The aging characteristics of the XLPE insulation of 110 kV power cables in the initial stage of operation are studied in this paper.The XLPE insulation from cables with different running time was tested by using elect...The aging characteristics of the XLPE insulation of 110 kV power cables in the initial stage of operation are studied in this paper.The XLPE insulation from cables with different running time was tested by using electrical and physicochemical methods,from which the degradation state of XLPE under multiple stresses during operation was derived.The broadband dielectric spectrum test taken from 10–1 to 10^(6) Hz showed that the dielectric constant and dielectric loss of the XLPE insulation first decrease and then increase with the increase of cable running time,which show obvious variation until 10 years of operation.The AC breakdown strengths of the XLPE insulation of operated cables with the service year no longer than 10 years were higher than that of new cables.The mechanical properties of the XLPE insulation showed no dependence with the cable running time.The crystallinity and perfection of the crystallization area were found to be enhanced during operation and the polar functional groups inside the XLPE insulation were found to be determined not only by the cross-linking by-products but also by the oxidation process during cable operation.The experimental results in this paper are important for improving the operation reliability and reducing the maintenance costs of the cables.展开更多
There are few studies on the membrane protein Ankfyl. We have found Ankfyl is specifically expressed in neural stem/precursor cells during early development in mice （murine）. To further explore Ankfyl function in ne...There are few studies on the membrane protein Ankfyl. We have found Ankfyl is specifically expressed in neural stem/precursor cells during early development in mice （murine）. To further explore Ankfyl function in neural development, we developed a gene knockout mouse with a mixed Balb/C and C57/BL6 genetic background. Using immunofluorescence and in situ hybridization, neural defects were absent in mixed genetic Ankfyl null mice during development and in adults up to 2 months old. However, Ankfyl gene knockout mice with a pure genetic background were found to be lethal in the C57/BL6 inbred mice embryos, even after seven generations of backcrossing. Polymerase chain reaction confirmed homozygotes were unattainable as early as embryonic day 11.5. We conclude that Ankfyl protein is dispensable in neural stem/precursor ceils, but could be critical for early embryonic murine development, depending on the genetic background.展开更多
Colorectal cancer(CRC)is one of the most common malignant tumors worldwide,occurring in the colon or rectum portion.Quinoa is a dicotyledonous plant,and the quinoa bran is the outer seed coat of quinoa.Func-tional mol...Colorectal cancer(CRC)is one of the most common malignant tumors worldwide,occurring in the colon or rectum portion.Quinoa is a dicotyledonous plant,and the quinoa bran is the outer seed coat of quinoa.Func-tional molecules in plants play an important role in the treatment and alleviation of chronic diseases.In this study,quinoa bran was used as the raw material.The active components with an anti-colorectal cancer effect were extracted by acetone and separated with an AB-8 macroporous resin column,which was further identified as terpenoids by liquid-mass spectrometry(LMS),named Quinoa bran terpenoids(QBT).The yield of quinoa bran terpenoids was 0.77%.Moreover,the results showed that QBT significantly inhibited the proliferation of colo-rectal cancer cells DLD-1 and HCT-8 with a concentration gradient dependent manner.Respectively,the IC50 values of DLD-1 and HCT-8 cells were 0.42±0.02 and 0.54±0.05 mg/mL.Further studies showed that QBT upregulated apoptosis-related proteins such as activated-caspase-3,8,and 9.In addition,QBT decreased mito-chondrial membrane potential,promoted the expression of the pro-apoptotic protein Bax,and inhibited the antiapoptotic protein Bcl-2.The experiments showed that QBT significantly repressed the growth of tumors in nude mice.In conclusion,these results indicated that QBT largely inhibits the development of colorectal cancer to large extent through upregulation of caspase-3,8,and 9 and reduction of mitochondrial membrane potential,and has great potential for anti-tumor progression.展开更多
文摘The aging characteristics of the XLPE insulation of 110 kV power cables in the initial stage of operation are studied in this paper.The XLPE insulation from cables with different running time was tested by using electrical and physicochemical methods,from which the degradation state of XLPE under multiple stresses during operation was derived.The broadband dielectric spectrum test taken from 10–1 to 10^(6) Hz showed that the dielectric constant and dielectric loss of the XLPE insulation first decrease and then increase with the increase of cable running time,which show obvious variation until 10 years of operation.The AC breakdown strengths of the XLPE insulation of operated cables with the service year no longer than 10 years were higher than that of new cables.The mechanical properties of the XLPE insulation showed no dependence with the cable running time.The crystallinity and perfection of the crystallization area were found to be enhanced during operation and the polar functional groups inside the XLPE insulation were found to be determined not only by the cross-linking by-products but also by the oxidation process during cable operation.The experimental results in this paper are important for improving the operation reliability and reducing the maintenance costs of the cables.
基金Dr.Hui Fu was supported by the National Natural Science Foundation of China,No.81371338by Open Research Fund Program of Hubei-MOST KLOS & KLOBMEDr.Zu-neng Lu was supported by grants from Health and Family Planning Commission of Hubei Province scientific research project,No.WJ2015MA007
文摘There are few studies on the membrane protein Ankfyl. We have found Ankfyl is specifically expressed in neural stem/precursor cells during early development in mice （murine）. To further explore Ankfyl function in neural development, we developed a gene knockout mouse with a mixed Balb/C and C57/BL6 genetic background. Using immunofluorescence and in situ hybridization, neural defects were absent in mixed genetic Ankfyl null mice during development and in adults up to 2 months old. However, Ankfyl gene knockout mice with a pure genetic background were found to be lethal in the C57/BL6 inbred mice embryos, even after seven generations of backcrossing. Polymerase chain reaction confirmed homozygotes were unattainable as early as embryonic day 11.5. We conclude that Ankfyl protein is dispensable in neural stem/precursor ceils, but could be critical for early embryonic murine development, depending on the genetic background.
基金supported by the National Natural Science Founda-tion of China(No.32072220).
文摘Colorectal cancer(CRC)is one of the most common malignant tumors worldwide,occurring in the colon or rectum portion.Quinoa is a dicotyledonous plant,and the quinoa bran is the outer seed coat of quinoa.Func-tional molecules in plants play an important role in the treatment and alleviation of chronic diseases.In this study,quinoa bran was used as the raw material.The active components with an anti-colorectal cancer effect were extracted by acetone and separated with an AB-8 macroporous resin column,which was further identified as terpenoids by liquid-mass spectrometry(LMS),named Quinoa bran terpenoids(QBT).The yield of quinoa bran terpenoids was 0.77%.Moreover,the results showed that QBT significantly inhibited the proliferation of colo-rectal cancer cells DLD-1 and HCT-8 with a concentration gradient dependent manner.Respectively,the IC50 values of DLD-1 and HCT-8 cells were 0.42±0.02 and 0.54±0.05 mg/mL.Further studies showed that QBT upregulated apoptosis-related proteins such as activated-caspase-3,8,and 9.In addition,QBT decreased mito-chondrial membrane potential,promoted the expression of the pro-apoptotic protein Bax,and inhibited the antiapoptotic protein Bcl-2.The experiments showed that QBT significantly repressed the growth of tumors in nude mice.In conclusion,these results indicated that QBT largely inhibits the development of colorectal cancer to large extent through upregulation of caspase-3,8,and 9 and reduction of mitochondrial membrane potential,and has great potential for anti-tumor progression.
