This paper reported the synthesis of hexaar-ylbiimidazole-tetraphenylethene(HABI-TPE)conjugated photochromic fluorophore,which simultaneously exhib-ited photochromic property,condensed state enhanced emission and reve...This paper reported the synthesis of hexaar-ylbiimidazole-tetraphenylethene(HABI-TPE)conjugated photochromic fluorophore,which simultaneously exhib-ited photochromic property,condensed state enhanced emission and reversible fluorescence switching.Upon UV irradiation,a green species with a broad absorption band between 550 and 800 nm(the absorption maximum at 697 nm)was observed,which readily faded to colorless in the darkness.HABI-TPE launched strong fluorescence with the maximum emission wavelength at 520580 nm under the excitation with 450-500 nm visible light in condensed state,which is in contrast to nonfluorescence in solution.The maximum emission wavelength in condensed state was dependent of excitation wavelength.More interest-ingly,HABI-TPE exhibited reversible fluorescence switch-ing upon alternating irradiation with blue or near-UV light(wavelength less than 490 nm)and green light(more than 490 nm)in condensed state.Our evaluation demonstrated that HABI-TPE exhibited great photoswitchable fluores-cence,which is a promising photoswitchable fluorophore for localization-based super-resolution microscopy,evi-dencing by resolving nanostructures with sub-100nm resolution in polymethylmethacrylate films.展开更多
文摘This paper reported the synthesis of hexaar-ylbiimidazole-tetraphenylethene(HABI-TPE)conjugated photochromic fluorophore,which simultaneously exhib-ited photochromic property,condensed state enhanced emission and reversible fluorescence switching.Upon UV irradiation,a green species with a broad absorption band between 550 and 800 nm(the absorption maximum at 697 nm)was observed,which readily faded to colorless in the darkness.HABI-TPE launched strong fluorescence with the maximum emission wavelength at 520580 nm under the excitation with 450-500 nm visible light in condensed state,which is in contrast to nonfluorescence in solution.The maximum emission wavelength in condensed state was dependent of excitation wavelength.More interest-ingly,HABI-TPE exhibited reversible fluorescence switch-ing upon alternating irradiation with blue or near-UV light(wavelength less than 490 nm)and green light(more than 490 nm)in condensed state.Our evaluation demonstrated that HABI-TPE exhibited great photoswitchable fluores-cence,which is a promising photoswitchable fluorophore for localization-based super-resolution microscopy,evi-dencing by resolving nanostructures with sub-100nm resolution in polymethylmethacrylate films.
