<span style="font-family:""><span style="font-family:Verdana;">For just about 30 years, researchers have considered the likelihood to utilize </span><span style="font...<span style="font-family:""><span style="font-family:Verdana;">For just about 30 years, researchers have considered the likelihood to utilize </span><span style="font-family:Verdana;">nucleic acids as antiviral therapeutics. In principle, small single-stranded</span><span style="font-family:Verdana;"> nuc</span><span style="font-family:Verdana;">leotide sequence (oligonucleotide) could hybridize to a particular gene or</span><span style="font-family:Verdana;"> mes</span><span style="font-family:Verdana;">senger RNA and diminish transcription or translation, respectively, in this</span><span style="font-family:Verdana;"> manner decreasing the amount of protein that is synthesized. Until now, an incredible number of antisense oligonucleotides, double-stranded oligonucleotides, aptamers, ribozymes, deoxyribozymes, interfering RNAs, chimeric RNA</span></span><span style="font-family:Verdana;">-</span><span style="font-family:""><span style="font-family:Verdana;">DNA molecules, antibody genes has been created artificially and ap</span><span style="font-family:Verdana;">plied effectively for comprehension and manipulating biological processe</span><span style="font-family:Verdana;">s and in clinical preliminaries to treat a variety of diseases. Their versatility and potency make them similarly fit candidates for fighting viral infections. However, troubles with their efficiency, off-target effects, toxicity, delivery, and stability halted the development of nucleic acid-based therapeutics that can be utilized in the clinic. The potential for nucleic acid therapeutic agents is significant and is quite recently beginning to be realized. In this review, we have summarized some of the recent advancements made in the area of nucleic acid based therapeutics and focused on the methods of their delivery and associated challenges.展开更多
Rapid and accurate laboratory diagnosis of SARS-CoV-2 infection is crucial for the management of COVID-19 patients and control of the spread of the virus. At the start of the COVID-19 pandemic, Bangladesh had only one...Rapid and accurate laboratory diagnosis of SARS-CoV-2 infection is crucial for the management of COVID-19 patients and control of the spread of the virus. At the start of the COVID-19 pandemic, Bangladesh had only one government molecular laboratory where real-time RT-PCR would be performed to diagnose SARS-CoV-2 infection. With the increasing number of suspected cases requiring confirmation diagnostic testing, there is a requirement to expand capacity for large-scale testing quickly. The government of Bangladesh established over 100 molecular laboratories within one year to test COVID-19. To expand the testing capacity, the government was compelled to recruit laboratory staff with limited experience and technical expertise, especially in molecular assays, to process specimens, interpret results, troubleshoot. As a result, the risk of diagnostic errors, such as cross-contamination, increased, potentially undermining the efficacy of public health policies, public health response, surveillance programs, and restrictive measures aimed toward containing the outbreak. In this piece, we discuss the different sources of cross-contamination in the COVID-19 RT-PCR laboratories and proffer practical preventive measures to avoid them.展开更多
In total 184 studies, which included thyroid uptake and scintigraphy, were performed in 68 hyperthyroid patients: 67% female and 33% male to investigate their thyroid conditions. The aim of the present study was to il...In total 184 studies, which included thyroid uptake and scintigraphy, were performed in 68 hyperthyroid patients: 67% female and 33% male to investigate their thyroid conditions. The aim of the present study was to illustrate the role of uptake and scintigraphy tests in determining the thyroid status of hyperthyroid patients. The uptake study was performed by oral administration of 100 - 200 μCi of 131I as sodium-iodide and counting the radioactivity at 2 and 24 hrs, whereas thyroid scintigraphy was performed 20 minutes after an intravenous injection of 2 - 4 mCi of 99mTc-pertechnetate. The present results of thyroid uptake and scintigraphy successfully identified the thyroid condition in different states. The present results were also compared with some reported data and found to be fair in good agreement.展开更多
文摘<span style="font-family:""><span style="font-family:Verdana;">For just about 30 years, researchers have considered the likelihood to utilize </span><span style="font-family:Verdana;">nucleic acids as antiviral therapeutics. In principle, small single-stranded</span><span style="font-family:Verdana;"> nuc</span><span style="font-family:Verdana;">leotide sequence (oligonucleotide) could hybridize to a particular gene or</span><span style="font-family:Verdana;"> mes</span><span style="font-family:Verdana;">senger RNA and diminish transcription or translation, respectively, in this</span><span style="font-family:Verdana;"> manner decreasing the amount of protein that is synthesized. Until now, an incredible number of antisense oligonucleotides, double-stranded oligonucleotides, aptamers, ribozymes, deoxyribozymes, interfering RNAs, chimeric RNA</span></span><span style="font-family:Verdana;">-</span><span style="font-family:""><span style="font-family:Verdana;">DNA molecules, antibody genes has been created artificially and ap</span><span style="font-family:Verdana;">plied effectively for comprehension and manipulating biological processe</span><span style="font-family:Verdana;">s and in clinical preliminaries to treat a variety of diseases. Their versatility and potency make them similarly fit candidates for fighting viral infections. However, troubles with their efficiency, off-target effects, toxicity, delivery, and stability halted the development of nucleic acid-based therapeutics that can be utilized in the clinic. The potential for nucleic acid therapeutic agents is significant and is quite recently beginning to be realized. In this review, we have summarized some of the recent advancements made in the area of nucleic acid based therapeutics and focused on the methods of their delivery and associated challenges.
文摘Rapid and accurate laboratory diagnosis of SARS-CoV-2 infection is crucial for the management of COVID-19 patients and control of the spread of the virus. At the start of the COVID-19 pandemic, Bangladesh had only one government molecular laboratory where real-time RT-PCR would be performed to diagnose SARS-CoV-2 infection. With the increasing number of suspected cases requiring confirmation diagnostic testing, there is a requirement to expand capacity for large-scale testing quickly. The government of Bangladesh established over 100 molecular laboratories within one year to test COVID-19. To expand the testing capacity, the government was compelled to recruit laboratory staff with limited experience and technical expertise, especially in molecular assays, to process specimens, interpret results, troubleshoot. As a result, the risk of diagnostic errors, such as cross-contamination, increased, potentially undermining the efficacy of public health policies, public health response, surveillance programs, and restrictive measures aimed toward containing the outbreak. In this piece, we discuss the different sources of cross-contamination in the COVID-19 RT-PCR laboratories and proffer practical preventive measures to avoid them.
文摘In total 184 studies, which included thyroid uptake and scintigraphy, were performed in 68 hyperthyroid patients: 67% female and 33% male to investigate their thyroid conditions. The aim of the present study was to illustrate the role of uptake and scintigraphy tests in determining the thyroid status of hyperthyroid patients. The uptake study was performed by oral administration of 100 - 200 μCi of 131I as sodium-iodide and counting the radioactivity at 2 and 24 hrs, whereas thyroid scintigraphy was performed 20 minutes after an intravenous injection of 2 - 4 mCi of 99mTc-pertechnetate. The present results of thyroid uptake and scintigraphy successfully identified the thyroid condition in different states. The present results were also compared with some reported data and found to be fair in good agreement.
