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茶多酚对大菱鲆生长、抗氧化能力及脂肪代谢相关基因表达的影响 被引量:10
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作者 李宜聪 廖凯 +4 位作者 姬仁磊 方炜 徐丹 麦康森 艾庆辉 《水产学报》 CAS CSCD 北大核心 2019年第11期2405-2412,共8页
为了研究饲料中添加茶多酚对大菱鲆生长、抗氧化能力及脂肪代谢相关基因表达的影响,以初始体质量为(13.51+0.31)g的大菱鲆幼鱼为实验对象,设计4组添加不同梯度茶多酚(0%、0.01%、0.02%和0.05%,干重添加量分别为0、100、200和500mg/kg)... 为了研究饲料中添加茶多酚对大菱鲆生长、抗氧化能力及脂肪代谢相关基因表达的影响,以初始体质量为(13.51+0.31)g的大菱鲆幼鱼为实验对象,设计4组添加不同梯度茶多酚(0%、0.01%、0.02%和0.05%,干重添加量分别为0、100、200和500mg/kg)的等氮等脂实验饲料,进行为期70d的摄食生长实验。结果显示:①与对照组相比,饲料中添加0.01%~0.02%茶多酚显著提高了大菱鲆幼鱼增重率(WGR);饲料效率(FE)随饲料中茶多酚添加水平升高而升高,但各组间差异不显著;随饲料中茶多酚添加水平升高,大菱鲆肝体比(HSI)呈降低趋势,且显著低于对照组;②鱼体组成分析表明,投喂添加茶多酚饲料组大菱鲆鱼体和肝脏粗脂肪含量呈下降趋势,且在茶多酚添加水平为0.02%~0.05%时达到最低值,显著低于对照组;③与对照组相比,投喂添加茶多酚饲料组大菱鲆血清总抗氧化能力(T-AOC)及谷胱甘肽过氧化物酶(GSH-Px)活性显著升高,且各添加组间差异不显著;超氧化物歧化酶(SOD)活性随茶多酚的添加水平升高呈先升高后降低趋势,且在添加水平为0.02%时显著高于对照组;血清丙二醛(MDA)含量随茶多酚添加水平升高而降低,且在添加水平为0.02%~0.05%时显著低于对照组;④大菱鲆肝脏固醇调节元件结合蛋白1(SREBP-1)表达量随着饲料中茶多酚添加水平升高而降低,且在添加水平为0.02%~0.05%时达到最低值,显著低于对照组;脂肪酸合成酶(FAS)表达量随茶多酚添加水平的升高呈先降低后升高趋势,且在添加水平为0.02%时显著低于对照组;过氧化物酶体增殖物激活受体α(PPARα)表达量变化趋势与FAS相反,且在添加水平为0.02%时达到最高值。肉毒碱棕榈酰转移酶1(CPT1)表达量随饲料中茶多酚添加水平升高而升高,显著高于对照组,且各添加组间差异不显著。研究表明,高脂饲料中添加茶多酚能促进大菱鲆生长、降低肝脏脂肪过度沉积并提高血清抗氧化能力,高脂饲料中添加0.02%茶多酚是大菱鲆幼鱼生长的最适添加量。 展开更多
关键词 大菱鲆 茶多酚 生长 体组成 抗氧化能力 脂肪代谢
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A Study on the Meat and Bone Meal and Poultry By-product Meal as Protein Substitutes of Fish Meal in Practical Diets for Litopenaeus vannamei Juveniles 被引量:5
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作者 ZHUWei maikangsen +2 位作者 ZHANGBaigang WANGFuzhen YUYu 《Journal of Ocean University of China》 SCIE CAS 2004年第2期157-160,共4页
A study was conducted to evaluate the effects of meat and bone meal (MBM) and poultry by-product meal (PBM) as the replacement of fish meal in the diets on the growth performance, survival and apparent digestibility c... A study was conducted to evaluate the effects of meat and bone meal (MBM) and poultry by-product meal (PBM) as the replacement of fish meal in the diets on the growth performance, survival and apparent digestibility coefficients (ADC) of Litopenaeus vannamei. The basal diets were formulated with 22% fish meal and other ingredients which provided about 40% protein and 9% lipid in the diet. The experimental diets included MBM or PBM to replace 0, 20%, 40%, 60% and 80% of total fish meal respectively. All diets were iso-nitrogenous and isocaloric in gross terms. The results showed that there were no significant differences (P>0.05) in growth performance and ADC among the treatments fed with the diets in which 0-60% fish meal had been replaced with MBM, while the percent weight gain (WG, %), body length gain (BLG, %) and ADC significantly decreased when the MBM was up to 80% of the fish meal. There were no significant differences (P>0.05) in growth performance and ADC among all the treatments fed with the diets in which 0-80% fish meal had been replaced with PBM. 展开更多
关键词 Litopenaeus vannamei fish meal substitute growth performance apparent digestibility coefficient (ADC) meat and bone meal (MBM) poultry by-product meal (PBM)
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Advances in the Study on Nutrient Requirements of Grouper (Epinephelus sp.): a Review 被引量:1
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作者 LUOZhi LIUYongjian +1 位作者 maikangsen TIANLixia 《Journal of Ocean University of China》 SCIE CAS 2005年第2期93-98,共6页
The paper reviews the recent advances in studying grouper nutrition requirement for the development of cost-effective and environmentally friendly artificial diets. It consists of seven parts: protein and amino acid, ... The paper reviews the recent advances in studying grouper nutrition requirement for the development of cost-effective and environmentally friendly artificial diets. It consists of seven parts: protein and amino acid, lipid and essential fatty acid, carbohydrate, vitamin, mineral, alternative protein source, broodstock and larval nutrition. The review provides some basic information for further investigation of nutrient requirements of groupers. 展开更多
关键词 EPINEPHELUS GROUPER nutrient requirement fish nutrition
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Effect of E. coli coli on Anti-disease Activities of Scailops: Argopecten irradians
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作者 胡晓珂 JiangXiaolu +1 位作者 LiuShiliang maikangsen 《High Technology Letters》 EI CAS 2003年第1期11-16,共6页
The effect of acute E. coli challenge on the anti-disease activity of scallops Argopecten irra-dians is examined. The treatments of scallop from which hemolymph samples were taken for study included (1) control scallo... The effect of acute E. coli challenge on the anti-disease activity of scallops Argopecten irra-dians is examined. The treatments of scallop from which hemolymph samples were taken for study included (1) control scallops, (2) sham-injected scallops, (3) PSW-injected scallops and (4) E. coli-injected scallops. From the beginning, the anti-disease activities of scallops are deter -mined at 12 hr and 24 hr.The concentrations of circulating hemocytes, the total serum protein concentrations and the activities of alkaline phosphatase, acid phosphatase and superoxide dismutase in the scallops Argopecten irradians are determined.Injection with E. coli results in a significant elevation in the concentration of circulating hemocytes and in the alkaline phosphatase activity and a significant decline in the total serum protein concentration and in the superoxide dismutase activity at 24 hr postchallenge. It shows that metabolism of bay scallop is expedited to adopt the challenge. 展开更多
关键词 Argopecten irradians E. coli HEMOCYTES HEMOLYMPH alkaline phosphatase acid phosphatase superoxide diamutase MOLLUSK
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