Plant extracts intended for application as natural food antioxidants are largely touted as safe antioxidants without mechanistic interpretation of their in vivo efficacy or tangible proof of their toxicological effect...Plant extracts intended for application as natural food antioxidants are largely touted as safe antioxidants without mechanistic interpretation of their in vivo efficacy or tangible proof of their toxicological effects.The study used RNA-Seq analysis to evaluate the transcriptomic effects of Harpephyllum caffrum Berhn.Fruit peel extracts(HCP)at 250,450 and 650 ppm in Saccharomyces cerevisiae and their ability to attenuate in vivo H_(2)O_(2)-induced oxidative stress in comparison with sodium metabisulphite(SMB)at 450 ppm.The HCP were influenced ribosome biogenesis and spliceosome relative to SMB.Additionally,HCP treatments primed cells for oxidative stress response and recovery with HCP-450 application being optimal,while SMB induced differential upregulation of thioredoxin,peroxiredoxin,glutathione peroxidase and catalase oxidative stress response genes.There were signs of mitochondrial damage in stressed HCP-650,while the proteasomal system and pro-apoptotic genes were induced in the stressed SMB treatment.Overall,the study demonstrates the antioxidant efficacy of HCP,especially at 450 ppm,and its potential to induce epitranscriptomic modifications beneficial for a healthful diet as opposed to SMB.展开更多
基金supported by the South African Research Chairs Initia-tive(SARChI)in Meat Science:Genomics to Nutrinomics and partly funded by the South African Department of Science and Innovation(UID:84633),as directed by the NRF of South AfricaTMP is thankful to the SARChI for bursary support+1 种基金TMP would also like to acknowledge Stellenbosch University Central Analytical Facility(CAF)and Alvera Vorster for performing the RNA-seq workflow and DIPLOMICS for the financial support via the Quality Control training related subsidyThe authors appreciate Dr J.K.Marima for her assistance with analysis and interpretation training.
文摘Plant extracts intended for application as natural food antioxidants are largely touted as safe antioxidants without mechanistic interpretation of their in vivo efficacy or tangible proof of their toxicological effects.The study used RNA-Seq analysis to evaluate the transcriptomic effects of Harpephyllum caffrum Berhn.Fruit peel extracts(HCP)at 250,450 and 650 ppm in Saccharomyces cerevisiae and their ability to attenuate in vivo H_(2)O_(2)-induced oxidative stress in comparison with sodium metabisulphite(SMB)at 450 ppm.The HCP were influenced ribosome biogenesis and spliceosome relative to SMB.Additionally,HCP treatments primed cells for oxidative stress response and recovery with HCP-450 application being optimal,while SMB induced differential upregulation of thioredoxin,peroxiredoxin,glutathione peroxidase and catalase oxidative stress response genes.There were signs of mitochondrial damage in stressed HCP-650,while the proteasomal system and pro-apoptotic genes were induced in the stressed SMB treatment.Overall,the study demonstrates the antioxidant efficacy of HCP,especially at 450 ppm,and its potential to induce epitranscriptomic modifications beneficial for a healthful diet as opposed to SMB.
