1T-MoS_(2)nanosheets,with metallic conductivity and high capacity,hold great potential for lithium-ion capacitors(LICs),but suffer from sluggish reaction kinetics due to dense stacking.Herein,1T-MoS_(2)nanosheets with...1T-MoS_(2)nanosheets,with metallic conductivity and high capacity,hold great potential for lithium-ion capacitors(LICs),but suffer from sluggish reaction kinetics due to dense stacking.Herein,1T-MoS_(2)nanosheets with enlarged interlayer spacing,vertically bonded to reduced graphene oxide(rGO)(1T-MoS_(2)/rGO),were designed using a hydrothermal-assisted dispersion and intercalation strategy.The active nitrogen species derived from N,N-dimethylformamide(DMF)not only bridge the rGO and MoS_(2)through strong Mo-N-C bonds to promote the formation of dispersed MoS_(2)nanosheets,but also intercalate into the MoS_(2)structure,further enlarging the interlayer spacing.This unique structure synergistically enhances meso-and microscale mass transfer outside and inside of the few-layered nanosheets,significantly improving electrochemical reaction kinetics and reducing the kinetic mismatch between the anode and cathode.Consequently,the resulting 1T-MoS_(2)/rGO achieves a capacity of 500 mAh g^(-1)after 500 cycles at 5 A g^(-1)and a high rate performance of 587 mAh g^(-1)at a high rate of 10 A g^(-1).Moreover,the assembled 3D vertical 1T-MoS_(2)/rGO//AC LIC delivers a high energy density of 100.3 Wh kg^(-1)at a power density of1.0 kW kg^(-1),and long cycle stability with capacity retention as high as 91.02%after 5000 cycles at 2 A g^(-1).This work provides a generalizable strategy for engineering two-dimensional material-based electrodes,offering new insights into high-performance energy storage systems.展开更多
The activation of some oncogenes promote cancer cell proliferation and growth,facilitate cancer progression and metastasis by induce DNA replication stress,even genome instability.Activation of the cyclic GMP-AMP synt...The activation of some oncogenes promote cancer cell proliferation and growth,facilitate cancer progression and metastasis by induce DNA replication stress,even genome instability.Activation of the cyclic GMP-AMP synthase(cGAS)mediates classical DNA sensing,is involved in genome instability,and is linked to various tumor development or therapy.However,the function of cGAS in gastric cancer remains elusive.In this study,the TCGA database and retrospective immunohistochemical analyses revealed substantially high cGAS expression in gastric cancer tissues and cell lines.By employing cGAS high-expression gastric cancer cell lines,including AGS and MKN45,ectopic silencing of cGAS caused a significant reduction in the proliferation of the cells,tumor growth,and mass in xenograft mice.Mechanistically,database analysis predicted a possible involvement of cGAS in the DNA damage response(DDR),further data through cells revealed protein interactions of the cGAS and MRE11-RAD50-NBN(MRN)complex,which activated cell cycle checkpoints,even increased genome instability in gastric cancer cells,thereby contributing to gastric cancer progression and sensitivity to treatment with DNA damaging agents.Furthermore,the upregulation of cGAS significantly exacerbated the prognosis of gastric cancer patients while improving radiotherapeutic outcomes.Therefore,we concluded that cGAS is involved in gastric cancer progression by fueling genome instability,implying that intervening in the cGAS pathway could be a practicable therapeutic approach for gastric cancer.展开更多
The healing of diabetic wounds is primarily hindered by persistent inflammation and excessive oxidative stress,increasing the risks of amputation and sepsis.Strategies based on bioactive substances,including recombina...The healing of diabetic wounds is primarily hindered by persistent inflammation and excessive oxidative stress,increasing the risks of amputation and sepsis.Strategies based on bioactive substances,including recombinant growth factors and histatin proteins(Hsts),have been shown to promote skin-related cell migration,anti-inflammation,angiogenesis,and collagen deposition;however,their long-term stability remains a challenge.Herein,a platelet membrane-coated nanoparticle(PNP)system is proposed to achieve enhanced retention of aggregation-induced emissive(AIE)molecular-modified Hst1(Hst1-AIE@PNPs)for more efficient repair of diabetic wounds.The Hst1-AIE@PNPs can not only protect Hst1 from degradation in the wound microenvironment but also permit visual monitoring of the controlled release of Hst1 through enhanced fluorescence in the enriched site.Combined with the antioxidant and anti-inflammatory properties of Hst1,Hst1-AIE@PNPs can effectively adsorb inflammation-related factors and further promote re-epithelialization and collagen deposition,thus achieving high-quality wound repair.The results highlight the potential of highly stable aggregation-induced-emissionfunctionalized Hst1 coated with platelet vesicles as a therapeutic platform to promote diabetic wound-related tissue restoration processes.展开更多
Treponema pallidum(T.pallidum)causes syphilis,a sexually transmitted disease that leads to multi-organ complications and even death.The lack of technology for tracing live T.pallidum greatly impedes understanding of t...Treponema pallidum(T.pallidum)causes syphilis,a sexually transmitted disease that leads to multi-organ complications and even death.The lack of technology for tracing live T.pallidum greatly impedes understanding of the pathogenesis of T.pallidum.Herein,we firstly designed and developed an in situ high-resolution imaging strategy to trace live T.pallidum using catalyst-free bioorthogonal labeling with aggregation-induced emission luminogens(AIEgens).An activated alkyne-containing AIEgen,5-(4-(diphenylamino)phenylthiophene-2-ynylidene)ketone(named TPA-TA),was directly reacted with the proteins of T.pallidum through metal-free click chemistry,without affecting T.pallidum’s activity.Specially,TPA-TA enables high-resolution imaging of live T.pallidum with low background.Both whole phcagocytosis of T.pallidum by THP-1 cells and dissemination in lesions could be monitored in real time,which helped clarify the interaction between the immune clearance and escape of T.pallidum.In conclusion,this catalyst-free bioorthogonal labeling strategy enriches the toolkit for exploring and understanding syphilis-related pathologies.展开更多
基金the financial support from the National Natural Science Foundation of China(No.52225208 and 51802131)the Training Program for academic and technical leaders in major disciplines of Jiangxi Province-Young Talents(No.20212BCJ23021)the Natural Science Foundation of Jiangxi Province,China(No.20232BAB204020).
文摘1T-MoS_(2)nanosheets,with metallic conductivity and high capacity,hold great potential for lithium-ion capacitors(LICs),but suffer from sluggish reaction kinetics due to dense stacking.Herein,1T-MoS_(2)nanosheets with enlarged interlayer spacing,vertically bonded to reduced graphene oxide(rGO)(1T-MoS_(2)/rGO),were designed using a hydrothermal-assisted dispersion and intercalation strategy.The active nitrogen species derived from N,N-dimethylformamide(DMF)not only bridge the rGO and MoS_(2)through strong Mo-N-C bonds to promote the formation of dispersed MoS_(2)nanosheets,but also intercalate into the MoS_(2)structure,further enlarging the interlayer spacing.This unique structure synergistically enhances meso-and microscale mass transfer outside and inside of the few-layered nanosheets,significantly improving electrochemical reaction kinetics and reducing the kinetic mismatch between the anode and cathode.Consequently,the resulting 1T-MoS_(2)/rGO achieves a capacity of 500 mAh g^(-1)after 500 cycles at 5 A g^(-1)and a high rate performance of 587 mAh g^(-1)at a high rate of 10 A g^(-1).Moreover,the assembled 3D vertical 1T-MoS_(2)/rGO//AC LIC delivers a high energy density of 100.3 Wh kg^(-1)at a power density of1.0 kW kg^(-1),and long cycle stability with capacity retention as high as 91.02%after 5000 cycles at 2 A g^(-1).This work provides a generalizable strategy for engineering two-dimensional material-based electrodes,offering new insights into high-performance energy storage systems.
基金supported by Zhengzhou Major Collaborative Innovation Project(No.18XTZX12003)Key Projects of Discipline Construction in Zhengzhou University(No.XKZDJC202001)+2 种基金National Key Research and Development Program in China(No.2020YFC2006100)Excellent Foreign Scientist Studio of Henan Province in China(No.GZS2018001)Medical Service Capacity Improvement Project of Henan Province in China(Grant No.Yu Wei Medicine[2017]No.66).
文摘The activation of some oncogenes promote cancer cell proliferation and growth,facilitate cancer progression and metastasis by induce DNA replication stress,even genome instability.Activation of the cyclic GMP-AMP synthase(cGAS)mediates classical DNA sensing,is involved in genome instability,and is linked to various tumor development or therapy.However,the function of cGAS in gastric cancer remains elusive.In this study,the TCGA database and retrospective immunohistochemical analyses revealed substantially high cGAS expression in gastric cancer tissues and cell lines.By employing cGAS high-expression gastric cancer cell lines,including AGS and MKN45,ectopic silencing of cGAS caused a significant reduction in the proliferation of the cells,tumor growth,and mass in xenograft mice.Mechanistically,database analysis predicted a possible involvement of cGAS in the DNA damage response(DDR),further data through cells revealed protein interactions of the cGAS and MRE11-RAD50-NBN(MRN)complex,which activated cell cycle checkpoints,even increased genome instability in gastric cancer cells,thereby contributing to gastric cancer progression and sensitivity to treatment with DNA damaging agents.Furthermore,the upregulation of cGAS significantly exacerbated the prognosis of gastric cancer patients while improving radiotherapeutic outcomes.Therefore,we concluded that cGAS is involved in gastric cancer progression by fueling genome instability,implying that intervening in the cGAS pathway could be a practicable therapeutic approach for gastric cancer.
基金supported by the China Postdoctoral Science Foundation(grant number 2023M731548)the National Natural Science Foundation of China(grant numbers 82322042,82102444)+2 种基金the National Key Research and Development Program of China(grant number 2021YFC2302200)the Natural Science Fund of Guangdong Province for Distinguished Young Scholars(grant number 2022B1515020089)the Basic and Applied Basic Research Project of Guangzhou(grant number SL2023A04J01463).
文摘The healing of diabetic wounds is primarily hindered by persistent inflammation and excessive oxidative stress,increasing the risks of amputation and sepsis.Strategies based on bioactive substances,including recombinant growth factors and histatin proteins(Hsts),have been shown to promote skin-related cell migration,anti-inflammation,angiogenesis,and collagen deposition;however,their long-term stability remains a challenge.Herein,a platelet membrane-coated nanoparticle(PNP)system is proposed to achieve enhanced retention of aggregation-induced emissive(AIE)molecular-modified Hst1(Hst1-AIE@PNPs)for more efficient repair of diabetic wounds.The Hst1-AIE@PNPs can not only protect Hst1 from degradation in the wound microenvironment but also permit visual monitoring of the controlled release of Hst1 through enhanced fluorescence in the enriched site.Combined with the antioxidant and anti-inflammatory properties of Hst1,Hst1-AIE@PNPs can effectively adsorb inflammation-related factors and further promote re-epithelialization and collagen deposition,thus achieving high-quality wound repair.The results highlight the potential of highly stable aggregation-induced-emissionfunctionalized Hst1 coated with platelet vesicles as a therapeutic platform to promote diabetic wound-related tissue restoration processes.
基金supported by the National Key Research and Development Program of China(no.2024YFC231103)the National Natural Science Foundation of China(no.82102444,82322042,82272248)+3 种基金the Natural Science Fund of Guangdong Province for Distinguished Young Scholars(2022B1515020089)Guangdong Basic and Applied Basic Research Foundation(no.2023A1515140123)Basic and Applied Basic Research Project of Guangzhou(SL2023A04J01463)Open Competition Mechanism to Select the Best Candidates for Key Research Projects of Ningxia Medical University(XJKF230124).
文摘Treponema pallidum(T.pallidum)causes syphilis,a sexually transmitted disease that leads to multi-organ complications and even death.The lack of technology for tracing live T.pallidum greatly impedes understanding of the pathogenesis of T.pallidum.Herein,we firstly designed and developed an in situ high-resolution imaging strategy to trace live T.pallidum using catalyst-free bioorthogonal labeling with aggregation-induced emission luminogens(AIEgens).An activated alkyne-containing AIEgen,5-(4-(diphenylamino)phenylthiophene-2-ynylidene)ketone(named TPA-TA),was directly reacted with the proteins of T.pallidum through metal-free click chemistry,without affecting T.pallidum’s activity.Specially,TPA-TA enables high-resolution imaging of live T.pallidum with low background.Both whole phcagocytosis of T.pallidum by THP-1 cells and dissemination in lesions could be monitored in real time,which helped clarify the interaction between the immune clearance and escape of T.pallidum.In conclusion,this catalyst-free bioorthogonal labeling strategy enriches the toolkit for exploring and understanding syphilis-related pathologies.
