We present a wearable microfluidic impedance cytometer implemented on a flexible circuit wristband with on-line smartphone readout for portable biomarker counting and analysis.The platform contains a standard polydime...We present a wearable microfluidic impedance cytometer implemented on a flexible circuit wristband with on-line smartphone readout for portable biomarker counting and analysis.The platform contains a standard polydimethylsiloxane(PDMS)microfluidic channel integrated on a wristband,and the circuitry on the wristband is composed of a custom analog lock-in amplification system,a microcontroller with an 8-bit analog-to-digital converter(ADC),and a Bluetooth module wirelessly paired with a smartphone.The lock-in amplification(LIA)system is implemented with a novel architecture which consists of the lock-in amplifier followed by a high-pass filter stage with DC offset subtraction,and a post-subtraction high gain stage enabling detection of particles as small as 2.8μm using the 8-bit ADC.The Android smartphone application was used to initiate the system and for offline data-plotting and peak counting,and supports online data readout,analysis,and file management.The data is exportable to researchers and medical professionals for in-depth analysis and remote health monitoring.The system,including the microfluidic sensor,microcontroller,and Bluetooth module all fit on the wristband with a footprint of less than 80 cm2.We demonstrate the ability of the system to obtain generalized blood cell counts;however the system can be applied to a wide variety of biomarkers by interchanging the standard microfluidic channel with microfluidic channels designed for biomarker isolation.展开更多
基金This work was partially funded by the National Science Foundation Instrumentation Development for Biological Research Grant award number 1556253also partially by the PhRMA foundation.
文摘We present a wearable microfluidic impedance cytometer implemented on a flexible circuit wristband with on-line smartphone readout for portable biomarker counting and analysis.The platform contains a standard polydimethylsiloxane(PDMS)microfluidic channel integrated on a wristband,and the circuitry on the wristband is composed of a custom analog lock-in amplification system,a microcontroller with an 8-bit analog-to-digital converter(ADC),and a Bluetooth module wirelessly paired with a smartphone.The lock-in amplification(LIA)system is implemented with a novel architecture which consists of the lock-in amplifier followed by a high-pass filter stage with DC offset subtraction,and a post-subtraction high gain stage enabling detection of particles as small as 2.8μm using the 8-bit ADC.The Android smartphone application was used to initiate the system and for offline data-plotting and peak counting,and supports online data readout,analysis,and file management.The data is exportable to researchers and medical professionals for in-depth analysis and remote health monitoring.The system,including the microfluidic sensor,microcontroller,and Bluetooth module all fit on the wristband with a footprint of less than 80 cm2.We demonstrate the ability of the system to obtain generalized blood cell counts;however the system can be applied to a wide variety of biomarkers by interchanging the standard microfluidic channel with microfluidic channels designed for biomarker isolation.
