Objective:To survey the prevalence severe diarrhea arising from these bacteria in children under 5 years old in Marvdasht.Methods:In this study faecal sample from 615 children aged <5years old who were hospitalized...Objective:To survey the prevalence severe diarrhea arising from these bacteria in children under 5 years old in Marvdasht.Methods:In this study faecal sample from 615 children aged <5years old who were hospitalized lor gastroenteritis in Fars hospitals in Iran were collected and then enriched in Escherichia coli(E.coli) broth and modified tryplone soy broth with novobiocin media,fermentation of sorbitol,lactose and β— glucoronidase activity of isolated strains was examined by CT—SMAC,VRBA and chromogenic media respectively.Then isolation of E.coli O157:H7 have been confirmed with the use of specific antisera and with multiplex PCR method presence of virulence genes including:xtx_1.stx_2,eae.A.hly has been analyzed.Results:E.coli O157:H7 was detected in 7(1.14%) stool specimens.A significanl difference was seen between detection rale of isolated bacteria from age groups 18-23 months and other age groups(P=0.004).Out of considered virulence genes.only 1 of the isolated strains(0.16%)he stx,and eaeA genes were seen and also all isolated hacleria had resistance to penicillin,ampicillin and erythromycin antibiotics.Conclusions:We found thai children < 2 years of age were at highest risk of infection with E.coli O157:H7.Regarding severity of E.coli O157:H7 pathogenesis,low infectious dose and lack of routine assay for detection ol these bacleria in clinical laboratory,further and completed studies on diagnosis and genolyping of this E.coli O157:H7 strain has been recommended.展开更多
Objective:To evaluate the effects of Pasteurella multocida(P.multocida)vaccines on the expression and release of antibodies,interleukin(IL)-6 and IL-12 by serum.Methods:Balb/c mice were immunized with two formalin and...Objective:To evaluate the effects of Pasteurella multocida(P.multocida)vaccines on the expression and release of antibodies,interleukin(IL)-6 and IL-12 by serum.Methods:Balb/c mice were immunized with two formalin and iron inactivated vaccine doses within 2 weeks.The vaccines were adjuvant with P.multocida A strain,P.multocida B strain and Salmonella typhimurium bacterial DNA(AbDNA,BbDNA and SbDNA for short,respectively).The animals were challenged 4 weeks after immunization.Blood of mice was collected to detect the change of specific antibody,IL-6,and IL-12 using ELISA.Results:The specific antibody and interleukins in the immunized group increased significantly compared to the control mice after vaccination and challenge(P<0.05).The highest release of these cytokines was obtained by P.multocida inactivated with iron and adjuvant with AbDNA at a concentration of 25μg/mL.The antibody titer peak was 0.447 in mice vaccinated with iron-killed whole-cell antigen adjunct with AbDNA.The time-courses of release showed that bacterial DNA was able to stimulate IL-6 and IL-12 production more than alum(P<0.05).Conclusions:Our findings introduce that bacterial DNA is capable of releasing an immunological response with several cytokines.These indicate that bacterial DNA entrapped with killed P.multocida antigen is a new and effective adjuvant to enhance specific immunity and resistance of animal against the infectious pathogen,which could simplify the development of highly promising strong adjuvant.展开更多
基金the Islamic Azad University, Jahrom branch,for their executive support of this project
文摘Objective:To survey the prevalence severe diarrhea arising from these bacteria in children under 5 years old in Marvdasht.Methods:In this study faecal sample from 615 children aged <5years old who were hospitalized lor gastroenteritis in Fars hospitals in Iran were collected and then enriched in Escherichia coli(E.coli) broth and modified tryplone soy broth with novobiocin media,fermentation of sorbitol,lactose and β— glucoronidase activity of isolated strains was examined by CT—SMAC,VRBA and chromogenic media respectively.Then isolation of E.coli O157:H7 have been confirmed with the use of specific antisera and with multiplex PCR method presence of virulence genes including:xtx_1.stx_2,eae.A.hly has been analyzed.Results:E.coli O157:H7 was detected in 7(1.14%) stool specimens.A significanl difference was seen between detection rale of isolated bacteria from age groups 18-23 months and other age groups(P=0.004).Out of considered virulence genes.only 1 of the isolated strains(0.16%)he stx,and eaeA genes were seen and also all isolated hacleria had resistance to penicillin,ampicillin and erythromycin antibiotics.Conclusions:We found thai children < 2 years of age were at highest risk of infection with E.coli O157:H7.Regarding severity of E.coli O157:H7 pathogenesis,low infectious dose and lack of routine assay for detection ol these bacleria in clinical laboratory,further and completed studies on diagnosis and genolyping of this E.coli O157:H7 strain has been recommended.
基金part of the project:Study on immune response pattern of cattle vaccinated by Razi pasteurellosis vaccine(Project number:12-18-18-9458-94014)
文摘Objective:To evaluate the effects of Pasteurella multocida(P.multocida)vaccines on the expression and release of antibodies,interleukin(IL)-6 and IL-12 by serum.Methods:Balb/c mice were immunized with two formalin and iron inactivated vaccine doses within 2 weeks.The vaccines were adjuvant with P.multocida A strain,P.multocida B strain and Salmonella typhimurium bacterial DNA(AbDNA,BbDNA and SbDNA for short,respectively).The animals were challenged 4 weeks after immunization.Blood of mice was collected to detect the change of specific antibody,IL-6,and IL-12 using ELISA.Results:The specific antibody and interleukins in the immunized group increased significantly compared to the control mice after vaccination and challenge(P<0.05).The highest release of these cytokines was obtained by P.multocida inactivated with iron and adjuvant with AbDNA at a concentration of 25μg/mL.The antibody titer peak was 0.447 in mice vaccinated with iron-killed whole-cell antigen adjunct with AbDNA.The time-courses of release showed that bacterial DNA was able to stimulate IL-6 and IL-12 production more than alum(P<0.05).Conclusions:Our findings introduce that bacterial DNA is capable of releasing an immunological response with several cytokines.These indicate that bacterial DNA entrapped with killed P.multocida antigen is a new and effective adjuvant to enhance specific immunity and resistance of animal against the infectious pathogen,which could simplify the development of highly promising strong adjuvant.
