Objective: To investigate the antifungal activity of the fern species Lygodium venustum(L. venustum) and Pityrogramma calomelanos(P. calomelanos) against Candida albicans and Candida tropicalis strains.Methods: The mi...Objective: To investigate the antifungal activity of the fern species Lygodium venustum(L. venustum) and Pityrogramma calomelanos(P. calomelanos) against Candida albicans and Candida tropicalis strains.Methods: The microdilution method was used to evaluate the antifungal activity, as well as the modulating effects of ethanolic extracts of these plants in combination with fluconazole. The minimum inhibitory concentration(MIC), minimum fungicide concentration and morphological changes were also determined.Results: The extract obtained from L. venustum presented a MIC > 8 192 mg/m L, while the extract obtained from and P. calomelanos presented a MIC = 8 192 mg/m L, indicating that they present weak antifungal activity. However, combination of the extracts with Fluconazole potentiated the antifungal activity of this drug. At different experimental conditions, such as concentration of the extract and type of strain, the extracts inhibited hyphae and pseudohyphae formation, indicating that these fern species can affect the morphology of the fungi.Conclusions: The extracts obtained from the fern species L. venustum and P. calomelanos dose not present significant antifungal activity. However, P. calomelanos potentiates the activity of fluconazole and both extracts inhibits the morphological changes in Candida species, indicating that they have potential pharmacological activity as modulators of fungal biology. Therefore, novel studies are required to characterize the interference of these extracts in the virulence and pathogenicity of Candida species as well as the potential of fern species to treat fungal infections.展开更多
The present study aims to demonstrate the chemical composition of the ethanolic extract of Sarcomphalus joazeiro bark and to evaluate its antibacterial and antibiofilm activity.For that,the ethanolic extract of the st...The present study aims to demonstrate the chemical composition of the ethanolic extract of Sarcomphalus joazeiro bark and to evaluate its antibacterial and antibiofilm activity.For that,the ethanolic extract of the stem bark was prepared,and chemical characterization was carried out in UPLC-ESI-QToF-MSE.Minimum inhibitory concentration and antibiotic modification test were performed by the microdilution method.Formation and antiformation assays were evaluated by the crystal violet method.In the chemical characterization,the presence of 24 peaks was observed.Antibacterial activity showed minimum inhibitory concentration≥2048μg/mL.In the evaluations of the potentiating activity,synergism was the most evident.In the formation analysis,it was observed that most strains showed moderate biofilm formation.It was concluded that saponin derivatives are the major secondary metabolites in the analyzed extract and that it presents relevant synergism with the antibiotics studied and a significant ability to inhibit bacterial biofilm formation.展开更多
文摘Objective: To investigate the antifungal activity of the fern species Lygodium venustum(L. venustum) and Pityrogramma calomelanos(P. calomelanos) against Candida albicans and Candida tropicalis strains.Methods: The microdilution method was used to evaluate the antifungal activity, as well as the modulating effects of ethanolic extracts of these plants in combination with fluconazole. The minimum inhibitory concentration(MIC), minimum fungicide concentration and morphological changes were also determined.Results: The extract obtained from L. venustum presented a MIC > 8 192 mg/m L, while the extract obtained from and P. calomelanos presented a MIC = 8 192 mg/m L, indicating that they present weak antifungal activity. However, combination of the extracts with Fluconazole potentiated the antifungal activity of this drug. At different experimental conditions, such as concentration of the extract and type of strain, the extracts inhibited hyphae and pseudohyphae formation, indicating that these fern species can affect the morphology of the fungi.Conclusions: The extracts obtained from the fern species L. venustum and P. calomelanos dose not present significant antifungal activity. However, P. calomelanos potentiates the activity of fluconazole and both extracts inhibits the morphological changes in Candida species, indicating that they have potential pharmacological activity as modulators of fungal biology. Therefore, novel studies are required to characterize the interference of these extracts in the virulence and pathogenicity of Candida species as well as the potential of fern species to treat fungal infections.
基金Microbiology and Molecular Biology Laboratory of the Regional University of Cariri(URCA)Project:Natural Projects as a Control Strategy against Bacterial Biofilms Public Notice/Call:Research Productivity Grant,Stimulation of Internalization and Technological Innovation-BPI 02/2022,number BP4-0172-00215.01.00/20 SPU n:09685010/2020.
文摘The present study aims to demonstrate the chemical composition of the ethanolic extract of Sarcomphalus joazeiro bark and to evaluate its antibacterial and antibiofilm activity.For that,the ethanolic extract of the stem bark was prepared,and chemical characterization was carried out in UPLC-ESI-QToF-MSE.Minimum inhibitory concentration and antibiotic modification test were performed by the microdilution method.Formation and antiformation assays were evaluated by the crystal violet method.In the chemical characterization,the presence of 24 peaks was observed.Antibacterial activity showed minimum inhibitory concentration≥2048μg/mL.In the evaluations of the potentiating activity,synergism was the most evident.In the formation analysis,it was observed that most strains showed moderate biofilm formation.It was concluded that saponin derivatives are the major secondary metabolites in the analyzed extract and that it presents relevant synergism with the antibiotics studied and a significant ability to inhibit bacterial biofilm formation.
