A multifunctional trastuzumab-nanoparticle-fluorocarbon system was developed to maximize the diagnostic effects in human epidermal growth factor receptor 2(HER2)-positive breast cancer.The mesoporous silica nanopartic...A multifunctional trastuzumab-nanoparticle-fluorocarbon system was developed to maximize the diagnostic effects in human epidermal growth factor receptor 2(HER2)-positive breast cancer.The mesoporous silica nanoparticle shape(e.g.amorphous,spherical,and tubular)was altered to optimize the ultrasound contrast potential.Fluorocarbon conjugated mesoporous silica nanoparticles produced higher mean pixel intensities.At lower non-toxic concentrations,tubular shaped nanoparticles produced a higher mean pixel intensity compared to amorphous and spherical particles.All systems displayed a clear binding preference towards HER2-positive breast cancer cells.Increased incubation times and conjugation of fluorocarbon to mesoporous nanoparticles increased binding preference to HER2-positive breast cancer cells.The highest binding affinity was seen with tubular shaped nanoparticles as compared to amorphous and spherical particles.The trastuzumab-nanoparticlefluorocarbon system of each morphology displayed functionality of enhancing contrast in ultrasound.展开更多
基金supported by University of Colorado Denver Start-up funding for Dr.Park.
文摘A multifunctional trastuzumab-nanoparticle-fluorocarbon system was developed to maximize the diagnostic effects in human epidermal growth factor receptor 2(HER2)-positive breast cancer.The mesoporous silica nanoparticle shape(e.g.amorphous,spherical,and tubular)was altered to optimize the ultrasound contrast potential.Fluorocarbon conjugated mesoporous silica nanoparticles produced higher mean pixel intensities.At lower non-toxic concentrations,tubular shaped nanoparticles produced a higher mean pixel intensity compared to amorphous and spherical particles.All systems displayed a clear binding preference towards HER2-positive breast cancer cells.Increased incubation times and conjugation of fluorocarbon to mesoporous nanoparticles increased binding preference to HER2-positive breast cancer cells.The highest binding affinity was seen with tubular shaped nanoparticles as compared to amorphous and spherical particles.The trastuzumab-nanoparticlefluorocarbon system of each morphology displayed functionality of enhancing contrast in ultrasound.
