrheal effects of a C. recurvate rhizome methanol extract(Me-RCR).Methods: The analgesic effects of Me-RCR were assessed using acetic acid-induced writhing and the formalin-induced flicking test. The drugs were administered intraperitoneally(IP) at doses of 200 and 400 mg/kg body weight(bw). Anti-diarrheal activity was evaluated by assessing intestinal motility, hypersecretion, and fecal score in mice at oral doses of 200 and 400 mg/kg·bw. Computer facilitated analyses for anti-nociceptive and anti-diarrheal activities of three isolated compounds from C. recurvata were undertaken to identify the best-fit phytoconstituents.Results: The Me-RCR showed significant(P <.05) peripheral anti-nociception at the highest dose. The extract inhibited both early and late phases of nociception in the formalin-induced writhing test. In the castor oil-induced diarrhoea model, the extract significantly(P <.05) prolonged the onset time of diarrhoea, inhibited percentage of diarrhoea, and decreased both the volume and weight of intestinal contents. Rates of intestinal fluid accumulation inhibition were(33.61 ± 1.00)% and(46.44 ± 0.89)% at Me-RCR doses of 200 and 400 mg/kg·bw, respectively. Moreover, a significant(P <.05) reduction in gastrointestinal motility was observed. An absorption, distribution, metabolism, excretion and/or toxicity(ADME/T) test showed that the selected compounds yielded promising results, satisfying Lipinski's rule of five for predicting drug-like potential. Notably, of the three phytoconstituents curculigine and isocurculigine possessed the highest affinity for the COX-1 and COX-2. Isocurculigine was also identified as the most effective anti-diarrheal compound in the computer-facilitated model.Conclusion: An extract of the plant C. recurvata showed potential analgesic and antidiarrheal activity due to the presence of one or more active secondary metabolite(s).展开更多
Objective:To examine hypoglycemic and antibacterial activity against some Gram-positive and Gram-negative bacteria of organic extracts of four Bangladeshi plants.Methods:An in vivo hypoglycemic effect on mice model wa...Objective:To examine hypoglycemic and antibacterial activity against some Gram-positive and Gram-negative bacteria of organic extracts of four Bangladeshi plants.Methods:An in vivo hypoglycemic effect on mice model was used to check the hypoglycemic effect of four Bangladeshi herbal organic extracts viz.,roots of Curculigo recurvata W.T.Aiton(Satipata)(C.recurvata),leaf of Amorphophallus bulbifer Roxb.(Olkachu)(A.bulbifer),whole plant of Thunbergia grandiflora Roxb.(Nillata)(T.grandiflora)and leaf of Steudnera colocasiifolia K.Koch(Yunnan)(S.colocasiifolia)using glibenclamide as a positive control and water as a negative control.They were also tested for antibacterial activity on three Grampositive and four Gram-negative bacteria by disk diffusion method.C.recurvata,A.bulbifer and T.grandiflora were extracted with methanol and S.colocasiifolia was extracted with ethanol.Results:Among all the plant extract,only ethanol extract of S.colocasiifolia leaves at 800 mg/kg dose significantly(P<0.01)reduced fasting glucose level in normal mice as compared to standard drug glibenclamide(5 mg/kg).Ethanol extract of S.colocasiifolia leaves at 800 mg/kg dose decreased 20.28%of blood glucose level after 2 h of administration in normal mice,where glibenclamide decreased 39.63%.Methanol extract of T.grandiflora didn’t show any zone of inhibition against the tested bacteria,but other three extracts showed a wide range of zone of inhibition.However,none of the extract showed antibacterial activity against all the tested bacteria.Methanol extract of C.recurvata showed maximum zone of inhibition against Bacillus cereus[(10.50±0.50)mm],Salmonella typhi[(16.20±1.26)mm],Escherichia coli[(13.00±1.00)mm]and ethanol extract of S.colocasiifolia showed maximum zone of inhibition against Staphylococcus aureus[(11.20±1.26)mm],Bacillus subtilis[(12.00±0.50)mm],Salmonella paratyphi[(10.80±0.29)mm].Only methanol extract of A.bulbifer showed(8.50±0.50)mm and(7.20±0.76)mm zone of inhibition against Pseudomomas aeruginosa at 1000 and 800μg/disk dose respectively.Conclusions:Through our study,it was found that S.colocasiifolia could be considered as very promising and beneficial hypoglycemic agent.Although C.recurvata and S.colocasiifolia showed comparable high antibacterial activity,further studies should be needed to develop new antibacterial agent from them.S.colocasiifolia may be a potential source for the development of new oral hypoglycemic agent.展开更多
Objective:To estimate the antidepressant,anxiolytic and antinociceptive activities of ethanol extract of Steudnera colocasiifolia K.Koch(S.colocasiifolia)leaves.Methods:Swiss albino mice treated with 1%Tween solution,...Objective:To estimate the antidepressant,anxiolytic and antinociceptive activities of ethanol extract of Steudnera colocasiifolia K.Koch(S.colocasiifolia)leaves.Methods:Swiss albino mice treated with 1%Tween solution,standard drugs and ethanol extract of S.colocasiifolia,respectively,were subjected to the neurological and antinociceptive investigations.The tail suspension test and forced swimming test were used for testing antidepressant activity,where the parameter is the measurement of immobility time.Anxiolytic activity was evaluated by hole board model.Anti-nociceptive potential of the extract was also screened for centrally acting analgesic activity by using formalin induced licking response model and acetic acid induced writhing test was used for testing peripheral analgesic action.Results:Ethanol extract of S.colocasiifolia significantly decreased the period of immobility in both tested models(tail suspension and forced swimming models)of antidepressant activity.In the hole board model,there was a dose dependant(at 100 and 200 mg/kg)and a significant increase in the number of head dipping by comparing with control(1%Tween solution)(P<0.05 and P<0.001).In formalin induced licking model,a significant inhibition of pain compared to standard diclofenac sodium was observed(P<0.05 and P<0.001).In acetic acid induced test,there was a significant reduction of writhing response and pain in mice treated with leaves extract of S.colocasiifolia at 200 mg/kg body weight(P<0.05 and P<0.001).Conclusions:The results proofed the prospective antidepressant,anxiolytic and antinociceptive activities of ethanol extract of S.colocasiifolia leaves.展开更多
基金Center for Research and Publication,Grant/Award Number 180111International Islamic University Chittagong。
文摘Background: Curculigo recurvata(C. recurvata) is an enthnomedicinally important herb reported to have significant medicinal values. The present study aimed to explore the in vivo and in silico anti-nociceptive and anti-diarrheal effects of a C. recurvate rhizome methanol extract(Me-RCR).Methods: The analgesic effects of Me-RCR were assessed using acetic acid-induced writhing and the formalin-induced flicking test. The drugs were administered intraperitoneally(IP) at doses of 200 and 400 mg/kg body weight(bw). Anti-diarrheal activity was evaluated by assessing intestinal motility, hypersecretion, and fecal score in mice at oral doses of 200 and 400 mg/kg·bw. Computer facilitated analyses for anti-nociceptive and anti-diarrheal activities of three isolated compounds from C. recurvata were undertaken to identify the best-fit phytoconstituents.Results: The Me-RCR showed significant(P <.05) peripheral anti-nociception at the highest dose. The extract inhibited both early and late phases of nociception in the formalin-induced writhing test. In the castor oil-induced diarrhoea model, the extract significantly(P <.05) prolonged the onset time of diarrhoea, inhibited percentage of diarrhoea, and decreased both the volume and weight of intestinal contents. Rates of intestinal fluid accumulation inhibition were(33.61 ± 1.00)% and(46.44 ± 0.89)% at Me-RCR doses of 200 and 400 mg/kg·bw, respectively. Moreover, a significant(P <.05) reduction in gastrointestinal motility was observed. An absorption, distribution, metabolism, excretion and/or toxicity(ADME/T) test showed that the selected compounds yielded promising results, satisfying Lipinski's rule of five for predicting drug-like potential. Notably, of the three phytoconstituents curculigine and isocurculigine possessed the highest affinity for the COX-1 and COX-2. Isocurculigine was also identified as the most effective anti-diarrheal compound in the computer-facilitated model.Conclusion: An extract of the plant C. recurvata showed potential analgesic and antidiarrheal activity due to the presence of one or more active secondary metabolite(s).
基金Supported by Department of Pharmacy,International Islamic University Chittagong,Bangladesh with a research grant to accomplish the study(Grant No.Pharm-P&D 71/09-15/09).
文摘Objective:To examine hypoglycemic and antibacterial activity against some Gram-positive and Gram-negative bacteria of organic extracts of four Bangladeshi plants.Methods:An in vivo hypoglycemic effect on mice model was used to check the hypoglycemic effect of four Bangladeshi herbal organic extracts viz.,roots of Curculigo recurvata W.T.Aiton(Satipata)(C.recurvata),leaf of Amorphophallus bulbifer Roxb.(Olkachu)(A.bulbifer),whole plant of Thunbergia grandiflora Roxb.(Nillata)(T.grandiflora)and leaf of Steudnera colocasiifolia K.Koch(Yunnan)(S.colocasiifolia)using glibenclamide as a positive control and water as a negative control.They were also tested for antibacterial activity on three Grampositive and four Gram-negative bacteria by disk diffusion method.C.recurvata,A.bulbifer and T.grandiflora were extracted with methanol and S.colocasiifolia was extracted with ethanol.Results:Among all the plant extract,only ethanol extract of S.colocasiifolia leaves at 800 mg/kg dose significantly(P<0.01)reduced fasting glucose level in normal mice as compared to standard drug glibenclamide(5 mg/kg).Ethanol extract of S.colocasiifolia leaves at 800 mg/kg dose decreased 20.28%of blood glucose level after 2 h of administration in normal mice,where glibenclamide decreased 39.63%.Methanol extract of T.grandiflora didn’t show any zone of inhibition against the tested bacteria,but other three extracts showed a wide range of zone of inhibition.However,none of the extract showed antibacterial activity against all the tested bacteria.Methanol extract of C.recurvata showed maximum zone of inhibition against Bacillus cereus[(10.50±0.50)mm],Salmonella typhi[(16.20±1.26)mm],Escherichia coli[(13.00±1.00)mm]and ethanol extract of S.colocasiifolia showed maximum zone of inhibition against Staphylococcus aureus[(11.20±1.26)mm],Bacillus subtilis[(12.00±0.50)mm],Salmonella paratyphi[(10.80±0.29)mm].Only methanol extract of A.bulbifer showed(8.50±0.50)mm and(7.20±0.76)mm zone of inhibition against Pseudomomas aeruginosa at 1000 and 800μg/disk dose respectively.Conclusions:Through our study,it was found that S.colocasiifolia could be considered as very promising and beneficial hypoglycemic agent.Although C.recurvata and S.colocasiifolia showed comparable high antibacterial activity,further studies should be needed to develop new antibacterial agent from them.S.colocasiifolia may be a potential source for the development of new oral hypoglycemic agent.
基金Supported by a research grant from Managing Committee of the Department of Pharmacy,International Islamic University Chittagong,Bangladesh(Grant No.Pharm-P&D 71/09-15/54).
文摘Objective:To estimate the antidepressant,anxiolytic and antinociceptive activities of ethanol extract of Steudnera colocasiifolia K.Koch(S.colocasiifolia)leaves.Methods:Swiss albino mice treated with 1%Tween solution,standard drugs and ethanol extract of S.colocasiifolia,respectively,were subjected to the neurological and antinociceptive investigations.The tail suspension test and forced swimming test were used for testing antidepressant activity,where the parameter is the measurement of immobility time.Anxiolytic activity was evaluated by hole board model.Anti-nociceptive potential of the extract was also screened for centrally acting analgesic activity by using formalin induced licking response model and acetic acid induced writhing test was used for testing peripheral analgesic action.Results:Ethanol extract of S.colocasiifolia significantly decreased the period of immobility in both tested models(tail suspension and forced swimming models)of antidepressant activity.In the hole board model,there was a dose dependant(at 100 and 200 mg/kg)and a significant increase in the number of head dipping by comparing with control(1%Tween solution)(P<0.05 and P<0.001).In formalin induced licking model,a significant inhibition of pain compared to standard diclofenac sodium was observed(P<0.05 and P<0.001).In acetic acid induced test,there was a significant reduction of writhing response and pain in mice treated with leaves extract of S.colocasiifolia at 200 mg/kg body weight(P<0.05 and P<0.001).Conclusions:The results proofed the prospective antidepressant,anxiolytic and antinociceptive activities of ethanol extract of S.colocasiifolia leaves.
