Agri-food by-products cause significant environmental and annual market losses.This study addresses the challenge of sustainable use of these by-products by developing an innovative food supplement from artichoke resi...Agri-food by-products cause significant environmental and annual market losses.This study addresses the challenge of sustainable use of these by-products by developing an innovative food supplement from artichoke residues,rich in active molecules such as inulin and polyphenols.Its key components were identified and quantified using high-performance liquid chromatography with diode-array detection(HPLC-DAD),loaded into zein liposomes for oral delivery,and evaluated in vitro for biological effects.Zein liposomes were characterized by a small size(≈132 nm),homogeneously dispersion(polydispersity index≈0.19),a negative charge(around-44 mV),and an efficient incorporation of artichoke extract(>70%).Freeze-drying improved the stability of vesicle dispersions and small-Angle X-ray Scattering analysis confirmed that zein stabilized the vesicles under acidic conditions.Zein liposomes controlled the release of loaded molecules in gastrointestinal-simulating pH(at pH 1.20⁓21%of the chlorogenic acid equivalents were released)and inhibited α-amylase(≈~0.90μg of acarbose equivalents/mL)and α-glucosidase(~0.97μg of acarbose equivalents/mL)in vitro.Formulations were biocompatible(cell viability≈90%)with Caco-2 cells and protected them from oxidative stress.Overall,the results suggest that zein liposomes loaded with artichoke by-product extract are well-suited as a food supplement or nutraceutical to boost nutritional value.展开更多
基金the National Recovery and Resilience Plan(NRRP),Mission 4 Component 2 Investment 1.5-Call for tender No.3277 published on December 30,2021,by the Italian Ministry of University and Research(MUR)funded by the European Union-NextGenerationEU,Project Code ECS0000038-Project Title e.INS Ecosystem of Innovation for Next Generation Sardinia-Grant Assignment Decree No.1056 adopted on June 23,2022 and by the Italian Ministry of University and Research(MUR).
文摘Agri-food by-products cause significant environmental and annual market losses.This study addresses the challenge of sustainable use of these by-products by developing an innovative food supplement from artichoke residues,rich in active molecules such as inulin and polyphenols.Its key components were identified and quantified using high-performance liquid chromatography with diode-array detection(HPLC-DAD),loaded into zein liposomes for oral delivery,and evaluated in vitro for biological effects.Zein liposomes were characterized by a small size(≈132 nm),homogeneously dispersion(polydispersity index≈0.19),a negative charge(around-44 mV),and an efficient incorporation of artichoke extract(>70%).Freeze-drying improved the stability of vesicle dispersions and small-Angle X-ray Scattering analysis confirmed that zein stabilized the vesicles under acidic conditions.Zein liposomes controlled the release of loaded molecules in gastrointestinal-simulating pH(at pH 1.20⁓21%of the chlorogenic acid equivalents were released)and inhibited α-amylase(≈~0.90μg of acarbose equivalents/mL)and α-glucosidase(~0.97μg of acarbose equivalents/mL)in vitro.Formulations were biocompatible(cell viability≈90%)with Caco-2 cells and protected them from oxidative stress.Overall,the results suggest that zein liposomes loaded with artichoke by-product extract are well-suited as a food supplement or nutraceutical to boost nutritional value.
