Objective To explore the effect of hepatocyte growth factor （HGF） and fibroblast growth factor （FGF） on preimplantation embryo development of mouse.Methods Mated mice were killed by cervical dislocation to collect...Objective To explore the effect of hepatocyte growth factor （HGF） and fibroblast growth factor （FGF） on preimplantation embryo development of mouse.Methods Mated mice were killed by cervical dislocation to collect two pronucleous （2 PN） zygotes from oviduct of pregnant 1 d NMRI mice and were cultured to the hatching blastocyst stage and the number of embryo in different stages was recorded under an invert microscope. The cleavage rates of formed 2 PN zygotes were compared with blastocyst and hatching blastocyst stage in drops of T6 medium with or without HGF or FGF （0 ng/ml, 10 ng/ml, 20 ng/ml, 50 ng/ml, 1 000 ng/ml）. Results HGF and FGF enriched embryo culture media promotea the aevetopment from 2 PN stage embryos to blastocyst. Adding 20 ng/ml of FGF or HGF to the culture medium significantly increased the percentage of 2PN embryos that developed into blastocysts （P〈0.05）, but culture of embryos in drops of T6 medium with HGF （20 ng/ml） had no improvement in in-vitro hatching.Conclusion Exogenous HGF and FGF at low concentrations promote in-vitro mouse blastocyst formation.展开更多
Premature ovarian failure(POF)is defined as the loss of normal ovarian function before the age of 40 and is characterized by increased gonadotropin levels and decreased estradiol levels and ovarian reserve,often leadi...Premature ovarian failure(POF)is defined as the loss of normal ovarian function before the age of 40 and is characterized by increased gonadotropin levels and decreased estradiol levels and ovarian reserve,often leading to infertility.The incomplete understanding of the pathogenesis of POF is a major impediment to the development of effective treatments for this disease,so the use of animal models is a promising option for investigating and identifying the molecular mechanisms involved in POF patients and developing therapeutic agents.As mice and rats are the most commonly used models in animal research,this review article considers studies that used murine POF models.In this review based on the most recent studies,first,we introduce 10 different methods for inducing murine POF models,then we demonstrate the advantages and disadvantages of each one,and finally,we suggest the most practical method for inducing a POF model in these animals.This may help researchers find the method of creating a POF model that is most appropriate for their type of study and suits the purpose of their research.展开更多
文摘Objective To explore the effect of hepatocyte growth factor （HGF） and fibroblast growth factor （FGF） on preimplantation embryo development of mouse.Methods Mated mice were killed by cervical dislocation to collect two pronucleous （2 PN） zygotes from oviduct of pregnant 1 d NMRI mice and were cultured to the hatching blastocyst stage and the number of embryo in different stages was recorded under an invert microscope. The cleavage rates of formed 2 PN zygotes were compared with blastocyst and hatching blastocyst stage in drops of T6 medium with or without HGF or FGF （0 ng/ml, 10 ng/ml, 20 ng/ml, 50 ng/ml, 1 000 ng/ml）. Results HGF and FGF enriched embryo culture media promotea the aevetopment from 2 PN stage embryos to blastocyst. Adding 20 ng/ml of FGF or HGF to the culture medium significantly increased the percentage of 2PN embryos that developed into blastocysts （P〈0.05）, but culture of embryos in drops of T6 medium with HGF （20 ng/ml） had no improvement in in-vitro hatching.Conclusion Exogenous HGF and FGF at low concentrations promote in-vitro mouse blastocyst formation.
文摘Premature ovarian failure(POF)is defined as the loss of normal ovarian function before the age of 40 and is characterized by increased gonadotropin levels and decreased estradiol levels and ovarian reserve,often leading to infertility.The incomplete understanding of the pathogenesis of POF is a major impediment to the development of effective treatments for this disease,so the use of animal models is a promising option for investigating and identifying the molecular mechanisms involved in POF patients and developing therapeutic agents.As mice and rats are the most commonly used models in animal research,this review article considers studies that used murine POF models.In this review based on the most recent studies,first,we introduce 10 different methods for inducing murine POF models,then we demonstrate the advantages and disadvantages of each one,and finally,we suggest the most practical method for inducing a POF model in these animals.This may help researchers find the method of creating a POF model that is most appropriate for their type of study and suits the purpose of their research.
