Objective:We conducted a comprehensive physicochemical analysis of one-dimensional ZnO nanowires(1DZnO),incorporating anti-CYFRA 21-1 immobilization to promote fast optical biomarker detection up to 10 ng ml−1.Impact ...Objective:We conducted a comprehensive physicochemical analysis of one-dimensional ZnO nanowires(1DZnO),incorporating anti-CYFRA 21-1 immobilization to promote fast optical biomarker detection up to 10 ng ml−1.Impact Statement:This study highlights the effectiveness of proof-of-concept 1DZnO nanoplatforms for rapid cancer biomarker detection by examining the nanoscale integration of 1DZnO with these bioreceptors to deliver reliable photoluminescent output signals.Introduction:The urgent need for swift and accurate prognoses in healthcare settings drives the rise of sensitive biosensing nanoplatforms for cancer detection,which has benefited from biomarker identification.CYFRA 21-1 is a reliable target for the early prediction of cancer formation that can be perceptible in blood,saliva,and serum.However,1DZnO nanostructures have been barely applied for CYFRA 21-1 detection.Methods:We assessed the nanoscale interaction between 1DZnO and anti-CYFRA 21-1 antibodies to develop rapid CYFRA 21-1 detection in two distinct matrices:PhosphateBuffered Saline(PBS)buffer and artificial saliva.The chemical modifications were tracked utilizing Fourier transform infrared spectroscopy,while transmission electron microscopy and energy dispersive spectroscopy confirmed antigen-antibody interplay over nanostructures.Results:Our results show high antibody immobilization efficiencies,affirming the effectiveness of 1DZnO nanoplatforms for rapid CYFRA 21-1 testing within a 5-min detection window in both PBS and artificial saliva.Photoluminescence measurements also revealed distinct optical responses across biomarker concentrations ranging from 10 to 1,000 ng ml^(−1).Conclusion:Discernible PL signal responses obtained after 5 min affirm the potential of 1DZnO nanoplatforms for further advancement in optical biomarker detection for application in early cancer prognosis.展开更多
基金R.A.S.(CVU 703153)acknowledges the DGAPA-UNAM postdoctoral grant.S.E.M.T.(CVU 703157)acknowledges the DGAPA-UNAM postdoctoral grantP.G.Z.-B.(CVU 787342)acknowledges the CONAHCyT postdoctoral grant received.A.D.+2 种基金G.S.thank the projects PAPIIT-DGAPA-UNAM IA100123 and IG100123The authors also acknowledge the UIC-UNAM joint collaborative project for the supportThis work was supported by DGAPA PAPIIT funding IA100123.
文摘Objective:We conducted a comprehensive physicochemical analysis of one-dimensional ZnO nanowires(1DZnO),incorporating anti-CYFRA 21-1 immobilization to promote fast optical biomarker detection up to 10 ng ml−1.Impact Statement:This study highlights the effectiveness of proof-of-concept 1DZnO nanoplatforms for rapid cancer biomarker detection by examining the nanoscale integration of 1DZnO with these bioreceptors to deliver reliable photoluminescent output signals.Introduction:The urgent need for swift and accurate prognoses in healthcare settings drives the rise of sensitive biosensing nanoplatforms for cancer detection,which has benefited from biomarker identification.CYFRA 21-1 is a reliable target for the early prediction of cancer formation that can be perceptible in blood,saliva,and serum.However,1DZnO nanostructures have been barely applied for CYFRA 21-1 detection.Methods:We assessed the nanoscale interaction between 1DZnO and anti-CYFRA 21-1 antibodies to develop rapid CYFRA 21-1 detection in two distinct matrices:PhosphateBuffered Saline(PBS)buffer and artificial saliva.The chemical modifications were tracked utilizing Fourier transform infrared spectroscopy,while transmission electron microscopy and energy dispersive spectroscopy confirmed antigen-antibody interplay over nanostructures.Results:Our results show high antibody immobilization efficiencies,affirming the effectiveness of 1DZnO nanoplatforms for rapid CYFRA 21-1 testing within a 5-min detection window in both PBS and artificial saliva.Photoluminescence measurements also revealed distinct optical responses across biomarker concentrations ranging from 10 to 1,000 ng ml^(−1).Conclusion:Discernible PL signal responses obtained after 5 min affirm the potential of 1DZnO nanoplatforms for further advancement in optical biomarker detection for application in early cancer prognosis.
