The aging characteristics of the XLPE insulation of 110 kV power cables in the initial stage of operation are studied in this paper.The XLPE insulation from cables with different running time was tested by using elect...The aging characteristics of the XLPE insulation of 110 kV power cables in the initial stage of operation are studied in this paper.The XLPE insulation from cables with different running time was tested by using electrical and physicochemical methods,from which the degradation state of XLPE under multiple stresses during operation was derived.The broadband dielectric spectrum test taken from 10–1 to 10^(6) Hz showed that the dielectric constant and dielectric loss of the XLPE insulation first decrease and then increase with the increase of cable running time,which show obvious variation until 10 years of operation.The AC breakdown strengths of the XLPE insulation of operated cables with the service year no longer than 10 years were higher than that of new cables.The mechanical properties of the XLPE insulation showed no dependence with the cable running time.The crystallinity and perfection of the crystallization area were found to be enhanced during operation and the polar functional groups inside the XLPE insulation were found to be determined not only by the cross-linking by-products but also by the oxidation process during cable operation.The experimental results in this paper are important for improving the operation reliability and reducing the maintenance costs of the cables.展开更多
There are few studies on the membrane protein Ankfyl. We have found Ankfyl is specifically expressed in neural stem/precursor cells during early development in mice （murine）. To further explore Ankfyl function in ne...There are few studies on the membrane protein Ankfyl. We have found Ankfyl is specifically expressed in neural stem/precursor cells during early development in mice （murine）. To further explore Ankfyl function in neural development, we developed a gene knockout mouse with a mixed Balb/C and C57/BL6 genetic background. Using immunofluorescence and in situ hybridization, neural defects were absent in mixed genetic Ankfyl null mice during development and in adults up to 2 months old. However, Ankfyl gene knockout mice with a pure genetic background were found to be lethal in the C57/BL6 inbred mice embryos, even after seven generations of backcrossing. Polymerase chain reaction confirmed homozygotes were unattainable as early as embryonic day 11.5. We conclude that Ankfyl protein is dispensable in neural stem/precursor ceils, but could be critical for early embryonic murine development, depending on the genetic background.展开更多
Rimantadine(RIM),as a commonly used antiviral drug,is often illegally used in the aquaculture industry due to its remarkable therapeutic effect and low price,resulting in the accumulation of RIM in the environment and...Rimantadine(RIM),as a commonly used antiviral drug,is often illegally used in the aquaculture industry due to its remarkable therapeutic effect and low price,resulting in the accumulation of RIM in the environment and eventually threatening human health.Therefore,the realization of specific detection and monitoring of RIM has become an important topic in food safety and other fields.In this work,we screened an aptamer RIM-17 with high specificity and affinity for RIM based on Capture-systematic evolution of ligands by exponential enrichment(Capture-SELEX)technology.The binding mechanism and key sites of RIM and RIM-17 was studied by molecular docking technology.According to the molecular binding site,the optimal aptamer RIM-172 with a length of 50-mer was obtained by truncation optimization strategy,and its affinity Kd value for RIM was 46.84±11.33 nM.Based on the optimized aptamer,a surface-enhanced Raman spectroscopy(SERS)aptasensor with Fe_(3)O_(4)@UIO-66@AuNS as the Raman report substrate was established for the detection of RIM.Under the optimal preparation conditions,the linear range of the SERS-based aptasensor to detect RIM was 0.5~25 ng/mL and the limit of detection(LOD)was 0.27 ng/mL.In the test of added standard recovery,the recovery rate was 96.00%~101.20%.Moreover,the aptasensor exhibited accepted stability and specificity.The discovery of RIM aptamer provides an effective way for the specific capture and detection of RIM,and helps to monitor the illegal abuse of RIM.展开更多
The design of advanced dual-mode aptasensors has emerged as a promising strategy for the reliable detection of contaminants,with nanomaterial-mediated signal output playing a pivotal role.Herein,we developed a novel m...The design of advanced dual-mode aptasensors has emerged as a promising strategy for the reliable detection of contaminants,with nanomaterial-mediated signal output playing a pivotal role.Herein,we developed a novel multifunctional nanocomposite material(FePOs@CeO_(2))-mediated colorimetric/electrochemical dual-mode aptasensor for the sensitive and specific detection of amoxicillin(AMO),a widely used semi-synthetic peni-cillin.Therein,FePOs@CeO_(2)relied on the catalytic ability of FePOs and the excellent electrochemical perfor-mance of cerium’s multivalent states(Ce^(3+)/Ce^(4+))to promote effective electron transfer at the electrode interface,thereby exhibiting excellent peroxidase(POD)like activity and conductive properties.Furthermore,an aptamer AMO-1 with specific binding affinity for AMO was obtained and its truncated derivative(AMO-1b)with enhanced binding affinity was obtained through molecular docking simulation and secondary structure analysis.The specific binding of this high-affinity aptamer to AMO caused FePOs@CeO_(2)to detach from the electrode surface,thereby triggering a change in the electrochemical signal.The collected FePOs@CeO_(2)solution induced a color change,thereby enabling the colorimetric detection function.The sensing platform demonstrated high sensitivity,specificity,and accuracy in the detection of AMO,with detection limits of 0.828μg/mL(colorimetric)and 7.24×10^(-5)μg/mL(electrochemical).In addition,the sensor exhibited good practicality in the detection of AMO in milk,with recovery rates of 97.49%-98.25%(colorimetric)and 92.24%-93.01%(electrochemical),respectively.The dual detection results could verify each other,which improved the accuracy and reliability of the results and provided a strategy for the design of a novel biosensing platform for veterinary drug residue detection.展开更多
Colorectal cancer(CRC)is one of the most common malignant tumors worldwide,occurring in the colon or rectum portion.Quinoa is a dicotyledonous plant,and the quinoa bran is the outer seed coat of quinoa.Func-tional mol...Colorectal cancer(CRC)is one of the most common malignant tumors worldwide,occurring in the colon or rectum portion.Quinoa is a dicotyledonous plant,and the quinoa bran is the outer seed coat of quinoa.Func-tional molecules in plants play an important role in the treatment and alleviation of chronic diseases.In this study,quinoa bran was used as the raw material.The active components with an anti-colorectal cancer effect were extracted by acetone and separated with an AB-8 macroporous resin column,which was further identified as terpenoids by liquid-mass spectrometry(LMS),named Quinoa bran terpenoids(QBT).The yield of quinoa bran terpenoids was 0.77%.Moreover,the results showed that QBT significantly inhibited the proliferation of colo-rectal cancer cells DLD-1 and HCT-8 with a concentration gradient dependent manner.Respectively,the IC50 values of DLD-1 and HCT-8 cells were 0.42±0.02 and 0.54±0.05 mg/mL.Further studies showed that QBT upregulated apoptosis-related proteins such as activated-caspase-3,8,and 9.In addition,QBT decreased mito-chondrial membrane potential,promoted the expression of the pro-apoptotic protein Bax,and inhibited the antiapoptotic protein Bcl-2.The experiments showed that QBT significantly repressed the growth of tumors in nude mice.In conclusion,these results indicated that QBT largely inhibits the development of colorectal cancer to large extent through upregulation of caspase-3,8,and 9 and reduction of mitochondrial membrane potential,and has great potential for anti-tumor progression.展开更多
文摘The aging characteristics of the XLPE insulation of 110 kV power cables in the initial stage of operation are studied in this paper.The XLPE insulation from cables with different running time was tested by using electrical and physicochemical methods,from which the degradation state of XLPE under multiple stresses during operation was derived.The broadband dielectric spectrum test taken from 10–1 to 10^(6) Hz showed that the dielectric constant and dielectric loss of the XLPE insulation first decrease and then increase with the increase of cable running time,which show obvious variation until 10 years of operation.The AC breakdown strengths of the XLPE insulation of operated cables with the service year no longer than 10 years were higher than that of new cables.The mechanical properties of the XLPE insulation showed no dependence with the cable running time.The crystallinity and perfection of the crystallization area were found to be enhanced during operation and the polar functional groups inside the XLPE insulation were found to be determined not only by the cross-linking by-products but also by the oxidation process during cable operation.The experimental results in this paper are important for improving the operation reliability and reducing the maintenance costs of the cables.
基金Dr.Hui Fu was supported by the National Natural Science Foundation of China,No.81371338by Open Research Fund Program of Hubei-MOST KLOS & KLOBMEDr.Zu-neng Lu was supported by grants from Health and Family Planning Commission of Hubei Province scientific research project,No.WJ2015MA007
文摘There are few studies on the membrane protein Ankfyl. We have found Ankfyl is specifically expressed in neural stem/precursor cells during early development in mice （murine）. To further explore Ankfyl function in neural development, we developed a gene knockout mouse with a mixed Balb/C and C57/BL6 genetic background. Using immunofluorescence and in situ hybridization, neural defects were absent in mixed genetic Ankfyl null mice during development and in adults up to 2 months old. However, Ankfyl gene knockout mice with a pure genetic background were found to be lethal in the C57/BL6 inbred mice embryos, even after seven generations of backcrossing. Polymerase chain reaction confirmed homozygotes were unattainable as early as embryonic day 11.5. We conclude that Ankfyl protein is dispensable in neural stem/precursor ceils, but could be critical for early embryonic murine development, depending on the genetic background.
基金supported by the National Key Research and Development Program of China(2022YFF110110306)National Natural Science Foundation of China(32272449)+1 种基金the Fundamental Research Funds for the Central Universities(JUSRP622025)Collaborative Appendix A.Supplementary data。
文摘Rimantadine(RIM),as a commonly used antiviral drug,is often illegally used in the aquaculture industry due to its remarkable therapeutic effect and low price,resulting in the accumulation of RIM in the environment and eventually threatening human health.Therefore,the realization of specific detection and monitoring of RIM has become an important topic in food safety and other fields.In this work,we screened an aptamer RIM-17 with high specificity and affinity for RIM based on Capture-systematic evolution of ligands by exponential enrichment(Capture-SELEX)technology.The binding mechanism and key sites of RIM and RIM-17 was studied by molecular docking technology.According to the molecular binding site,the optimal aptamer RIM-172 with a length of 50-mer was obtained by truncation optimization strategy,and its affinity Kd value for RIM was 46.84±11.33 nM.Based on the optimized aptamer,a surface-enhanced Raman spectroscopy(SERS)aptasensor with Fe_(3)O_(4)@UIO-66@AuNS as the Raman report substrate was established for the detection of RIM.Under the optimal preparation conditions,the linear range of the SERS-based aptasensor to detect RIM was 0.5~25 ng/mL and the limit of detection(LOD)was 0.27 ng/mL.In the test of added standard recovery,the recovery rate was 96.00%~101.20%.Moreover,the aptasensor exhibited accepted stability and specificity.The discovery of RIM aptamer provides an effective way for the specific capture and detection of RIM,and helps to monitor the illegal abuse of RIM.
基金supported by the National Natural Science Foundation of China(32272449)the Basic Research Program of Jiangsu(BK20240100)+1 种基金the Fundamental Research Funds for the Central Universities(JUSRP622025)Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province.
文摘The design of advanced dual-mode aptasensors has emerged as a promising strategy for the reliable detection of contaminants,with nanomaterial-mediated signal output playing a pivotal role.Herein,we developed a novel multifunctional nanocomposite material(FePOs@CeO_(2))-mediated colorimetric/electrochemical dual-mode aptasensor for the sensitive and specific detection of amoxicillin(AMO),a widely used semi-synthetic peni-cillin.Therein,FePOs@CeO_(2)relied on the catalytic ability of FePOs and the excellent electrochemical perfor-mance of cerium’s multivalent states(Ce^(3+)/Ce^(4+))to promote effective electron transfer at the electrode interface,thereby exhibiting excellent peroxidase(POD)like activity and conductive properties.Furthermore,an aptamer AMO-1 with specific binding affinity for AMO was obtained and its truncated derivative(AMO-1b)with enhanced binding affinity was obtained through molecular docking simulation and secondary structure analysis.The specific binding of this high-affinity aptamer to AMO caused FePOs@CeO_(2)to detach from the electrode surface,thereby triggering a change in the electrochemical signal.The collected FePOs@CeO_(2)solution induced a color change,thereby enabling the colorimetric detection function.The sensing platform demonstrated high sensitivity,specificity,and accuracy in the detection of AMO,with detection limits of 0.828μg/mL(colorimetric)and 7.24×10^(-5)μg/mL(electrochemical).In addition,the sensor exhibited good practicality in the detection of AMO in milk,with recovery rates of 97.49%-98.25%(colorimetric)and 92.24%-93.01%(electrochemical),respectively.The dual detection results could verify each other,which improved the accuracy and reliability of the results and provided a strategy for the design of a novel biosensing platform for veterinary drug residue detection.
基金supported by the National Natural Science Founda-tion of China(No.32072220).
文摘Colorectal cancer(CRC)is one of the most common malignant tumors worldwide,occurring in the colon or rectum portion.Quinoa is a dicotyledonous plant,and the quinoa bran is the outer seed coat of quinoa.Func-tional molecules in plants play an important role in the treatment and alleviation of chronic diseases.In this study,quinoa bran was used as the raw material.The active components with an anti-colorectal cancer effect were extracted by acetone and separated with an AB-8 macroporous resin column,which was further identified as terpenoids by liquid-mass spectrometry(LMS),named Quinoa bran terpenoids(QBT).The yield of quinoa bran terpenoids was 0.77%.Moreover,the results showed that QBT significantly inhibited the proliferation of colo-rectal cancer cells DLD-1 and HCT-8 with a concentration gradient dependent manner.Respectively,the IC50 values of DLD-1 and HCT-8 cells were 0.42±0.02 and 0.54±0.05 mg/mL.Further studies showed that QBT upregulated apoptosis-related proteins such as activated-caspase-3,8,and 9.In addition,QBT decreased mito-chondrial membrane potential,promoted the expression of the pro-apoptotic protein Bax,and inhibited the antiapoptotic protein Bcl-2.The experiments showed that QBT significantly repressed the growth of tumors in nude mice.In conclusion,these results indicated that QBT largely inhibits the development of colorectal cancer to large extent through upregulation of caspase-3,8,and 9 and reduction of mitochondrial membrane potential,and has great potential for anti-tumor progression.
