Carbohydrate metabolism is critical for male fertility. Carbohydrate reserves (soluble sugars and starch) were quantified in three advanced stages of pollen development, from the final maturation to rehydration, in a ...Carbohydrate metabolism is critical for male fertility. Carbohydrate reserves (soluble sugars and starch) were quantified in three advanced stages of pollen development, from the final maturation to rehydration, in a chili pepper cultivar (Capsicum annuum L. cv. Calypso Red) with partially dehydrated pollen, to define possible carbohydrate inter-conversions. At the same time, the activity of soluble enzymes involved in sucrolysis and amylolysis were quantified to reveal potential metabolic pathways. The carbohydrates found (sucrose, glucose, fructose, and starch) apparently inter-convert. There would be a close relation between enzymatic activities and substrates, and the carbohydrate reserves could be key factors on the regulation of enzymatic activities. All the enzymes tested were active, although the relevance of each one changed along pollen maturation and rehydration, defining different profiles of enzymatic activities for each stage. Some usually neglected enzymes (e.g. soluble neutral invertase) have shown an important role in the stages analyzed, suggesting alternative processes to evaluate in the studies of male fertility control.展开更多
Goji(Lycium barbarum L.)berries are well known for their biological activities and health-promoting antioxidant properties.Environmental and climatic factors can greatly affect accumulation of secondary metabolites,ca...Goji(Lycium barbarum L.)berries are well known for their biological activities and health-promoting antioxidant properties.Environmental and climatic factors can greatly affect accumulation of secondary metabolites,carotenoids and other antioxidants in plants.We determined the content and composition of main functional constituents of goji berries produced in central Italy in relation to the time of harvesting during two ripening seasons by analyzing the main environmental variables involved.The highest flavonoids content(0,19±0,006 mg QE/g)and antioxidant power(0,03±0,001 mmol Fe^(2+)eq/g)were found at the beginning of the fruiting season.In September,berries show the highest total polyphenols(3,22±0,09 mg GAE/g)and zeaxanthin contents(339,79±14,12μg/g).Our study focused on some important aspects related to the production of goji fruits with high nutraceutical characteristics in a non-experimental cultivation.Our results could provide reliable information on Goji performance in the European climate to be used for the exploitation of the species suggesting the best harvesting timing in terms of antioxidant power and nutraceutical features of berries.This information could be particularly useful for farmers eager to improve the quality of their products.展开更多
文摘Carbohydrate metabolism is critical for male fertility. Carbohydrate reserves (soluble sugars and starch) were quantified in three advanced stages of pollen development, from the final maturation to rehydration, in a chili pepper cultivar (Capsicum annuum L. cv. Calypso Red) with partially dehydrated pollen, to define possible carbohydrate inter-conversions. At the same time, the activity of soluble enzymes involved in sucrolysis and amylolysis were quantified to reveal potential metabolic pathways. The carbohydrates found (sucrose, glucose, fructose, and starch) apparently inter-convert. There would be a close relation between enzymatic activities and substrates, and the carbohydrate reserves could be key factors on the regulation of enzymatic activities. All the enzymes tested were active, although the relevance of each one changed along pollen maturation and rehydration, defining different profiles of enzymatic activities for each stage. Some usually neglected enzymes (e.g. soluble neutral invertase) have shown an important role in the stages analyzed, suggesting alternative processes to evaluate in the studies of male fertility control.
文摘Goji(Lycium barbarum L.)berries are well known for their biological activities and health-promoting antioxidant properties.Environmental and climatic factors can greatly affect accumulation of secondary metabolites,carotenoids and other antioxidants in plants.We determined the content and composition of main functional constituents of goji berries produced in central Italy in relation to the time of harvesting during two ripening seasons by analyzing the main environmental variables involved.The highest flavonoids content(0,19±0,006 mg QE/g)and antioxidant power(0,03±0,001 mmol Fe^(2+)eq/g)were found at the beginning of the fruiting season.In September,berries show the highest total polyphenols(3,22±0,09 mg GAE/g)and zeaxanthin contents(339,79±14,12μg/g).Our study focused on some important aspects related to the production of goji fruits with high nutraceutical characteristics in a non-experimental cultivation.Our results could provide reliable information on Goji performance in the European climate to be used for the exploitation of the species suggesting the best harvesting timing in terms of antioxidant power and nutraceutical features of berries.This information could be particularly useful for farmers eager to improve the quality of their products.
