We investigated the effect of black sea bream extracts on changes in pro-inflammatory and anti-inflammatory cytokines induced by lipopolysaccharide(LPS)in murine splenocytes.The levels of pro-inflammatory cytokines[in...We investigated the effect of black sea bream extracts on changes in pro-inflammatory and anti-inflammatory cytokines induced by lipopolysaccharide(LPS)in murine splenocytes.The levels of pro-inflammatory cytokines[interleukin(IL)-6,tumor necrosis factor-α(TNF-α),IL-12/IL-23(p40)and IL-17A]and anti-inflammatory cytokines[IL-4,IL-10 and interferon-γ(IFN-γ)]were assessed.Incubation of murine splenocytes with acetone+methylene chloride(A+M)and methanol(MeOH)extracts significantly decreased LPS-induced IL-6,IL-12/IL-23(p40)and IL-17A productions after 6 h incubation(p<0.05).The A+M and MeOH extracts significantly increased LPS-induced IL-4 and IFN-γproductions at 48 and 72 h incubation(p<0.05).Treatment with A+M extract resulted in significantly higher IL-10 production in splenocytes after 72 h(p<0.05).Conclusively,black sea bream extracts were shown to be efficient in falling several pro-inflammatory cytokines while rising anti-inflammatory cytokines.Thus our results suggest that black sea bream extracts selectivity modulate immune events.展开更多
基金Basic Science Research Program through the National Research Foundation of Korea(NRF)funded by the Ministry of Science,ICT and Future Planning(NRF-2017R1A2B4005915)。
文摘We investigated the effect of black sea bream extracts on changes in pro-inflammatory and anti-inflammatory cytokines induced by lipopolysaccharide(LPS)in murine splenocytes.The levels of pro-inflammatory cytokines[interleukin(IL)-6,tumor necrosis factor-α(TNF-α),IL-12/IL-23(p40)and IL-17A]and anti-inflammatory cytokines[IL-4,IL-10 and interferon-γ(IFN-γ)]were assessed.Incubation of murine splenocytes with acetone+methylene chloride(A+M)and methanol(MeOH)extracts significantly decreased LPS-induced IL-6,IL-12/IL-23(p40)and IL-17A productions after 6 h incubation(p<0.05).The A+M and MeOH extracts significantly increased LPS-induced IL-4 and IFN-γproductions at 48 and 72 h incubation(p<0.05).Treatment with A+M extract resulted in significantly higher IL-10 production in splenocytes after 72 h(p<0.05).Conclusively,black sea bream extracts were shown to be efficient in falling several pro-inflammatory cytokines while rising anti-inflammatory cytokines.Thus our results suggest that black sea bream extracts selectivity modulate immune events.
