目的 探究腭裂(Cleft Palate, CP)患儿伴发分泌性中耳炎(otitis media with effusion, OME)的发病特点。方法 纳入拟于首都医科大学附属北京口腔医院整形创伤科行腭裂修复术的腭裂患儿300例(600耳),年龄6月龄~6岁,正常对照51例(102耳)...目的 探究腭裂(Cleft Palate, CP)患儿伴发分泌性中耳炎(otitis media with effusion, OME)的发病特点。方法 纳入拟于首都医科大学附属北京口腔医院整形创伤科行腭裂修复术的腭裂患儿300例(600耳),年龄6月龄~6岁,正常对照51例(102耳)。所有研究对象在术前均完善声导抗和听性脑干反应检查(ABR),记录并分析腭裂患儿听力检测结果。结果腭裂患儿OME的发病率为83%。伴OME的腭裂患儿主要表现为轻度听力损失。患有分泌性中耳炎与健听腭裂患儿ABRⅤ波阈值平均值及Ⅰ、Ⅲ、Ⅴ波潜伏期显著差异(P<0.05),患有OME的腭裂患儿ABRⅤ波阈值平均值增加,Ⅰ、Ⅲ、Ⅴ波潜伏期延长。Ⅰ~Ⅲ、Ⅰ~Ⅴ波间期无差异(P>0.05)。是否伴发唇裂与OME发病率以及听力损失程度无显著相关性(P>0.05)。不同类型腭裂患儿OME发病率、听力损失程度无差异(P>0.05)。结论 腭裂患儿OME的发病率明显高于非腭裂儿童(1.6%~30.7%),总体听力损失程度较重,腭裂类型及伴发唇裂与OME的发病率及听力损失程度无相关性,提示腭裂患儿治疗时应重点关注患儿的听力状况,若患儿出现听力下降情况,应及时进行干预。展开更多
The Sustainable Development Goals(SDGs)are crucial in tackling the sustainability challenges and emerging issues faced by humanity,with government attention being a significant factor in promoting their successful ach...The Sustainable Development Goals(SDGs)are crucial in tackling the sustainability challenges and emerging issues faced by humanity,with government attention being a significant factor in promoting their successful achievement.However,there is limited quantitative research systematically examining the impacts of government attention on SDGs progress.This study employs text analysis and a panel regression model to analyze the impacts of government attention intensity,text similarity,and tone on the achievement of SDGs,utilizing data extracted from China’s Government Work Reports spanning the decade from 2010 to 2020.The findings reveal that the Chinese government attention to the SDGs has generally increased over time.The heightened focus has notably bolstered the achievement of the SDGs,with the most significant impact observed post-2015.Government attention intensity was identified as the most impactful factor.Moreover,government attention intensity,text similarity,and tone have positively influenced the coupling coordination relationship between 17 SDGs,as measured by the coupling coordination degree,leading to a more harmonious and balanced achievement of socioeconomic and environmental goals in China.Financial investment served as a moderating factor,enhancing the positive impacts of attention intensity,text similarity and tone on the promotion of SDGs attainment.The effects of government attention on SDGs progress were notably positive in the eastern region,exhibiting greater significance in areas with stronger governance capacity compared to those with weaker governance capacity.This study provides insightful information for enhancing the modernization and efficiency of China’s national governance system,promoting SDGs at local and global scales,and fostering sustainable transformation.展开更多
目的阐释古代经典名方“保元汤”的药效物质基础并探讨其分子水平的作用机制,发掘保元汤更多潜在的临床应用优势。方法采用UPLC-QTOF-MS/MS技术,选用Waters ACQUITY UPLC HSS T3(100 mm×2.1 mm,1.7μm)色谱柱,以0.1%甲酸水(A)-乙腈...目的阐释古代经典名方“保元汤”的药效物质基础并探讨其分子水平的作用机制,发掘保元汤更多潜在的临床应用优势。方法采用UPLC-QTOF-MS/MS技术,选用Waters ACQUITY UPLC HSS T3(100 mm×2.1 mm,1.7μm)色谱柱,以0.1%甲酸水(A)-乙腈(B)为流动相系统进行梯度洗脱,高分辨飞行时间质谱电喷雾电离源(ESI),正、负离子扫描模式,检测鉴定保元汤的化学成分组成,并分析保元汤大鼠多次多天ig给药后含药血浆中的原型化学成分及代谢产物,进一步运用网络药理学方法,利用TCMSP数据库和药物信息数据库等分析保元汤入血原型成分的靶向作用,并揭示其治疗潜能。结果根据精确相对分子质量数据和多级质谱碎片离子,结合对照品、数据库以及文献报道,共鉴定出保元汤中133个化学成分,并且确定大鼠ig保元汤后,35个成分能够经口吸收入血达到血浆稳态的药物浓度。保元汤入血原型成分“成分-靶点”网络图、核心靶基因基因本体(gene ontology,GO)功能富集分析、京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)通路富集分析、疾病预测分析表明,保元汤可通过人参皂苷Rb1、丹皮酚、刺芒柄花素、刺甘草查耳酮、大豆异黄酮、甘草查耳酮A、6-姜辣素、水杨酸等8个关键成分作用于丝裂原活化蛋白激酶1(mitogen-activated protein kinase 1,MAPK1)、肿瘤蛋白p53(tumor protein p53,TP53)、信号传导及转录激活蛋白(signal transducer and activator of transcription)等核心靶点,通过糖基化终末产物(advanced glycation end products,AGE)-晚期糖基化终末产物受体(receptor for advanced glycation end-products,RAGE)、表皮生长因子受体(epidermal growth factor receptor,EGFR)、非受体酪氨酸激酶(janus kinase,JAK)-信号转导子和转录激活子(signal transducer and activator of transcription,STAT)等信号通路等途径发挥药效作用,提示保元汤除治疗慢性心力衰竭外,还对阿尔茨海默症、再灌注损伤、绝经后骨质疏松症等具有潜在治疗优势。保元汤35个入血原型成分中含有多个同类成分的衍生物,且其中既有单一成分对接多个靶点、又有多个类似物对接同一靶点的情况,由此佐证了“中药(复方)多成分多靶点协同作用”和“中药多成分单靶点叠加作用”的理论。结论采用高分辨质谱分析技术和网络药理学及其数据库,可以为全面阐释中药(复方)药效物质基础及探讨其分子水平的作用机制提供可行的科学方法。明确了古代经典名方保元汤的入血化学成分,鉴定了其原型成分的代谢产物,并从分子水平阐释了基于这些入血原型成分的作用靶点,并揭示其潜在治疗优势,为扩大该方剂临床应用提供参考,发掘更多的临床治疗潜能。展开更多
中药黄芪活性成分毛蕊异黄酮(CA),是从黄芪中提取出的功能性植物雌激素异黄酮,具有高效、毒副作用小、多靶点等特性,包含抗氧化、抗辐射、抗菌、保护心脑血管、增强免疫等多重药理作用。此外,多项研究还发现CA具有显著的抗肿瘤生物作用...中药黄芪活性成分毛蕊异黄酮(CA),是从黄芪中提取出的功能性植物雌激素异黄酮,具有高效、毒副作用小、多靶点等特性,包含抗氧化、抗辐射、抗菌、保护心脑血管、增强免疫等多重药理作用。此外,多项研究还发现CA具有显著的抗肿瘤生物作用,有望成为治疗恶性肿瘤的潜在药物成分。研究表明,CA可通过多种作用机制发挥抗肿瘤作用,例如诱导肿瘤细胞凋亡、抑制肿瘤细胞增殖及迁移及侵袭等,且CA在乳腺癌、结直肠癌、肺癌、宫颈癌、卵巢癌、鼻咽癌等多种常见恶性肿瘤中均有研究表明具有抗肿瘤功效,其抗肿瘤活性的作用机制主要体现于可通过调节B细胞淋巴瘤-2(Bcl-2)家族基因,微小RNA(miRNA),雌激素受体β(ERβ)触发肿瘤细胞凋亡;其抗增殖活性主要体现在对周期蛋白(Cyclin)家族,WD repeatcontaining protein 7(WDR7-7)蛋白,尤文肉瘤相关转录物1(EWSAT1)等的调节作用;通过阻断上皮间质转化(EMT)进程,血管内皮生长因子(VEGF),基质金属蛋白酶(MMP)抑制肿瘤细胞的转移和侵袭。此外,还可通过调节自噬标志物-1(Beclin-1)诱导肿瘤细胞自噬发挥抗肿瘤作用,且CA还可增强对化疗药物的敏感性以加强治疗效果。虽CA抗肿瘤研究基数较大,作用广泛,效果佳,但缺乏对CA抗肿瘤作用机制的系统性综述,为此,本研究基于CA抗肿瘤作用机制进行文献综述,以期为科研研究者和临床工作者提供借鉴及信息参考。展开更多
文摘目的 探究腭裂(Cleft Palate, CP)患儿伴发分泌性中耳炎(otitis media with effusion, OME)的发病特点。方法 纳入拟于首都医科大学附属北京口腔医院整形创伤科行腭裂修复术的腭裂患儿300例(600耳),年龄6月龄~6岁,正常对照51例(102耳)。所有研究对象在术前均完善声导抗和听性脑干反应检查(ABR),记录并分析腭裂患儿听力检测结果。结果腭裂患儿OME的发病率为83%。伴OME的腭裂患儿主要表现为轻度听力损失。患有分泌性中耳炎与健听腭裂患儿ABRⅤ波阈值平均值及Ⅰ、Ⅲ、Ⅴ波潜伏期显著差异(P<0.05),患有OME的腭裂患儿ABRⅤ波阈值平均值增加,Ⅰ、Ⅲ、Ⅴ波潜伏期延长。Ⅰ~Ⅲ、Ⅰ~Ⅴ波间期无差异(P>0.05)。是否伴发唇裂与OME发病率以及听力损失程度无显著相关性(P>0.05)。不同类型腭裂患儿OME发病率、听力损失程度无差异(P>0.05)。结论 腭裂患儿OME的发病率明显高于非腭裂儿童(1.6%~30.7%),总体听力损失程度较重,腭裂类型及伴发唇裂与OME的发病率及听力损失程度无相关性,提示腭裂患儿治疗时应重点关注患儿的听力状况,若患儿出现听力下降情况,应及时进行干预。
基金supported by Guizhou Province Major Science and Technology Achievement Transformation Project(QKHCG[2024]ZD016)the Excellent Young Scientists Fund from the National Natural Science Foundation of China(Grant No.42422105)+1 种基金Guizhou Province Natural Science Research Project(Qian Jiao Ji[2023]No.033)Provincial Science and Technology Program of Guizhou Province(Grant No.20201Y288).
文摘The Sustainable Development Goals(SDGs)are crucial in tackling the sustainability challenges and emerging issues faced by humanity,with government attention being a significant factor in promoting their successful achievement.However,there is limited quantitative research systematically examining the impacts of government attention on SDGs progress.This study employs text analysis and a panel regression model to analyze the impacts of government attention intensity,text similarity,and tone on the achievement of SDGs,utilizing data extracted from China’s Government Work Reports spanning the decade from 2010 to 2020.The findings reveal that the Chinese government attention to the SDGs has generally increased over time.The heightened focus has notably bolstered the achievement of the SDGs,with the most significant impact observed post-2015.Government attention intensity was identified as the most impactful factor.Moreover,government attention intensity,text similarity,and tone have positively influenced the coupling coordination relationship between 17 SDGs,as measured by the coupling coordination degree,leading to a more harmonious and balanced achievement of socioeconomic and environmental goals in China.Financial investment served as a moderating factor,enhancing the positive impacts of attention intensity,text similarity and tone on the promotion of SDGs attainment.The effects of government attention on SDGs progress were notably positive in the eastern region,exhibiting greater significance in areas with stronger governance capacity compared to those with weaker governance capacity.This study provides insightful information for enhancing the modernization and efficiency of China’s national governance system,promoting SDGs at local and global scales,and fostering sustainable transformation.
文摘目的阐释古代经典名方“保元汤”的药效物质基础并探讨其分子水平的作用机制,发掘保元汤更多潜在的临床应用优势。方法采用UPLC-QTOF-MS/MS技术,选用Waters ACQUITY UPLC HSS T3(100 mm×2.1 mm,1.7μm)色谱柱,以0.1%甲酸水(A)-乙腈(B)为流动相系统进行梯度洗脱,高分辨飞行时间质谱电喷雾电离源(ESI),正、负离子扫描模式,检测鉴定保元汤的化学成分组成,并分析保元汤大鼠多次多天ig给药后含药血浆中的原型化学成分及代谢产物,进一步运用网络药理学方法,利用TCMSP数据库和药物信息数据库等分析保元汤入血原型成分的靶向作用,并揭示其治疗潜能。结果根据精确相对分子质量数据和多级质谱碎片离子,结合对照品、数据库以及文献报道,共鉴定出保元汤中133个化学成分,并且确定大鼠ig保元汤后,35个成分能够经口吸收入血达到血浆稳态的药物浓度。保元汤入血原型成分“成分-靶点”网络图、核心靶基因基因本体(gene ontology,GO)功能富集分析、京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)通路富集分析、疾病预测分析表明,保元汤可通过人参皂苷Rb1、丹皮酚、刺芒柄花素、刺甘草查耳酮、大豆异黄酮、甘草查耳酮A、6-姜辣素、水杨酸等8个关键成分作用于丝裂原活化蛋白激酶1(mitogen-activated protein kinase 1,MAPK1)、肿瘤蛋白p53(tumor protein p53,TP53)、信号传导及转录激活蛋白(signal transducer and activator of transcription)等核心靶点,通过糖基化终末产物(advanced glycation end products,AGE)-晚期糖基化终末产物受体(receptor for advanced glycation end-products,RAGE)、表皮生长因子受体(epidermal growth factor receptor,EGFR)、非受体酪氨酸激酶(janus kinase,JAK)-信号转导子和转录激活子(signal transducer and activator of transcription,STAT)等信号通路等途径发挥药效作用,提示保元汤除治疗慢性心力衰竭外,还对阿尔茨海默症、再灌注损伤、绝经后骨质疏松症等具有潜在治疗优势。保元汤35个入血原型成分中含有多个同类成分的衍生物,且其中既有单一成分对接多个靶点、又有多个类似物对接同一靶点的情况,由此佐证了“中药(复方)多成分多靶点协同作用”和“中药多成分单靶点叠加作用”的理论。结论采用高分辨质谱分析技术和网络药理学及其数据库,可以为全面阐释中药(复方)药效物质基础及探讨其分子水平的作用机制提供可行的科学方法。明确了古代经典名方保元汤的入血化学成分,鉴定了其原型成分的代谢产物,并从分子水平阐释了基于这些入血原型成分的作用靶点,并揭示其潜在治疗优势,为扩大该方剂临床应用提供参考,发掘更多的临床治疗潜能。
文摘中药黄芪活性成分毛蕊异黄酮(CA),是从黄芪中提取出的功能性植物雌激素异黄酮,具有高效、毒副作用小、多靶点等特性,包含抗氧化、抗辐射、抗菌、保护心脑血管、增强免疫等多重药理作用。此外,多项研究还发现CA具有显著的抗肿瘤生物作用,有望成为治疗恶性肿瘤的潜在药物成分。研究表明,CA可通过多种作用机制发挥抗肿瘤作用,例如诱导肿瘤细胞凋亡、抑制肿瘤细胞增殖及迁移及侵袭等,且CA在乳腺癌、结直肠癌、肺癌、宫颈癌、卵巢癌、鼻咽癌等多种常见恶性肿瘤中均有研究表明具有抗肿瘤功效,其抗肿瘤活性的作用机制主要体现于可通过调节B细胞淋巴瘤-2(Bcl-2)家族基因,微小RNA(miRNA),雌激素受体β(ERβ)触发肿瘤细胞凋亡;其抗增殖活性主要体现在对周期蛋白(Cyclin)家族,WD repeatcontaining protein 7(WDR7-7)蛋白,尤文肉瘤相关转录物1(EWSAT1)等的调节作用;通过阻断上皮间质转化(EMT)进程,血管内皮生长因子(VEGF),基质金属蛋白酶(MMP)抑制肿瘤细胞的转移和侵袭。此外,还可通过调节自噬标志物-1(Beclin-1)诱导肿瘤细胞自噬发挥抗肿瘤作用,且CA还可增强对化疗药物的敏感性以加强治疗效果。虽CA抗肿瘤研究基数较大,作用广泛,效果佳,但缺乏对CA抗肿瘤作用机制的系统性综述,为此,本研究基于CA抗肿瘤作用机制进行文献综述,以期为科研研究者和临床工作者提供借鉴及信息参考。
