The aim of this research work was to evaluate the potential of Bacillus velezensis for proteases production by employing eggshells and egg membrane powder as substrate followed by statistical optimization via RSM.Whil...The aim of this research work was to evaluate the potential of Bacillus velezensis for proteases production by employing eggshells and egg membrane powder as substrate followed by statistical optimization via RSM.While,optimizing the physical parameters maximum protease production was observed at incubation period of 72-h,inoculum size of 2%and substrate concentration of 1.5%.Optimization of nutritional parameters was done through PBD by running 12 experiments,among 7 screened factors(Glucose,peptone,KH_(2)PO_(4),MgSO_(4),NaCl,CaCl_(2)and yeast extract),2 factors(Glucose and CaCl_(2))was found significant.Significant factors were further optimized through CCD and statistical analysis of results were also performed by running 2-Factor ANOVA.Maximum protease activity of 301.0010 U/mlU/ml was observed.Then,enzyme characterization revealed maximum protease activity at 45˚C,pH-8,with highest thermal stability at 60˚C.Furthermore,effects of metal ions(Ca^(+2),Cu^(+2),Mn^(+2),Mg^(+2),K^(+),Na^(+))on protease activity was also evaluated and results have shown Cu^(+2)ions increase protease activity up-to 100-folds while Na^(+),Mg^(+2)and Mn^(+2)ions enhanced protease activity by 103.40,115.42 and 106.01%respectively.The produced protease could be investigated further for potential application as excellent stain remover.展开更多
Glucose oxidase(GOx)is a well-known oxidoreductase enzyme that catalyzes the oxidation ofβ-D glucose into d-gluconic acid in the presence of oxygen as an electron acceptor mediator.It results in the release of hydrog...Glucose oxidase(GOx)is a well-known oxidoreductase enzyme that catalyzes the oxidation ofβ-D glucose into d-gluconic acid in the presence of oxygen as an electron acceptor mediator.It results in the release of hydrogen peroxide(H2O2)as a by-product.Although different bacteria and insects naturally produce the enzyme,fungi are the primary sources.GOx is a valuable enzyme in the food and textile industry,medical diagnostics,and biofuel cell improvement.Recent advances in nanotechnology have shown the great significance of GOx in biosensors,targeted drug delivery,bio-catalysis,and cancer treatment.This review aims to provide a comprehensive summary of the structure,production,and optimization strategies regarding GOx.The analysis of various aspects of GOx is crucial for further modification of enzymes to meet emerging applications in multiple fields.展开更多
文摘The aim of this research work was to evaluate the potential of Bacillus velezensis for proteases production by employing eggshells and egg membrane powder as substrate followed by statistical optimization via RSM.While,optimizing the physical parameters maximum protease production was observed at incubation period of 72-h,inoculum size of 2%and substrate concentration of 1.5%.Optimization of nutritional parameters was done through PBD by running 12 experiments,among 7 screened factors(Glucose,peptone,KH_(2)PO_(4),MgSO_(4),NaCl,CaCl_(2)and yeast extract),2 factors(Glucose and CaCl_(2))was found significant.Significant factors were further optimized through CCD and statistical analysis of results were also performed by running 2-Factor ANOVA.Maximum protease activity of 301.0010 U/mlU/ml was observed.Then,enzyme characterization revealed maximum protease activity at 45˚C,pH-8,with highest thermal stability at 60˚C.Furthermore,effects of metal ions(Ca^(+2),Cu^(+2),Mn^(+2),Mg^(+2),K^(+),Na^(+))on protease activity was also evaluated and results have shown Cu^(+2)ions increase protease activity up-to 100-folds while Na^(+),Mg^(+2)and Mn^(+2)ions enhanced protease activity by 103.40,115.42 and 106.01%respectively.The produced protease could be investigated further for potential application as excellent stain remover.
文摘Glucose oxidase(GOx)is a well-known oxidoreductase enzyme that catalyzes the oxidation ofβ-D glucose into d-gluconic acid in the presence of oxygen as an electron acceptor mediator.It results in the release of hydrogen peroxide(H2O2)as a by-product.Although different bacteria and insects naturally produce the enzyme,fungi are the primary sources.GOx is a valuable enzyme in the food and textile industry,medical diagnostics,and biofuel cell improvement.Recent advances in nanotechnology have shown the great significance of GOx in biosensors,targeted drug delivery,bio-catalysis,and cancer treatment.This review aims to provide a comprehensive summary of the structure,production,and optimization strategies regarding GOx.The analysis of various aspects of GOx is crucial for further modification of enzymes to meet emerging applications in multiple fields.
