黄瓜绿斑驳花叶病毒Cucumber green mottle mosaic virus(CGMMV)是葫芦科作物的重要检疫性病毒。本研究以田间自然感病的西瓜叶片为试材,采用RT-PCR获得该病毒(CGMMV-LNYK)基因组全长(登录号MG745849),以pBluescriptⅡSK(-)为载体,分别...黄瓜绿斑驳花叶病毒Cucumber green mottle mosaic virus(CGMMV)是葫芦科作物的重要检疫性病毒。本研究以田间自然感病的西瓜叶片为试材,采用RT-PCR获得该病毒(CGMMV-LNYK)基因组全长(登录号MG745849),以pBluescriptⅡSK(-)为载体,分别克隆其所编码的4段基因,并采用人工接种验证它们的体外侵染活性。结果表明:该病毒与韩国分离物KW(登录号AF417242)亲缘关系最近,相似度达99.8%,并成功克隆了CGMMV-LNYK编码的4段基因,分别记为RNA1～RNA4。经人工接种,发现RNA1和RNA4能侵染葫芦,表现明显花叶症状,与该病毒接种葫芦症状一致,且接种RNA1后的症状较RNA4明显,4种RNA混合接种症状最为明显,经RT-PCR检测,发病植株可扩增到与接种的RNA大小相等的片段。展开更多
Type 2 diabetes mellitus(T2DM)is a multifactorial disease caused by both genetic and environmental factors.Although many genes have been reported to be involved in T2DM,much is still unknown about other genes that are...Type 2 diabetes mellitus(T2DM)is a multifactorial disease caused by both genetic and environmental factors.Although many genes have been reported to be involved in T2DM,much is still unknown about other genes that are involved in the disease and its progression.Therefore,the exploration of new factors plays a pivotal role in the development of new methods and strategies to prevent this chronic disease.展开更多
本文使用级磁变换(magnitude magnetic transforms,MMTs)对吉林省某矿区的磁异常进行了处理.MMTs包括总量异常(total magnitude anomaly,TMA)、TMA梯度的模、TMA Laplacian结果、TMA和TMALaplacian结果的乘积的平方根、TMA平方的Laplac...本文使用级磁变换(magnitude magnetic transforms,MMTs)对吉林省某矿区的磁异常进行了处理.MMTs包括总量异常(total magnitude anomaly,TMA)、TMA梯度的模、TMA Laplacian结果、TMA和TMALaplacian结果的乘积的平方根、TMA平方的Laplacian结果再开方取二分之一.MMTs得到的异常更接近此次研究矿区磁异常源的真实位置,实际解释更加容易.和传统的磁异常化极(reduction to the pole,RTP),磁源重力异常(pseudo-gravityanomaly)、解析延拓(analytical continuation)相比,MMTs只需要计算一阶水平导数,在低磁纬度地区的稳定性更强,并且不受地磁场矢量方向的影响.展开更多
文摘黄瓜绿斑驳花叶病毒Cucumber green mottle mosaic virus(CGMMV)是葫芦科作物的重要检疫性病毒。本研究以田间自然感病的西瓜叶片为试材,采用RT-PCR获得该病毒(CGMMV-LNYK)基因组全长(登录号MG745849),以pBluescriptⅡSK(-)为载体,分别克隆其所编码的4段基因,并采用人工接种验证它们的体外侵染活性。结果表明:该病毒与韩国分离物KW(登录号AF417242)亲缘关系最近,相似度达99.8%,并成功克隆了CGMMV-LNYK编码的4段基因,分别记为RNA1～RNA4。经人工接种,发现RNA1和RNA4能侵染葫芦,表现明显花叶症状,与该病毒接种葫芦症状一致,且接种RNA1后的症状较RNA4明显,4种RNA混合接种症状最为明显,经RT-PCR检测,发病植株可扩增到与接种的RNA大小相等的片段。
基金supported by the National Natural Science Foundation of China[Grants 81300702 to ZLL,81501199to WC]the Natural Scienceof Chongqing CSTC[cstc2018jcyj AXO210 to ZLL and cstc2017jcyj AX0016 to WC]。
文摘Type 2 diabetes mellitus(T2DM)is a multifactorial disease caused by both genetic and environmental factors.Although many genes have been reported to be involved in T2DM,much is still unknown about other genes that are involved in the disease and its progression.Therefore,the exploration of new factors plays a pivotal role in the development of new methods and strategies to prevent this chronic disease.
文摘本文使用级磁变换(magnitude magnetic transforms,MMTs)对吉林省某矿区的磁异常进行了处理.MMTs包括总量异常(total magnitude anomaly,TMA)、TMA梯度的模、TMA Laplacian结果、TMA和TMALaplacian结果的乘积的平方根、TMA平方的Laplacian结果再开方取二分之一.MMTs得到的异常更接近此次研究矿区磁异常源的真实位置,实际解释更加容易.和传统的磁异常化极(reduction to the pole,RTP),磁源重力异常(pseudo-gravityanomaly)、解析延拓(analytical continuation)相比,MMTs只需要计算一阶水平导数,在低磁纬度地区的稳定性更强,并且不受地磁场矢量方向的影响.
