Fusarium graminearum is a fungal plant pathogen which causes Fusarium head blight(FHB), a devastating diseaseon cereal crops. Here we report that FgPMA1 could be a new target to control FHB by the application of doubl...Fusarium graminearum is a fungal plant pathogen which causes Fusarium head blight(FHB), a devastating diseaseon cereal crops. Here we report that FgPMA1 could be a new target to control FHB by the application of double-stranded RNA(dsRNA) of FgPMA1. FgPMA1 was divided into 6 segments to generated RNA interference(RNAi)constructs(FgPMA1RNAi-1,-2,-3,-4,-5, and-6), and these constructs were transformed in F. graminearum strainPH-1. The expression of FgPMA1 reduced by 18.48, 33.48 and 56.93% in FgPMA1RNAi-1, FgPMA1RNAi-2 and FgPMA1RNAi-5, respectively. FgPMA1RNAi-1,-2, and-5 mutants inhibited fungal development, including mycelium growth, mycelial morphology, asexual and sexual development, and toxin production. The length of lesions on wheat leaves, wheat coleoptiles and wheat ears were shorter after infection with FgPMA1RNAi-1,-2, and-5 mutants thanwild type PH-1. These results showed that three segments(FgPMA1RNAi-1,-2, and-5) exhibited effective silencing effects. After treatment with 25 ng μL^(–1)dsRNA of these segments in vitro, the growth rate of mycelium growth was significant decreased, mycelium became deformed with bulbous structure at the tip, and the mycelium lost the ability to produce conidia in F. graminearum strain PH-1, Fusarium asiacitum strain 2021 and phenamacril-resistant strainYP-1. After application of FgPMA1RNAi-1-dsRNA and FgPMA1RNAi-2-dsRNA to wheat ears, pathogenicity reduced 34.21–35.40%.展开更多
Many fungi are pathogenic on plants and cause significant damage in agriculture and forestry.They are also part of the natural ecosystem and may play a role in regulating plant numbers/density.Morphological identifica...Many fungi are pathogenic on plants and cause significant damage in agriculture and forestry.They are also part of the natural ecosystem and may play a role in regulating plant numbers/density.Morphological identification and analysis of plant pathogenic fungi,while important,is often hampered by the scarcity of discriminatory taxonomic characters and the endophytic or inconspicuous nature of these fungi.Molecular(DNA sequence)data for plant pathogenic fungi have emerged as key information for diagnostic and classification studies,although hampered in part by non-standard laboratory practices and analytical methods.To facilitate current and future research,this study provides phylogenetic synopses for 25 groups of plant pathogenic fungi in the Ascomycota,Basidiomycota,Mucormycotina(Fungi),and Oomycota,using recent molecular data,up-to-date names,and the latest taxonomic insights.Lineagespecific laboratory protocols together with advice on their application,as well as general observations,are also provided.We hope to maintain updated backbone trees of these fungal lineages over time and to publish them jointly as new data emerge.Researchers of plant pathogenic fungi not covered by the present study are invited to join this future effort.Bipolaris,Botryosphaeriaceae,Botryosphaeria,Botrytis,Choanephora,Colletotrichum,Curvularia,Diaporthe,Diplodia,Dothiorella,Fusarium,Gilbertella,Lasiodiplodia,Mucor,Neofusicoccum,Pestalotiopsis,Phyllosticta,Phytophthora,Puccinia,Pyrenophora,Pythium,Rhizopus,Stagonosporopsis,Ustilago and Verticillium are dealt with in this paper.展开更多
基金sponsored by the National Natural Science Foundation of China (32272585)the National Key R&D Program of China (2022YFD1400900)the Fundamental Research Funds for the Central Universities, China (KYCXJC2023003)。
文摘Fusarium graminearum is a fungal plant pathogen which causes Fusarium head blight(FHB), a devastating diseaseon cereal crops. Here we report that FgPMA1 could be a new target to control FHB by the application of double-stranded RNA(dsRNA) of FgPMA1. FgPMA1 was divided into 6 segments to generated RNA interference(RNAi)constructs(FgPMA1RNAi-1,-2,-3,-4,-5, and-6), and these constructs were transformed in F. graminearum strainPH-1. The expression of FgPMA1 reduced by 18.48, 33.48 and 56.93% in FgPMA1RNAi-1, FgPMA1RNAi-2 and FgPMA1RNAi-5, respectively. FgPMA1RNAi-1,-2, and-5 mutants inhibited fungal development, including mycelium growth, mycelial morphology, asexual and sexual development, and toxin production. The length of lesions on wheat leaves, wheat coleoptiles and wheat ears were shorter after infection with FgPMA1RNAi-1,-2, and-5 mutants thanwild type PH-1. These results showed that three segments(FgPMA1RNAi-1,-2, and-5) exhibited effective silencing effects. After treatment with 25 ng μL^(–1)dsRNA of these segments in vitro, the growth rate of mycelium growth was significant decreased, mycelium became deformed with bulbous structure at the tip, and the mycelium lost the ability to produce conidia in F. graminearum strain PH-1, Fusarium asiacitum strain 2021 and phenamacril-resistant strainYP-1. After application of FgPMA1RNAi-1-dsRNA and FgPMA1RNAi-2-dsRNA to wheat ears, pathogenicity reduced 34.21–35.40%.
基金the Chinese Academy of Sciences,project number 2013T2S0030,for the award of Visiting Professorship for Senior International Scientists at Kunming Institute of Botanythe National Research Council of Thailand(grant for Pestalotiopsis No:55201020008)+3 种基金Mae Fah Luang University(grant for Pestalotiopsis No:55101020004)for financial supportthe University of Malaya for grant number RU006H-2014 entitled“diversity and importance of fungal mangrove disease”Financial support to Julia Pawłowska and Marta Wrzosek was partially provided by the Polish Ministry of Science and Higher Education(MNiSW),grant no.NN303_548839financial support from FORMAS(215-2011-498).
文摘Many fungi are pathogenic on plants and cause significant damage in agriculture and forestry.They are also part of the natural ecosystem and may play a role in regulating plant numbers/density.Morphological identification and analysis of plant pathogenic fungi,while important,is often hampered by the scarcity of discriminatory taxonomic characters and the endophytic or inconspicuous nature of these fungi.Molecular(DNA sequence)data for plant pathogenic fungi have emerged as key information for diagnostic and classification studies,although hampered in part by non-standard laboratory practices and analytical methods.To facilitate current and future research,this study provides phylogenetic synopses for 25 groups of plant pathogenic fungi in the Ascomycota,Basidiomycota,Mucormycotina(Fungi),and Oomycota,using recent molecular data,up-to-date names,and the latest taxonomic insights.Lineagespecific laboratory protocols together with advice on their application,as well as general observations,are also provided.We hope to maintain updated backbone trees of these fungal lineages over time and to publish them jointly as new data emerge.Researchers of plant pathogenic fungi not covered by the present study are invited to join this future effort.Bipolaris,Botryosphaeriaceae,Botryosphaeria,Botrytis,Choanephora,Colletotrichum,Curvularia,Diaporthe,Diplodia,Dothiorella,Fusarium,Gilbertella,Lasiodiplodia,Mucor,Neofusicoccum,Pestalotiopsis,Phyllosticta,Phytophthora,Puccinia,Pyrenophora,Pythium,Rhizopus,Stagonosporopsis,Ustilago and Verticillium are dealt with in this paper.
