The objectives of this study were to evaluate the chemical composition,antioxidant and microbicide properties,and cytotoxic effects of essential oils acquired from Laurel(Laurus nobilis),Clove(Syzygium aromaticum),and...The objectives of this study were to evaluate the chemical composition,antioxidant and microbicide properties,and cytotoxic effects of essential oils acquired from Laurel(Laurus nobilis),Clove(Syzygium aromaticum),and Cinnamon(Cinnamomum verum)by hydrodistillation.GC-MS analysis identified 1,8-cineole(48.50%),eugenol(49.49%),and cinnamaldehyde(65.34%)as the major constituents of laurel,clove,and cinnamon essential oils,respectively.Antioxidant capacity,assessed by ABTS^(+)and DPPH assays,revealed that cinnamon essential oil exhibited the highest inhibition percentage.Antimicrobial testing showed cinnamon oil was most effective against tested pathogens,with an average inhibition zone of 28 mm.On the other hand,cytotoxicity was evaluated using the MTT assay on L-929 fibroblast cells,and no harmful effects were observed for clove and cinnamon oils.The protective effect of the oils has been tested against total mesophilic aerobic bacteria(TMAB)and yeast-mold(YM)count in a preservative-free tomato paste model food environment with a 25-day storage period.At the end of 25 days,the control group had 3×10^(2) cfu/g excess colony formation,but the samples treated with 1.5%concentration had 1.5×10^(1) cfu/g mean count.Furthermore,a hand sanitizer formulation containing these oils suppressed microorganisms by 85%,whereas the addition of isopropyl alcohol(25%)increased the activity to 98%.These findings demonstrate that clove and cinnamon essential oils possess strong inhibitory effects against microbial growth and oxidation,supporting their potential as natural alternatives to synthetic preservatives and disinfectants.展开更多
The authors regret that the above-mentioned article lacked descrip-tive text in the legend of Fig.2.The correct and complete legend for Fig.2 should read as follows:Fig.2.Graphics depicting the particle size distribut...The authors regret that the above-mentioned article lacked descrip-tive text in the legend of Fig.2.The correct and complete legend for Fig.2 should read as follows:Fig.2.Graphics depicting the particle size distribution of essential oils utilized in the present study.展开更多
Lactiplantibacillus plantarum is a significant probiotic where it could be found in ubiquitous niches.In this study,a new Lb.plantarum strain DY46 was isolated from a traditional lactic-acid-fermented beverage called ...Lactiplantibacillus plantarum is a significant probiotic where it could be found in ubiquitous niches.In this study,a new Lb.plantarum strain DY46 was isolated from a traditional lactic-acid-fermented beverage called shalgam.The whole genome of the DY46 was sequenced and obtained sequences were assembled into a 3.32 Mb draft genome using PATRIC(3.6.8.).The DY46 genome consists of a single circular chromosome of 3,332,827 bp that is predicted to carry 3219 genes,including 61 tRNA genes,2 rRNA operons.The genome has a GC content of 44.3%includes 98 predicted pseudogenes,25 complete or partial transposases and 3 intact prophages.The genes encoding enzymes related in the intact EMP(Embden-Meyerhof-Parnas)and PK(phosphoketolase)pathways were predicted using BlastKOALA which is an indicator of having facultative heterofermentative pathways.DY46 genome also predicted to carry genes of Pln E,Pln F and Pln K showing the antimicrobial potential of this bacterium which can be linked to in vitro antagonism tests that DY46 can inhibit S.enterica sv.Typhimurium ATCC14028,K.pneumonie ATCC13883,and P.vulgaris ATCC8427.Moreover,it is determined that all resistome found in its genome were intrinsically originated and the strain was found to be tolerant to acid and bile concentrations by mimicking human gastrointestinal conditions.In conclusion,L.plantarum DY46 is a promising bacterium that appears to have certain probiotic properties,confirmed by“in vitro”and“in silico”analyses,and is a potential dietary supplement candidate that may provide functional benefits to the host.展开更多
基金supported by Erciyes University Scientific Project Unit under grant number of FYL-2021-10764 as Msc thesis。
文摘The objectives of this study were to evaluate the chemical composition,antioxidant and microbicide properties,and cytotoxic effects of essential oils acquired from Laurel(Laurus nobilis),Clove(Syzygium aromaticum),and Cinnamon(Cinnamomum verum)by hydrodistillation.GC-MS analysis identified 1,8-cineole(48.50%),eugenol(49.49%),and cinnamaldehyde(65.34%)as the major constituents of laurel,clove,and cinnamon essential oils,respectively.Antioxidant capacity,assessed by ABTS^(+)and DPPH assays,revealed that cinnamon essential oil exhibited the highest inhibition percentage.Antimicrobial testing showed cinnamon oil was most effective against tested pathogens,with an average inhibition zone of 28 mm.On the other hand,cytotoxicity was evaluated using the MTT assay on L-929 fibroblast cells,and no harmful effects were observed for clove and cinnamon oils.The protective effect of the oils has been tested against total mesophilic aerobic bacteria(TMAB)and yeast-mold(YM)count in a preservative-free tomato paste model food environment with a 25-day storage period.At the end of 25 days,the control group had 3×10^(2) cfu/g excess colony formation,but the samples treated with 1.5%concentration had 1.5×10^(1) cfu/g mean count.Furthermore,a hand sanitizer formulation containing these oils suppressed microorganisms by 85%,whereas the addition of isopropyl alcohol(25%)increased the activity to 98%.These findings demonstrate that clove and cinnamon essential oils possess strong inhibitory effects against microbial growth and oxidation,supporting their potential as natural alternatives to synthetic preservatives and disinfectants.
文摘The authors regret that the above-mentioned article lacked descrip-tive text in the legend of Fig.2.The correct and complete legend for Fig.2 should read as follows:Fig.2.Graphics depicting the particle size distribution of essential oils utilized in the present study.
基金This study has been financially supported by Erciyes University Scientific Research Projects Coordination Unit under grant number FKB-2020-10551.
文摘Lactiplantibacillus plantarum is a significant probiotic where it could be found in ubiquitous niches.In this study,a new Lb.plantarum strain DY46 was isolated from a traditional lactic-acid-fermented beverage called shalgam.The whole genome of the DY46 was sequenced and obtained sequences were assembled into a 3.32 Mb draft genome using PATRIC(3.6.8.).The DY46 genome consists of a single circular chromosome of 3,332,827 bp that is predicted to carry 3219 genes,including 61 tRNA genes,2 rRNA operons.The genome has a GC content of 44.3%includes 98 predicted pseudogenes,25 complete or partial transposases and 3 intact prophages.The genes encoding enzymes related in the intact EMP(Embden-Meyerhof-Parnas)and PK(phosphoketolase)pathways were predicted using BlastKOALA which is an indicator of having facultative heterofermentative pathways.DY46 genome also predicted to carry genes of Pln E,Pln F and Pln K showing the antimicrobial potential of this bacterium which can be linked to in vitro antagonism tests that DY46 can inhibit S.enterica sv.Typhimurium ATCC14028,K.pneumonie ATCC13883,and P.vulgaris ATCC8427.Moreover,it is determined that all resistome found in its genome were intrinsically originated and the strain was found to be tolerant to acid and bile concentrations by mimicking human gastrointestinal conditions.In conclusion,L.plantarum DY46 is a promising bacterium that appears to have certain probiotic properties,confirmed by“in vitro”and“in silico”analyses,and is a potential dietary supplement candidate that may provide functional benefits to the host.
