Biochar is a stable carbon substance produced by the pyrolysis of biomass and used as a soil amendment. Biochar application to soil has resulted in agronomic benefits, including improved water-holding capacity. Howeve...Biochar is a stable carbon substance produced by the pyrolysis of biomass and used as a soil amendment. Biochar application to soil has resulted in agronomic benefits, including improved water-holding capacity. However, limited studies exist quantifying different biochars’ roles in soil water retention, especially when the soil is drier than field capacity. The objective of this study was to investigate the effects of poultry litter and woodchip biochars, applied at various rates (i.e., 0, 5, and 10 Mg.ha-1 incorporated to a depth of 10 cm) to a loam soil, on the relationship between soil water potential and water content across a wide range of moisture conditions. Based on analysis of variance, the relationship between water potential and water content differed (P < 0.05) between biochar sources based on differing (P < 0.02) power-function coefficients, with poultry litter biochar having greater water retention capacity across a wide range of water potentials, but was unaffected by application rate. Based on regression analyses, application rate affected the relationship between water potential and water content for poultry litter biochar. Results indicate that biochar may not generally improve water retention at all water contents with one-time application rates. Additional experiments will be necessary to understand the impact on water retention of biochars produced from different feedstocks under varying pyrolysis conditions.展开更多
Broad-host-range plasmids are frequently associated with antibiotic resistance genes and can quickly spread antibiotic resistant phenotypes among diverse bacterial populations. Wastewater treatment plants have been id...Broad-host-range plasmids are frequently associated with antibiotic resistance genes and can quickly spread antibiotic resistant phenotypes among diverse bacterial populations. Wastewater treatment plants have been identified as reservoirs for broad-host-range plasmids carrying resistance genes. The threat of broad-host-range plasmids released into the environment from wastewater treatment plants has identified the need for disinfection protocols to target broad-host- range plasmid destruction. Here we evaluate the efficacy of dissolved ozone at 2 and 8 mg·L–1 as a primary means for the destruction of broad-host-range plasmid and chromosomal DNA in simulated effluent. Pilot-scale tests using an experimental unit were carried out in municipal wastewater treatment plant effluent and compared with ultraviolet (UV)-irradiation and chlorination methodologies. Genes specific to Escherichia coli (uidA) and IncP broad-host-range plasmids (trfA) were monitored using real-time quantitative polymerase chain reaction (qPCR), and total DNA was monitored using absorbance spectroscopy. In wastewater treatment plant experiments, E. coli qPCR results were compared to a recognized culture-based method (Colilert?) for E. coli. In laboratory experiments, dissolved ozone at 8 mg·L–1 significantly destroyed 93% total, 98% E. coli, and 99% of broad-host-range plasmid DNA. Ozonation, UV-irradiation, and chlorination significantly reduced DNA concentrations and culturable E. coli in wastewater treat- ment plant effluent. Chlorination and UV disinfection resulted in 3-log decreases in culture-based E. coli concentrations in wastewater treatment plant effluent while changes were not significant when measured with qPCR. Only ozonation significantly decreased the IncP broad-host-range plasmid trfA gene, although concentrations of 2.2 × 105 copies trfA·L–1 remained in effluent. Disinfection processes utilizing high dissolved ozone concentrations for the destruction of emerging contaminants such as broad-host-range plasmid and total DNA may have utility as methods to ensure downstream environmental health and safe water reuse become more important.展开更多
文摘Biochar is a stable carbon substance produced by the pyrolysis of biomass and used as a soil amendment. Biochar application to soil has resulted in agronomic benefits, including improved water-holding capacity. However, limited studies exist quantifying different biochars’ roles in soil water retention, especially when the soil is drier than field capacity. The objective of this study was to investigate the effects of poultry litter and woodchip biochars, applied at various rates (i.e., 0, 5, and 10 Mg.ha-1 incorporated to a depth of 10 cm) to a loam soil, on the relationship between soil water potential and water content across a wide range of moisture conditions. Based on analysis of variance, the relationship between water potential and water content differed (P < 0.05) between biochar sources based on differing (P < 0.02) power-function coefficients, with poultry litter biochar having greater water retention capacity across a wide range of water potentials, but was unaffected by application rate. Based on regression analyses, application rate affected the relationship between water potential and water content for poultry litter biochar. Results indicate that biochar may not generally improve water retention at all water contents with one-time application rates. Additional experiments will be necessary to understand the impact on water retention of biochars produced from different feedstocks under varying pyrolysis conditions.
文摘Broad-host-range plasmids are frequently associated with antibiotic resistance genes and can quickly spread antibiotic resistant phenotypes among diverse bacterial populations. Wastewater treatment plants have been identified as reservoirs for broad-host-range plasmids carrying resistance genes. The threat of broad-host-range plasmids released into the environment from wastewater treatment plants has identified the need for disinfection protocols to target broad-host- range plasmid destruction. Here we evaluate the efficacy of dissolved ozone at 2 and 8 mg·L–1 as a primary means for the destruction of broad-host-range plasmid and chromosomal DNA in simulated effluent. Pilot-scale tests using an experimental unit were carried out in municipal wastewater treatment plant effluent and compared with ultraviolet (UV)-irradiation and chlorination methodologies. Genes specific to Escherichia coli (uidA) and IncP broad-host-range plasmids (trfA) were monitored using real-time quantitative polymerase chain reaction (qPCR), and total DNA was monitored using absorbance spectroscopy. In wastewater treatment plant experiments, E. coli qPCR results were compared to a recognized culture-based method (Colilert?) for E. coli. In laboratory experiments, dissolved ozone at 8 mg·L–1 significantly destroyed 93% total, 98% E. coli, and 99% of broad-host-range plasmid DNA. Ozonation, UV-irradiation, and chlorination significantly reduced DNA concentrations and culturable E. coli in wastewater treat- ment plant effluent. Chlorination and UV disinfection resulted in 3-log decreases in culture-based E. coli concentrations in wastewater treatment plant effluent while changes were not significant when measured with qPCR. Only ozonation significantly decreased the IncP broad-host-range plasmid trfA gene, although concentrations of 2.2 × 105 copies trfA·L–1 remained in effluent. Disinfection processes utilizing high dissolved ozone concentrations for the destruction of emerging contaminants such as broad-host-range plasmid and total DNA may have utility as methods to ensure downstream environmental health and safe water reuse become more important.
