This study evaluated the impact of combining ultrasound technology and lactic acid fermentation with potential probiotics Lactiplantibacillus plantarum strains LpAv and 73a on blueberry-watermelon smoothies(BWS),focus...This study evaluated the impact of combining ultrasound technology and lactic acid fermentation with potential probiotics Lactiplantibacillus plantarum strains LpAv and 73a on blueberry-watermelon smoothies(BWS),focusing on microbial spoilage inhibition,lactic acid bacteria(LAB)viability,physicochemical,and bioactive properties.It also analyzed the influence of ultrasonication order relative to fermentation on LAB survival after in vitro digestion,bioaccessibility of phenolic compounds,and cellular anti-inflammatory and antioxidant properties.Combining fermentation and ultrasonication synergistically inhibited spoilage microbial growth,regardless of the order of ultrasonication.However,ultrasonication after fermentation decreased LAB viability from 9 to 7 log colony forming units(CFU)/mL.LpAv and 73a strains showed strong survival through digestion,maintaining levels above 6 log CFU/mL during the intestinal phase.Fermentation and ultrasonication of BWS reduced antioxidant activity and phenolic content but enhanced post-digestion recovery,with the bioaccessibility of certain phenolics,like(+)-catechin,significantly improved by ultrasonication(40-65%)compared to nonultrasonicated BWS(15-25%).Digested BWS exhibited significant anti-inflammatory effects,reducing nitric oxide and pro-inflammatory cytokines such as IL-1βand TNF-α,with elevated levels of the anti-inflammatory cytokine IL-10 in BWS fermented with the LpAv strain and ultrasonicated after fermentation.A preliminary sensory evaluation indicated that this formulation also achieved good consumer acceptability,particularly in texture and overall liking.The combined effects of lactic acid fermentation and ultrasonication on BWS highlight their promising applications for improving microbial safety and health benefits in functional fruit smoothies.展开更多
Research into natural sources of molecules that can modulate the immune system has gained significant interest in recent years,with a special emphasis on plant-based alternatives that could lead to the development of ...Research into natural sources of molecules that can modulate the immune system has gained significant interest in recent years,with a special emphasis on plant-based alternatives that could lead to the development of immunoceuticals.This study focused on the evaluation of the immunomodulatory potential of extracts obtained from three Opuntia varieties abundant in betalains and phenolic compounds:Opuntia stricta(var.dillenii)whole fruit extract(OD),Opuntia ficus-indica var.Blanca peel extract(BB)and Opuntia ficus-indica var.Colorada pulp extract(COL).All three extracts from these Opuntia varieties demonstrated significant antioxidant activity in Caco-2 and HepG2 cells and stimulated phagocytosis in RAW 264.7 macrophages.Among them,OD extract exhibited the most diverse composition in betalains and phenolic compounds,and the highest immunomodulatory potential by highly inhibiting nitric oxide(NO)production and enhancing the phagocytic activity in RAW 264.7 macrophages.Consequently,its composition was fractionated by Fast Centrifugal Partition Chromatography(FCPC)to conduct a bioguided study of the compound or groups of compounds responsible for the evaluated biological activities.A total of 12 fractions were obtained(F1-F12).Overall,fractions F3,F6,and,F7 showed greater activity in modulating cytokine production and inhibition,particularly tumoral necrosis factor(TNF)-α,and interleukins(IL-6,IL-10,and IL-1β),while the whole OD extract was more effective at reducing NO production and stimulating phagocytosis.These results underscore the potential of Opuntia cactus fruits as promising candidates for the development of natural immunomodulatory agents,widening the way for future research into their application as effective immunoceuticals.展开更多
基金supported by Secretaría de Innovacion,Ciencia y Tecnología de Jalisco,Consejo Estatal de Ciencia y Tecnología de Jalisco(COECYTJAL)Tecnologico de Monterrey(Projects:FODECIJAL 9748-2021 and FODECIJAL 10636-2023)Challenge-Based Research Funding Program 2022 from Tecnologico de Monterrey(Project E071-EIC-GI02-A-T11-D)。
文摘This study evaluated the impact of combining ultrasound technology and lactic acid fermentation with potential probiotics Lactiplantibacillus plantarum strains LpAv and 73a on blueberry-watermelon smoothies(BWS),focusing on microbial spoilage inhibition,lactic acid bacteria(LAB)viability,physicochemical,and bioactive properties.It also analyzed the influence of ultrasonication order relative to fermentation on LAB survival after in vitro digestion,bioaccessibility of phenolic compounds,and cellular anti-inflammatory and antioxidant properties.Combining fermentation and ultrasonication synergistically inhibited spoilage microbial growth,regardless of the order of ultrasonication.However,ultrasonication after fermentation decreased LAB viability from 9 to 7 log colony forming units(CFU)/mL.LpAv and 73a strains showed strong survival through digestion,maintaining levels above 6 log CFU/mL during the intestinal phase.Fermentation and ultrasonication of BWS reduced antioxidant activity and phenolic content but enhanced post-digestion recovery,with the bioaccessibility of certain phenolics,like(+)-catechin,significantly improved by ultrasonication(40-65%)compared to nonultrasonicated BWS(15-25%).Digested BWS exhibited significant anti-inflammatory effects,reducing nitric oxide and pro-inflammatory cytokines such as IL-1βand TNF-α,with elevated levels of the anti-inflammatory cytokine IL-10 in BWS fermented with the LpAv strain and ultrasonicated after fermentation.A preliminary sensory evaluation indicated that this formulation also achieved good consumer acceptability,particularly in texture and overall liking.The combined effects of lactic acid fermentation and ultrasonication on BWS highlight their promising applications for improving microbial safety and health benefits in functional fruit smoothies.
基金funded by project PID2020-118300RB-C21 from MCIN/AEI/10.13039/501100011033“ERDF A way of making Europe”+1 种基金co-supported by the Challenge-Based Research Funding Program I007 IOR001-C5-T1-T from the Institute for Obesity Research of Tecnológico de MonterreySara Parralejo-Sanz thanks the Spanish National Research Council(CSIC)for the iMOVE grant for her PhD internationalization.
文摘Research into natural sources of molecules that can modulate the immune system has gained significant interest in recent years,with a special emphasis on plant-based alternatives that could lead to the development of immunoceuticals.This study focused on the evaluation of the immunomodulatory potential of extracts obtained from three Opuntia varieties abundant in betalains and phenolic compounds:Opuntia stricta(var.dillenii)whole fruit extract(OD),Opuntia ficus-indica var.Blanca peel extract(BB)and Opuntia ficus-indica var.Colorada pulp extract(COL).All three extracts from these Opuntia varieties demonstrated significant antioxidant activity in Caco-2 and HepG2 cells and stimulated phagocytosis in RAW 264.7 macrophages.Among them,OD extract exhibited the most diverse composition in betalains and phenolic compounds,and the highest immunomodulatory potential by highly inhibiting nitric oxide(NO)production and enhancing the phagocytic activity in RAW 264.7 macrophages.Consequently,its composition was fractionated by Fast Centrifugal Partition Chromatography(FCPC)to conduct a bioguided study of the compound or groups of compounds responsible for the evaluated biological activities.A total of 12 fractions were obtained(F1-F12).Overall,fractions F3,F6,and,F7 showed greater activity in modulating cytokine production and inhibition,particularly tumoral necrosis factor(TNF)-α,and interleukins(IL-6,IL-10,and IL-1β),while the whole OD extract was more effective at reducing NO production and stimulating phagocytosis.These results underscore the potential of Opuntia cactus fruits as promising candidates for the development of natural immunomodulatory agents,widening the way for future research into their application as effective immunoceuticals.
