Vision restoration presents a considerable challenge in the realm of regenerative medicine,while recent progress in ultrasound stimulation has displayed potential as a non-invasive therapeutic approach.This narrative ...Vision restoration presents a considerable challenge in the realm of regenerative medicine,while recent progress in ultrasound stimulation has displayed potential as a non-invasive therapeutic approach.This narrative review offers a comprehensive overview of current research on ultrasound-stimulated neuromodulation,emphasizing its potential as a treatment modality for various nerve injuries.By examining of the efficacy of different types of ultrasound stimulation in modulating peripheral and optic nerves,we can delve into their underlying molecular mechanisms.Furthermore,the review underscores the potential of sonogenetics in vision restoration,which involves leveraging pharmacological and genetic manipulations to inhibit or enhance the expression of related mechanosensitive channels,thereby modulating the strength of the ultrasound response.We also address how methods such as viral transcription can be utilized to render specific neurons or organs highly responsive to ultrasound,leading to significantly improved therapeutic outcomes.展开更多
The purpose of this study is to develop a method for delivering antiinflammatory agents of high molecular weight(e.g.,Avastin)into the posterior segment that does not require injections into the eye(i.e.,intravitreal ...The purpose of this study is to develop a method for delivering antiinflammatory agents of high molecular weight(e.g.,Avastin)into the posterior segment that does not require injections into the eye(i.e.,intravitreal injections;IVT).Diseases affecting the posterior segment of the eye are currently treated with monthly to bimonthly intravitreal injections,which can predispose patients to severe albeit rare complications like endophthalmitis,retinal detachment,traumatic cataract,and/or increased intraocular.In this study,we show that one time moderate intensity focused ultrasound(MIFU)treatment can facilitate the penetration of large molecules across the scleral barrier,showing promising evidence that this is a viable method to deliver high molecular weight medications not invasively.To validate the efficacy of the drug delivery system,IVT injections of vascular endothelial growth factor(VEGF)were used to create an animal model of retinopathy.The creation of this model allowed us to test anti-VEGF medications and evaluate the efficacy of the treatment.In vivo testing showed that animals treated with our MIFU device improved on the retinal tortuosity and clinical dilation compared to the control group while evaluating fluorescein angiogram(FA)Images.展开更多
基金supported by R01EY032229(Biomechanical Mapping of the Optic Nerve Head and Peripapillary Sclera Using High Frequency Ultrasonic Elastography)R01EY028662(High-resolution Elastographic Assessment of the Optic Nerve Head)+2 种基金R01EY030126(Non-invasive Ultrasound Stimulated Retinal Prosthesis)(to QZ)Unrestricted Grant to the Department of Ophthalmology from Research to Prevent Blindness,New York,NY(to MSH)the National Eye Institute of the National Institutes of Health under Award Number P30EY029220(to MSH)。
文摘Vision restoration presents a considerable challenge in the realm of regenerative medicine,while recent progress in ultrasound stimulation has displayed potential as a non-invasive therapeutic approach.This narrative review offers a comprehensive overview of current research on ultrasound-stimulated neuromodulation,emphasizing its potential as a treatment modality for various nerve injuries.By examining of the efficacy of different types of ultrasound stimulation in modulating peripheral and optic nerves,we can delve into their underlying molecular mechanisms.Furthermore,the review underscores the potential of sonogenetics in vision restoration,which involves leveraging pharmacological and genetic manipulations to inhibit or enhance the expression of related mechanosensitive channels,thereby modulating the strength of the ultrasound response.We also address how methods such as viral transcription can be utilized to render specific neurons or organs highly responsive to ultrasound,leading to significantly improved therapeutic outcomes.
基金supported by the USC Steven Technology Advancement grant and the Jean Perkins Foundation Funding.
文摘The purpose of this study is to develop a method for delivering antiinflammatory agents of high molecular weight(e.g.,Avastin)into the posterior segment that does not require injections into the eye(i.e.,intravitreal injections;IVT).Diseases affecting the posterior segment of the eye are currently treated with monthly to bimonthly intravitreal injections,which can predispose patients to severe albeit rare complications like endophthalmitis,retinal detachment,traumatic cataract,and/or increased intraocular.In this study,we show that one time moderate intensity focused ultrasound(MIFU)treatment can facilitate the penetration of large molecules across the scleral barrier,showing promising evidence that this is a viable method to deliver high molecular weight medications not invasively.To validate the efficacy of the drug delivery system,IVT injections of vascular endothelial growth factor(VEGF)were used to create an animal model of retinopathy.The creation of this model allowed us to test anti-VEGF medications and evaluate the efficacy of the treatment.In vivo testing showed that animals treated with our MIFU device improved on the retinal tortuosity and clinical dilation compared to the control group while evaluating fluorescein angiogram(FA)Images.
