Cardiovascular disease is the leading cause of global mortality,with anticoagulant therapy being the main prevention and treatment strategy.Recombinant hirudin(r-hirudin)is a direct thrombin inhibitor that can potenti...Cardiovascular disease is the leading cause of global mortality,with anticoagulant therapy being the main prevention and treatment strategy.Recombinant hirudin(r-hirudin)is a direct thrombin inhibitor that can potentially prevent thrombosis via subcutaneous(SC)and intravenous(IV)administration,but there is a risk of haemorrhage via SC and IV.Thus,microneedle(MN)provides painless and sanitary alternatives to syringes and oral administration.However,the current technological process for the micro mould is complicated and expensive.The micro mould obtained via three-dimensional(3D)printing is expected to save time and cost,as well as provide a diverse range of MNs.Therefore,we explored a method for MNs array model production based on 3D printing and translate it to micro mould that can be used for fabrication of dissolving MNs patch.The results show that r-hirudin-loaded and hyaluronic acid(HA)-based MNs can achieve transdermal drug delivery and exhibit significant potential in the prevention of thromboembolic disease without bleeding in animal models.These results indicate that based on 3D printing technology,MNs combined with r-hirudin are expected to achieve diverse customizableMNs and thus realize personalized transdermal anticoagulant delivery for minimally invasive and long-term treatment of thrombotic disease.展开更多
A new skeleton bisabolane-type sesquiterpene curcuminoid,bisabocurcumin（1）,along with 5 known compounds,curcumin（2）, demethoxycurcumin（3）,bidemethoxycurcumin（4）,（1E,4E）-1,5-bis（4-hydroxy-3-methoxyphenyl）-p...A new skeleton bisabolane-type sesquiterpene curcuminoid,bisabocurcumin（1）,along with 5 known compounds,curcumin（2）, demethoxycurcumin（3）,bidemethoxycurcumin（4）,（1E,4E）-1,5-bis（4-hydroxy-3-methoxyphenyl）-penta-1,4-dien-3-one（5）,and （1E,4E）-1-（4-hydroxy-3-methoxyphenyl）-5-（4-hydroxy phenyl-）-penta-1,4-dien-3-one（6）were isolated from the rhizomes of Curcuma longa L.Their structures were determined on the basis of spectroscopic analysis.Bisabocurcumin（1） is firstly obtained from nature with a new skeleton combined by a bisabolane-type sesquiterpene and a 1,7-diphenylheptanoid through a C-C bond.展开更多
Static tensile and fatigue tests were performed on shear and tensile self-piercing riveted aluminium-steel structures to evaluate their mechanical and fatigue properties. The influences of the thickness and the streng...Static tensile and fatigue tests were performed on shear and tensile self-piercing riveted aluminium-steel structures to evaluate their mechanical and fatigue properties. The influences of the thickness and the strength of the high strength steel on mechanical and fatigue performances were investigated based on the tensile and F-N curves of the joints. The results show that mechanical and fatigue properties of the shear self-piercing riveted joints are much better than those of the tensile self-piercing riveted joints. Mechanical and fatigue performances of the two joints were significantly influenced by the thickness and strength of the steel sheet, and were markedly improved when the thickness of steel sheet increased. The steel strength showed significantly different effects on shear and tensile riveted structures, i. e. , when the steel strength increased, the strength of the shear structure greatly increased while the tensile structure just had a slight increase in the strength. Fatigue failure generally occurred in the sheet materials and the fatigue crack location changed with increasing the sheet thickness and the sheet strength.展开更多
Human immunodeficiency virus type 1 （HIV-1） Vpr induces cell death in mammalian and fssion yeast cells, suggesting that Vpr may affect a conserved cellular process. It is unclear, however, whether Vpr-induced yeast ...Human immunodeficiency virus type 1 （HIV-1） Vpr induces cell death in mammalian and fssion yeast cells, suggesting that Vpr may affect a conserved cellular process. It is unclear, however, whether Vpr-induced yeast cell death mimics Vpr-mediated apoptosis in mammalian cells. We have recently identified a number of Vpr suppressors that not only suppress Vpr-induced cell death in fission yeast, but also block Vpr-induced apoptosis in mammalian cells. These findings suggest that Vpr-induced cell death in yeast may resemble some of the apoptotic processes of mammalian cells. The goal of this study was to develop and validate a fission yeast model system for future studies of apoptosis. Similar to Vpr-induced apoptosis in mammalian cells, we show here that Vpr in fission yeast promotes phosphatidylserine externalization and induces hyperpolarization of mitochondria, leading to changes of mitochondrial membrane potential. Moreover, Vpr triggers production of reactive oxygen species （ROS）, indicating that the apoptotic-like cell death might be mediated by ROS. Interestingly, Vpr induces unique morphologic changes in mitochondria that may provide a simple marker for measuring the apoptotic-like process in fission yeast. To verify this possibility, we tested two Vpr suppressors （EF2 and Hspl6） that suppress Vpr-induced apoptosis in mammalian cells in addition to a newly identified Vpr suppressor （Skpl）. All three proteins abolished cell death mediated by Vpr and restored normal mitochondrial morphology in the yeast cells. In conclusion, Vpr-induced cell death in fission yeast resembles the mammalian apoptotic process. Fission yeast may thus potentially be used as a simple model organism for the future study of the apoptotic-like process induced by Vpr and other proapoptotic agents.展开更多
Aluminum(Al)toxicity poses a significant constraint on field crop yields in acid soils.Zinc finger protein36(ZFP36)is well-documented for its pivotal role in enhancing tolerance to both drought and oxidative stress in...Aluminum(Al)toxicity poses a significant constraint on field crop yields in acid soils.Zinc finger protein36(ZFP36)is well-documented for its pivotal role in enhancing tolerance to both drought and oxidative stress in rice.This study unveils a novel function of ZFP36 modulated by abscisic acid(ABA)-dependent mechanisms,specifically aimed at alleviating Al toxicity in rice.Under Al stress,the expression of ZFP36significantly increased through an ABA-dependent pathway.Knocking down ZFP36 heightened Al sensitivity,while overexpressing ZFP36 conferred increased resistance to Al stress.Additionally,our investigations revealed a physical interaction between ZFP36 and pyruvate dehydrogenase kinase 1 in rice(OsPDK1).Biochemical assays further elucidated that OsPDK1 phosphorylates ZFP36 at the amino acid site 73–161.Subsequent experiments demonstrated that ZFP36 positively regulates the expression of ascorbate peroxidases(OsAPX1)and OsALS1 by binding to specific elements in their upstream segments in rice.Through genetic and phenotypic analyses,we unveiled that OsPDK1 influences ABA-triggered antioxidant defense to alleviate Al toxicity by interacting with ZFP36.In summary,our study underscores that pyruvate dehydrogenase kinase 1(OsPDK1)phosphorylates ZFP36 to modulate the activities of antioxidant enzymes via an ABA-dependent pathway,influencing tolerance of rice to soil Al toxicity.展开更多
[Objectives] This study was conducted to investigate the effects of different treatments on rooting and germination of Paeonia rockii T. Hong et J.J.Li seeds. [Methods] Different stratification time and temperatures a...[Objectives] This study was conducted to investigate the effects of different treatments on rooting and germination of Paeonia rockii T. Hong et J.J.Li seeds. [Methods] Different stratification time and temperatures and different GA_3 concentrations and treatment time were set to screen the optimum treatment method for promoting seed rooting and germination. [Results] The rooting rate was the highest when the seeds were subjected to 30 d of stratification treatment in an incubator at 25℃, and treated with 300 mg/L GA_3 for 24 h. Soaking the seeds with 300 mg/L GA_3 for 24 h combined with 30 d of stratification treatment was most beneficial to germination, and the germination rate was over 90%. [Conclusions] This study provides technical guidance and basic information for the rooting and germination of P. rockii seeds.展开更多
[Objective] This study aimed to identify a maunanase-producing strain isolated from soil. [Method] With kanjac powder as the substrate, a man- nanase-producing dominant strain was iselated from the soil samples collec...[Objective] This study aimed to identify a maunanase-producing strain isolated from soil. [Method] With kanjac powder as the substrate, a man- nanase-producing dominant strain was iselated from the soil samples collected from Kunyu Mountain by using plate selection method. Sequence analysis of the 16SrDNA fragment of the strain was conducted, and the strain was identified as Bacillus subtilis. Fermentation conditions and enzymatic characteristics were studied preliminarily. [ Result] Experimental result showed that enzyme yield of this strain was different in different medium and in the same medium at different tempera- ture. Enzyme yield of this strain in LB medium was higher when incubated at 27 ℃ than at 30 ℃ ; however, incubation at 30 ℃ was more conducive to the enzyme production than incubation at 27 ℃ in SOC medium. The optimal reaction pH was 7.0 and the optimal reaction temperature was 55 ℃ for enzyme production of this strain. When the temperature was above 55 ℃, enzyme activity declined sharply with the raise of temperature. Under the optimum conditions, enzyme activity could achieve 95.3 U. [ Conclusion] This study provided reference for the industrial application of degradation products of mannan.展开更多
As a kind of ionic artificial muscle material,Ionic Polymer-Metal Composites(IPMCs)have the advantages of a low drive current,light weight,and significant flexibility.IPMCs are widely used in the fields of biomedicine...As a kind of ionic artificial muscle material,Ionic Polymer-Metal Composites(IPMCs)have the advantages of a low drive current,light weight,and significant flexibility.IPMCs are widely used in the fields of biomedicine,soft robots,etc.However,the displacement and blocking force of the traditional sheet-type Nafion-IPMC need to be improved,and it has the limitation of unidirectional actuation.In this paper,a new type of short side chain Aquivion material is used as the polymer in the IPMC.The cylindrical IPMC is prepared by extrusion technology to improve its actuation performance and realize multi-degree-of-freedom motion.In comparison to the traditional Nafion-IPMC,the ion exchange capacity,specific capacitance,and conductivity of Aquivion-IPMC are improved by 28%,27%,and 32%,respectively,and the displacement and blocking force are improved by 57%and 25%,respectively.The cylindrical actuators can be deflected in eight directions.This indicates that Aquivion,as a polymer membrane for IPMC,holds significant application potential.By designing a cylindrical IPMC electrode distribution,the multi-degree-of-freedom deflection of IPMC can be realized.展开更多
In order to effectively solve the problems of low accuracy,large amount of computation and complex logic of deep learning algorithms in behavior recognition,a kind of behavior recognition based on the fusion of 3 dime...In order to effectively solve the problems of low accuracy,large amount of computation and complex logic of deep learning algorithms in behavior recognition,a kind of behavior recognition based on the fusion of 3 dimensional batch normalization visual geometry group(3D-BN-VGG)and long short-term memory(LSTM)network is designed.In this network,3D convolutional layer is used to extract the spatial domain features and time domain features of video sequence at the same time,multiple small convolution kernels are stacked to replace large convolution kernels,thus the depth of neural network is deepened and the number of network parameters is reduced.In addition,the latest batch normalization algorithm is added to the 3-dimensional convolutional network to improve the training speed.Then the output of the full connection layer is sent to LSTM network as the feature vectors to extract the sequence information.This method,which directly uses the output of the whole base level without passing through the full connection layer,reduces the parameters of the whole fusion network to 15324485,nearly twice as much as those of 3D-BN-VGG.Finally,it reveals that the proposed network achieves 96.5%and 74.9%accuracy in the UCF-101 and HMDB-51 respectively,and the algorithm has a calculation speed of 1066 fps and an acceleration ratio of 1,which has a significant predominance in velocity.展开更多
OBJECTIVE:To take Baihui(GV20)and Fengchi(GB20)targeting inflammatory response to regulate migraine as an example to describe a new method for studying the mechanism of stimulating acupoints.METHODS:The target informa...OBJECTIVE:To take Baihui(GV20)and Fengchi(GB20)targeting inflammatory response to regulate migraine as an example to describe a new method for studying the mechanism of stimulating acupoints.METHODS:The target information of Baihui(GV20)and Fengchi(GB20)was retrieved,and after intersection with migraine,Kyoto Encyclopedia of Genes and Genomes(KEGG),Reactome,and Uni Prot Keywords were used for functional enrichment.After selecting the main pathway,rats were selected and nitroglycerin was used for modeling,and the behavioral scores,inflammatory factors,heme oxygenase 1(HMOX1),protein kinase B(AKT1),signal transducer and activator of transcription 3(STAT3),phosphorylated extracellular signal-regulated kinase 1/2(P-ERK1/ERK2)and other states of the rats in the acupuncture,twisting,and electroacupuncture groups were compared.RESULTS:A total of 135 Baihui(GV20)targets and 27 Fengchi(GB20)targets were collected.A total of 73 target information were obtained after the intersection of these targets in migraine.These 73 targets have three main pathways:hypoxia-inducible factor 1(HIF-1)signaling pathway,signaling by interleukins and inflammatory response.The main targets in the pathway were verified and found that interleukin-1 beta(IL-1β),interleukin-6(IL-6),tumor necrosis factor-alpha(TNF-α)and HMOX1,AKT1,STAT3,P-ERK1/ERK2 can be regulated by Baihui(GV20)and Fengchi(GB20).CONCLUSION:Baihui(GV20)and Fengchi(GB20)can regulate migraine by regulating inflammatory factors and HMOX1,AKT1,STAT3,P-ERK1/ERK2 and other changes in HIF-1 signaling pathway,Signaling by Interleukins and Inflammatory response pathways.Based on systems biology and network pharmacology,and with the model of"acupoint-target-disease",explore the research methods of systematic acupuncture and moxibustion.We believe this is a usable research direction for exploring the mechanism of acupuncture stimulation.展开更多
Surgical electrodes are frequently associated with disadvantages such as high surface adhesion and severe thermal damage to adjacent normal tissues,which threaten operation quality and patient safety.In this study,by ...Surgical electrodes are frequently associated with disadvantages such as high surface adhesion and severe thermal damage to adjacent normal tissues,which threaten operation quality and patient safety.In this study,by mimicking the micromorphology and bio-anti-adhesion of shark skin,we proposed a strategy that utilized nanoscale aluminium oxide(Al_(2)O_(3))films deposited on bioinspired shark skin(BSS)microstructures to design a composite surface(Al_(2)O_(3)@BSS)and integrated it into both flat sides of the surgical electrodes.Micro/nano-manufacturing of the Al_(2)O_(3)@BSS surface was sequentially accomplished using nanosecond laser texturing,atomic layer deposition,and low-temperature annealing,endowing it with excellent blood-repellent properties.Visualisation experiments revealed that the tensile stress gradient of the blood coagulum with increasing thickness under a thermal field prompted it to separate from the Al_(2)O_(3)@BSS surface,resulting in anti-adhesion.Furthermore,it was observed for the first time that Al_(2)O_(3) films could transiently excite discharge along a dielectric surface(DADS)to ablate tissues while suppressing Joule heat,thereby minimising thermal damage.A combination of ex vivo tissue and living mouse experiments demonstrated that the Al_(2)O_(3)@BSS electrodes exhibited optimal comprehensive performance in terms of anti-adhesion,damage minimisation,and drag reduction.In addition,the Al_(2)O_(3)@BSS electrodes possessed remarkable antibacterial efficacy against E.coli and S.aureus.The proposed strategy can meet the extreme application requirements of surgical electrodes to improve operation quality and offer valuable insights for future studies.展开更多
BACKGROUND Ganglioneuroma is a rare,well-differentiated,slow-growing benign tumor of the peripheral nerves,with surgical resection being the only curative treatment.Surgical resection of ganglioneuromas encasing major...BACKGROUND Ganglioneuroma is a rare,well-differentiated,slow-growing benign tumor of the peripheral nerves,with surgical resection being the only curative treatment.Surgical resection of ganglioneuromas encasing major blood vessels remains a substantial clinical challenge.Traditionally,these cases often require open abdominal surgery or combined organ resections,and in some instances,the tumors are considered unresectable.Currently,no reports have described the resection of such tumors via laparoscopy.CASE SUMMARY A 35-year-old woman was admitted to our hospital after the incidental discovery of a retroperitoneal space-occupying lesion.Imaging revealed a mass with the celiac axis and superior mesenteric artery passing through it.A neurogenic tumor was suspected,with ganglioneuroma being the most likely diagnosis.Following comprehensive preoperative preparation,the retroperitoneal tumor was resected using a three-dimensional laparoscopy combined with an organ suspension technique.The surgical approach involved incising the tumor along the vascular axis and conducting meticulous,vascular-preserving tumor excision.The operation lasted approximately 458 minutes,with an estimated blood loss of 50 mL.The patient was discharged on the 8th postoperative day.A transient liver injury occurred after surgery but improved rapidly.After 11 months of postoperative follow-up,no complications or tumor recurrence were observed.CONCLUSION This case illustrates the feasibility of minimally invasive laparoscopic resection for retroperitoneal ganglioneuromas encasing major blood vessels.展开更多
BACKGROUND Intraductal oncocytic papillary neoplasm(IOPN)of the pancreas is an extremely rare pancreatic tumor,with only sporadic cases reported in the literature.IOPN is difficult to diagnose and highly prone to misd...BACKGROUND Intraductal oncocytic papillary neoplasm(IOPN)of the pancreas is an extremely rare pancreatic tumor,with only sporadic cases reported in the literature.IOPN is difficult to diagnose and highly prone to misdiagnosis.IOPN carries a certain risk of progressing to invasive cancer.Surgical resection is the primary treatment for IOPN.According to the existing literature reports,the vast majority of patients with IOPN of the pancreas undergo open surgery,while only one case of laparoscopic surgery have been reported.CASE SUMMARY This report presents two cases of IOPN in elderly female patients,aged 60 and 61.Both patients were asymptomatic,and their pancreatic masses were discovered incidentally.Preoperative diagnosis of IOPN is challenging and prone to misdiagnosis.In the first case,the patient underwent a laparoscopic distal pancreatectomy and splenectomy.The surgical procedure spanned 342 minutes,with an estimated intraoperative blood loss of around 100 mL.The patient experienced an uneventful postoperative recovery and was discharged on the 8th postoperative day.For the second case,a laparoscopic pancreaticoduodenectomy was performed.The operation lasted for 431 minutes with an intraoperative blood loss of approximately 50 mL.The patient also demonstrated a favorable postoperative course and was discharged on the 24^(th)postoperative day.Postoperative pathology and immunohistochemistry confirmed the diagnosis of IOPN.No recurrence was observed in either patient after follow-up periods of 8 and 10 months,respectively.CONCLUSION These cases demonstrate that laparoscopic surgery can be considered as one of the treatment options for IOPN of the pancreas.展开更多
Cadmium(Cd^(2+))exhibits pronounced phytotoxicity and poses significant risks to human health through bioaccumulation in agricultural products.This study investigates the mitigative effects of foliar-applied nano-moly...Cadmium(Cd^(2+))exhibits pronounced phytotoxicity and poses significant risks to human health through bioaccumulation in agricultural products.This study investigates the mitigative effects of foliar-applied nano-molybdenum particles(MoNPs)on Cd accumulation and growth rates in rice(Oryza sativa).Our findings demonstrate that MoNPs application effectively alleviates Cd-induced root growth suppression and reduces Cd deposition in root cell walls,through MoNPs-mediated attenuation of Cd-induced elevation of pectin content.Through cross-sectional analysis combined with ROS-specific fluorescent probes revealed a spatial pattern of Cd-induced H_(2)O_(2)accumulation,with strongest signals observed in the apoplastic regions of root elongation and maturation zones,with minimal accumulation in meristematic regions.This oxidative burst was significantly mitigated by MoNPs treatment,which enhanced plasma membrane(PM)-localized respiratory burst oxidase homolog(RBOH)activity via transcriptional upregulation of OsRBOH genes.Furthermore,foliar MoNPs application activated the ascorbate–glutathione(ASA-GSH)cycle through selective upregulation of OsAPXs and OsGRs,enhancing cellular capacity for H_(2)O_(2)detoxification.These coordinated mechanisms collectively suggest that MoNPs treatment offers dual protection against Cd toxicity by 1)reducing Cd bioavailability in plant tissues and 2)counteracting Cd-induced oxidative damage,thereby effectively ameliorating root growth inhibition under Cd stress.展开更多
基金supported by the National Natural Science Foundation of China (NSFC 81902995)the project funded by China Postdoctoral Science Foundation (2018M641936)
文摘Cardiovascular disease is the leading cause of global mortality,with anticoagulant therapy being the main prevention and treatment strategy.Recombinant hirudin(r-hirudin)is a direct thrombin inhibitor that can potentially prevent thrombosis via subcutaneous(SC)and intravenous(IV)administration,but there is a risk of haemorrhage via SC and IV.Thus,microneedle(MN)provides painless and sanitary alternatives to syringes and oral administration.However,the current technological process for the micro mould is complicated and expensive.The micro mould obtained via three-dimensional(3D)printing is expected to save time and cost,as well as provide a diverse range of MNs.Therefore,we explored a method for MNs array model production based on 3D printing and translate it to micro mould that can be used for fabrication of dissolving MNs patch.The results show that r-hirudin-loaded and hyaluronic acid(HA)-based MNs can achieve transdermal drug delivery and exhibit significant potential in the prevention of thromboembolic disease without bleeding in animal models.These results indicate that based on 3D printing technology,MNs combined with r-hirudin are expected to achieve diverse customizableMNs and thus realize personalized transdermal anticoagulant delivery for minimally invasive and long-term treatment of thrombotic disease.
文摘A new skeleton bisabolane-type sesquiterpene curcuminoid,bisabocurcumin（1）,along with 5 known compounds,curcumin（2）, demethoxycurcumin（3）,bidemethoxycurcumin（4）,（1E,4E）-1,5-bis（4-hydroxy-3-methoxyphenyl）-penta-1,4-dien-3-one（5）,and （1E,4E）-1-（4-hydroxy-3-methoxyphenyl）-5-（4-hydroxy phenyl-）-penta-1,4-dien-3-one（6）were isolated from the rhizomes of Curcuma longa L.Their structures were determined on the basis of spectroscopic analysis.Bisabocurcumin（1） is firstly obtained from nature with a new skeleton combined by a bisabolane-type sesquiterpene and a 1,7-diphenylheptanoid through a C-C bond.
基金supported by Key Technologies Research and Development (R & D) Program of Anhui Province of China (1604a0902134)the Key Discipline Team (AKZDXK2015C03 )the Anhui University Provincial Natural Science Research Projects(KJ2016A181 and KJ2016A183)
文摘Static tensile and fatigue tests were performed on shear and tensile self-piercing riveted aluminium-steel structures to evaluate their mechanical and fatigue properties. The influences of the thickness and the strength of the high strength steel on mechanical and fatigue performances were investigated based on the tensile and F-N curves of the joints. The results show that mechanical and fatigue properties of the shear self-piercing riveted joints are much better than those of the tensile self-piercing riveted joints. Mechanical and fatigue performances of the two joints were significantly influenced by the thickness and strength of the steel sheet, and were markedly improved when the thickness of steel sheet increased. The steel strength showed significantly different effects on shear and tensile riveted structures, i. e. , when the steel strength increased, the strength of the shear structure greatly increased while the tensile structure just had a slight increase in the strength. Fatigue failure generally occurred in the sheet materials and the fatigue crack location changed with increasing the sheet thickness and the sheet strength.
文摘Human immunodeficiency virus type 1 （HIV-1） Vpr induces cell death in mammalian and fssion yeast cells, suggesting that Vpr may affect a conserved cellular process. It is unclear, however, whether Vpr-induced yeast cell death mimics Vpr-mediated apoptosis in mammalian cells. We have recently identified a number of Vpr suppressors that not only suppress Vpr-induced cell death in fission yeast, but also block Vpr-induced apoptosis in mammalian cells. These findings suggest that Vpr-induced cell death in yeast may resemble some of the apoptotic processes of mammalian cells. The goal of this study was to develop and validate a fission yeast model system for future studies of apoptosis. Similar to Vpr-induced apoptosis in mammalian cells, we show here that Vpr in fission yeast promotes phosphatidylserine externalization and induces hyperpolarization of mitochondria, leading to changes of mitochondrial membrane potential. Moreover, Vpr triggers production of reactive oxygen species （ROS）, indicating that the apoptotic-like cell death might be mediated by ROS. Interestingly, Vpr induces unique morphologic changes in mitochondria that may provide a simple marker for measuring the apoptotic-like process in fission yeast. To verify this possibility, we tested two Vpr suppressors （EF2 and Hspl6） that suppress Vpr-induced apoptosis in mammalian cells in addition to a newly identified Vpr suppressor （Skpl）. All three proteins abolished cell death mediated by Vpr and restored normal mitochondrial morphology in the yeast cells. In conclusion, Vpr-induced cell death in fission yeast resembles the mammalian apoptotic process. Fission yeast may thus potentially be used as a simple model organism for the future study of the apoptotic-like process induced by Vpr and other proapoptotic agents.
基金provided by the National Natural Science Foundation of China (31901202,31672228)National Distinguished Expert Project (WQ20174400441)+2 种基金the Higher Education Department of Guangdong province (2020KCXTD025)Key Laboratory Project of Guangdong Province (2022B1212010015)the Australian Research Council (DP150101663)。
文摘Aluminum(Al)toxicity poses a significant constraint on field crop yields in acid soils.Zinc finger protein36(ZFP36)is well-documented for its pivotal role in enhancing tolerance to both drought and oxidative stress in rice.This study unveils a novel function of ZFP36 modulated by abscisic acid(ABA)-dependent mechanisms,specifically aimed at alleviating Al toxicity in rice.Under Al stress,the expression of ZFP36significantly increased through an ABA-dependent pathway.Knocking down ZFP36 heightened Al sensitivity,while overexpressing ZFP36 conferred increased resistance to Al stress.Additionally,our investigations revealed a physical interaction between ZFP36 and pyruvate dehydrogenase kinase 1 in rice(OsPDK1).Biochemical assays further elucidated that OsPDK1 phosphorylates ZFP36 at the amino acid site 73–161.Subsequent experiments demonstrated that ZFP36 positively regulates the expression of ascorbate peroxidases(OsAPX1)and OsALS1 by binding to specific elements in their upstream segments in rice.Through genetic and phenotypic analyses,we unveiled that OsPDK1 influences ABA-triggered antioxidant defense to alleviate Al toxicity by interacting with ZFP36.In summary,our study underscores that pyruvate dehydrogenase kinase 1(OsPDK1)phosphorylates ZFP36 to modulate the activities of antioxidant enzymes via an ABA-dependent pathway,influencing tolerance of rice to soil Al toxicity.
基金Supported by the Cooperative Project Between the People's Republic of China and the Republic of Korea
文摘[Objectives] This study was conducted to investigate the effects of different treatments on rooting and germination of Paeonia rockii T. Hong et J.J.Li seeds. [Methods] Different stratification time and temperatures and different GA_3 concentrations and treatment time were set to screen the optimum treatment method for promoting seed rooting and germination. [Results] The rooting rate was the highest when the seeds were subjected to 30 d of stratification treatment in an incubator at 25℃, and treated with 300 mg/L GA_3 for 24 h. Soaking the seeds with 300 mg/L GA_3 for 24 h combined with 30 d of stratification treatment was most beneficial to germination, and the germination rate was over 90%. [Conclusions] This study provides technical guidance and basic information for the rooting and germination of P. rockii seeds.
基金Supported by Science and Technology Project from Binzhou Medical College(BY2007KJ14)
文摘[Objective] This study aimed to identify a maunanase-producing strain isolated from soil. [Method] With kanjac powder as the substrate, a man- nanase-producing dominant strain was iselated from the soil samples collected from Kunyu Mountain by using plate selection method. Sequence analysis of the 16SrDNA fragment of the strain was conducted, and the strain was identified as Bacillus subtilis. Fermentation conditions and enzymatic characteristics were studied preliminarily. [ Result] Experimental result showed that enzyme yield of this strain was different in different medium and in the same medium at different tempera- ture. Enzyme yield of this strain in LB medium was higher when incubated at 27 ℃ than at 30 ℃ ; however, incubation at 30 ℃ was more conducive to the enzyme production than incubation at 27 ℃ in SOC medium. The optimal reaction pH was 7.0 and the optimal reaction temperature was 55 ℃ for enzyme production of this strain. When the temperature was above 55 ℃, enzyme activity declined sharply with the raise of temperature. Under the optimum conditions, enzyme activity could achieve 95.3 U. [ Conclusion] This study provided reference for the industrial application of degradation products of mannan.
基金financial support from the National Natural Science Foundation of China(Grant No.U1637101)The Research Fund of State Key Laboratory of Mechanics and Control for Aerospace Structures(1005-ZAG23011).
文摘As a kind of ionic artificial muscle material,Ionic Polymer-Metal Composites(IPMCs)have the advantages of a low drive current,light weight,and significant flexibility.IPMCs are widely used in the fields of biomedicine,soft robots,etc.However,the displacement and blocking force of the traditional sheet-type Nafion-IPMC need to be improved,and it has the limitation of unidirectional actuation.In this paper,a new type of short side chain Aquivion material is used as the polymer in the IPMC.The cylindrical IPMC is prepared by extrusion technology to improve its actuation performance and realize multi-degree-of-freedom motion.In comparison to the traditional Nafion-IPMC,the ion exchange capacity,specific capacitance,and conductivity of Aquivion-IPMC are improved by 28%,27%,and 32%,respectively,and the displacement and blocking force are improved by 57%and 25%,respectively.The cylindrical actuators can be deflected in eight directions.This indicates that Aquivion,as a polymer membrane for IPMC,holds significant application potential.By designing a cylindrical IPMC electrode distribution,the multi-degree-of-freedom deflection of IPMC can be realized.
基金the National Natural Science Foundation of China(No.61772417,61634004,61602377)Key R&D Program Projects in Shaanxi Province(No.2017GY-060)Shaanxi Natural Science Basic Research Project(No.2018JM4018).
文摘In order to effectively solve the problems of low accuracy,large amount of computation and complex logic of deep learning algorithms in behavior recognition,a kind of behavior recognition based on the fusion of 3 dimensional batch normalization visual geometry group(3D-BN-VGG)and long short-term memory(LSTM)network is designed.In this network,3D convolutional layer is used to extract the spatial domain features and time domain features of video sequence at the same time,multiple small convolution kernels are stacked to replace large convolution kernels,thus the depth of neural network is deepened and the number of network parameters is reduced.In addition,the latest batch normalization algorithm is added to the 3-dimensional convolutional network to improve the training speed.Then the output of the full connection layer is sent to LSTM network as the feature vectors to extract the sequence information.This method,which directly uses the output of the whole base level without passing through the full connection layer,reduces the parameters of the whole fusion network to 15324485,nearly twice as much as those of 3D-BN-VGG.Finally,it reveals that the proposed network achieves 96.5%and 74.9%accuracy in the UCF-101 and HMDB-51 respectively,and the algorithm has a calculation speed of 1066 fps and an acceleration ratio of 1,which has a significant predominance in velocity.
基金the Study on the Mechanism of Action of Dutong and Tiaoshen Acupuncture in Improving Upper Limb Motor Function After Ischemic Stroke based on Functional Near-infrared Spectroscopy Technology(Science and Technology Program of Panyu,2022-Z04-107)。
文摘OBJECTIVE:To take Baihui(GV20)and Fengchi(GB20)targeting inflammatory response to regulate migraine as an example to describe a new method for studying the mechanism of stimulating acupoints.METHODS:The target information of Baihui(GV20)and Fengchi(GB20)was retrieved,and after intersection with migraine,Kyoto Encyclopedia of Genes and Genomes(KEGG),Reactome,and Uni Prot Keywords were used for functional enrichment.After selecting the main pathway,rats were selected and nitroglycerin was used for modeling,and the behavioral scores,inflammatory factors,heme oxygenase 1(HMOX1),protein kinase B(AKT1),signal transducer and activator of transcription 3(STAT3),phosphorylated extracellular signal-regulated kinase 1/2(P-ERK1/ERK2)and other states of the rats in the acupuncture,twisting,and electroacupuncture groups were compared.RESULTS:A total of 135 Baihui(GV20)targets and 27 Fengchi(GB20)targets were collected.A total of 73 target information were obtained after the intersection of these targets in migraine.These 73 targets have three main pathways:hypoxia-inducible factor 1(HIF-1)signaling pathway,signaling by interleukins and inflammatory response.The main targets in the pathway were verified and found that interleukin-1 beta(IL-1β),interleukin-6(IL-6),tumor necrosis factor-alpha(TNF-α)and HMOX1,AKT1,STAT3,P-ERK1/ERK2 can be regulated by Baihui(GV20)and Fengchi(GB20).CONCLUSION:Baihui(GV20)and Fengchi(GB20)can regulate migraine by regulating inflammatory factors and HMOX1,AKT1,STAT3,P-ERK1/ERK2 and other changes in HIF-1 signaling pathway,Signaling by Interleukins and Inflammatory response pathways.Based on systems biology and network pharmacology,and with the model of"acupoint-target-disease",explore the research methods of systematic acupuncture and moxibustion.We believe this is a usable research direction for exploring the mechanism of acupuncture stimulation.
基金financially supported by the National Natural Science Foundation of China(Grant Nos.52275425,52405473,and 52405472)the Natural Science Foundation of Guangdong Province(Grant No.2024A1515010993)。
文摘Surgical electrodes are frequently associated with disadvantages such as high surface adhesion and severe thermal damage to adjacent normal tissues,which threaten operation quality and patient safety.In this study,by mimicking the micromorphology and bio-anti-adhesion of shark skin,we proposed a strategy that utilized nanoscale aluminium oxide(Al_(2)O_(3))films deposited on bioinspired shark skin(BSS)microstructures to design a composite surface(Al_(2)O_(3)@BSS)and integrated it into both flat sides of the surgical electrodes.Micro/nano-manufacturing of the Al_(2)O_(3)@BSS surface was sequentially accomplished using nanosecond laser texturing,atomic layer deposition,and low-temperature annealing,endowing it with excellent blood-repellent properties.Visualisation experiments revealed that the tensile stress gradient of the blood coagulum with increasing thickness under a thermal field prompted it to separate from the Al_(2)O_(3)@BSS surface,resulting in anti-adhesion.Furthermore,it was observed for the first time that Al_(2)O_(3) films could transiently excite discharge along a dielectric surface(DADS)to ablate tissues while suppressing Joule heat,thereby minimising thermal damage.A combination of ex vivo tissue and living mouse experiments demonstrated that the Al_(2)O_(3)@BSS electrodes exhibited optimal comprehensive performance in terms of anti-adhesion,damage minimisation,and drag reduction.In addition,the Al_(2)O_(3)@BSS electrodes possessed remarkable antibacterial efficacy against E.coli and S.aureus.The proposed strategy can meet the extreme application requirements of surgical electrodes to improve operation quality and offer valuable insights for future studies.
基金Supported by the Zhejiang Medical Science and Technology Project,No.2022KY1325 and No.2023KY381Public Welfare Project of Jinhua Science and Technology Plan,No.2023-4-084Major Project of Jinhua Science and Technology Plan,No.2023-3-066.
文摘BACKGROUND Ganglioneuroma is a rare,well-differentiated,slow-growing benign tumor of the peripheral nerves,with surgical resection being the only curative treatment.Surgical resection of ganglioneuromas encasing major blood vessels remains a substantial clinical challenge.Traditionally,these cases often require open abdominal surgery or combined organ resections,and in some instances,the tumors are considered unresectable.Currently,no reports have described the resection of such tumors via laparoscopy.CASE SUMMARY A 35-year-old woman was admitted to our hospital after the incidental discovery of a retroperitoneal space-occupying lesion.Imaging revealed a mass with the celiac axis and superior mesenteric artery passing through it.A neurogenic tumor was suspected,with ganglioneuroma being the most likely diagnosis.Following comprehensive preoperative preparation,the retroperitoneal tumor was resected using a three-dimensional laparoscopy combined with an organ suspension technique.The surgical approach involved incising the tumor along the vascular axis and conducting meticulous,vascular-preserving tumor excision.The operation lasted approximately 458 minutes,with an estimated blood loss of 50 mL.The patient was discharged on the 8th postoperative day.A transient liver injury occurred after surgery but improved rapidly.After 11 months of postoperative follow-up,no complications or tumor recurrence were observed.CONCLUSION This case illustrates the feasibility of minimally invasive laparoscopic resection for retroperitoneal ganglioneuromas encasing major blood vessels.
基金Supported by the Zhejiang Medical Science and Technology Project,No.2022KY1325 and No.2023KY381.
文摘BACKGROUND Intraductal oncocytic papillary neoplasm(IOPN)of the pancreas is an extremely rare pancreatic tumor,with only sporadic cases reported in the literature.IOPN is difficult to diagnose and highly prone to misdiagnosis.IOPN carries a certain risk of progressing to invasive cancer.Surgical resection is the primary treatment for IOPN.According to the existing literature reports,the vast majority of patients with IOPN of the pancreas undergo open surgery,while only one case of laparoscopic surgery have been reported.CASE SUMMARY This report presents two cases of IOPN in elderly female patients,aged 60 and 61.Both patients were asymptomatic,and their pancreatic masses were discovered incidentally.Preoperative diagnosis of IOPN is challenging and prone to misdiagnosis.In the first case,the patient underwent a laparoscopic distal pancreatectomy and splenectomy.The surgical procedure spanned 342 minutes,with an estimated intraoperative blood loss of around 100 mL.The patient experienced an uneventful postoperative recovery and was discharged on the 8th postoperative day.For the second case,a laparoscopic pancreaticoduodenectomy was performed.The operation lasted for 431 minutes with an intraoperative blood loss of approximately 50 mL.The patient also demonstrated a favorable postoperative course and was discharged on the 24^(th)postoperative day.Postoperative pathology and immunohistochemistry confirmed the diagnosis of IOPN.No recurrence was observed in either patient after follow-up periods of 8 and 10 months,respectively.CONCLUSION These cases demonstrate that laparoscopic surgery can be considered as one of the treatment options for IOPN of the pancreas.
基金funding support,including the National Natural Science Foundation of China(W2421108,42377042,32402672)the Basic and Applied Research Foundation of Guangdong province(2023A1515110151,2024A1515140040)+1 种基金the China Postdoctoral Science Foundation(2024 M750949)the Science and Technology Innovation Strategy Fund of Guangdong province(pdjh2024a390).
文摘Cadmium(Cd^(2+))exhibits pronounced phytotoxicity and poses significant risks to human health through bioaccumulation in agricultural products.This study investigates the mitigative effects of foliar-applied nano-molybdenum particles(MoNPs)on Cd accumulation and growth rates in rice(Oryza sativa).Our findings demonstrate that MoNPs application effectively alleviates Cd-induced root growth suppression and reduces Cd deposition in root cell walls,through MoNPs-mediated attenuation of Cd-induced elevation of pectin content.Through cross-sectional analysis combined with ROS-specific fluorescent probes revealed a spatial pattern of Cd-induced H_(2)O_(2)accumulation,with strongest signals observed in the apoplastic regions of root elongation and maturation zones,with minimal accumulation in meristematic regions.This oxidative burst was significantly mitigated by MoNPs treatment,which enhanced plasma membrane(PM)-localized respiratory burst oxidase homolog(RBOH)activity via transcriptional upregulation of OsRBOH genes.Furthermore,foliar MoNPs application activated the ascorbate–glutathione(ASA-GSH)cycle through selective upregulation of OsAPXs and OsGRs,enhancing cellular capacity for H_(2)O_(2)detoxification.These coordinated mechanisms collectively suggest that MoNPs treatment offers dual protection against Cd toxicity by 1)reducing Cd bioavailability in plant tissues and 2)counteracting Cd-induced oxidative damage,thereby effectively ameliorating root growth inhibition under Cd stress.
