AIM： To define the frequency of the cfr-13910 variant associated with lactase persistence/non-persistence trait and to analyze the milk consumption of lactase non- persistent subjects in Estonia. METHODS： We genotyp...AIM： To define the frequency of the cfr-13910 variant associated with lactase persistence/non-persistence trait and to analyze the milk consumption of lactase non- persistent subjects in Estonia. METHODS： We genotyped 355 Estonians by polymerase chain reaction and direct sequencing, Milk consumption was analyzed by a questionnaire, specially developed to analyze milk consumption and abdominal complaints. RESULTS： The frequency of the genotype of the C/ C-13910 （lactase non-persistence） was found to be 24.8% in native Estonians. No other single nucleotide polymorphisms covering the region of 400 bp adjacent to the C/T-13910 variant were found. Lactase non- persistence subjects were found to consume less milk than lactase persistence subjects. CONCLUSION： The frequency of lactase non- persistence defined by the C/C-13910 genotype confirms the results of the previous studies based on indirect methods of determining hypolactasia, Milk consumption of lactase non-persistence subjects is consistent with previously reported figures of adult-type hypolactasia in Estonia, However, lactase non-persistence does not prevent the intake of milk in many adults.展开更多
The aim of this investigation was to analyze the associations of TMJ pain/discomfort and bone characteristics on the activities of daily living in a population based study. Methods: The study included 95 randomly sele...The aim of this investigation was to analyze the associations of TMJ pain/discomfort and bone characteristics on the activities of daily living in a population based study. Methods: The study included 95 randomly selected participants. The impact of temporomandibular joint pain/discomfort on the daily activeties was assessed by using the daily living scale by List and Helkimo (1995). The levels of serum type I collagen telopeptide fragments (P1NP), C-telopeptide crosslaps of type I collagen (CTX-1), and vitamin D (25 (OH) D) were analysed. The Mann-Whitney U-test, Spearman rank correlation coefficent (rs) were used for statistical analyses. Results: Activities of daily living were influenced by temporomandibular joint pain/discomfort to varying degrees. The degree of pain/discomfort during eating was correlated with the level of C-telopeptide crosslaps of type I collagen (p = 0.02) and type 1 collagen telopeptide fragments (p = 0.04). The females in the study had significantly lower vitamin D levels compared to males (p = 0.04). Conclusions: This population based study indicates that TMJ pain/discomfort exerts a significant negative influence on activities of daily living and is related to the biochemical markers of bone turnover and 25 (OH) D level. Several functions of daily activities such as social life, exercising and eating are the most disturbed.展开更多
基金Supported by the Estonian Science Foundation grant No. 6452, Sigrid Jusélius Foundation, Helsinki, Finland and Helsinki University Hospital Research Funding, University of Helsinki, Finland
文摘AIM： To define the frequency of the cfr-13910 variant associated with lactase persistence/non-persistence trait and to analyze the milk consumption of lactase non- persistent subjects in Estonia. METHODS： We genotyped 355 Estonians by polymerase chain reaction and direct sequencing, Milk consumption was analyzed by a questionnaire, specially developed to analyze milk consumption and abdominal complaints. RESULTS： The frequency of the genotype of the C/ C-13910 （lactase non-persistence） was found to be 24.8% in native Estonians. No other single nucleotide polymorphisms covering the region of 400 bp adjacent to the C/T-13910 variant were found. Lactase non- persistence subjects were found to consume less milk than lactase persistence subjects. CONCLUSION： The frequency of lactase non- persistence defined by the C/C-13910 genotype confirms the results of the previous studies based on indirect methods of determining hypolactasia, Milk consumption of lactase non-persistence subjects is consistent with previously reported figures of adult-type hypolactasia in Estonia, However, lactase non-persistence does not prevent the intake of milk in many adults.
文摘The aim of this investigation was to analyze the associations of TMJ pain/discomfort and bone characteristics on the activities of daily living in a population based study. Methods: The study included 95 randomly selected participants. The impact of temporomandibular joint pain/discomfort on the daily activeties was assessed by using the daily living scale by List and Helkimo (1995). The levels of serum type I collagen telopeptide fragments (P1NP), C-telopeptide crosslaps of type I collagen (CTX-1), and vitamin D (25 (OH) D) were analysed. The Mann-Whitney U-test, Spearman rank correlation coefficent (rs) were used for statistical analyses. Results: Activities of daily living were influenced by temporomandibular joint pain/discomfort to varying degrees. The degree of pain/discomfort during eating was correlated with the level of C-telopeptide crosslaps of type I collagen (p = 0.02) and type 1 collagen telopeptide fragments (p = 0.04). The females in the study had significantly lower vitamin D levels compared to males (p = 0.04). Conclusions: This population based study indicates that TMJ pain/discomfort exerts a significant negative influence on activities of daily living and is related to the biochemical markers of bone turnover and 25 (OH) D level. Several functions of daily activities such as social life, exercising and eating are the most disturbed.
